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141 results for “Cell tracking”

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zenodo44/100

Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs

<p>Supplementary data and videos for the manuscript 'Long-term live imaging, cell identification and cell tracking in regenerating crustacean legs', by &Ccedil;evrim,<sup> </sup>Laplace-Builh&eacute;,<sup> </sup>Sugawara, Rusciano, Labert, Brocard, Almaz&aacute;n and Averof.</p> <p>The supplementary data include:</p> <p><strong>Supplementary Data 1 (.csv file);&nbsp; Live imaging of regenerating <em>Parhyale</em> legs: image acquisition settings</strong></p> <p>Table with information on the 22 time lapse recordings presented in Figure 3, including image acquisition settings, temperature and duration of the recordings.</p> <p><strong>Supplementary Data 2 (.zip file);&nbsp; Live imaging of regenerated <em>Parhyale</em> legs: maximum projections</strong></p> <p>Compressed folder including maximum projections for each of the 22 time lapse recordings presented in Figure 3. These files were generated by projecting all or a subset of the z slices acquired at each time point. A 20 micron scale bar was added on the first time point. These files serve as a quick way to examine the 22 time lapse recordings.</p> <p><strong>Supplementary Data 3 (22 .tif files);&nbsp; Live imaging of regenerated <em>Parhyale</em> legs: complete datasets</strong></p> <p>Complete image 3D+T hyperstacks for each of the 22 time lapse recordings presented in Figure 3. These files have been generated by concatenating the original image stacks and correcting any image shifts, as described in the Methods section of the paper.</p> <p><strong>Supplementary Data 4 (.zip file);&nbsp; Analysis of trade-offs of imaging resolution and image quality</strong></p> <p>The data used for the analysis of trade-offs in imaging and the results shown in Table 1 are included in this compressed folder. Folders for the original recording (labelled 00), for each of the subsampled datasets (labelled 01 to 05), and for the denoised and deconvoluted datasets each include the corresponding image data and ground truth cell tracking files (.tif, .h5, .xml and .mastodon files) and three sets of cell track predictions (.mastodon files). There are also separate folders containing the Elephant detection and flow model parameters for each set of predictions.</p> <p dir="ltr"><strong>Supplementary Data 4 (.zip file);&nbsp; Analysis of trade-offs of imaging resolution and image quality</strong></p> <p dir="ltr">The data used for the analysis of trade-offs in imaging and the results shown in Table 1 are included in two folders. The folder named Image_and_tracking_data includes the image data (.tif, .h5, .xml), ground truth cell tracking files (.mastodon files) and three sets of cell track predictions (.mastodon files) for the original recording (labelled 00), for each of the subsampled datasets (labelled 01 to 05), and for the denoised and deconvoluted datasets. It also includes separate folders containing the Elephant detection and flow model parameters for each set of predictions. The folder named CTC_tracking_results includes the ground-truth data along with three sets of predictions for detection and tracking for each dataset, following the Cell Tracking Challenge format. For each dataset we include label image files (.tif) for every time point along with tracking results in .txt format, and each results directory (01_RES_*) also contains the evaluation results from the Cell Tracking Challenge Evaluation Software. For a detailed explanation of the folder structure, please refer to the Cell Tracking Challenge documentation.</p> <p><strong>Supplementary Data 5 (.zip file);&nbsp; Tracking the progenitors of spineless-expressing cells in the distal carpus</strong></p> <p>The data used to generate Figure 7 are included in this compressed folder, including the live imaging and cell tracking files (.h5, .xml and .mastodon files) and the image stack of the spineless and futsch HCR and DAPI stainings (.tif file). Channel 2 shows spineless expression (mostly nascent transcripts in nuclei), as well as background signal in epidermal nuclei (possibly due to photoconversion of DAPI, see Karg &amp; Golic 2018, Chromosoma 127: 235-245) and strong autofluorescence in granular cells (also visible in channel 1, depicting futsch HCR).</p> <p><strong>Supplementary Data 6 (.txt file);&nbsp; Sequences of <em>Parhyale</em> genes targeted by the HCR probes</strong></p> <p>The sequences are provided in FASTA format.</p> <p dir="ltr"><strong>Supplementary Data 7 (.zip file);&nbsp; Apoptosis in legs that have not been subjected to live imaging</strong></p> <p dir="ltr">The data used to generate Figure 2 supplement 2 are contained in this compressed folder, including 9 image stacks of T4 and T5 legs fixed and stained with DAPI 3 days post amputation (with apoptotic nuclei marked) and a .txt file containing the apoptotic cell counts.</p> <p dir="ltr"><strong>Supplementary Data 8 (.zip file);&nbsp; Analysis of tracking performance in relation to imaging depth</strong></p> <p dir="ltr">The data used to generate Figure 5 are contained in this compressed folder, including separate folders for the data extracted from the analysis of datasets #1 to #5. Each folder includes data from three replicates (batches 001 to 003), with .csv files listing the z location of nucleus centroids (in &micro;m) for the nuclei that were incorrectly detected by Elephant &ndash; either as false positives (FP) or as false negatives (FN) &ndash; and the ground truth data (GT). The folder also includes an .xlsx file gathering all the relevant data and the measurements of precision and recall.</p> <p dir="ltr"><strong>Supplementary Data 9 (.zip file);&nbsp; Detecting the temporal pattern of cell divisions in regenerating legs</strong></p> <p dir="ltr">The data used to generate Figure 4 are contained in this compressed folder, including the five image datasets (.tif, .h5, .xml), the detected cell divisions (.mastodon files), and an .xlxs file containing all the cell divisions counts and graphs.</p> <p><strong>Video 1.&nbsp; Time lapse recording of regeneration in a Parhyale T5 leg (dataset li48-t5)</strong></p> <p>Live imaging of nuclei labelled with H2B-mREFruby (maximum projection of z slices 3-10). Proximal parts of the leg are to the left and the amputation site is at the right of the frame. For annotations of different features please refer to Figure 2. Shortly after leg amputation (0 hpa) hemocytes adhere to the wound. By 16 hpa the wound has melanized. Up to ~32 hpa epithelial cells can be seen migrating and accumulating at the wound, below the melanized scab (Figure 2A,B). Around 31 hpa, the leg tissues become detached from the scab (Figure 2C). At 43 hpa, the carpus-propodus boundary first becomes visible, and thereafter many cells can be observed dividing at the distal part of the leg stump (Figure 2D). At 56 hpa, the propodus-dactylus boundary first becomes visible (Figure 2E). At later stages, tissues in more proximal parts of the leg retract, making space for the regenerating leg to grow (Figure 2F,G). After ~90 hpa cell proliferation there is less cell proliferation and cell movements, and the nuclear positions within the tissue become fixed. Scale bars, 20 &micro;m.</p> <p><strong>Video 2.&nbsp; Time lapse recording of regeneration in a Parhyale T5 leg (dataset li36-t5)</strong></p> <p>Live imaging of nuclei labelled with H2B-mREFruby (maximum projection of z slices 3-15). Proximal parts of the leg are to the left and the amputation site is at the right of the frame. The sequence of events is similar to that described in Video 1, but the progression is slower: epithelial migration towards the wound is observed up to 40 hpa, tissues detach from the scab at 65 hpa, and the carpus-propodus and propodus-dactylus boundaries first become visible at 78 and 91 hpa. The tissues making up the carpus and propodus can be seen pulsating from 105 to 145 hpa. Scale bars, 20 &micro;m.</p>

opencc-by-4.0Sep 2024View details →
zenodo44/100

Data: multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes

<p>This data set includes multispectral optoacoustic tomography images supporting an article on cell tracking (preprint: bioRxiv 199836; https://doi.org/10.1101/199836). The corresponding bioluminescence results are included too, as well as the spectra used for the multispectral processing. </p>

opencc-by-4.0Oct 2017View details →
zenodo44/100

Dataset for "Blue-shift photoconversion of near-infrared fluorescent proteins for labeling and tracking in living cells and organisms"

<p>Dataset that supports the observation, characterization and application of the blue-shift photoconversion of the near infrared proteins, miRFPs, reported in the manuscript: &quot;Blue-shift photoconversion of near-infrared fluorescent proteins for labeling and tracking in living cells and organisms&quot;. The data references to the specific figures and&nbsp;graphs in the manuscript.</p>

opencc-by-4.0Mar 2023View details →
zenodo40/100

Immune repertoire profiling reveals that clonally expanded B and T cells infiltrating diseased human kidneys can also be tracked in the blood

<p>Recent advances in high-throughput sequencing allow for the competitive analysis of the human B and T cell immune repertoire. In this study we compared Immunoglobulin and T cell receptor repertoires of lymphocytes found in kidney and blood samples of 10 patients with various renal diseases based on next-generation sequencing data.</p>

opencc-by-sa-4.0Aug 2015View details →
zenodo40/100

ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy

<p>Data for submission to Wellcome Open Research entitled "ultraLM and miniLM: Locator tools for smart tracking of fluorescent cells in correlative light and electron microscopy".</p> <p>Data_ultraLM.tif is an image stack from the fluorescence microscope mounted on the ultramicrotome.</p> <p>Data_miniLM.tif is an image stack from the fluorescence microscope mounted in the SBF-SEM.</p> <p>Data_miniLM_EM.tif is an image stack from the SBF-SEM while the miniLM was in-situ.</p>

opencc-by-4.0Dec 2016View details →
zenodo40/100

3D cell tracking dataset of bacterial biofilm deformation and recovery under shear flow

<p>This MAT file includes dataset in the scientific article "<i>In vivo</i> microrheology reveals elastic and plastic responses inside three-dimensional bacterial biofilms" by the following authors: Takuya Ohmura, Dominic Skinner, Konstantin Neuhaus, Gary Choi, Jörn Dunkel, Knut Drescher. This MAT file can be conveniently opened with Matlab.&nbsp;</p><p>When you open this file with Matlab, you will find 4 variables stored in the file "Data_v3_loop2_newRxy_bidx1_274.mat"</p><p><strong>Variable 1: name_parameter</strong></p><p>Names of 31 parameters for columns in 3 variables: 'deformation_all', 'recovery_all' , 'plasticity_all'. The parameters have cell displacements, orientations, coordinates, biofilm indexes and experimental conditions. When the parameters have units, they are shown in the names.&nbsp;</p><ul><li>'x_Frame1[um]'</li><li>'y_Frame1[um]'</li><li>'z_Frame1[um]'</li><li>'Normalized_x_Frame1'</li><li>'Normalized_y_Frame1'</li><li>'Normalized_z_Frame1'</li><li>'LocalDensity_Frame1(VolumeFractionAround30px)'</li><li>'LocalCellNumberDensity_Frame1(VolumeFractionAround30px)'</li><li>'NematicOrderParameter_Frame1'</li><li>'AlignmentFlow_Frame1[rad]'</li><li>'AlignmentRadial_Frame1[rad]'</li><li>'AlignmentZaxis_Frame1[rad]'</li><li>'d_x[um]'</li><li>'d_y[um]'</li><li>'d_z[um]'</li><li>'Normalized_d_x'</li><li>'Normalized_d_y'</li><li>'Normalized_d_z'</li><li>'d_LocalDensity'</li><li>'d_LocalNumberDensity[um^-3]'</li><li>'d_NematicOrderParameter'</li><li>'d_AlignmentFlow[rad]'</li><li>'d_AlignmentRadial[rad]'</li><li>'d_AlignmentZaxis[rad]'</li><li>'CrossProduct[um^2]'</li><li>'BiofilmIndexNumber'</li><li>'Biofilm_width[um]'</li><li>'Biofilm_height[um]'</li><li>'Biofilm_volume[um^3]'</li><li>'FlowRate[ul/min]'</li><li>'Duration[min]'</li></ul><p><strong>Variable 2:&nbsp;deformation_all</strong></p><p>The rows indicate 704198 single-cell trackings in deformations of 274 bacterial biofilms. Each of the 274 bacterial biofilms has a different 'BiofilmIndexNumber'. The columns indicate 31 parameters which names are shown in 'name_parameter'.</p><p><strong>Variable 3: recovery_all</strong></p><p>The rows indicate 685991 single-cell trackings in recoveries of 274 bacterial biofilms. Each of the 274 bacterial biofilms has a different 'BiofilmIndexNumber'. The columns indicate 31 parameters which names are shown in 'name_parameter'.</p><p><strong>Variable 4: plasticity_all</strong></p><p>The rows indicate 665749 single-cell trackings in plasticities of 274 bacterial biofilms. Each of the 274 bacterial biofilms has a different 'BiofilmIndexNumber'. The columns indicate 31 parameters which names are shown in 'name_parameter'.</p><p>&nbsp;</p><p>To plot the cell tracked data in the figures of the article, use our MATLAB code uploaded in our GitHub (https://github.com/knutdrescher/biofilm-rheology).</p>

opencc-by-4.0Nov 2023View details →
dryad40/100

Ultra-sensitive and multiplexed tracking of single cells using whole-body PET/CT

<p><em>In vivo </em>molecular imaging tools are crucially important for elucidating how cells move through complex biological systems, however, achieving single-cell sensitivity over the entire body remains challenging. Here, we report a highly sensitive and multiplexed approach for tracking upwards of 20 single cells simultaneously in the same subject using positron emission tomography (PET). The method relies on a statistical tracking algorithm (PEPT-EM) to achieve a sensitivity of 4 Bq/cell, and a streamlined workflow to reliably label single cells with over 50 Bq/cell of <sup>18</sup>F-fluorodeoxyglucose (FDG). To demonstrate the potential of the method, we tracked the fate of over 70 melanoma cells after intracardiac injection and found they primarily arrested in the small capillaries of the pulmonary, musculoskeletal, and digestive organ systems. This study bolsters the evolving potential of PET in offering unmatched insights into the earliest phases of cell trafficking in physiological and pathological processes and in cell-based therapies.</p>

opencc-zeroMay 2024View details →
dryad40/100

Data from: A cerebellar granule cell–climbing fiber computation to learn to track long time intervals

<p>In classical cerebellar learning, Purkinje cells (PkCs) associate climbing fiber (CF) error signals with predictive granule cells (GrCs) active just prior (~150ms). Cerebellum also contributes to behaviors characterized by longer timescales. To investigate how GrC-CF-PkC circuits might learn seconds-long predictions, we imaged simultaneous GrC-CF activity over days of forelimb operant conditioning for delayed water reward. As mice learned reward timing, numerous GrCs developed anticipatory activity ramping at different rates until reward delivery, followed by widespread time-locked CF spiking. Relearning longer delays further lengthened GrC activations. We computed CF-dependent GrC→PkC plasticity rules, demonstrating that reward-evoked CF spikes sufficed to grade many GrC synapses by anticipatory timing. We predicted and confirmed that PkCs could thereby continuously ramp across seconds-long intervals from movement to reward. Learning thus leads to new GrC temporal bases linking predictors to remote CF reward signals—a strategy well-suited to learn to track long intervals common in cognitive domains.</p>

opencc-zeroJun 2024View details →
zenodo40/100

Tracking cells in Xenopus tissue with TrackMate-MorphoLibJ

<p>Tracking cells in Xenopus tissue with TrackMate-MorphoLibJ.</p> <p>For more details see&nbsp;https://imagej.net/plugins/trackmate/trackmate-morpholibj</p> <p>Image courtesy of Jakub Sedzinski.</p> <p>&nbsp;</p> <p>&nbsp;</p>

opencc-by-4.0Aug 2021View details →
zenodo40/100

Tracking cell migration with the TrackMate threshold detector

<p>Migrating cells tracked with TrackMate, using the threshold detector.</p> <p>For more information see&nbsp;https://imagej.net/plugins/trackmate/trackmate-thresholding-detector</p> <p>&nbsp;</p>

opencc-by-4.0Aug 2021View details →
zenodo40/100

Tracking Data I/II of the publication "A graph-based cell tracking algorithm with few manually tunable parameters and automated segmentation error correction"

<p>DATA belonging to the paper<br> &quot;A graph-based cell tracking algorithm with few manually tunable parameters and automated segmentation error correction&quot;<br> Katharina L&ouml;ffler, Tim Scherr, Ralf Mikut<br> doi: https://doi.org/10.1101/2021.03.16.435631</p> <p>-----------------------------</p> <p>To investigate the influence of different segmentation errors on the tracking performance we simulate errorneous segmentation data:<br> - under-segmentation (referred to as &quot;merge&quot; in the folders), over-segmentation(&quot;split&quot;), False Negatives (&quot;remove&quot;), combination of the aforementioned errors (&quot;mixed&quot;)<br> - percentages: 1,2,5,10,20 of errorneous masks per dataset<br> - runs: 5 randomly initialized runs per combination<br> - datasets: Fluo-N2DH-SIM+ and Fluo-N3DH-SIM+ each with two image sequences<br> ---&gt; in total 4 (error types) * 5 (percentage) * 5 (runs) * 2 (data sets) * 2 (image sequences) = 400 datasets</p> <p>The datasets can be recreated by running our code https://git.scc.kit.edu/KIT-Sch-GE/2021-cell-tracking<br> ----------------------------</p> <p>RESULTS<br> We evuated the four tracking algorithms KIT-Sch-GE(1), KTH-SE, MU-Lux-CZ and our proposed algorithm on the aforementioned datasets and compare their performance using the CTC metrics DET, SEG and TRA.<br> This repository contains all metrics as xls files and all tracking results as image sequences.</p> <p><br> <strong>xls files</strong><br> -----------<br> compare_all_trackers_on_synt_bm.csv<br> Comparing the tracking algorithms MU-Lux-CZ, KTH-SE, KIT-Sch-GE(1) and the proposed tracking algorithm on synthetically degraded segmentation data Fluo-N2DH-SIM+ and Fluo-N3DH-SIM+ (Cell Tracking Challenge datasets).<br> Reported scores are DET, SEG and TRA from the Cell Tracking Challenge<br> (Fig8 and Fig9 and Supplementary Figures 3 and 4 are created from this data)</p> <p><br> compare_postprocessing_on_synth_bm.csv<br> Comparing the different post-processing strategies of the proposed tracking algorithm algorithm on synthetically degraded segmentation data Fluo-N2DH-SIM+ and Fluo-N3DH-SIM+ (Cell Tracking Challenge datasets).<br> Reported scores are DET, SEG and TRA from the Cell Tracking Challenge<br> (Fig7 and Fig8 and Supplementary Figures 1 and 2 are created from this data)</p> <p><strong>PLEASE NOTE: the folder compare_postprocessing_synth_bm&nbsp; is provided in the repository&nbsp;10.5281/zenodo.5227610 due to size restrictions.</strong></p> <p><strong>folders </strong>(decompressed approximately 90GB of data!)<br> -----------<br> tracking_data<br> &nbsp;&nbsp; &nbsp;compare_all_synth_bm<br> &nbsp;&nbsp; &nbsp;Contains all tracking results for each tracking algorithm on the synthetically degraded datasets ()</p> <p>&nbsp;&nbsp; &nbsp;compare_all_synth_bm_no_error<br> &nbsp;&nbsp; &nbsp;Contains the tracking results for each tracking algorithm provided with the perfect ground truth segmentation data</p> <p>&nbsp;&nbsp; &nbsp;compare_postprocessing_synth_bm [<strong>will be stored in 10.5281/zenodo.5227610 due to size restrictions</strong>]<br> &nbsp;&nbsp; &nbsp;Contains all tracking resuls for each postprocessing configuration of the proposed cell tracking algorithm<br> &nbsp;&nbsp; &nbsp;the leaf folders are names run_xPOSTPROCESSING where x is the run number and POSTPROCESSING the postprocessing key<br> &nbsp;&nbsp; &nbsp;Postprocessing keys: (&quot;no untangle&quot; or &quot;no masks&quot; is indicated by an overline in the paper)<br> &nbsp;&nbsp; &nbsp;all (&quot;untangle + masks&quot; in the paper)<br> &nbsp;&nbsp; &nbsp;nd (&quot;no untangle + masks&quot;)<br> &nbsp;&nbsp; &nbsp;nd_ns-l (&quot;no untangle + no masks&quot;)<br> &nbsp;&nbsp; &nbsp;ns-l (&quot;untangle + no masks&quot;)</p>

opencc-by-4.0Aug 2021View details →
zenodo40/100

Outputs from new methods for 3D+time cell image segmentation and tracking

<p>Segmentation and tracking of 3D+time microscopy images of cell nuclei within the zebrafish pectoral fin.</p> <p>The file named 7_cells_moving_in_70_frames_orig.avi is a 70-frame&nbsp;video&nbsp;of a group of cells moving in time, the file named&nbsp;7_cells_moving_in_70_frames.avi has the result of 4D segmentation,&nbsp;using our new segmentation methods,&nbsp;for seven&nbsp;cells (colored black) moving in time, and the file _tracking_of_7_cell_in_70_frames.mp4 has the tracking of these seven cells.&nbsp;</p> <p>Additionally, the file named group_of_cells_moving_in_70_frames_orig.avi is a 70-frame&nbsp;video&nbsp;of a group of cells moving in time, the file named&nbsp;group_of_cells_moving_in_70_frames.avi has the result of 4D segmentation,&nbsp;using our new segmentation methods,&nbsp;for the group of&nbsp;cells (colored black) moving in time and the file _tracking_of_group_of_cell_in_70_frames.gif has the tracking of this&nbsp;group of&nbsp;cells.&nbsp;</p>

opencc-by-4.0Jul 2021View details →
zenodo40/100

Data for - Tracking one-in-a-million: Large-scale benchmark for microbial single-cell tracking with experiment-aware robustness metrics

<p><strong>Large-scale Corynebacterium glutamicum data set with Segmentation and Tracking Annotation</strong></p> <p>We provide five time-lapse sequences with manually corrected segmentation and tracking annotations of growing&nbsp;<strong><em>C. glutamicum</em></strong>&nbsp;cultivations. The dataset contains more than 1.4 million cell observations in 29k cell tracks and 14k cell divisions. We provide videos of the annotations (videos.zip) and the dataset in&nbsp;<a href="http://celltrackingchallenge.net/datasets/">Cell Tracking Challenge</a>&nbsp;format (ctc_format.zip). In the videos, cell contours are rendered in yellow, cell links between frames are colored red and cell divisions, and their links are colored in blue.</p> <p><strong>Data Acquisition</strong></p> <p><strong><em>Corynebacterium glutamicum</em></strong>&nbsp;ATCC 13032 was cultivated in BHI-medium at 30&deg;C in this study. From and overnight preculture, the main culture was inoculated the next day with a starting OD600 of 0.05 and grown at 120 rpm to a OD600 of 0.25. A chip was fabricated, according to&nbsp;<a href="https://doi.org/10.1039/D0LC00711K">(T&auml;uber et al., 2020)</a>, and fixed to the microscope&rsquo;s holder. The main culture cells were transferred to monolayer growth chambers (height = 720 nm) on the microfluidic chip. Flow through the microfluidic device was mediated by pressure driven pumps with a pressure of 100 mbar on the medium reservoir.</p> <p>The time-lapse phase contrast images of five monolayer growth chambers were taken every minute using an inverted microscope (Nikon Eclipse Ti2) with a 100x oil emersion objective and a DS-QI2 camera (Nikon) at 15 % relative DIA-illumination intensity and 100 ms exposure time. The spatial image resolution is 0.072 &mu;m/px.</p>

opencc-by-4.0Oct 2022View details →
zenodo40/100

Mastodon project containing the cell tracks of a developing Tribolium Castaneum embryo.

<p>Mastodon project containing the cell tracks of a developing <em>Tribolium Castaneum </em>embryo.&nbsp;</p> <p>To open it in Mastodon, download both the <em>.mastodon </em>and the <em>.xml </em>files and put them in the same folder.</p> <p>The image data is stored on a BigDataServer in the Institut Pasteur, Paris, provided by the <a href="https://research.pasteur.fr/en/team/image-analysis-hub/">Image Analysis Hub</a>. When you open this <em>.mastodon </em>file with Mastodon, it will stream the image from the server without you having to download it. The mastodon file contains just the tracks and the <em>.xml </em>file contins the address of the server from where the image data will be streamed.</p> <p>Check the&nbsp;<a href="https://mastodon.readthedocs.io/">Mastodon scientific software</a>&nbsp;for information on how to open and use&nbsp;this file for scientific purposes.</p> <p>The image data is the &#39;TRIF&#39; training video 02 from the <a href="http://www.celltrackingchallenge.net/">CellTrackingChallenge.net</a>, with authorization. The original dataset provider is&nbsp;Dr. A. Jain. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.</p> <ul> <li>Microscope: Zeiss LightSheet LZ.1</li> <li>Objective lens: Plan-Apochromat 20x/1.0 (water)</li> <li>Voxel size (microns): 0.38 x 0.38 x 0.38</li> <li>Time step (min): 1.5</li> </ul>

opencc-by-4.0Nov 2022View details →
zenodo40/100

Live Cells for contour tracking

<p>Pre-processed live cell dataset in tfrecord format.&nbsp;</p> <p>It was used for&nbsp;training&nbsp;and inference of our contour tracking model.</p>

opencc-by-4.0Mar 2023View details →
zenodo40/100

Imaging data from "Live-cell 3D single-molecule tracking reveals modulation of enhancer dynamics by NuRD"

<p>3D 20ms, 3D&nbsp;500ms and 2D dCas9 raw videos, localisation, tracking and trajectory analysis&nbsp;data</p> <p>From&nbsp;&#39;Live-cell 3D single-molecule tracking reveals modulation of enhancer dynamics by NuRD&quot; (2021). Biorxiv. https://doi.org/10.1101/2020.04.03.003178</p>

opencc-by-4.0May 2023View details →
dryad40/100

Ultra-sensitive and multiplexed tracking of single cells using whole-body PET/CT

Open the record for dataset details and reuse information.

publicMay 2024View details →
dryad40/100

Plastid and peroxisome movement tracks in the root cells of Arabidopsis thaliana

Open the record for dataset details and reuse information.

publicNov 2024View details →
dryad40/100

Data from: A cerebellar granule cell–climbing fiber computation to learn to track long time intervals

Open the record for dataset details and reuse information.

publicJun 2024View details →
dryad36/100

Fluorescent (C)LSM image sequences of Dictyostelium discoideum (Ax2 - LifeAct mRFP) for cell track and cell contour analysis

<p>This data set is designed for cell contour and cell track analysis. Hence image sequences of moving Dictyostelium discoideum cells are recorded. For the purpose to facilitate the detection of the cell contour we take fluorescent images of the cortical protein actin to obtain a high contrast between background and cell body. In each image sequences several cells are recorded. This allows for an analysis of all cells at once or to crop single cell tracks.</p>

opencc-zeroSep 2020View details →

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Allen Brain Atlas

Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.

allen-brain-atlas
neuroscienceopenDocumentation, web resources, and API references are available online.
Last verified 2026-04-30Open record

Annotated Behaviour and Observability Dataset (ABODe)

ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.

abode-home-cage
behavioral-neuroscienceopenThe DataShare record exposes download links for annotations, documentation, license text, and the zipped per-snippet data directory.
Last verified 2026-04-30Open record

DANDI Archive for NWB datasets

DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.

dandi-nwb
electrophysiologyopenPublished Dandiset metadata and archive endpoints are available through the production DANDI API.
Last verified 2026-04-30Open record

International Brain Laboratory public data

The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.

ibl
behavioral-neuroscienceopenPublic sessions can be searched and loaded from the IBL public data server through ONE.
Last verified 2026-04-29Open record

OpenNeuro

OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.

openneuro
neuroscienceopenPublished datasets are available on demand over the internet.
Last verified 2026-04-29Open record