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1,044 results for “pcr”
Data from: Development of a PCR-RFLP assay to identify Drosophila melanogaster among field-collected larvae
The fruit fly Drosophila melanogaster is a model organism to study several aspects of metazoan biology. Most of the work has been conducted in adult fruit flies, including laboratory and field-derived specimens, but Drosophila melanogaster larvae recently became a valuable model to better understand animal physiology, development or host-microbe interactions. While adult flies can be easily assigned to a given Drosophila species based on morphological characteristics, such visual identification is more intricate at the larval stage. This could explain the limited number of studies focusing on larvae, especially field-derived samples. Here, we developed a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) assay that discriminates D. melanogaster from other ecologically relevant Drosophila species at the larval stage. The method, which targets the cytochrome oxidase I (COI) gene, was validated using laboratory-derived larvae from seven D. melanogaster populations originating from different geographic areas as well as six Drosophila species. We further validated this PCR-RFLP assay in a natural context, by identifying wild larvae collected in two locations in France. Notably, among all PCR-RFLP profiles that matched the D. melanogaster species, 100% were correctly identified, as confirmed by COI sequencing. In summary, our work provides a rapid, simple and accurate molecular tool to identify D. melanogaster from field-collected larvae.
Data from: Detection of airborne genetically modified maize pollen by real-time PCR
The cultivation of genetically modified (GM) crops has raised in the European Union and other parts of the world numerous concerns about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dispersal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GM maize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semi-urban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GM pollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this ''GM pollen monitoring by bioaerosol sampling and PCR screening" approach might represent an aid in managing the co-cultivation of GM and non-GM crops, especially in the surveillance of GM-free areas, centres of origin, and natural reserves.
FIGURE 2 in Identification of Planococcus ficus and Planococcus citri (Hemiptera: Pseudococcidae) by PCR-RFLP of COI gene
FIGURE 2. RFLP analysis with HinfI restriction enzyme of COI gene. Digested products were separated on 2% agarose gels stained with ethidium bromide. Lanes: 1-8, Pl citri; 9-13, Pl ficus; M, 100 bp ladder.
FIGURE 1 in Identification of Planococcus ficus and Planococcus citri (Hemiptera: Pseudococcidae) by PCR-RFLP of COI gene
FIGURE 1. COI gene restriction patterns of Ps. longispinus (L), Pl. citri (C) and Pl. ficus (F) generated by BspPI, HinfI and SspI.
FIGURES 11–15 in Recovery of mitochondrial DNA for systematic studies of Pentatomoidea (Hemiptera: Heteroptera): successful PCR on early 20 century dry museum specimens
FIGURES 11–15. Trace files (electropherograms) showing the fragment (between 230/240bp and 340/350bp) of the subunit 16S. 11, Parastrachiidae: Dismegistus binotatus; 12–13, Thyreocoridae: Strombosoma impictum (12), Galgupha difficilis (13); 14–15. Dinidoridae: Coridius nepalensis (14), Megymenum brevicorne (15).
FIGURES 16–17 in Recovery of mitochondrial DNA for systematic studies of Pentatomoidea (Hemiptera: Heteroptera): successful PCR on early 20 century dry museum specimens
FIGURES 16–17. Trace files (electropherograms) showing the fragment (between 240bp and 350bp) of the subunit 16S. 16, Dinidoridae: Megymenum parallelum; 17, Tessaratomidae: Tessaratoma quadrata.
FIGURES 1–5 in Recovery of mitochondrial DNA for systematic studies of Pentatomoidea (Hemiptera: Heteroptera): successful PCR on early 20 century dry museum specimens
FIGURES 1–5. Trace files (electropherograms) showing the same fragment (between 120bp and 230bp) of the subunit 12S. 1– 2, Cydnidae: Chilocoris assmuthi (1), Linospa orbicularis (2); 3, Parastrachiidae: Dismegistus binotatus; 4–5, Thyreocoridae: Strombosoma impictum (4), Galgupha difficilis (5).
FIGURE 1 in Experimental population genetics in insects: inheritance of ISSR-PCR bands in an artificial population
FIGURE 1. The NJ trees of the 0/1 matrices of the ISSR-PCR fingerprints. Numbers at internal branches indicate bootstrap support for the five families, if>50%.
Input data for PCR-GLOBWB-RF (30 arcmin)
<p>Input data for the 30 arcmin hybrid global streamflow modelling framework described in 'Global streamflow modelling using process-informed machine learning'. Contains the location of the selected 2286 stations and their respective observed discharge (GRDC), input meteorology, input catchment parameters and the state variables and streamflow from the uncalibrated PCR-GLOBWB.</p>
Outputs of PCR-GLOBWB-RF (30 arcmin)
<p>Output data for the 30 arcmin hybrid global streamflow modelling framework described in 'Global streamflow modelling using process-informed machine learning'. Each subsection contains the files for every one of the 5 random subsamples.</p>
Hydrographs from validation subsets of PCR-GLOBWB-RF (30 arcmin)
<p>Plots showing hydrographs and flow duration curves (observed discharge, uncalibrated PCR-GLOBWB discharge and hybrid modelled discharge, 1979-2019) and respective residuals at all stations used for cross-validation (5 subsamples).</p> <p>A selected number of these plots were used in figure 7 in the paper, to show that the hybrid approach can improve (most of the time) or worsen the performance of uncalibrated PCR-GLOBWB. Here we store all the plots produced during cross-validation, to satisfy any potential curiosity of the reader. Plot numbers reflect stations from the Global Runoff Data Centre (GRDC). Note that the hybrid approach can produce a continuous synthetic streamflow time-series, irrespective of the availability of data at a particular station.</p> <p>A description of the hybrid methodology used to produce these time-series can be found in the paper 'Global streamflow modelling using process-informed machine learning', by Michele Magni, Edwin H. Sutanudjaja, Youchen Shen and Derek Karssenberg.</p>
Fig. 3 in Establishment of conventional PCR and real-time PCR assays for accurate, rapid and quantitative authentication of four mistletoe species
Fig. 3. Sensitivity and amplification efficiency of qPCR assay using species-specific primers and serial dilutions of standard DNA. PCR cycling (A, C, E, G) and melting curve (B, D, F, H) analysis of primers specific for V. coloratum using VC-F3/R4 (A, B); T. sutchuenensis var. duclouxii using TS-F3/R4-1 (C, D), K. japonica using KJ-F5-1/R4 (E, F), and L. tanakae using LT-F5/R5-1 primers (G, H). Arrows indicate the amplification curves (A, C, E, G) of serial dilutions of standard DNA with NTC. Threshold was determined according to the Rotor-Gene Q series software (version 2.3.1) manual.
Fig. 2 in Establishment of conventional PCR and real-time PCR assays for accurate, rapid and quantitative authentication of four mistletoe species
Fig. 2. Sensitivity of multiplex PCR assay in conventional PCR. M: 100 bp DNA ladder. VC: V. coloratum. TS: T. sutchuenensis var. duclouxii. KJ: K. japonica. LT: L. tanakae. (+) means samples mixed different species used in PCR template. Hyphens and arrows indicate the sizes of DNA ladder and PCR products, respectively.
Fig. 4 in Establishment of conventional PCR and real-time PCR assays for accurate, rapid and quantitative authentication of four mistletoe species
Fig. 4. Analysis of commercial mistletoe products sold as leached tea in the Korean market using multiplex PCR assay. M: 100 bp DNA ladder. C1, VC_JM; C2, VC_GY; C3, TS_HR1; C4, TS_GC2; C5, KJ_SC1; C6, KJ_PS; C7, LT_GW; and C8, LT_JC (positive control). Lanes 1–16: Commercial food and herbal medicine products distributed as authentic mistletoe. Hyphens and arrows indicate the sizes of DNA ladder and PCR products, respectively. *, inauthentic products whose identity did not match the description on the label; **, inauthentic products whose identity matched the description on the label.
Clinical Application of PCR-based Diagnosis of Community-onset Viral Respiratory Infections.
ClinicalTrials.gov study NCT01133782. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Effect of Stress Ball and Breathing Exercises on Reducing Fear and Pain Associated with PCR Testing
ClinicalTrials.gov study NCT05250544. IPD Sharing: NO. Countries: 1. Publications: 1.
Point of Care Polymerase Chain Reaction (PCR) Diagnostics of Respiratory Tract Infections in General Practice
ClinicalTrials.gov study NCT06120153. IPD Sharing: NO. Countries: 1. Publications: 6.
Fast Identification of Pathogen in the Setting of Pneumonia Using Multiplex PCR
ClinicalTrials.gov study NCT01858974. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Impact of Influenza/RSV PCR Point-of-care Testing in the Emergency Medical Service.
ClinicalTrials.gov study NCT04048369. IPD Sharing: Not stated. Countries: 1. Publications: 7.
MRD Monitoring by Digital Droplet PCR in the Early Period After Allo-HSCT to Predict Patients at High Risk of Relapse
ClinicalTrials.gov study NCT06000306. IPD Sharing: Not stated. Countries: 1. Publications: 1.
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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