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3 results for “Amine metabolites”
Amine metabolites in pigs fed a diet with spray dried plasma protein as functional protein source
<p><span>We evaluated the effects of diets formulated with either soybean meal (SBM) as a reference protein source or SDPP in pigs. Blood amine profiles were analysed to evaluate the effects of the diets at a systemic level. <span>Blood samples were collected via the ear-vein for plasma preparation at at dissection days (d28-29) after the morning meal ingestion. </span></span></p> <p><span>For plasma, blood samples were collected in sterile Vacuette tubes containing lithium-heparin and immediately centrifuged at 3,000x g for 10 min at 4°C and plasma was extracted. Plasma were stored at -80°C for further analysis on levels ofsystemic amine metabolite profiles. </span></p> <p>The protocol outlined in the following publication was used for detecting plasma amine levels:</p> <ul> <li>Noga MJ, Dane A, Shi S, Attali A, van Aken H, Suidgeest E, et al. Metabolomics of cerebrospinal fluid reveals changes in the central nervous system metabolism in a rat model of multiple sclerosis. <span><span><span>Metabolomics. 2012;8(2):253-63.</span></span></span></li> <li><span><span><span>van der Kloet FM, Bobeldijk I, Verheij ER, Jellema RH. </span></span></span>Analytical Error Reduction Using Single Point Calibration for Accurate and Precise Metabolomic Phenotyping. Journal of Proteome Research. 2009;8(11):5132-41.</li> </ul>
Feeding black soldier fly larva to replace soybean meal in growing pigs – responses in the amine metabolites in blood
<p>Insect meals from black soldier fly (<em>Hermetia illucens</em>; BSF) larvae as dietary protein source have the ability to deliver nutrients, particularly dietary amino acids (AA) and could provide functional properties that positively supports animal health and productivity. More knowledge, however, is needed to assess the impact of BSF based diet on gut and animal health. Sixteen male pigs with an average initial body weight of 34.9 ± 3.4 kg were randomly assigned to groups fed for three weeks with iso-caloric and iso-proteinaceous experimental diets prepared with either soybean meal (SBM) as reference protein source or with BSF, as single source of dietary protein. At the end of the feeding trial, blood plasma were collected to study the changes at systemic level in plasma amine metabolites as an effect of the experimental diet.</p>
Amines and lipids metabolites in blood plasma and saliva samples in pigs.
<p>The dataset presented in here is generated in a project named "<strong>Effects of sanitary and health status on amino acid and energy metabolism of growing-finishing pigs.</strong>" The metabolomics data from two samples types in pigs were generated in collaboration with Metabolomics Facility Leiden, The Netherlands and Wageningen Livestock Research, The Netherlands. This collaboration was realized and funded by Enabling Technology Hotels programme, ZonMW, NWO, The Netherlands (<strong>project number: 435005015</strong>). </p> <p>Targeted quantification of metabolites in two metabolomic platforms covering amines and oxidative stress metabolites in the blood and saliva samples in pigs. The samples were collected from a feeding trial. Briefly, After weaning, i.e., at week 4, pigs were fed a starter (4-9 weeks), grower (9-14 weeks), and finisher (14-22 weeks) diet containing either starch or fat as an energy source. At week 9, before the pigs were fed the grower diet, blood plasma and saliva samples were collected from the pigs (n=6) and the animals were stratified according to different hygiene conditions. At week 14, i.e., before the pigs received the finisher diet, and at week 22, i.e., at the end of this experiment, blood plasma and saliva samples were collected from the pigs (n=6) in the cohort receiving a diet with a different energy source under contrasting sanitary status. </p> <p>Targeted quantification of metabolites in two metabolomic platforms covering amines and oxidative stress metabolites in the blood and saliva samples in pigs. The number of identified metabolites are shown in Table 1.</p> <p><strong>Table 1</strong>: <strong>Number of identified amines and lipids metabolites in blood plasma and saliva samples in pigs.</strong> </p> <table> <tbody> <tr> <td> <table align="center"> <tbody> <tr> <td> <p> </p> </td> <td> <p>Data reported as</p> </td> </tr> <tr> <td> </td> <td> <p>Peak areas<sup>1</sup></p> </td> <td> <p>Relative response ratios<sup>4</sup></p> </td> </tr> <tr> <td> <p> </p> </td> <td> <p>Confidence<sup>2</sup></p> </td> <td> <p>Caution<sup>3</sup></p> </td> <td> <p>Confidence</p> </td> <td> <p>Caution</p> </td> </tr> <tr> <td> <p><em>Amines</em></p> </td> <td> </td> <td> </td> <td> </td> <td> </td> </tr> <tr> <td> </td> <td> </td> <td> </td> <td> </td> <td> </td> </tr> <tr> <td> <p>Blood plasma</p> </td> <td> <p>not required</p> </td> <td> <p>not required</p> </td> <td> <p>58</p> </td> <td> <p>2</p> </td> </tr> <tr> <td> <p>Saliva</p> </td> <td> <p>not required</p> </td> <td> <p>not required</p> </td> <td> <p>52</p> </td> <td> <p>5</p> </td> </tr> <tr> <td> <p><em>Lipids </em></p> <p><em>(low pH)</em></p> </td> <td> </td> <td> </td> <td> </td> <td> </td> </tr> <tr> <td> </td> <td> </td> <td> </td> <td> </td> <td> </td> </tr> <tr> <td> <p>Blood plasma</p> </td> <td> <p>47</p> </td> <td> <p>17</p> </td> <td> <p>47</p> </td> <td> <p>17</p> </td> </tr> <tr> <td> <p>Saliva</p> </td> <td> <p>18</p> </td> <td> <p>34</p> </td> <td> <p>52</p> </td> <td> <p>11</p> </td> </tr> <tr> <td> </td> <td> </td> <td> </td> <td> </td> <td> </td> </tr> <tr> <td> <p><em>Lipids </em></p> <p><em>(High pH)</em></p> </td> <td> </td> <td> </td> <td> </td> </tr> <tr> <td> </td> <td> </td> <td> </td> <td> </td> <td> </td> </tr> <tr> <td> <p>Blood plasma</p> </td> <td> <p>24</p> </td> <td> <p>25</p> </td> <td> <p>24</p> </td> <td> <p>25</p> </td> </tr> <tr> <td> <p>Saliva</p> </td> <td> <p>28</p> </td> <td> <p>17</p> </td> <td> <p>28</p> </td> <td> <p>17</p> </td> </tr> </tbody> </table> <p> </p> </td> </tr> </tbody> </table> <p></p> <p><sup>1</sup> For the lipid platform, large variations in internal standard were observed between study samples. This could be due to the difference in matrix effect between the study samples, i.e., blood plasma and saliva. It is known that the matrix effect varies significantly depending on the origin of the samples and is influenced by phenotypic characteristics such as species, age, and gender. Therefore, peak areas were provided as an additional data set that can be used as input data for downstream metabolomics analysis.</p> <p><sup>2</sup> Metabolite signaling complied with the acceptance criteria of RSDqc <15%.</p> <p><sup>3</sup> Metabolite signaling did not comply with the acceptance criteria of our quality control i.e. of RSDqc <15%, but they present RSDs up to 30%.</p> <p><em><sup>4</sup> </em>target area/ISTD area; unit free<em>.</em> </p> <p>Available data-set:</p> <p>-Four different signaling lipids data-set: 1) peak areas for plasma samples, 2) peak area ratios (metabolite to ISTD) for plasma samples, 3) peak areas for saliva samples, and 4) peak area ratios (metabolite to ISTD) for saliva samples.</p> <p> - Two signalling amine data-set: 1) peak area ratios (metabolite to ISTD) for plasma samples, and 2) peak area ratios (metabolite to ISTD) for saliva samples.</p> <p> </p>
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