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ShareScore release 0.9.0
Dataset results
13 results for “Actin polymerization”
Reconstitution of phase-separated signaling clusters and actin polymerization on supported lipid bilayers
<p>Liquid–liquid phase separation driven by weak interactions between multivalent molecules contributes to the cellular organization by promoting the formation of biomolecular condensates. At membranes, phase separation can promote the assembly of transmembrane proteins with their cytoplasmic binding partners into micron-sized membrane-associated condensates. For example, phase separation promotes clustering of nephrin, a transmembrane adhesion molecule, resulting in increased Arp2/3 complex-dependent actin polymerization. In vitro reconstitution is a powerful approach to understanding phase separation in biological systems. With a bottom-up approach, we can determine the molecules necessary and sufficient for phase separation, map the phase diagram by quantifying de-mixing over a range of molecular concentrations, assess the material properties of the condensed phase using fluorescence recovery after photobleaching (FRAP), and even determine how phase separation impacts downstream biochemical activity. Here, we describe a detailed protocol to reconstitute nephrin clusters on supported lipid bilayers with purified recombinant protein. We also describe how to measure Arp2/3 complex-dependent actin polymerization on bilayers using fluorescence microscopy. These different protocols can be performed independently or combined as needed. These general techniques can be applied to reconstitute and study phase-separated signaling clusters of many different receptors or to generally understand how actin polymerization is regulated at membranes.</p>
Reconstitution of phase-separated signaling clusters and actin polymerization on supported lipid bilayers
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Microscopy and biophysical data for: Synthetic control of actin polymerization and symmetry breaking in active protocells
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DNA sequences for: Synthetic control of actin polymerization and symmetry breaking in active protocells
<p>Non-linear biomolecular interactions on membranes drive membrane remodeling crucial for biological processes including chemotaxis, cytokinesis, and endocytosis. The complexity of biomolecular interactions, their redundancy, and the importance of spatiotemporal context in membrane organization impede understanding of the physical principles governing membrane mechanics. Developing a minimal in vitro system that mimics molecular signaling and mem- brane remodeling while maintaining physiological fidelity poses a significant challenge. Inspired by chemotaxis, we reconstructed chemically regulated actin polymerization inside vesicles, guiding membrane self-organization. An external, undirected chemical input induced directed actin polymerization and membrane deformation uncorrelated with upstream biochemical cues, suggesting symmetry breaking. A biophysical model incorporating actin dynamics and membrane mechanics proposes that uneven actin distributions cause non-linear membrane deformations, consistent with experimental findings. This protocellular system illuminates the interplay between actin dynamics and membrane shape during symmetry breaking, offering insights into chemotaxis and other cell biological processes.</p>
DNA sequences for: Synthetic control of actin polymerization and symmetry breaking in active protocells
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Pulses of RhoA Signaling Stimulate Actin Polymerization and Flow in Protrusions to Drive Collective Cell Migration
<p>In animals, cells often move as collectives to shape organs, close wounds, or—in the case of disease—metastasize. To accomplish this, cells need to generate force to propel themselves forward. The motility of singly migrating cells is driven largely by an interplay between Rho GTPase signaling and the actin network. Whether cells migrating as collectives use the same machinery for motility is unclear. Using the zebrafish posterior lateral line primordium as a model for collective cell migration, we find that active RhoA and myosin II cluster on the basal sides of the primordium cells and are required for primordium motility. Positive and negative feedbacks cause RhoA and myosin II activities to pulse. These pulses of RhoA signaling stimulate actin polymerization at the tip of the protrusions and myosin II-dependent actin flow and protrusion retraction at the base of the protrusions, and deform the basement membrane underneath the migrating primordium. This suggests that RhoA-induced actin flow on the basal sides of the cells constitutes the motor that pulls the primordium forward, a scenario that likely underlies collective migration in other—but not all—contexts.</p>
Multiscale reorganization of the genome following DNA damage facilitates chromosome translocations via nuclear actin polymerization
GEO Series GSE183059. Mus musculus. 44 samples. Type: Other.
Mitochondrial dysfunction, disruption of F-actin polymerization, and transcriptomic alterations in zebrafish larvae exposed to trichloroethylene
GEO Series GSE72918. Danio rerio. 8 samples. Type: Expression profiling by array.
Control of plasma membrane-associated actin polymerization specifies the pattern of the cell wall in xylem vessels.
GEO Series GSE286145. Arabidopsis thaliana. 12 samples. Type: Expression profiling by high throughput sequencing.
MKL1 deficiency results in a severe neutrophil motility defect due to impaired actin polymerization
GEO Series GSE134644. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
Actin polymerization by WASp limits nuclear envelope rupture and inflammation in macrophages
GEO Series GSE285024. Mus musculus. 10 samples. Type: Expression profiling by high throughput sequencing.
Actin polymerization by WASp limits nuclear envelope rupture and inflammation in macrophages I
GEO Series GSE285174. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Characterization of open chromatin sensitive to actin polymerization using DSP-MNase-DNA-seq
GEO Series GSE257539. Homo sapiens. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.