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758 results for “Candidae”
Candida Pseudoglaebosa Ecology in Sarracenia Purpurea Pitcher Plants at Harvard Forest since 2021
Fungi and bacteria are common members of the microbiomes of carnivorous plants. In the pitcher plant Sarracenia purpurea these microorganisms enter carnivorous pitchers shortly after pitchers develop, and may be relevant for pitcher functioning. We are sampling pitchers repeatedly over several years to better understand the culturable diversity in this habitat, with a focus on the common pitcher yeast Candida pseudoglaebosa and fungi that have the potential to interact with it. C. pseudoglaebosa dominates pitcher plants by arriving early in pitchers, and we are interested in how this yeast’s populations change over time and in response to other pitcher microorganisms. Between 2021 and 2023, we collected pitcher water from Tom Swamp in Harvard Forest and cultured 118 yeast and bacteria colonies from this water. We have found C. pseudoglaebosa and some other yeasts (Papiliotrema, Rhodotorula, and Sporidiobolus); microbial identification is ongoing. We are planning to investigate changes in C. pseudoglaebosa genetic diversity and changes in C. pseudoglaebosa interactions with other pitcher microorganisms over time.
Changes in soil moisture and temperature modify the toxicity of sodium selenite and sodium selenate for Folsomia candida (Collembola) Willem 1902
<p>Effects of sublethal concentrations of selenite and selenate were tested on parameters of mortality, reproduction, growth, and oxidative stress parameters of <em>Folsomia candida</em> (Collembola) in case of different climate scenarios. The standard 20°C and the increased 25°C temperatures were combined with three different soil moisture conditions: drought, standard water content and increased water content.</p>
Effects of life stage on the sensitivity of Folsomia candida to four pesticides
<p>This submission provides R-code and data files for our peer-reviewed work.</p><p>The R-notebook "Analysis_likelihood_ratio_test" contains the code used to estimate the parameters of concentration-response curves (EC10, EC50, LC10, LC50, and slopes) and perform likelihood ratio tests to compare curves from different tested life stages.</p><p>The R-notebook "Figures_Concentration_response_curves" showcases the code used to generate the figures presented in the manuscript.</p><p>The dataset files are provided in CSV format with Comma Separated Values:</p><ul><li>Cyproconazole_FolsomiaCandida_10days_20days_RawData_New.csv</li><li>Imidacloprid_FolsomiaCandida_10days_20days_RawData_New.csv</li><li>Teflubenzuron_FolsomiaCandida_10days_20days_RawData_New.csv</li><li>Thiacloprid_FolsomiaCandida_10days_20days_RawData_New.csv</li></ul><p>The submission includes the following:</p><ul><li>R files: R notebooks described above.</li><li>CSV files: Count data of springtail juveniles and adults.</li></ul><p> </p><p>This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 859891.</p><p>This publication reflects only the authors' view and the European Commission is not responsible for any use that may be made of the information it contains.</p>
Photochromic fluorophores enable imaging of endogenous fusion constructs in Candida albicans
<p>10. Gcn5_Stat : Main dataset for figure 2 stationary phase of the manuscript.</p> <p>5., 6. and 7.Gcn5_Stat are additional datasets for figure 2 stationary phase.</p> <p>11. SC5314_Stat : main negative control dataset for figure 2 depicted in supplementary figure 1.</p> <p>14. Gcn5_Exp : Main dataset for figure 2 exponential phase of the manuscript.</p> <p>12., 13. Gcn5_Exp are additional datasets for figure 2 exponential phase.</p> <p>15. SC5314_Exp : main negative control dataset for figure 2 depicted in supplementary figure 1.</p> <p>___________________________________________________________________________________________________</p> <p>2. Erg11 and 7. Erg11 are main datasets for figure 3 of the manuscript.</p> <p>15. SC5314 (Erg11 control) : negative control dataset for figure 3 depicted in supplementary figure 3.</p>
Evaluación de la Producción de Xilitol mediante Modelos Genómicos en Candida tropicalis
<p>La reducción biotecnológica de xilosa a xilitol utilizando levaduras del género Candida sp., apoyada por técnicas de ingeniería metabólica y modelos metabólicos a escala genómica, mejora la producción de xilitol, ofreciendo un proceso más eficiente, rentable y beneficioso para el medio ambiente en las industrias alimentaria y sanitaria.</p>
SBF-SEM datasets related to the manuscript "Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage" by Lachat et al, 2022.
<p>11 serial block face- scanning electron microscopy (SBF-SEM) datasets described in the manuscript "Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage" by Lachat et al, 2022.</p> <p>Resolution: 10 nm x,y, 100 nm Z.</p> <p>Datasets description and quantification can be found in the Supplementary information.</p>
Data from: Photoinactivation of Yeast and Biofilm Communities of Candida albicans Mediated by ZnTnHex-2-PyP4+ Porphyrin
<p>Data supporting the figures presented in the research article Photoinactivation of Yeast and Biofilm Communities of <em>Candida albicans</em> Mediated by ZnTnHex-2-PyP<sup>4+</sup> Porphyrin.</p> <p> </p> <p><em>Candida albicans</em> is the main cause of superficial candidiasis. While the antifungals available are defied by biofilm formation and resistance emergence, antimicrobial photodynamic inactivation (aPDI) arises as an alternative antifungal therapy. The tetracationic metalloporphyrin Zn(II) <em>meso</em>-tetrakis(<em>N</em>-n-hexylpyridinium-2-yl)porphyrin (ZnTnHex-2-PyP<sup>4+</sup>) has high photoefficiency and improved cellular interactions. We investigated the ZnTnHex-2-PyP<sup>4+</sup> as a photosensitizer (PS) to photoinactivate yeasts and biofilms of <em>C. albicans</em> strains (ATCC 10231 and ATCC 90028) using a blue light-emitting diode. The photoinactivation of yeasts was evaluated by quantifying the colony forming units. The aPDI of ATCC 90028 biofilms was assessed by the MTT assays, propidium iodide (PI) labeling, and scanning electron microscopy. Mammalian cytotoxicity was investigated in Vero cells using MTT assay. The aPDI (4.3 J/cm<sup>2</sup>) promoted eradication of yeasts at 0.8 and 1.5 µM of PS for ATCC 10231 and ATCC 90028, respectively. At 0.8 µM and same light dose, aPDI-treated biofilms showed intense PI labeling, about 89% decrease in the cell viability, and structural alterations with reduced hyphae. No considerable toxicity was observed in mammalian cells. Our results introduce the ZnTnHex-2-PyP<sup>4+</sup> as a promising PS to photoinactivate both yeasts and biofilms of <em>C. albicans</em>, stimulating studies with other <em>Candida </em>species and resistant isolates.</p>
Antifungal activity of propolis extract against Candida albicans in patients with vulvovaginal candidiasis
<p>Abstract</p> <p><strong>Objective:</strong> To evaluate the antifungal activity of propolis extract against <em>Candida Albicans</em> (or <em>C. Albicans</em>) in patients with vulvovaginal candidiasis.</p> <p><strong>Method:</strong> The research presents a quantitative approach with an experimental design, with a population of <em>C. Albicans</em> strains isolated from patients diagnosed with vulvovaginal candidiasis who were admitted to the Gynecology Service of the Maria del Socorro Clinic, district of Ate - Lima, from which a sample of 34 strains was chosen in total, considering three repetitions per strain, obtaining 102 experimental units.</p> <p>The antifungal susceptibility test was carried out by the disc diffusion plate method using a single concentration of propolis extract, with fluconazole as a positive control.</p> <p><strong>Resultados:</strong> Propolis extract was shown to inhibit all strains of <em>C. albicans</em>, as was fluconazole, with the effect of the latter being significantly greater than that of the product under investigation.</p> <p><strong>Conclusion:</strong> The conclusion is that propolis extract does show antifungal activity against <em>C. albicans</em> in patients with vulvovaginal candidiasis.</p> <p><strong>Keywords:</strong> Propolis extract, <em>Candida Albicans</em>, Antifungal activity, Vulvovaginal candidiasis.</p> <p> </p>
Fig. 1. A in Agricultural Trichothecene Mycotoxin Contamination Affects The Life-History And Reduced Glutathione Content Of Folsomia Candida Willem (Collembola)
Fig. 1. A relative growth of Folsomia candida on the relation of the contaminated food; significant difference in mean B. Total reproduction of Folsomia candida in the contaminated
Linked collectors and determiners for: Colectas de la levadura emergente Candida auris en aguas marinas de Bocagrande-Cartagena.
Natural history specimen data linked to collectors and determiners held within, "Colectas de la levadura emergente Candida auris en aguas marinas de Bocagrande-Cartagena". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="https://bionomia.net/dataset/0f74c559-440e-4799-a58f-58222c9419a8">https://bionomia.net/dataset/0f74c559-440e-4799-a58f-58222c9419a8</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/0f74c559-440e-4799-a58f-58222c9419a8">https://gbif.org/dataset/0f74c559-440e-4799-a58f-58222c9419a8</a>. Formatted as a Frictionless Data package.
Data from: A novel strategy based on Zn(II) porphyrins and silver nanoparticles to photoinactivate Candida albicans
<p>Data supporting the figures presented in the research article "A novel strategy based on Zn(II) porphyrins and silver nanoparticles to photoinactivate <em>Candida albicans"</em></p> <p><strong>Background:</strong> Photodynamic inactivation (PDI) has been an attractive alternative to treat <em>Candida albicans</em> infections, especially considering the spread of resistant strains. The combination of the photophysical advantages of Zn(II) porphyrins (ZnPs) and the plasmonic effect of silver nanoparticles (AgNPs) has the potential to further improve PDI. In this context, this study aimed to evaluate PDI action in <em>C. albicans</em> using ZnTnHex-2-PyP<sup>4+</sup> or ZnTE-2-PyP<sup>4+</sup> associated with AgNPs. <strong>Methods:</strong> AgNPs stabilized with polyvinylpyrrolidone (PVP) were chosen to allow for (i) overlap between NP extinction and ZnP absorption spectra and (ii) favor AgNPs-ZnPs contact; prerequisites for exploring the plasmonic effect. Optical and zeta potential (ζ) characterizations were performed, and ROS generation was also evaluated. Yeasts were incubated with ZnPs alone or in combination with AgNPs (AgNPs-ZnPs systems), at various concentrations of ZnPs and two proportions of AgNPs, then irradiated with a blue LED. Interactions between yeasts and the systems (ZnP alone or AgNPs-ZnPs) were evaluated by fluorescence microscopy. <strong>Results:</strong> Subtle spectroscopic changes were observed for ZnPs after association with AgNPs, and the ζ analyses confirmed AgNPs-ZnPs interaction. PDI using ZnP-hexyl (0.8 µM) and ZnP-ethyl (5.0 µM) promoted a 3 and 2 log<sub>10</sub> reduction of yeasts, respectively. On the other hand, AgNPs-ZnP-hexyl (0.2 µM) and AgNPs-ZnP-ethyl (0.6 µM) systems led to complete fungal eradication under the same parameters and lower porphyrin concentrations. An increase in the ROS level was observed when ZnPs were associated with AgNPs. Enhanced interaction of yeasts with AgNPs-ZnPs was observed, when compared with ZnPs alone. <strong>Conclusion:</strong> We hypothesize that the plasmonic effect combined with the greater interactions between cells and AgNPs-ZnPs systems resulted in an efficient and improved <em>C. albicans</em> yeast PDI, encouraging further developments toward inactivation of resistant <em>Candida</em> spp.</p> <p> </p>
Additive and dose-dependent mixture effects of Flumite 200 (flufenzin, acaricide) and Quadris (azoxystrobin, fungicide) on the reproduction and survival of Folsomia candida (Collembola)
<p> Our model organism was Folsomia candida (Collembola). We aimed to gain information on the toxicity of Quadris (azoxystrobin) and Flumite 200 (flufenzine aka. diflovidazine) on survival and reproduction and whether the animals can mitigate the toxicity with soil and/or food avoidance behaviour. Also, we aimed to test the effect of the mixture of these two pesticides. We used the OECD 232 reproduction test, a soil avoidance test, and a food choice test for both single pesticides and their mixture. We prepared the mixtures based on the concentration addition model, so the 50% effective concentrations (EC50) of the single materials were used as one toxic unit with a constant ratio of the two materials in the mixture. In the end, the measured mixture EC and LC (lethal concentration) values were compared to the estimate of the concentration addition model.</p>
Single-molecule Fluorescent In Situ Hybridization (smFISH) for RNA detection in the fungal pathogen Candida albicans dataset
<p><strong>This dataset is connected to the protocol article titled:</strong></p> <p>Single-molecule Fluorescent <em>In Situ</em> Hybridization (smFISH) for RNA detection in the fungal pathogen <em>Candida albicans</em></p> <p><strong>Abstract:</strong></p> <p><em>Candida albicans</em> is the most prevalent human fungal pathogen. Its pathogenicity is linked to the ability of <em>C. albicans</em> to reversibly change morphology and to grow as yeast, pseudohyphal or hyphal cells in response to environmental stimuli. Understanding the molecular regulation controlling those morphological switches remains a challenge that, if solved, could help fight <em>C. albicans</em> infections.</p> <p>While numerous studies investigated gene expression changes occurring during <em>C. albicans</em> morphological switches using bulk approaches (e.g., RNA sequencing), here we describe a single-cell and single-molecule RNA imaging and analysis protocol to measure absolute mRNA counts in morphologically intact cells. To detect endogenous mRNAs in single fixed cells, we optimized a single molecule fluorescent <em>in situ</em> hybridization (smFISH) protocol for <em>C. albicans</em>, which allows one to quantify the differential expression of mRNAs in yeast, pseudohyphae or hyphal cells. We quantified the expression of two mRNAs, cell cycle-controlled mRNA (<em>CLB2)</em> and a transcription regulator (<em>EFG1</em>), which show differential expression in the different morphological cell types and in different nutrient conditions. In this protocol we described in detail the major steps of this approach: growth and fixation, hybridization, imaging, cell-segmentation and mRNA spot analysis. Raw data is provided with the protocol to favour reproducibility. This approach could benefit the molecular characterization of <em>C. albicans</em> and other filamentous fungi, pathogenic or non-pathogenic.</p> <p><strong>Data description:</strong></p> <p>This dataset consists of a FISH experiment spanning two different mRNAs, EFG1 and CLB2, and two different nutrient condition, being SPIDER37 and TSB37 in Candida albicans. For culturing, the C. albicans wildtype strain SC5314 was inoculated at 30 degrees overnight (~15 hours) in 10 mL of TSB medium in a 30 degree (celsius) shaking incubator. Next, samples were diluted to a density of 10^5 cells/ mL and inoculated for 6 hours in either 30 mL TSB medium or Spider medium at 37 degrees in falcon tubes on an orbital microplate shaker. Then, samples were fixated by adding PFA to a final concentration of 4% to the medium. For hybridization, both mRNAs were hybridized independently by specific DNA oligo labelled with a Quasar670 dye to enable the visualisation of single mRNA molecules. As both genes are labelled by the same dye, these oligos were not co-applied to the same sample but to independent samples.</p> <p><strong>Microscopy</strong></p> <p>For smFISH imaging we use an Olympus BX-63 epifluorescence microscope equipped with Ultrasonic stage and UPlanApo 100x 1.35NA oil-immersion objective (Olympus). Lumencore SOLA FISH light source, a Hamamatsu ORCA-Fusion sCMOS camera (6.5 µm-pixel size) mounted using U-CMT C-Mount Adapter, and zero-pixel shift filter sets: F36-500 DAPI HC Brightline Bandpass Filter, F36-502 FITC HC BrightLine Filter, F36-542 Cy3 HC BrightLine Filter, and F36-523 Cy5 HC BrightLine Filter. Images are acquired across 61-81 optical sections (depending on the sample thickness) with a z-step size of 0.2 μm. The CellSens software (Olympus) is used for instrument control and image acquisition. For the DAPI channel 10-50 ms of exposure was used. Whilst, for the CY5 channel, used imaging the FISH probes, 750 ms was applied. </p> <p> </p>
Single-molecule Fluorescent In Situ Hybridization (smFISH) for RNA detection in the fungal pathogen Candida albicans small example dataset
<p><strong>This small example dataset is connected to the protocol article titled:</strong></p> <p>Single-molecule Fluorescent <em>In Situ</em> Hybridization (smFISH) for RNA detection in the fungal pathogen <em>Candida albicans</em></p> <p><strong>Abstract:</strong></p> <p><em>Candida albicans</em> is the most prevalent human fungal pathogen. Its pathogenicity is linked to the ability of <em>C. albicans</em> to reversibly change morphology and to grow as yeast, pseudohyphal or hyphal cells in response to environmental stimuli. Understanding the molecular regulation controlling those morphological switches remains a challenge that, if solved, could help fight <em>C. albicans</em> infections.</p> <p>While numerous studies investigated gene expression changes occurring during <em>C. albicans</em> morphological switches using bulk approaches (e.g., RNA sequencing), here we describe a single-cell and single-molecule RNA imaging and analysis protocol to measure absolute mRNA counts in morphologically intact cells. To detect endogenous mRNAs in single fixed cells, we optimized a single molecule fluorescent <em>in situ</em> hybridization (smFISH) protocol for <em>C. albicans</em>, which allows one to quantify the differential expression of mRNAs in yeast, pseudohyphae or hyphal cells. We quantified the expression of two mRNAs, cell cycle-controlled mRNA (<em>CLB2)</em> and a transcription regulator (<em>EFG1</em>), which show differential expression in the different morphological cell types and in different nutrient conditions. In this protocol, we described in detail the major steps of this approach: growth and fixation, hybridization, imaging, cell-segmentation and mRNA spot analysis. Raw data is provided with the protocol to favour reproducibility. This approach could benefit the molecular characterization of <em>C. albicans</em> and other filamentous fungi, pathogenic or non-pathogenic.</p> <p><strong>Data description:</strong></p> <p>This dataset consists of a FISH experiment spanning two different mRNAs, EFG1 and CLB2, and one nutrient condition, SPIDER37, in Candida albicans. For culturing, the C. albicans wildtype strain SC5314 was inoculated at 30 degrees overnight (~15 hours) in 10 mL of TSB medium in a 30 °C shaking incubator. Next, samples were diluted to a density of 10^5 cells/ mL and inoculated for 6 hours in 30 mL Spider medium at 37 °C in falcon tubes on an orbital microplate shaker. Then, samples were fixated by adding PFA to a final concentration of 4% to the medium. For hybridization, both mRNAs were hybridized independently by specific DNA oligo labelled with a Quasar670 dye to enable the visualisation of single mRNA molecules. As both genes are labelled by the same dye, these oligos were not co-applied to the same sample but to independent samples.</p> <p><strong>Microscopy</strong></p> <p>For smFISH imaging we use an Olympus BX-63 epifluorescence microscope equipped with Ultrasonic stage and UPlanApo 100x 1.35NA oil-immersion objective (Olympus). Lumencore SOLA FISH light source, a Hamamatsu ORCA-Fusion sCMOS camera (6.5 µm-pixel size) mounted using U-CMT C-Mount Adapter, and zero-pixel shift filter sets: F36-500 DAPI HC Brightline Bandpass Filter, F36-502 FITC HC BrightLine Filter, F36-542 Cy3 HC BrightLine Filter, and F36-523 Cy5 HC BrightLine Filter. Images are acquired across 61-81 optical sections (depending on the sample thickness) with a z-step size of 0.2 μm. The CellSens software (Olympus) is used for instrument control and image acquisition. For the DAPI channel 10-50 ms of exposure was used. Whilst, for the CY5 channel, used for imaging the FISH probes, 750 ms was applied. </p>
Nymphaea candida J.Presl & C.Presl (BR0000010339329)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Nymphaea candida J.Presl & C.Presl (BR0000010338575)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Nymphaea candida J.Presl & C.Presl (BR0000012438020)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Nymphaea candida J.Presl & C.Presl (BR0000012438129)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Nymphaea candida J.Presl & C.Presl (BR0000012635405)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
Nymphaea candida J.Presl & C.Presl (BR0000012438228)
Belgium Herbarium image of <a href="https://www.plantentuinmeise.be">Meise Botanic Garden</a>.
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OpenNeuro
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