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Dataset results
2,014 results for “Vitamin D”
Vitamin D receptor (VDR) regulates epidermal stem cells and cutanous wound healing
GEO Series GSE68730. Mus musculus. 6 samples. Type: Expression profiling by array.
Active forms of vitamin D as preventive and therapeutic agents in cutaneous melanoma: review and perspective
GEO Series GSE248660. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
The role of vitamin D in the transcriptional program of human pregnancy.
GEO Series GSE86200. Homo sapiens. 60 samples. Type: Expression profiling by array.
Common and personal target genes of the micronutrient vitamin D in primary immune cells from human peripheral blood
GEO Series GSE179621. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
Common and private vitamin D target genes of human primary immune cells: a personalized approach
GEO Series GSE156124. Homo sapiens. 30 samples. Type: Expression profiling by high throughput sequencing.
Vitamin D inhibition of metastasis and oxidative stress in osteosarcoma
GEO Series GSE220948. Homo sapiens. 8 samples. Type: Expression profiling by high throughput sequencing.
Oral vitamin D for the attenuation of sunburn
GEO Series GSE86406. Homo sapiens. 41 samples. Type: Expression profiling by array.
Effect of vitamin D on gene expression in human alveolar type II cells
GEO Series GSE46749. Homo sapiens. 8 samples. Type: Expression profiling by array.
Effect of Vitamin D on gene expression by CaCO2 cells
GEO Series GSE444. Homo sapiens. 10 samples. Type: Expression profiling by array.
Effects of vitamin D supplementation on alveolar macrophage gene expression
GEO Series GSE56583. Homo sapiens. 44 samples. Type: Expression profiling by array.
Vitamin D Receptor (VDR) ChIP-seq reported in "Vitamin D receptor and STAT3 cooperate to TET2-mediated acquisition of tolerogenesis"
GEO Series GSE145584. Homo sapiens. 9 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Effects of vitamin D on histone modifications H3K27ac and H3K4me3 in THP-1 cells
GEO Series GSE107851. Homo sapiens. 13 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Vitamin D deficiency during development permanently alters liver cell composition and function.
GEO Series GSE194417. Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.
Analysis of vitamin D response element binding protein target genes reveals a role for vitamin D in osteoblast mTOR signaling
GEO Series GSE22523. Homo sapiens. 8 samples. Type: Expression profiling by array.
Transcriptomics of vitamin D treatment effects in Mycobacterium tuberculosis-infected THP-1 cells
GEO Series GSE57028. Homo sapiens. 14 samples. Type: Expression profiling by array.
Patient-derived metastatic melanoma cells treated with vitamin D and anticancer drugs
<ol> <li><strong>Immunofluorescence staining</strong></li> </ol> <p>For immunofluorescence analyses cells isolated from malignant melanoma tumour were seeded in 8-well chamber slides, fixed with 4% paraformaldehyde (PFA) for 10 min and permeabilized for 5 min in 0.2% Triton X100 in PBS. Slides were washed three times with PBS and incubated with 1% BSA for 30 min at RT. Following blocking appropriate primary antibody in 0.1% BSA (Thermo Fisher Scientific: PMEL (HMB45) MA5-13232, 1:40; Melan-A MA5-15237, 1:100 or MA5-32217, 1:200; Cell Signaling Technology: FAP Cat. No. 66562, 1:100) was applied and the slides were incubated in humidified chamber at 4 °C overnight. Subsequently slides were washed three times in PBS, and incubated with secondary antibody (ThermoFisher Scientific either Alexa Fluor® 488 conjugate goat anti rabbit IgG A11008 or Alexa Fluor® 594 conjugate donkey anti mouse IgG A21203) solution in PBS for 1 h at RT in dark. Following three washing steps in PBS the slides were counterstained with 4′,6′-diamidinio-2-phenylindole (DAPI). Images were collected with a fluorescence microscope Olympus cell Vivo IX 83. For a-SMA (alpha-smooth muscle actin) immunofluorescence analyses the procedure of staining with Melan A was proceeded to the appropriate secondary antibodies as described above. Subsequently antibody against a-SMA PE‑conjugated (R&D Systems: IC1420P, 1:50) was applied to the specimen for an additional 30 min in humidified chamber at 4 °C. Following three washing steps in PBS the slides were counterstained with DAPI. Images were collected with a fluorescence microscope Olympus cell Vivo IX 83 as Z‑stacks.</p> <ol> <li><strong>SRB viability assay</strong></li> </ol> <p>The viability of cells isolated from metastatic malignant melanoma tissues treated with 1,25(OH)<sub>2</sub>D<sub>3</sub>, vemurafenib or both was assessed with sulforhodamine B (SRB). The cells were seeded in 96-well plates at 2,000 of cells per well density and left overnight. Subsequently the cells were treated simultaneously with serial dilutions of vemurafenib (3,15-200 nM) and 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM or 1 µM concentration for 72 h. Cells were fixed with 10% trichloroacetic acid for 1 h at 4°C, washed with distilled water and stained for 15 min with 0.4% SRB solution (Sigma–Aldrich; Merck KGaA) in 1 % acetic acid. SRB dye was solubilized using a solution of 10 mM buffered Tris Base (pH 10.5) and absorbance measured at 570 nm using an Epoch spectrophotometer (BioTek, Winooski, USA). The relative IC50 value was calculated as the mid‑point between no inhibition and the maximum observed decrease in proliferation (n=6).</p> <ol> <li><strong>Proliferation rate assay</strong></li> </ol> <p>Proliferation rate analysis of the cells isolated from malignant melanoma tissues treated with 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration and cediranib at 1000 nM concentration was carried out as a live imaging with Olympus cell Vivo IX 83, equipped with CO<sub>2</sub> incubator and enables for stable cell cultivation at 37°C, controlled humidity and 5% CO<sub>2</sub> during the whole 72 h experiment. Results were calculated with the Olympus cell Vivo IX 83 software with use of TruAI technology, normalized with 1.0 at the beginning of the experiment (n = 4).</p> <ol> <li><strong>Wound closure rate</strong></li> </ol> <p>The experiment was carried out as a live imaging with Olympus cell Vivo IX 83 and cell migration process was observed during 72 h incubation. The cells isolated from malignant melanoma tissues were seeded on a 8-well chamber slide (1.0 × 10<sup>5</sup> cells per well). A mechanical wound was created by scraping the confluent monolayer of cells with a 200 µL pipette tip. Cediranib at 1000 nM concentration was added to the cells pretreated with 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration for 24 h. The cell free area was calculated as a percentage closure relative to original size [(wound area in mm<sup>2</sup>)*100/(original wound area in mm<sup>2</sup>)] with use of TruAI technology of the Olympus cellSens software (n = 4).</p> <ol> <li><strong>Mitochondrial membrane potential</strong></li> </ol> <p>Patients-derived cells were seeded on an 8-well chamber slide (2.0 × 10<sup>4</sup> cells per well). After 24 h, cells were stained with MitoTracker™ Red CM-H<sub>2</sub>Xros (Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 70 nM concentration for 20 min at 37°C, and washed two times with PBS. Cells were pretreated with 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration for 24h. Subsequently cediranib at 1000 nM concentration was added to the cells in a fresh medium and mitochondria were observed for additional 24 h. The experiment was carried out as a live imaging with Olympus cell Vivo IX 83. Results were calculated as an area fraction ROI (“the mitochondria”) normalized with 1.0 at the beginning of the experiment with the Olympus cell Vivo IX 83 software, (n = 3 - 4).</p> <ol> <li><strong>Measurement of melanoma bioenergetics</strong></li> </ol> <p>Patients-derived cells were seeded at a density of 20,000 cells per well in an Agilent Seahorse microplate and cultured at 37°C in 5% CO<sub>2</sub> until reaching 80% confluency in 80 μL culture medium per well. Subsequently cells were incubated for 24 h with vemurafenib at 200 nM concentration or cediranib at 1000 nM concentration with or without 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration. Additionally, selected cells were also pretreated with 1,25(OH)<sub>2</sub>D<sub>3</sub> at 100 nM concentration for 24 h and then incubated with the chosen anticancer drug at appropriate concentration for 24h. The Cell Mito Stress Test was performed as described by Franczak M. et al. (Franczak, M.; Kutryb-Zajac, B.; El Hassouni, B.; Giovannetti, E.; Granchi, C.; Minutolo, F.; Smolenski, R.T.; Peters, G.J. The effect of lactate dehydrogenase-A inhibition on intracellular nucleotides and mitochondrial respiration in pancreatic cancer cells. <em>Nucleosides Nucleotides Nucleic Acids </em><strong>2022</strong>, 1-11, doi:10.1080/15257770.2022.2031215.). The medium was changed before analysis for the XFp assay medium with 10 mM glucose, 2 mM glutamine and 1 mM pyruvate (Agilent, US). During the assay 1.5 μM oligomycin, 1 μM carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) and 0.5 μM rotenone with antimycin were added sequentially for the Cell Mito Stress Test. The XFp sensor cartridge, with compounds used in the test, was incubated at 37 °C in the non-CO<sub>2</sub> incubator for 15 minutes before the assay and plate with cells for 45 minutes. Each analysis was performed by the Agilent Seahorse XFp Analyzer (Agilent, Santa Clara, CA, USA). The oxygen consumption rate (OCR) and the mitochondrial respiration parameters were calculated with the software provided with the Agilent Seahorse XFp Analyzer (n = 2-5).</p>
Calcium, Vitamin D and Metformin to Treat Insulin Resistance in Obese African American Adolescent Females
ClinicalTrials.gov study NCT01107808. IPD Sharing: Not stated. Countries: 0. Publications: 0.
Vitamin D and Immunity: Photosynthesis Versus Supplementation
ClinicalTrials.gov study NCT03609970. IPD Sharing: NO. Countries: 0. Publications: 0.
Vitamin D and Disease Activity in Ulcerative Colitis
ClinicalTrials.gov study NCT02717897. IPD Sharing: NO. Countries: 0. Publications: 0.
Prevalence of Serum-Vitamin D Abnormalities, Periodontitis and Anaemia in Patients With Melasma
ClinicalTrials.gov study NCT03241381. IPD Sharing: NO. Countries: 0. Publications: 0.
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International Brain Laboratory public data
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OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.