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10 results for “Azospirillum brasilense”
Figure 1 in Characterization of glutamine synthetase from the ammonium-excreting strain HM053 of Azospirillum brasilense
Figure 1. Transferase activity of glutamine synthetase. (A) Transferase activity of wild-type glutamine synthetase in the absence and presence of magnesium as well as with snake venom phosphodiesterase treatment; (B) Transferase activity of P347L glutamine synthetase in the absence and presence of magnesium, and with snake venom phosphodiesterase treatment. The activity of GS is expressed in µmol γ-glutamyl-hydroxamate.min-1.mg protein-1, given that the absorbance of 530 nm of 1 µmolγ-glutamylhydroxamate was 0.054. The total activity was determined in the absence of Mg2+ (-Mg2+) and the non-adenylylated (active) fraction was determined in the presence of 60 mM Mg2+ (+Mg2+). Samples were incubated at 30 ºC for 0, 10, 30 and 60 min before measuring activity. SVP-treated GS samples (+ SVP) were incubated with snake venom phosphodiesterase. GS activity reactions contained 3 µg of protein.
Figure 3 in Characterization of glutamine synthetase from the ammonium-excreting strain HM053 of Azospirillum brasilense
Figure 3. Prediction of the structure of glutamine synthetase from the mutant P347L. (A) Prediction of the P347L-GS structure. The amino acid marked in pink corresponds to leucine in strain HM053; (B) b1) Prediction structure of wild-type GS from amino acid 346 to 361. b2) Prediction structure of P347L-GS from amino acid 346 to 361. b3) Alignment of prediction structures of wildtype GS and P347L GS from amino acid 346 to 361. The amino acid marked in blue corresponds to the proline that is mutated in strain HM053. The amino acid marked in pink is leucine that replaced proline in the mutated amino acid in strain HM053.
Figure 2 in Characterization of glutamine synthetase from the ammonium-excreting strain HM053 of Azospirillum brasilense
Figure 2. Western blot assays of glutamine synthetase after treatment with snake venom phosphodiesterase. Samples (~ 0.3 µg GS protein) were separated by SDS‐PAGE followed by Western blotting with an anti‐GS antibody. A) Wild-type glutamine synthetase; B) P347L glutamine synthetase. Lane 1: GS after 0 min of incubation at 30 ºC without any treatment; lanes 2 to 5: GS after 0, 10, 30 and 60 min incubation at 30 ºC with snake venom phosphodiesterase. Lane 6: GS after 60 min incubation at 30 ºC without treatment.
Screening the maize rhizobiome for consortia that improve Azospirillum brasilense root colonization and plant growth outcomes
<p>Data corresponds to results from: <em>Barua N, Clouse KM, Ruiz Diaz DA, Wagner MR, Platt TG and Hansen RR (2023) Screening the</em> <em>maize rhizobiome for consortia that improve Azospirillum brasilense root colonization and plant growth outcomes. Front. Sustain. Food Syst. 7:1106528. doi: 10.3389/fsufs.2023.1106528</em></p>
Changes in the transcriptome of Azospirillum brasilense, sp7 in response to ethylene
GEO Series GSE179776. Azospirillum brasilense. 6 samples. Type: Expression profiling by high throughput sequencing.
Small RNA Sequencing of the Plant Growth Promoting Bacterium Azospirillum brasilense Sp245
GEO Series GSE117764. Azospirillum baldaniorum. 2 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Azospirillum brasilense alters root system architecture through both auxin-dependent and -independent pathways
GEO Series GSE192383. Arabidopsis thaliana. 12 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome profile of vegetative and cyst cells states of Azospirillum brasilense sp7
GEO Series GSE104188. Azospirillum brasilense. 6 samples. Type: Expression profiling by high throughput sequencing.
Metataxonomics 16S rDNA of maize bulk and rhizospheric soil samples with seed bacterization with Azospirillum brasilense Ab-V5 in a dilution-to-extinction of native microbial diversity
<p><strong>The beneficial plant-microbes association has been widely explored with the purpose of understanding the mechanisms involved in this interaction, in order to develop bioinoculants for agriculture aiming at sustainability and reduction of chemical inputs use. Plant growth promoting bacteria (PGPBs) have been reported as an interesting alternative to mineral fertilizers for a range of crops. The genus Azospirillum has shown potential in plant growth promotion for maize. However, the lack of holistic comprehension about PGPBs-plant-native soil microbiota can lead to inconsistency of results in field conditions. Recent ecological theories revealed that plant microbiomes are organized as microbial hubs with keystone species and helpers. The objective of this work is to characterize the microbial community associated with a commercial maize genotype (30A37PW) under influence of the potential keystone PGPB A. brasilense Ab-v5 to support the original development of a synthetic minimum microbiome including this strain as keystone for plant growth promotion. Aiming to understand the PGPB-plant-microbiome system, we conducted a greenhouse experiment in which the synergistic effect of Ab-V5 and soil native microbial community were evaluated through soil and rhizosphere metataxonomics, metagenomics and plant phenomics. Our results revealed that maize seed bacterization with the PGPB Ab-V5 promotes plant growth. This effect is boosted by the interaction with specific microbial groups present in the soil native community. The strain Ab-V5 persists in the maize rhizosphere until 25 days after sowing. In order to understand which microbial groups are important during the interaction Ab-V5-plant-native soil microbial community, 16S rDNA metataxonomics and metagenomics analysis will be conducted in maize soil and rhizosphere samples. This unprecedented omics approach for the holistic view of the complex system composed by inoculant-plant-microbiota and data integration will allow the development of new strategies for optimization of plant biostimulants recommendation with enhanced efficiency for establishment in the field. </strong></p>
Maize phenotypic traits evaluated under synergistic influence of native soil microbial communities (dilution-to-extinction) and biostimulant Azospirillum brasilense Ab-V5
<p>Maize phenotypic traits evaluated under synergistic influence of native soil microbial communities (dilution-to-extinction) and biostimulant Azospirillum brasilense Ab-V5.</p>
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