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4 results for “14C primary production”
Primary production estimates from 14C uptake (in situ), determined by the incorporation of inorganic carbon into particulate organic carbon (POC) due to photosynthesis at selected light levels from CCE LTER process cruises in the California Current System, 2006 - 2021 (ongoing).
Primary productivity samples of seawater are taken each day shortly before noon on the CTD rosette up-cast during the CCE Process crusies (since 2006, ongoing). Light penetration below the surface is estimated from the Secchi disk depth. Niskin bottles from depths with ambient light intensities corresponding to light levels simulated by on-deck incubators are identified and sampled. Primary production is estimated from 14C uptake using this simulated in situ technique (followed by filtering) by which the assimilation of dissolved inorganic carbon by phytoplankton yields a measure (in µg/L/day) of the rate of photosynthetic primary production (particulate organic carbon, POC) at selected light levels in the euphotic zone within the CCE study area.
Time-series measurements of size fractionated primary production (14C-assimilation) in the subtropical North Pacific Ocean
<p>Filter size-fractionated (>0.2-3 μm) particulate 14C-based rates of primary production were measured at six discrete depths (5, 25, 45, 75, 100, and 125 m) throughout the euphotic zone. Seawater samples from each depth were subsampled into triplicate 30-mL polycarbonate centrifuge tubes from a pre-dawn cast, inoculated with 70 µL of NaH14CO3-, then incubated over the full photoperiod (~12-14 hours) on a floating in situ array at the corresponding depths where the water was collected. At the end of the incubation period (after sundown), 25 mL of each sample was vacuum-filtered first onto a 25-mm diameter 3-μm pore size polycarbonate membrane, then the filtrate was vacuum-filtered onto a 25-mm diameter 0.2-μm pore size polycarbonate membrane filter. The rates reported here are for the >0.2-3 μm size fraction.</p>
Time series measurements of 14C-based primary production (>0.2 um) in the subtropical North Pacific Ocean
<p>Over a 3-year period (April 2010-April 2013), we measured primary production from vertical profiles at near-monthly time scales in the subtropical North Pacific Ocean. Production measurements were based on <sup>14</sup>C-assimilation into >0.2 um plankton biomass. Seawater for the productivity measurements was collected from predawn CTD hydrocasts into acid-cleaned 500-ml polycarbonate bottles. A total of four replicate 500 ml bottles were subsampled per depth and each bottle was spiked with ~1.85 MBq <sup>14</sup>C-bicarbonate. One hundred milliliters from one replicate per depth was immediately vacuum filtered through a polycarbonate filter as a time zero blank. These filters were placed in 20 ml glass scintillation vials and stored at -20oC until shore-based laboratory processing. The remaining three bottles were hung on a free-drifting array, deployed before dawn, and incubated at their initial collection depths throughout the photoperiod (typically 11-13 hours). After sunset the array was recovered, and 100 ml subsamples of all bottles were filtered under gentle vacuum onto 0.2 um polycarbonate filters that were then placed in scintillation vials and frozen. The total radioactivity added to each sample bottle was determined by subsampling 250 µl aliquots into scintillation vials containing 500 µl of β-phenylethylamine. At the shore-based laboratory, filters were acidified, passively vented, and the resulting radioactivity was determined using liquid scintillation counting.</p>
Data for: Phytoplankton primary productivity: a dual-incubation approach for direct comparison of photosystem II photosynthetic flux (JVPII) and 14C-fixation experiments
<p>Data summary and LabSTAF raw data from manuscript "Phytoplankton primary productivity: a dual-incubation approach for direct comparison of photosystem II photosynthetic flux (JVPII) and 14C-fixation experiments" by <span>Nina Schuback, Kevin Oxborough, Mary Burkitt-Gray, </span><span>Patricia López-García, Matthew D. Patey, </span><span>Emily Hammermeister, Alan Wright, C. Mark Moore</span></p>
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