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781 results for “Staphylococcus”
Data from: Comparison of the immune response during acute and chronic Staphylococcus aureus infection
Staphylococcus aureus bacteria are able to grow in a planktonic state that is associated with acute infections and in biofilms that are associated with chronic infections. Acute infections, such as skin infections, are often self-limiting. However, chronic infections, such as implant infections, can be difficult to clear and may require surgical intervention. The host immune response may contribute to the different outcomes often associated with these two disease types. We used proteomic arrays and two murine models for an initial, descriptive characterization of the contribution of the host immune response to outcomes of acute versus chronic S. aureus disease. We compared the immune responses between a model of self-limiting skin and soft tissue infection caused by the planktonic form of S. aureus versus a model of surgical mesh implant infection, which we show to be caused by a bacterial biofilm. The significantly altered host cytokines and chemokines were largely different in the two models, with responses diminished by 21 days post-implantation in surgical mesh infection. Because bacterial levels remained constant during the 21 days that the surgical mesh infection was followed, those cytokines that are significantly increased during chronic infection are not likely effective in eradicating biofilm. Comparison of the levels of cytokines and chemokines in acute versus chronic S. aureus infection can provide a starting point for evaluation of the role of specific immune factors that are present in one disease manifestation but not the other.
Data from: Biofilm morphotypes and population structure among Staphylococcus epidermidis from commensal and clinical samples
Bacterial species comprise related genotypes that can display divergent phenotypes with important clinical implications. Staphylococcus epidermidis is a common cause of nosocomial infections and, critical to its pathogenesis, is its ability to adhere and form biofilms on surfaces, thereby moderating the effect of the host's immune response and antibiotics. Commensal S. epidermidis populations are thought to differ from those associated with disease in factors involved in adhesion and biofilm accumulation. We quantified the differences in biofilm formation in 98 S. epidermidis isolates from various sources, and investigated population structure based on ribosomal multilocus typing (rMLST) and the presence/absence of genes involved in adhesion and biofilm formation. All isolates were able to adhere and form biofilms in in vitro growth assays and confocal microscopy allowed classification into 5 biofilm morphotypes based on their thickness, biovolume and roughness. Phylogenetic reconstruction grouped isolates into three separate clades, with the isolates in the main disease associated clade displaying diversity in morphotype. Of the biofilm morphology characteristics, only biofilm thickness had a significant association with clade distribution. The distribution of some known adhesion-associated genes (aap and sesE) among isolates showed a significant association with the species clonal frame, with the exception of. These data challenge the assumption that biofilm-associated genes, such as those on the ica operon, are genetic markers for less invasive S. epidermidis isolates, and suggest that phenotypic characteristics, such as adhesion and biofilm formation, are not fixed by clonal descent but are influenced by the presence of various genes that are mobile among lineages.
Supplementary images and table for the manuscript "A-type natriuretic peptide alters the impact of azithromycin on plank-tonic culture and on (monospecies and binary) biofilms of skin bacteria Kytococcus schroeteri and Staphylococcus aureus"
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Effects of Linezolid on Suppressing Heteroresistant Vancomycin Subpopulations among Heteroresistant Vancomycin Intermediate Staphylococcus aureus (hVISA)
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Metadata of Methicillin Resistant Staphylococcus Aureus (MRSA) sequences in Saudi Arabia
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DeepBacs – Staphylococcus aureus widefield segmentation dataset
<p>Training and test images of live <em>S. aureus</em> cells for the task of cell segmentation.</p> <p>Additional information can be found in the <a href="https://github.com/HenriquesLab/DeepBacs/wiki">github wiki</a>.</p> <p>The example shows the bright field and Nile Red fluorescence image of live <em>S. aureus </em>cells, as well as the manually annotated segmentation mask.</p> <p> </p> <p><strong>Data type</strong>: Paired DIC/fluorescence and segmented mask images</p> <p><strong>Microscopy data type</strong>: 2D widefield images (DIC and fluorescence)</p> <p><strong>Microscope</strong>: GE HealthCare Deltavision OMX system (with temperature and humidity control, 37°C) equipped with an Olympus 60x 1.42NA Oil immersion objective and 2 PCO Edge 5.5 sCMOS cameras (one for DIC, one for fluorescence)</p> <p><strong>Cell type</strong>: <em>S. aureus</em> strain JE2 grown under agarose pads</p> <p><strong>File format</strong>: .tif (16-bit)</p> <p><strong>Image size</strong>: 512 x 512 px² (80 nm/px)<br> <strong>Image preprocessing</strong>: Raw images were manually annotated by drawing ellipses in the NR fluorescence image and segmented images were created using the LOCI plugin (“ROI Map”). For training, images and masks were quartered into four 256 x 256 px² patches.</p> <p> </p> <p><strong>Author(s)</strong>: Pedro Matos Pereira<sup>1,2</sup>, Mariana Pinho<sup>1,3</sup></p> <p><strong>Contact email</strong>: <a href="mailto:pmatos@itqb.unl.pt">pmatos@itqb.unl.pt</a> and <a href="mailto:mgpinho@itqb.unl.pt">mgpinho@itqb.unl.pt</a></p> <p> </p> <p><strong>Affiliation</strong>: </p> <p>1) Bacterial Cell Biology, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal</p> <p>2) ORCID: https://orcid.org/0000-0002-1426-9540</p> <p>3) ORCID: https://orcid.org/0000-0002-7132-8842</p>
Survival of antimicrobial peptide resistant Staphylococcus aureus in mealworm beetles (Tenebrio molitor) with manipulated AMPexpression
<p><span>Antimicrobial peptides (AMP) are essential immune effectors of multicellular organisms. Bacteria can evolve resistance to AMPs. Surprisingly, when used to challenge the insect <em>Tenebrio molitor</em>, <em>Staphylococcus aureus</em> resistant to an abundant beetle AMP (tenecin 1) and also pleiotropically to phagocytosis did not increase host mortality or bacterial load compared to infections with wild-type <em>S. aureus</em>. A possible explanation is that antimicrobial resistance is costly due to collaterally increased sensitivity of AMP-resistant strains to other immune effectors. Here, we studied the sensitivity of a group of AMP-resistant <em>S. aureus</em> strains (which are either resistant to tenecin 1 or a combination of both tenecin 1 and tenecin 2) to other immune effectors such as phenoloxidase (PO) and other AMPs (specifically tenecin 1, tenecin 2 and tenecin 4) <em>in vivo</em>. Using RNAi-based knockdown (KD), we investigated <em>S. aureus in vivo</em> survival in insect hosts lacking particular immune effectors. We found that all except one AMP-resistant strain displayed collateral sensitivity toward phenoloxidase. Moreover, some AMP-resistant mutant strains showed sensitivity to one or more components of the beetle AMP defence cocktail. Our findings are consistent with the idea that resistance to AMPs and phagocytosis does not translate into changes in virulence because it is balanced by collaterally increased sensitivity to other host immune effectors. AMP resistance fails to provide a net survival advantage to <em>S. aureus</em> in a host environment that is dominated by AMPs because of the greater sensitivity to other immune effectors.</span></p>
Figure 2 from: Satria D, Sofyanti E, Wulandari P, Fajarini, Pakpahan SD, Limbong SA (2022) Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC®25175™ and Staphylococcus aureus ATCC®6538™. Pharmacia 69(1): 195-202. https://doi.org/10.3897/pharmacia.69.e77076
Figure 2 Minimum inhibitory zones and biofilm activity of BPCE against S. mutans and S. aureus at different concentrations. Each colour represents the millimetre and percentage from different concentrations of BPCE (mg/mL) in each bacteria.
Figure 4 from: Satria D, Sofyanti E, Wulandari P, Fajarini, Pakpahan SD, Limbong SA (2022) Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC®25175™ and Staphylococcus aureus ATCC®6538™. Pharmacia 69(1): 195-202. https://doi.org/10.3897/pharmacia.69.e77076
Figure 4 The effect of BPCE on calcium and potassium ions leakage from S. mutans and S. aureus at different concentrations. Each colour represents the absorbance from different concentrations of BPCE (mg/mL) in each bacteria.
Figure 3 from: Satria D, Sofyanti E, Wulandari P, Fajarini, Pakpahan SD, Limbong SA (2022) Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC®25175™ and Staphylococcus aureus ATCC®6538™. Pharmacia 69(1): 195-202. https://doi.org/10.3897/pharmacia.69.e77076
Figure 3 The effect of BPCE on membrane intracellular (DNA and protein) leakage from S. mutans and S. aureus at different concentrations. Each colour represents the absorbance from different concentrations of BPCE (mg/mL) in each bacteria.
Figure 1 in Blue and red light photoemitters as approach to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth
Figure 1. Effect of blue and red light -exposure on the growth of S. aureus e P. aeruginosa determined by the number of colonies formed after 24 h incubation. *Statistically significant difference using Mann-Whitney U test (p <0.05) for independent samples.
Dataset and source code for manuscript "Chromosomal gene order defines several structural classes of Staphylococcus epidermidis genomes"
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Data from: Comparative genomics of Methicillin-resistant Staphylococcus aureus ST239: distinct geographical variants in Beijing and Hong Kong
Background: The ST239 lineage is a globally disseminated, multiply drug-resistant hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA). We performed whole-genome sequencing of representative HA-MRSA isolates of the ST239 lineage from bacteremic patients in hospitals in Hong Kong (HK) and Beijing (BJ) and compared them with three published complete genomes of ST239, namely T0131, TW20 and JKD6008. Orthologous gene group (OGG) analyses of the Hong Kong and Beijing cluster strains were also undertaken. Results: Homology analysis, based on highest-percentage nucleotide identity, indicated that HK isolates were closely related to TW20, whereas BJ isolates were more closely related to T0131 from Tianjin. Phylogenetic analysis, incorporating a total of 30 isolates from different continents, revealed that strains from HK clustered with TW20 into the 'Asian clade', whereas BJ isolates and T0131 clustered closely with strains of the 'Turkish clade' from Eastern Europe. HK isolates contained the typical φSPβ-like prophage with the SasX gene similar to TW20. In contrast, BJ isolates contained a unique 15 kb PT1028-like prophage but lacked φSPβ-like and φSA1 prophages. Besides distinct mobile genetic elements (MGE) in the two clusters, OGG analyses and whole-genome alignment of these clusters highlighted differences in genes located in the core genome, including the identification of single nucleotide deletions in several genes, resulting in frameshift mutations and the subsequent predicted truncation of encoded proteins involved in metabolism and antimicrobial resistance. Conclusions: Comparative genomics, based on de novo assembly and deep sequencing of HK and BJ strains, revealed different origins of the ST239 lineage in northern and southern China and identified differences between the two clades at single nucleotide polymorphism (SNP), core gene and MGE levels. The results suggest that ST239 strains isolated in Hong Kong since the 1990s belong to the Asian clade, present mainly in southern Asia, whereas those that emerged in northern China were of a distinct origin, reflecting the complexity of dissemination and the dynamic evolution of this ST239 lineage.
Linezolid Alone or Combined With Carbapenem Against Methicillin-resistant Staphylococcus Aureus (MRSA) in Ventilator-associated Pneumonia
ClinicalTrials.gov study NCT01356472. IPD Sharing: Not stated. Countries: 0. Publications: 5.
A Phase 3 Telavancin Staphylococcus Aureus (S. Aureus) Bacteremia Trial
ClinicalTrials.gov study NCT02208063. IPD Sharing: NO. Countries: 1. Publications: 0.
Phase 2a Study of CG400549 for the Treatment of cABSSSI Caused by Methicillin-resistant Staphylococcus Aureus
ClinicalTrials.gov study NCT01593761. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Evaluation of Ceftaroline Fosamil Versus a Comparator in Adult Subjects With Community-acquired Bacterial Pneumonia (CABP) With Risk for Methicillin-resistant Staphylococcus Aureus
ClinicalTrials.gov study NCT01645735. IPD Sharing: Not stated. Countries: 8. Publications: 0.
Combination Antibiotic Therapy for Staphylococcus Aureus Bacteremia
ClinicalTrials.gov study NCT07376889. IPD Sharing: YES. Countries: 1. Publications: 0.
Ceftobiprole in the Treatment of Resistant Staphylococcus Aureus Skin and Skin Structure Infections
ClinicalTrials.gov study NCT00210899. IPD Sharing: Not stated. Countries: 0. Publications: 1.
Prevention of Recurrent Infections Caused by Community Acquired Staphylococcus Aureus (CA-SA) in Children
ClinicalTrials.gov study NCT00901316. IPD Sharing: Not stated. Countries: 1. Publications: 0.
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