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907 results for “A549”
Time-lapse 3D confocal microscopy videos of mitochondrial dynamics in human alveolar epithelial cells (A549-DsRed) infected with Mycobacterium marinum (Mmar) strains
<div>The dataset consists of time-lapse, 3D confocal images of mitochondrial dynamics in human alveolar epithelial cells (A549-DsRed) infected with Mycobacterium marinum (Mmar) strains. Images were captured at 60X magnification in an environmental chamber at 35°C for live-cell imaging. Host cell mitochondria were labeled with red fluorescent protein (RFP) and infected with both wildtype (wt) and ESAT-6 operon knockout mutant labeled with green fluorescent protein (GFP) at MOI of 100 for 24 hours at 35°C. Infected cells were identified and analyzed to explore the effect of pathogenic mycobacteria on mitochondrial morphology over time.</div> <div> </div> <div>More details available in this preprint: <a href="https://doi.org/10.48550/arXiv.2411.06035">https://doi.org/10.48550/arXiv.2411.06035</a></div>
Minimal data set for: Air-liquid interface exposure of A549 human lung cells to characterize the hazard potential of a gaseous bio-hybrid fuel blend
<p>This minimal data set presents the values behind the means and standard deviation for the publication entitled: "Air-liquid interface exposure of A549 human lung cells to characterize the hazard potential of a gaseous bio-hybrid fuel blend"</p>
Well overviews of A549 spheroids at varying cell plating density and Matrigel concentrations
<p>Above images depict well overviews of 5-day old A549 spheroid culture at varied cell plating density (1000,2000 and 3000 cells per well) and Matrigel density (10, 20 and 25 microliters). These were imaged using Perkin Elmer Opera Phenix High Content Screening System and were stained with a nuclear marker (Hoescht 33342) and an actin marker phalloidin/Alexa568.</p>
Well overviews of A549 spheroids at varying cell plating density and Matrigel concentrations
<p>Above images depict well overviews of 5-day old A549 spheroid culture at varied cell plating density (1000,2000 and 3000 cells per well) and Matrigel density (10, 20 and 25 microliters). These were imaged using Perkin Elmer Opera Phenix High Content Screening System and were stained with a nuclear marker (Hoescht 33342) and an actin marker phalloidin/Alexa568.</p>
Virtual screening on SARS-CoV-2 Nsp14_testing of 5474 hits in a Nsp14 biochemical assay and an A549-hACE2 SARS-CoV-2 infected cells
<p> </p> <p>This report describes the most relevant results of virtually screening the Janssen Pharmaceutica compound collection for potential activity against SARS-CoV-2 Nsp14 and confirmation of potential hits in a SARS-CoV-2 Nsp10/Nsp14 biochemical exonuclease assay and in a A549-hACE2 cell-based anti-SARS-CoV-2 assay.</p>
Gene expression profile at both bulk and single cell level for heterogeneous cell type mixtures of A375, HAP1, THP1, HEK239FT, K562, and A549
<p>We used poly-A bulk RNA sequencing and single-cell RNA sequencing using mixtures of well-characterized purified cell lines to better understand measurement noise between technologies. A375, HAP1, THP1, HEK239FT, K562, and A549 cells were mixed in various proportions to generate four in vivo cell mixtures that mimic tissues. Mixtures of 6, 4, 2, and 1 of the cell types are respectively denoted 6ct, 4ct, 2ct, and 1ct. In the included zip, directories are labeled using this notation, followed by a list of cell types in the mixture after an underscore. In each directory, bulk and single cell count data is included.</p>
NSD3-Short Promotes Migration of A549 Lung Cancer Cells
<p><strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong></p> <p><strong>Exp025 Objective:</strong> In a previous experiment (exp024), we observed an increase in E-cadherin expression in response to knockdown of the short isoform of NSD3. To determine if this change is functionally relevant, we performed wound healing assays to measure any corresponding alteration in the migratory potential of A549 lung epithelial cancer cells.</p>
NSD3-Short Represses E-cadherin Expression in A549 Lung Epithelial Cancer Cells
<p><strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong></p> <p><strong>Exp024 Objective: </strong>In a previous experiment (exp023), we show that siRNA-mediated knockdown of NSD3 increases expression of E-cadherin, a marker of epithelial cell identity. To determine which isoform of NSD3 is involved in promoting epithelial to mesenchymal transition, I have designed siRNA that targets either the long or short isoform and again use E-cadherin expression as a marker. Additionally, I have started using A549 lung epithelial cancer cells (https://www.atcc.org/Products/All/CCL-185) as my primary model. This cell line proliferates faster and is more amenable to <em>in vitro</em> experimentation than the H1299 cell line I was using previously.</p>
Fig. 7 in Cardiac glycosides from the roots of Streblus asper Lour. and their apoptosis-inducing activities in A549 cells
Fig. 7. Western blot analysis of caspases-3, -8, -9, Bax, and Bcl-2 in A549 cells with compound 3 (1 μM) for 8 and 24 h. The results are from one representative experiment among three runs, showed similar patterns. Significantly different (***p <0.001) compared with the initial model group.
Fig. 6 in Cardiac glycosides from the roots of Streblus asper Lour. and their apoptosis-inducing activities in A549 cells
Fig. 6. Induction of apoptosis against A549 cells treated with compound 3. (PI: propidium iodide; N: negative control; C: compound 3) (A) Apoptotic cells in the compound 3 (1 μM) treated A549 cells at 12, 24, and 48 h. (B) Negative, control group DMSO at 48 h. (C) Early apoptosis and late apoptosis of A549 cells compared with negative group at 48 h. Each value is the mean of three experiments. Significant different (***p <0.001) compared with control model group.
Fig. 5 in Cardiac glycosides from the roots of Streblus asper Lour. and their apoptosis-inducing activities in A549 cells
Fig. 5. Detection of cell shrinkage induced by compound 3, and fragmentation by nuclear staining with Hoechst 33342 in A549 cells, which were cultured for 24 h.
Fig. 4 in Cardiac glycosides from the roots of Streblus asper Lour. and their apoptosis-inducing activities in A549 cells
Fig. 4. Experimental ECD and calculated ECD spectrum of 6 in MeOH.
Fig. 3 in Cardiac glycosides from the roots of Streblus asper Lour. and their apoptosis-inducing activities in A549 cells
Fig. 3. Selected ROESY correlations of compounds 1–6.
Fig. 2. Selected HMBC and 1 H– 1 H in Cardiac glycosides from the roots of Streblus asper Lour. and their apoptosis-inducing activities in A549 cells
Fig. 2. Selected HMBC and 1 H– 1 H COSY correlations of compounds 1–6.
Fig. 1 in Cardiac glycosides from the roots of Streblus asper Lour. and their apoptosis-inducing activities in A549 cells
Fig. 1. The structures of 1–9 isolated from the roots of S. asper.
Antiviral effect of Hyunggaeyungyo-tang on A549 cells infected with human coronavirus
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total RNA-seq from A549 (ENCSR155ZTO)
GEO Series GSE219516. Homo sapiens. 5 samples. Type: Expression profiling by high throughput sequencing.
m7G methylation by METTL1 regulates let-7 microRNAs [BoRed-Seq & m7G-RIP-Seq A549]
GEO Series GSE112181. Homo sapiens. 14 samples. Type: Non-coding RNA profiling by high throughput sequencing; Other.
Knockdown of CUL3 and PTEN in A549 lung adenocarcinoma cells
GEO Series GSE68869. Homo sapiens. 12 samples. Type: Expression profiling by array.
ChIP-seq from A549 (ENCSR897MYK)
GEO Series GSE92148. Homo sapiens. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
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