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87 results for “Chlamydia trachomatis”
FtsK is critical for the assembly of the unique divisome complex of the FtsZ-less Chlamydia trachomatis: blots
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Supplemental figures for: FtsK is critical for the assembly of the unique divisome complex of the FtsZ-less Chlamydia trachomatis IF images
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Source data: FtsK is critical for the assembly of the unique divisome complex of the FtsZ-less Chlamydia trachomatis figures 1-7
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Source data: FtsK is critical for the assembly of the unique divisome complex of the FtsZ-less Chlamydia trachomatis IF images
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Distinct roles of the Chlamydia trachomatis effectors TarP and TmeA in the regulation of formin and Arp2/3 during entry
<p>The obligate intracellular pathogen Chlamydia trachomatis manipulates the host actin cytoskeleton to assemble actin-rich structures that drive pathogen entry. The recent discovery of TmeA, which, like TarP, is an invasion-associated type III effector implicated in actin remodeling, raised questions regarding the nature of their functional interaction. Quantitative live-cell imaging of actin remodeling at invasion sites revealed differences in recruitment and turnover kinetics associated with the TarP and TmeA pathways, with the former accounting for most of the robust actin dynamics at invasion sites. TarP-mediated recruitment of actin nucleators, i.e. formins and the Arp2/3 complex, was crucial for rapid actin kinetics, generating a collaborative positive feedback loop that enhanced their respective actin-nucleating activities within invasion sites. In contrast, the formin Fmn1 was not recruited to invasion sites and did not collaborate with Arp2/3 within the context of Tme-aassociated actin recruitment. Although the TarP-Fmn1-Arp2/3 signaling axis is responsible for the majority of actin dynamics, its inhibition had similar effects as the deletion of TmeA on invasion efficiency, consistent with the proposed model that TarP and TmeA act on different stages of the same invasion pathway.</p>
Comparing mail-in self-collected specimens sent via United States Postal Service versus clinic-collected specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in extra-genital sites data set
<p>This data set was used to evaluate the concordance between clinic-collected extra-genital specimens and self-collected mailed-in extra-genital specimens among participants seeking sexually transmitted infection testing at a free clinic in Hollywood, CA. The newest version of the file reflects sample adequacy control (SAC) cycle threshold values for each participant.</p>
Dynamin-dependent entry of Chlamydia trachomatis is sequentially regulated by the effectors TarP and TmeA
<p><em>Chlamydia</em> invasion of epithelial cells is a pathogen-driven process involving two functionally distinct effectors – TarP and TmeA. They collaborate to promote robust actin dynamics at sites of entry. Here, we extend studies on the molecular mechanism of invasion by implicating the host GTPase dynamin 2 (Dyn2) in the completion of pathogen uptake. Importantly, Dyn2 function is modulated by TarP and TmeA at the levels of recruitment and activation through oligomerization, respectively. TarP-dependent recruitment requires phosphatidylinositol 3-kinase and the small GTPase Rac1, while TmeA has a post-recruitment role related to Dyn2 oligomerization. This is based on the rescue of invasion duration and efficiency in the absence of TmeA by the Dyn2 oligomer-stabilizing small molecule activator Ryngo 1-23. Notably, Dyn2 also regulated the turnover of TarP- and TmeA-associated actin networks, with disrupted Dyn2 function resulting in aberrant turnover dynamics, thus establishing the interdependent functional relationship between Dyn2 and the effectors TarP and TmeA.</p>
Distribution of Chlamydia trachomatis ompA-genotypes over three decades (1990-2021) in Portugal - ompA sequence datasets
<p>This repository includes sequence data from the study: "<strong>Distribution of <em>Chlamydia trachomatis</em> <em>ompA</em>-genotypes over three decades (1990-2021) in Portugal</strong>”, conducted by the <strong>National Reference Laboratory (NRL) for Sexually Transmitted Infections (STI), National Institute of Health Doutor Ricardo Jorge (INSA), Portugal</strong>.</p> <p>The NRL performs molecular characterization (namely <em>ompA</em>-genotyping) of all <em>C. trachomatis</em> positive samples that receives. <em>C. trachomatis</em> <em>ompA</em>-genotyping technique was adapted from Lan, et al. [1]. Briefly, PCR and nested PCR were performed using primers NLO and NRO, and primers PCTM3 and SERO2A, respectively, as previously described [2]. Partial nucleotide sequencing of the ~1010 bp PCR product was performed with BigDye terminator v1.1 and capillary sequencing (3130XL Genetic Analyzer; Applied Biosystems), using either two primers, as described elsewhere [2,3,4], or one primer (since ~2018), as described elsewhere [5]. Until 2018, LaserGene (DNASTAR) and MEGA (http://www.megasoftware.net) software were applied for sequence curation, alignment and phylogenetic reconstructions involving multiple reference sequences, as previously described [3]. Since ~2018, <em>ompA</em> genotypes have been determined by BLASTn-based comparison (using the ABRIcate tool [6]) directly from raw Sanger (AB1 format) sequences, with a custom database enrolling reference and variant sequences of all main <em>ompA </em>genotypes (<a href="https://github.com/insapathogenomics/ReporType/blob/main/databases/c_trachomatis.fasta">https://github.com/insapathogenomics/ReporType/blob/main/databases/c_trachomatis.fasta</a>) [3, 5, 7, 8], as currently implemented in <strong>ReporType</strong> (<a href="https://github.com/insapathogenomics/ReporType">https://github.com/insapathogenomics/ReporType</a>) [8]. When needed, MEGA software is then applied for fine genotype confirmation, namely for L2/L2b discrimination and confirmation of the hybrid <em>ompA</em>-profile of the recombinant L2b/D-Da [5].</p> <p>This repository includes the following sequence datasets:</p> <ul> <li><strong>Dataset 1</strong> - <em>ompA</em> sequences (curated FASTA) of <em>C. trachomatis</em> positive samples collected between 1991 and ~2018, as described above.</li> <li><strong>Dataset 2</strong> - <em>ompA</em> sequences (raw Sanger sequences, converted from “ab1” format to FASTA) of <em>C. trachomatis</em> positive samples collected since ~2018 and 2021, as described above.</li> </ul> <p><em>Note: The associated metadata is described in the Supplementary table 2 of the manuscript. These sequence datasets do not cover genotyped samples for which the ompA sequences were lost over the three decades of the laboratory's historical collection.</em></p> <p> </p> <p>References</p> <p>1. Lan J, Ossewaarde JM, Walboomers JM, Meijer CJ, van den Brule AJ. Improved PCR sensitivity for direct genotyping of Chlamydia trachomatis serovars by using a nested PCR. <em>J Clin Microbiol</em>. 1994;32(2):528-530. doi:10.1128/jcm.32.2.528-530.1994;</p> <p>2. Gomes JP, Bruno WJ, Borrego MJ, Dean D. Recombination in the genome of <em>Chlamydia trachomatis </em>involving the polymorphic membrane protein C gene relative to <em>ompA </em>and evidence for horizontal gene transfer. <em>J Bacteriol </em>2004;186:4295–4306;</p> <p>3. Nunes A, Borrego MJ, Nunes B, Florindo C, Gomes JP. Evolutionary dynamics of <em>ompA</em>, the gene encoding the <em>Chlamydia trachomatis</em> key antigen. J Bacteriol. 2009 Dec;191(23):7182-92. doi: 10.1128/JB.00895-09. Epub 2009 Sep 25. PMID: 19783629; PMCID: PMC2786549;</p> <p>4. Nunes A, Nogueira PJ, Borrego MJ, Gomes JP. Adaptive evolution of the Chlamydia trachomatis dominant antigen reveals distinct evolutionary scenarios for B- and T-cell epitopes: worldwide survey. PLoS One. 2010 Oct 5;5(10):e13171. doi: 10.1371/journal.pone.0013171. PMID: 20957150; PMCID: PMC2950151.</p> <p>5. Borges V, Cordeiro D, Salas AI, et al. <em>Chlamydia trachomatis</em>: when the virulence-associated genome backbone imports a prevalence-associated major antigen signature. <em>Microb Genom</em>. 2019;5(11):e000313. doi:10.1099/mgen.0.000313;</p> <p>6. Seemann T. ABRIcate. <a href="https://github.com/tseemann/abricate">https://github.com/tseemann/abricate</a></p> <p>7 Nunes A, Nogueira PJ, Borrego MJ, Gomes JP. Adaptive evolution of the Chlamydia trachomatis dominant antigen reveals distinct evolutionary scenarios for B- and T-cell epitopes: worldwide survey. PLoS One. 2010 Oct 5;5(10):e13171. doi: 10.1371/journal.pone.0013171. PMID: 20957150; PMCID: PMC2950151.</p> <p>8. Cruz H, Pinheiro M, Borges V. ReporType: a flexible bioinformatics tool for targeted loci screening and typing of infectious agents (<a href="https://github.com/insapathogenomics/ReporType">https://github.com/insapathogenomics/ReporType</a>). Int J Mol Sci. 2024;25:3172. https://doi.org/10.3390/ijms25063172</p>
Evaluation of EVO100 for Prevention of Urogenital Chlamydia Trachomatis and Neisseria Gonorrhoeae Infection
ClinicalTrials.gov study NCT04553068. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Use of Abbott RealTime CT/NG to Detect Chlamydia Trachomatis [CT] & Neisseria Gonorrhoeae [NG] in Men Who Have Sex With Men [MSM]
ClinicalTrials.gov study NCT01291264. IPD Sharing: Not stated. Countries: 1. Publications: 11.
Antenatal Chlamydia Trachomatis and Neisseria Gonorrhoeae Testing to Prevent Adverse Neonatal Consequences
ClinicalTrials.gov study NCT04955717. IPD Sharing: NO. Countries: 2. Publications: 2.
Distinct roles of the Chlamydia trachomatis effectors TarP and TmeA in the regulation of formin and Arp2/3 during entry
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Dynamin-dependent entry of Chlamydia trachomatis is sequentially regulated by the effectors TarP and TmeA
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Data from: A platform supporting generation and isolation of random transposon mutants in Chlamydia trachomatis
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Are Rectal and Genital Chlamydia Trachomatis Infections in Women Related to Anal Sex, Autoinoculation / Contamination
ClinicalTrials.gov study NCT04030949. IPD Sharing: NO. Countries: 1. Publications: 11.
Home Sampling Versus Conventional Sampling for Screening of Urogenital Chlamydia Trachomatis in Young Men and Women.
ClinicalTrials.gov study NCT00283127. IPD Sharing: Not stated. Countries: 1. Publications: 7.
Community-Based Trial of Screening for Chlamydia Trachomatis to Prevent Pelvic Inflammatory Disease
ClinicalTrials.gov study NCT00115388. IPD Sharing: Not stated. Countries: 1. Publications: 4.
Safety and Efficacy Study of Rifalazil for the Treatment of Chlamydia Trachomatis Infection in Women
ClinicalTrials.gov study NCT01631201. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Prevention of Diseases Induced by Chlamydia Trachomatis
ClinicalTrials.gov study NCT02904811. IPD Sharing: NO. Countries: 1. Publications: 2.
Randomized Population-Based Study on Chlamydia Trachomatis Screening
ClinicalTrials.gov study NCT00827970. IPD Sharing: Not stated. Countries: 1. Publications: 2.
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