Find research datasets worth reusing

Fig. 4. Amino acid sequences alignment between TCS1 and candidate N in Discovery and Biochemical Characterization of N-methyltransferase Genes Involved in Purine Alkaloid Biosynthetic Pathway of Camellia gymnogyna Hung T.Chang (Theaceae) from Dayao Mountain

Fig. 4. Amino acid sequences alignment between TCS1 and candidate N-methyltransferase genes (GCS1, GCS2, and GCS3).

Fig. 2 in Discovery and Biochemical Characterization of N-methyltransferase Genes Involved in Purine Alkaloid Biosynthetic Pathway of Camellia gymnogyna Hung T.Chang (Theaceae) from Dayao Mountain

Fig. 2. Purine alkaloid content in different leaf positions of C. gymnogyna and other tea plants. Bud, 1st, 2nd, 3rd, 4th, and 5th represent the apical bud, first leaf, second leaf, third leaf, and fourth leaf, respectively. Total purine alkaloid concentration is the sum of Tb, Cf, and Tc. Asterisk indicates not detected. A lack of sufficient Kucha bud samples prohibited the analysis of that component. Data represent the mean value ± SD of independent experiments performed in triplicate. Data with the same letter and numeric above SD bar in each column are not significantly different from each other at P ≦ 0.05. Data with alphabet are significantly different from the data with numeric above SD bar.

Fig. 6. N in Discovery and Biochemical Characterization of N-methyltransferase Genes Involved in Purine Alkaloid Biosynthetic Pathway of Camellia gymnogyna Hung T.Chang (Theaceae) from Dayao Mountain

Fig. 6. N-methyltransferase gene expression patterns in different leaf positions of C. gymnogyna and other tea plants. Bud, 1st, 2nd, 3rd, 4th, and 5th represent the apical bud, first leaf, second leaf, third leaf, and fourth leaf, respectively.

Fig. 5 in Discovery and Biochemical Characterization of N-methyltransferase Genes Involved in Purine Alkaloid Biosynthetic Pathway of Camellia gymnogyna Hung T.Chang (Theaceae) from Dayao Mountain

Fig. 5. Phylogenetic tree of N-methyltransferase amino acid sequences. Substrates of the enzymes are indicated in parentheses. The following amino acid sequences were subjected to sequence alignment: TCS1, AB031280; CKCS, MN163829; TCS1d, KT215399; TCS1f, KT215398; TCS1e, KT215397; CkTcS, MN163831; CkTbS, MN163830; ICS1, AB056108; PCS1, AB207817; ICS2, AB207816; PCS2, AB207818; TCS2, AB031281; CkCS1, AB362884; CjCS1, AB297451; CgCS1, AB362882; CgCS2, AB362883; ClCS1, AB362885; CsSAMT, MG459470. Abbreviations of substrates are as follows: 7-mX, 7-methylxanthine; Tb, theobromine; Tc, theacrine; XR, xanthosine; CsSAMT as a outgroup.

Fig. 1 in Discovery and Biochemical Characterization of N-methyltransferase Genes Involved in Purine Alkaloid Biosynthetic Pathway of Camellia gymnogyna Hung T.Chang (Theaceae) from Dayao Mountain

Fig. 1. Main metabolic pathway for the biosynthesis and biodegradation of Cf. SAM = S-adenosyl- L -methionine, SAH = S-adenosyl- L -homo-cysteine. (b). Numbers (I, II, III, and IV) in (b) correspond to the reactions I, II, III, and IV in (a), respectively. TCS1 and CkCS have broad substrate specificities and catalyze the conversion of 7-mX to Cf via Tb [reactions II and III in (a)].

Fig. 3 in Discovery and Biochemical Characterization of N-methyltransferase Genes Involved in Purine Alkaloid Biosynthetic Pathway of Camellia gymnogyna Hung T.Chang (Theaceae) from Dayao Mountain

Fig. 3. Gene annotation. (a) Venn diagram; (b) The results of volcano plots of differential genes between the experimental groups; (c) GO annotation of DEGs; (d) The top 20 KEGG pathways of DEGs.

Fig. 5 in L-DOPA synthesis in Mucuna pruriens (L.) DC. is regulated by polyphenol oxidase and not CYP 450/tyrosine hydroxylase: An analysis of metabolic pathway using biochemical and molecular markers

Fig. 5. The amplicons generated using degenerate primer approach. (a) Lane M-DNA ladder, Lane 1–250 bp amplicon generated using MTH –F and MTH-R primer pairs of TH gene (b) Lane M-DNA ladder, Lane 1 and 2–800 bp amplicon using primers deduced from the peptide sequence derived through LCMS/MS.

Fig. 4 in L-DOPA synthesis in Mucuna pruriens (L.) DC. is regulated by polyphenol oxidase and not CYP 450/tyrosine hydroxylase: An analysis of metabolic pathway using biochemical and molecular markers

Fig. 4. Effect of enzyme inhibitors on L-DOPA production in callus cultures of M. pruriens was estimated using HPTLC. The culture without inhibitor was treated as negative control and cultures with different concentration of the inhibitor were the test samples. (Control-untreated, C = Cimetidine at 1.98 μM and 19.8 μM; Q = Quinidine at 1.46 μM and 14.6 μM; A = L-ascorbic acid at 567 μM and 851 μM; K = Kojic acid at 703 μM and 1055 μM).

Fig. 3 in L-DOPA synthesis in Mucuna pruriens (L.) DC. is regulated by polyphenol oxidase and not CYP 450/tyrosine hydroxylase: An analysis of metabolic pathway using biochemical and molecular markers

Fig. 3. Effect of substrate concentration on partially purified enzymes. The assay was performed for PPO activity with 50 mM catechol as substrate at pH 6.0 while keeping the temperature for reaction at 30 ◦ C. For TH activity, 30 mM L-tyrosine was the substrate and assay done at pH 7.0 and 25 ◦ C.

Fig. 2 in L-DOPA synthesis in Mucuna pruriens (L.) DC. is regulated by polyphenol oxidase and not CYP 450/tyrosine hydroxylase: An analysis of metabolic pathway using biochemical and molecular markers

Fig. 2. Effect of pH on the activity of partially purified enzymes from Mucuna pruriens. The assay was performed using 50 mM catechol and 30 mM L-tyrosine as substrates for the PPO and TH enzyme activity, respectively. Four different buffers with their optimal buffering capacity in the pH range of 3–10 were used in separate assays.

Fig. 7 in L-DOPA synthesis in Mucuna pruriens (L.) DC. is regulated by polyphenol oxidase and not CYP 450/tyrosine hydroxylase: An analysis of metabolic pathway using biochemical and molecular markers

Fig. 7. Homology modelling and secondary structure prediction of PPO enzyme from Mucuna pruriens (a) Predicted secondary structure of PPO (b) Phyre2 protein model for PPO with 3D model dimensions (in Å) (X:49.941 Y:64.463 Z:57.979). Image colored by rainbow N → C terminus (c) Three dimensional SWISS protein model for PPO enzyme with two active copper binding ligands (copper ions bridging oxygen moiety is illustrated as small yellow spheres highlighted in the box), conserved histidine residues and metal complex interactions (in dotted lines). Chain A for Ligand 1: H.183, H.204, H.213, F.367, H.371; metal interactions: A:H.183, A:H.204, A:H.213. Chain A for Ligand 2: H.337, H.341, F.367, H.370, H.371; metal interactions: A:H.337, A:H.341, A:H.371). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 6. The 1800 in L-DOPA synthesis in Mucuna pruriens (L.) DC. is regulated by polyphenol oxidase and not CYP 450/tyrosine hydroxylase: An analysis of metabolic pathway using biochemical and molecular markers

Fig. 6. The 1800 bp amplicon of full-length PPO cDNA obtained after deducing the 5′and 3′ ends through RACE analysis. Lane 1- 1 Kb DNA marker, Lane 2 and 3 the amplicon in duplicate after amplification using gene specific primers.

closedIPD-NOFeb 2026View details →

ClinicalTrials.gov32/100

The Role of CYP8B1 Polymorphisms in Modulating the Biochemical Pathways Affected by SGLT2 Inhibitors in T2DM and Obesity

ClinicalTrials.gov study NCT07120828. IPD Sharing: NO. Countries: 1. Publications: 8.

dryad32/100

Data from: Evolutionary routes to biochemical innovation revealed by integrative analysis of a plant-defense related specialized metabolic pathway

Open the record for dataset details and reuse information.

publicSep 2017View details →

dryad28/100

Berberine protects against sepsis-related acute lung injury in rats via the upregulation of the PPAR - Υ signaling pathway, with amelioration at the cellular level - A functional, immune-histological and biochemical study

Aim: To assess the therapeutic effects of berberine in experimentally induced lung sepsis. Examining its effects on selected cytokines, gene and protein expression and the histopathological findings. Results: Berberine significantly reduced the wet/dry lung ratio, the BALF protein, cell, and neutrophils percentage & the BALF cytokines levels. In addition, pretreatment with berberine decreases the myeloperoxidase and MDA levels and decreases gene expression of NFKB, MCP -1 and the ICAM - 1 by RT - PCR analysis. Thus, suggesting an antioxidant and anti-inflammatory mode of the action. Western blot analysis revealed increased PPAR – Υ expression in the berberine pretreated group compared to the CLP group. Histopathological examination revealed improved histopathological parameters, in the berberine pretreated group, compared to the CLP group. Conclusion: Berberine improves the outcome in sepsis via antioxidant and anti-inflammatory effects, modulation of cytokine levels, upregulation of PPAR - Υ protein expression, and histopathological amelioration.

opencc-zeroNov 2021View details →

zenodo28/100

Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments

Data underlying the figures in the publication “Combinatorial Strategy for Studying Biochemical Pathways in Double Emulsion Templated Cell-Sized Compartments”, published in Adv. Mater., 2020, 32, 2004804. <a href="https://onlinelibrary.wiley.com/doi/full/10.1002/adma.202004804">https://onlinelibrary.wiley.com/doi/full/10.1002/adma.202004804</a> Table of contents: 1. Figure 2; Zip file containing the data for Figure 2. It contains 5 files with the corresponding data:      1.1 Figure 2a-b: Microscopy images and a video of the emulsions flowing in the main channel of the microfluidic chip, and data for Figure 2b containing the numerical data for the histograms and their corresponding regressed normal distributions out of 2361 measured droplets.      1.2 Figure 2c: Dataset for Figure 2c, containing the numerical data for the inner and outer diameter variation as a function of the outer phase flow rate.      1.3 Figure 2d: Dataset for Figure 2d, containing the numerical data for the inner and outer diameter variation as a function of the flow rate ratio.      1.4 Figure 2f: Dataset for Figure 2f, containing the numerical data for the histogram and its regressed normal distribution and the corresponding image.      1.5 Figure 2g-h-i: Dataset for Figure 2g, 2h and 2i, containing the CLSM image of GUVs loaded with carboxyfluorescein at the beginning of the permeability experiment and the numerical values for the fluorescence studies (Figures 2h and 2i). 2. Figure 4; Zip file containing the data for Figure 4h-q. It contains 10 files with the numerical data for the fluorescence study graphs showing the change in intensity over time of fluorescein (Figures 4h-l) and resorufin (Figures 4m-q).

opencc-by-4.0Jul 2021View details →

zenodo28/100

Fig. 1 in L-DOPA synthesis in Mucuna pruriens (L.) DC. is regulated by polyphenol oxidase and not CYP 450/tyrosine hydroxylase: An analysis of metabolic pathway using biochemical and molecular markers

Fig. 1. The proposed hypothetical pathway for catecholamine biosynthesis in Mucuna pruriens.