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59 results for “Azoles”
Mass spectrometry dataset for: "Discovery of Nostatin A, an azole containing sactipeptide with prominent cytostatic activity and pro-apoptotic activity"
<p>MSn mass spectrometry dataset used in: <strong>Discovery of nostatin A, an azole-containing sactipeptide with prominent cytostatic and pro-apoptotic activity.</strong> Kateřina Delawská*, Jan Hájek*, Kateřina Voráčová*, Marek Kuzma, Jan Mareš, Kateřina Vicková, Alan Kádek, Dominika Tučková, Filip Gallob, Petra Divoká, Martin Moos, Stanislav Opekar, Lukas Koch, Kumar Saurav, David Sedlák, Petr Novák, Petra Urajová, Jason Dean, Radek Gažák, Timo J.H. Niedermeyer, Zdeněk Kameník, Petr Šimek, Andreas Villunger and Pavel Hrouzek. <em>Org. Biomol. Chem.</em> (2025). doi:<a title="Link to landing page via DOI" href="https://doi.org/10.1039/D4OB01395F">10.1039/D4OB01395F</a></p> <p> </p> <p><strong>Description:</strong></p> <p>MSn mass spectrometry elucidation of the molecular structure of nostatin A, a bioactive peptide isolated from Nostoc sp. cyanobacteria.</p> <p><strong>Sample and data processing:</strong></p> <p><em><strong>1) FTICR data:</strong></em><br>Experiments were performed using a 15T SolariX XR Fourier-transform ion cyclotron resonance mass spectrometer (ESI-FTICR MS; Bruker Daltonics, Billerica, MA, USA) equipped with infrared multiple photon dissociation (IRMPD). All data were acquired using 2 µl/min direct infusion of NosA dissolved at 10 µM in 60% acetonitrile acidified with 0.1% formic acid. Ion fragmentation was performed using IRMPD inside the ICR cell. For this a Diamond C-30A CO2 laser (Coherent, Santa Clara, CA, USA) resonating at 10.6 µm was custom-coupled to the SolariX FTICR MS and laser pulses were precisely timed in synchronization with the ICR pulse sequence. Further MS3 fragmentation experiments were performed using in source collisional fragmentation (isCID) followed by quadrupole isolation and subsequent collision induced fragmentation of particular fragment ions of interest. Detailed parameters (ESI and MS settings) are stored inside the metadata of the individual data files as well as described in the resulting publication. Spectral peaks were also exported in plain m/z vs intensity XY text files from Bruker Data Analysis 5.1.</p> <p><em><strong>2) qTOF data:</strong></em><br>HPLC-HRMS experiment was performed using a Dionex UltiMate 3000 HPLC system (Thermo Scientific, Sunnyvale, CA, USA) coupled with a diode array detector (DAD) connected to the Bruker Impact HD mass spectrometer equipped with an electrospray ionization (ESI) source (ESI-HRMS; Bruker, Billerica, MA, USA). The separations were performed on a C18 column (Phenomenex Kinetex C18, 150 × 4.6 mm, 2.6 μm) eluted with water (A)/acetonitrile (B) gradient (0 min 15%, 1 min 15%, 20 min 100%, 25 min 100%, 30 min 15%, 33min 15% of B) at a constant flow rate of 0.6 mL/min. Both solvents were acidified with 0.1% formic acid. Fragmentation energy was set to 87eV and 35eV for 1+ and 2+ Nostatin A, respectively. Detailed parameters (ESI and MS settings) are stored in the metadata of the individual data file as well as described in the resulting publication.</p> <p>*other variants of Nostatin bearing different acyl chains on proline residue were detected in crude extract only (C33_nosA_LCMS_2217.d) and not purified.</p> <p>Raw Bruker DataAnalysis .d files, which otherwise behave as folders, were compressed with the TAR algorithm implemented in the 64-bit Total Commander 11.02. </p>
Azole resistance mechanisms and population structure of Aspergillus fumigatus on retail plant products
<p><em>Aspergillus fumigatus </em>is a ubiquitous saprotroph and human-pathogenic fungus that is life-threatening to the immunocompromised. Triazole-resistant <em>A. fumigatus</em><em> </em>was found in patients without prior treatment with azoles, leading researchers to conclude that resistance had developed in agricultural environments where azoles are used against plant pathogens. Previous studies have documented azole-resistant <em>A. fumigatus </em>across agricultural environments, but few have looked at retail plant products. Our objectives were to determine if azole-resistant <em>A. fumigatus </em>is prevalent<em> </em>in retail plant products produced in the United States (U.S.), as well as to identify the resistance mechanism(s) and population genetic structure of these isolates. Five hundred twenty-five isolates were collected from retail plant products and screened for azole resistance. Twenty-four isolates collected from compost, soil, flower bulbs, and raw peanuts were pan-azole resistant. Resistant isolates had the TR<sub>34</sub>/L98H, TR<sub>46</sub>/Y121F/T289A, G448S, and H147Y <em>cyp51A </em>alleles, all known to underly pan-azole resistance, as well as WT alleles, suggesting that non-cyp51A-mechanisms contribute to pan-azole resistance in some isolates. Minimum spanning networks showed two lineages containing isolates with TR alleles or the F46Y/M172V/E427K allele, and discriminant analysis of principle components (DAPC) identified three primary clusters. This is consistent with previous studies detecting three clades of <em>A. fumigatus</em> and identifying pan-azole-resistant isolates with TR alleles in a single clade. We found pan-azole resistance in U.S. retail plant products, particularly compost and flower bulbs, which indicates the risk of exposure to these products for susceptible populations and that highly resistant isolates are likely distributed worldwide on these products.</p>
Azole resistance mechanisms and population structure of Aspergillus fumigatus on retail plant products
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Data from: Asexual sporulation facilitates adaptation: the emergence of azole resistance in Aspergillus fumigatus
Understanding the occurrence and spread of azole resistance in Aspergillus fumigatus is crucial for public health. It has been hypothesized that asexual sporulation, which is abundant in nature, is essential for phenotypic expression of azole-resistance mutations in A. fumigatus facilitating subsequent spread through natural selection. Furthermore, the disease aspergilloma is associated with asexual sporulation within the lungs of patients and the emergence of azole resistance. This study assessed the evolutionary advantage of asexual sporulation by growing the fungus under pressure of one of five different azole fungicides over seven weeks and by comparing the rate of adaptation between scenarios of culturing with and without asexual sporulation. Results unequivocally show that asexual sporulation facilitates adaptation. This can be explained by the combination of more effective selection because of the transition from a multicellular to a unicellular stage, and by increased mutation supply due to the production of spores, which involves numerous mitotic divisions. Insights from this study are essential to unravel the resistance mechanisms of sporulating pathogens to chemical compounds and disease agents in general, and for designing strategies that prevent or overcome the emerging threat of azole resistance in particular.
Data from: Phase-dependent differential in vitro and ex vivo susceptibility of Aspergillus flavus and Fusarium keratoplasticum to azole antifungals
<p>Fungal keratitis (FK) is an invasive infection of the cornea primarily associated with <em>Aspergillus</em> and <em>Fusarium</em> species. FK is treated empirically with a limited selection of topical antifungals with varying levels of success. Though clinical infections are typically characterized by a dense network of mature mycelium, traditional models used to test antifungal susceptibility of FK isolates exclusively evaluate susceptibility in fungal cultures derived from asexual spores known as conidia. The purpose of this study was to characterize differences in fungal response when topical antifungal treatment is initiated at progressive phases of fungal development. We compared efficacy of voriconazole and luliconazole against <em>in vitro</em> cultures of <em>A. flavus</em> and <em>F. keratoplasticum</em> at 0, 24, and 48 h of fungal development. Data from this assay is included here, reported as end-point absorbance values. A porcine cadaver corneal model was used to compare antifungal efficacy of voriconazole and luliconazole in <em>ex vivo</em> tissue cultures of <em>A. flavus</em> and <em>F. keratoplasticum</em> at 0, 24, and 48 h of fungal development. Data from this assay may be found here, reported as pixel counts of the determined area.</p>
Azole-echinocandin Combination Therapy for Invasive Aspergillosis
ClinicalTrials.gov study NCT04876716. IPD Sharing: Not stated. Countries: 2. Publications: 1.
Incidence of Oral Candidiasis, Prevalence of C. Dubliniensis in HIV Patients and In-vitro Azole Susceptibility
ClinicalTrials.gov study NCT00692783. IPD Sharing: Not stated. Countries: 1. Publications: 6.
ATCF (Azole Therapy in Cystic Fibrosis)
ClinicalTrials.gov study NCT01576315. IPD Sharing: Not stated. Countries: 2. Publications: 3.
Azole Susceptibility and Resistance Mechanisms Surveys in Pathogenic Fungi by the CARST-fungi (2023-2024)
ClinicalTrials.gov study NCT06969703. IPD Sharing: YES. Countries: 1. Publications: 2.
PCR Based Detection of Azole Resistance in A. Fumigatus to Improve Patient Outcome.
ClinicalTrials.gov study NCT03121235. IPD Sharing: UNDECIDED. Countries: 1. Publications: 9.
Data from: Phase-dependent differential in vitro and ex vivo susceptibility of Aspergillus flavus and Fusarium keratoplasticum to azole antifungals
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Data from: Asexual sporulation facilitates adaptation: the emergence of azole resistance in Aspergillus fumigatus
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Data from: Relevance of heterokaryosis for adaptation and azole-resistance development in Aspergillus fumigatus
Aspergillus fumigatus causes a range of diseases in humans, some of which are characterized by fungal persistence. A. fumigatus, being a generalist saprotroph, may initially establish lung colonisation due to its physiological versatility and subsequently adapt through genetic changes to the human lung environment and antifungal treatments. Human lung-adapted genotypes can arise by spontaneous mutation and/or recombination and subsequent selection of the fittest genotypes. Sexual and asexual spores are considered crucial contributors to the genetic diversity and adaptive potential of aspergilli by recombination and mutation supply respectively. However, in certain Aspergillus diseases, such as cystic fibrosis and chronic pulmonary aspergillosis, A. fumigatus may not sporulate but persist as a network of fungal mycelium. During azole therapy, such mycelia may develop patient-acquired resistance and become heterokaryotic by mutations in one of the nuclei. We investigated the relevance of heterokaryosis for azole-resistance development in A. fumigatus. We found evidence for heterokaryosis of A. fumigatus in patients with chronic Aspergillus diseases. Mycelium from patient-tissue biopsies segregated different homokaryons, from which heterokaryons could be reconstructed. Whereas all variant homokaryons recovered from the same patient were capable of forming a heterokaryon, those from different patients were heterokaryon-incompatible. We furthermore compared heterokaryons and heterozygous diploids constructed from environmental isolates with different levels of azole resistance. When exposed to azole, the heterokaryons revealed remarkable shifts in their nuclear ratio, and the resistance level of heterokaryons exceeded that of the corresponding heterozygous diploids.
Prophylaxis Trial of Posaconazole Versus Standard Azole Therapy for Neutropenic Patients (Study P01899)
ClinicalTrials.gov study NCT00044486. IPD Sharing: Not stated. Countries: 0. Publications: 1.
Anti-mold Azole in the Prophylaxis for Invasive Fusariosis
ClinicalTrials.gov study NCT02714504. IPD Sharing: NO. Countries: 1. Publications: 0.
Efflux Pump Mediated Azole Resistance in Candida Albicans
ClinicalTrials.gov study NCT03659162. IPD Sharing: UNDECIDED. Countries: 0. Publications: 10.
Data from: Relevance of heterokaryosis for adaptation and azole-resistance development in Aspergillus fumigatus
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Potent synergistic interactions between lopinavir and azole antifungal drugs against emerging multidrug-resistant Candida auris
GEO Series GSE148341. Candidozyma auris. 4 samples. Type: Expression profiling by high throughput sequencing.
Exploring comparative and transcriptomic analyses to unveil mechanisms of resistance to azoles dependent and independent of CgPdr1 in Candida glabrata clinical isolates
GEO Series GSE166841. Nakaseomyces glabratus. 14 samples. Type: Expression profiling by array.
AtrR is an essential determinant of azole resistance in Aspergillus fumigatus
GEO Series GSE123446. Aspergillus fumigatus Af293. 14 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
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