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89 results for “Chlamydomonas reinhardtii”
Plasmid Maps for a Nuclear Transformation Vector in Chlamydomonas reinhardtii for the Expression and Secretion of the Plastic-Degrading Enzyme (PHL7)
<p><strong>pJP32PHL7 Vector:</strong></p> <ul> <li> <p><strong>Size:</strong> 5692 bp</p> </li> <li> <p><strong>Key Features:</strong></p> <ul> <li><strong>HSP70 Promoter:</strong> A heat shock protein promoter fused with the <em>rbcS2</em> promoter to drive expression of downstream genes.</li> <li><strong>Ble Resistance Gene:</strong> Confers resistance to bleomycin, useful for selection in <em>Chlamydomonas reinhardtii</em>.</li> <li><strong>PHL7 Gene:</strong> Encodes the plastic-degrading enzyme PHL7, inserted downstream of the <em>F2A</em> site for expression in the host.</li> <li><strong>Intron Sequences:</strong> Contains multiple <em>rbcS2</em> introns for enhancing expression in <em>Chlamydomonas</em>.</li> <li><strong>Selectable Marker (AmpR):</strong> Confers ampicillin resistance for selection in <em>E. coli</em>.</li> <li><strong>Replication Origin:</strong> Includes <em>ori</em> and <em>F1 ori</em> for replication in <em>E. coli</em>.</li> </ul> <p> </p> </li> <li> <p><strong>Applications:</strong> This vector is designed for nuclear transformation in <em>Chlamydomonas reinhardtii</em>, enabling the expression and secretion of the plastic-degrading enzyme (PHL7) under the control of a hybrid <em>HSP70</em>rbcS2 promoter.</p> </li> </ul> <p><strong>pJP32PHL7dg Vector:</strong></p> <ul> <li> <p><strong>Size:</strong> 5692 bp</p> </li> <li> <p><strong>Key Features:</strong></p> <ul> <li><strong>HSP70 Promoter:</strong> Retains the HSP70 and <em>rbcS2</em> fusion promoter for gene expression.</li> <li><strong>LacZ Alpha Fragment:</strong> Includes a LacZ alpha fragment for blue/white screening.</li> <li><strong>PHL7 Gene:</strong> Encodes the plastic-degrading enzyme PHL7, linked downstream of the <em>F2A</em> site, allowing for expression in the host.</li> <li><strong>Ble Resistance Gene:</strong> Also confers bleomycin resistance for selection in <em>Chlamydomonas</em>.</li> <li><strong>Selectable Marker (AmpR):</strong> Confers ampicillin resistance for selection in <em>E. coli</em>.</li> <li><strong>Intron Sequences:</strong> Contains <em>rbcS2</em> introns for optimizing gene expression in the host organism.</li> </ul> <p> </p> </li> <li> <p><strong>Applications:</strong> The pJP32PHL7dg vector is similarly designed for nuclear transformation in <em>Chlamydomonas reinhardtii.</em> It also facilitates the expression and secretion of the plastic-degrading enzyme PHL7, driven by the hybrid <em>HSP70</em>rbcS2 promoter, but without glycosilation sites.</p> </li> </ul>
Competitive growth experiments with a high-lipid Chlamydomonas reinhardtii mutant strain and its wild-type to predict industrial and ecological risks
<p>Key microalgal species are currently being exploited as biomanufacturing platforms using mass cultivation systems. The opportunities to enhance productivity levels or produce non-native compounds are increasing as genetic manipulation and metabolic engineering tools are rapidly advancing. Regardless of the end product, there are both environmental and industrial risks associated to open pond cultivation of mutant microalgal strains. A mutant escape could be detrimental to local biodiversity and increase the risk of algal blooms. Similarly, if the cultivation pond is invaded by a wild-type microalgae or the mutant reverts to wild-type phenotypes, productivity could be impacted. To investigate these potential risks, a response surface methodology was applied to determine the competitive outcome of two <em>Chlamydomonas reinhardtii</em> strains, a wild-type (CC-124) and a high-lipid accumulating mutant (CC-4333), grown in mixotrophic conditions, with differing levels of nitrogen and initial wild-type to mutant ratios. Results of the growth experiments show that mutant cells have double the exponential growth rate of the wild-type in monoculture. However, due to a slower transition from lag phase to exponential phase, mutant cells are outcompeted by the wild-type in every co-culture treatment. This suggests that, under the conditions tested, outdoor cultivation of the <em>C. reinhardtii</em> cell wall-deficient mutant strains does not carry a significant environmental risk to its wild-type in an escape scenario. Furthermore, lipid results show the mutant strain accumulates over 200% more TAGs per cell, at 50 mg/L NH<sub>4</sub>Cl, compared to the wild-type, therefore, the fragility of the mutant strain could impact on overall industrial productivity.</p>
In silico identified signal peptides of Chlamydomonas reinhardtii
<p><strong>Overview</strong></p> <p><em>Chlamydomonas reinhardtii </em>theoretical signal peptides identified by<em> </em>SignalP 4.0 in a protein data set described below:</p> <ul> <li>Protein data set came from "The Genome Portal of the Department of Energy Joint Genome Institute" (http://genome.jgi.doe.gov/)</li> <li>Protein sequences were evaluated in SignalP 4.0 Server (http://www.cbs.dtu.dk/services/SignalP/)</li> </ul> <p> </p> <p><strong>File used</strong></p> <p>Chlre4_best_proteins.fasta.gz -> Protein dataset version used for analysis</p> <p> </p> <p><strong>Workflow</strong> </p> <p> ______Chlre4_best_proteins.fasta.gz_______</p> <p> | |</p> <p> Chlre4_best_proteins_fasta_protein_woSP.fasta Chlre4_best_proteins_signalPeptide.fasta</p> <p> |</p> <p> ___Chlre4_best_proteins_signalPeptide_unique.fasta___</p> <p> | |</p> <p> Chlre4_best_proteins_signalPeptide_unique.aln Signal Peptide Anotation from aligned.xlsx</p> <p> </p> <p><strong>Info</strong></p> <p>Chlre4_best_proteins_fasta_protein_woSP.fasta -> Mature protein sequences from proteins identified without signal peptide</p> <p>Chlre4_best_proteins_signalPeptide.fasta -> Identified signal peptide</p> <p>Chlre4_best_proteins_signalPeptide_unique.fasta -> Unique identified signal peptide</p> <p>Chlre4_best_proteins_signalPeptide_unique.aln -> Align signal peptides (UGENE)</p> <p>Signal Peptide Annotation from aligned.xlsx -> Signal peptide list, highlighted in orange theoretical tested.</p> <p> </p> <p><strong>Citations</strong></p> <p>For use of signal peptide dataset, please cite:</p> <p>Molino JVD, de Carvalho JCM, Mayfield SP (2018) Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS ONE 13(2): e0192433. https://doi.org/10.1371/journal. pone.0192433</p> <p>and </p> <p><strong>SignalP 4.0: discriminating signal peptides from transmembrane regions</strong><br> Thomas Nordahl Petersen, Søren Brunak, Gunnar von Heijne & Henrik Nielsen<br> <em>Nature Methods</em>, <strong>8</strong>:785-786, <strong>2011</strong><br> <br> doi: 10.1038/nmeth.1701<br> PMID: 21959131<br> Supplementary materials: nmeth.1701-S1.pd</p> <p>and </p> <p><strong>The genome portal of the Department of Energy Joint Genome Institute: 2014 updates</strong></p> <p>H. Nordberg, M. Cantor, S. Dusheyko, S. Hua, A. Poliakov, I. Shabalov, T. Smirnova, I. V. Grigoriev, I. Dubchak, ,</p> <p>Nucleic Acids Res. 42, 26–31. <strong>2014</strong> </p> <p>doi:10.1093/nar/gkt1069.</p> <p> </p>
Dataset for "Antisense transcription from neighboring genes interferes with the expression of mNeonGreen as a functional in vivo fluorescent reporter in the chloroplast of Chlamydomonas reinhardtii."
<p>Plasmid sequences for "Antisense transcription from neighboring genes interferes with the expression of mNeonGreen as a functional in vivo fluorescent reporter in the chloroplast of Chlamydomonas reinhardtii."</p>
Intelligent image-activated sorting of Chlamydomonas reinhardtii by mitochondrial localization
<p>Data for image-based cell sorting based on complex intracellular morphology taken with an imaging flow cytometer</p> <p>Includes: </p> <p>Flow cytometry images of two strains of microalgae </p> <p>6-layer CNN model used for real-time cell sorting</p> <p>meta-data for the CNN containing the parameters used for creating the CNN model</p> <p>Validation images of the image-based sorting experiments.</p>
Seven Fluorescent proteins profile in Chlamydomonas reinhardtii and R code statistic for analysis
<p><strong>Overview</strong></p> <p>Data points present in this dataset were obtained following the protocol described in dx.doi.org/10.17504/protocols.io.kfnctme. We picked transformed colonies and cultured in 400 μL TAP medium for 7 days in Deep-well plates (Corning Axygen®, No.: PDW500CS, Thermo Fisher Scientific Inc., Waltham, MA), covered with Breathe-Easy® (Sigma-Aldrich®). Cultivation was performed on a rotary shaker, set to 150 rpm, under constant illumination (50 μmol photons/m<sup>2</sup>s). Then 100 μL sample were transferred clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA) and fluorescence was measured using an Infinite® M200 PRO plate reader (Tecan, Männedorf, Switzerland). Supernatant samples were obtained by spinning Deep-well plates at 3000 × <em>g</em> for 10 min and transferring 100 μL from each well to the clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA), followed by fluorescence measurement. To compare the constructs, R Statistic version 3.3.3 was used to perform one-way ANOVA (with Tukey's test), and to test statistical hypotheses, the significance level was set at 0.05. Graphs were generated in RStudio v1.0.136. The codes are deposit herein.</p> <p><strong>Info</strong></p> <p>ANOVA_Turkey_Sub.R -> code for ANOVA analysis in R statistic 3.3.3</p> <p>Anova_Output_Summary_Guide.pdf -> Explain the ANOVA files content</p> <p>Analysis_Raw_FP.xlsx -> File with raw values organized in a spreadsheet </p> <p>pRFU_<strong>FLUORESCENT PROTEIN</strong>_+_bk.csv -> relative supernatant mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii </em></p> <p>sup_RFU_<strong>FLUORESCENT PROTEIN_</strong>+_bk.csv -> supernatant mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> </p> <p>who_RFU_<strong>FLUORESCENT PROTEIN_</strong>+_bk.csv -> whole culture mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> </p> <p>pRFU_<strong>FLUORESCENT PROTEIN</strong>_+_bk.doc -> ANOVA of relative supernatant mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> </p> <p>sup_RFU_<strong>FLUORESCENT PROTEIN_</strong>+_bk.doc -> ANOVA of supernatant mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> </p> <p>who_RFU_<strong>FLUORESCENT PROTEIN_</strong>+_bk.doc -> ANOVA of whole culture mCherry fluorescence dataset of positive colonies, blanked with parental wild-type cc1690 cell of <em>Chlamydomonas reinhardtii</em> </p> <p> </p> <p><strong>Consider citing our work. </strong></p> <p>1. Molino JVD, de Carvalho JCM, Mayfield S. Evaluation of secretion reporters to microalgae biotechnology: blue to red fluorescent proteins. Algal Res. 2018;31: 252–261. doi:10.1016/j.algal.2018.02.018</p>
The effects of sex on extinction dynamics of Chlamydomonas reinhardtii depend on the rate of environmental change
<p>The continued existence of sex, despite many the costs it entails, still lacks an adequate explanation, as previous studies demonstrated that the effects of sex are environment-dependent: sex enhances the rate of adaptation in changing environments, but the benefits level off in benign conditions. To the best of our knowledge, the potential impact of different patterns of environmental change on the magnitude of these benefits received less attention in theoretical studies. In this paper, we begin to explore this issue by examining the effect of the rate of environmental deterioration (negatively correlated with population survival rate), on the benefits of sex.</p> <p>To investigate the interplay of sex and the rate of environmental deterioration, we carried out a long-term selection experiment with a unicellular alga (<em>Chlamydomonas</em> <em>reinhardtii</em>), by manipulating mode of reproduction (asexual, facultative or obligate sexual) and the rate of environmental deterioration (an increase of salt concentration). We monitored both the population size and extinction dynamics.</p> <p>The results revealed that the relative advantage of sex increased at the intermediate rate and plateaued at the highest rate of environmental deterioration. Obligate sexual populations had the slowest extinction rate under the intermediate rate of environmental deterioration, while facultative sexuality was favoured under the high rate-treatment.</p> <p>To the best of our knowledge, our study is the first to demonstrate that the interplay of sex and the rate of environmental deterioration affects the probability of survival, which indicates that mode of reproduction may be an important determinant of survival of the anthropogenic-induced environmental change.</p>
The effects of sex on extinction dynamics of Chlamydomonas reinhardtii depend on the rate of environmental change
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Dataset of phototactic response kinetics of Chlamydomonas reinhardtii
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Compartmentalized sesquiterpenoid biosynthesis and functionalization in the Chlamydomonas reinhardtii plastid
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Data from: Whole genome resequencing reveals extensive natural variation in the model green alga Chlamydomonas reinhardtii
We performed whole-genome resequencing of 12 field isolates and eight commonly studied laboratory strains of the model organism Chlamydomonas reinhardtii to characterize genomic diversity and provide a resource for studies of natural variation. Our data support previous observations that Chlamydomonas is among the most diverse eukaryotic species. Nucleotide diversity is ∼3% and is geographically structured in North America with some evidence of admixture among sampling locales. Examination of predicted loss-of-function mutations in field isolates indicates conservation of genes associated with core cellular functions, while genes in large gene families and poorly characterized genes show a greater incidence of major effect mutations. De novo assembly of unmapped reads recovered genes in the field isolates that are absent from the CC-503 assembly. The laboratory reference strains show a genomic pattern of polymorphism consistent with their origin as the recombinant progeny of a diploid zygospore. Large duplications or amplifications are a prominent feature of laboratory strains and appear to have originated under laboratory culture. Extensive natural variation offers a new source of genetic diversity for studies of Chlamydomonas, including naturally occurring alleles that may prove useful in studies of gene function and the dissection of quantitative genetic traits.
Fluorescence microscopy of Chlamydomonas reinhardtii for mCherry detection: secretion peptides strains.
<p><strong>Overview</strong></p> <p>Life-cell imaging was performed with a confocal fluorescence microscopy to observe mCherry in the secretion vacuoles. mCherry fluorescence compartmentalization was observed by a Confocal Zeiss LSM 780-NLO, using an argon laser 543 nm to excite mCherry and a spectral detector set approximately to 610-650 nm range. For chlorophyll, we used a laser at 405 nm for excitation, and spectral detector set to 680 nm region. All pictures were taken with the same system configuration and analyzed by Fiji, an ImageJ distribution software. Cells images were acquired in bundles of 0.4 μm afar photos per channel in the z-axis. </p> <p> </p> <p><strong>Files info:</strong></p> <p>Each file is the raw image obtained from fluorescent microscopy.</p> <p> </p> <p><strong>Organization</strong></p> <p>Construct_name.czi - Ex: "pAH04mCherry.czi"</p> <p>pAH04mCherry -> construct without signal peptide</p> <p>pJP22mCherry -> construct with signal peptide from arylsulfatase 1 (<em>Chlamydomonas </em><em>reinhardtii</em>)</p> <p>pJP26mCherry -> construct with signal peptide from binding protein 1 (<em>C. </em><em>reinhardtii</em>)</p> <p>pJP28mCherry -> construct with signal peptide from carbonic anhydrase 1 (<em>C. </em><em>reinhardtii</em>)</p> <p>pJP29 mCherry -> construct with signal peptide from ice-binding protein 1 (Artic <em>Chlamydomonas sp</em>)</p> <p>pJP30-35mCherry -> construct with signal peptide from in silico identified list (DOI 10.5281/zenodo.556792).</p> <p>Wildtype cc1690 -> parental strain used for transformation.</p> <p> </p> <p>For more information on the constructs, check our paper. </p> <p> </p> <p><strong>Consider citing our work. </strong></p> <p>Molino JVD, de Carvalho JCM, Mayfield SP (2018) Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS ONE 13(2): e0192433. https://doi.org/10.1371/journal. pone.0192433</p> <p> </p>
Cell size, chlorophyll fluorescence and cadmium bioaccumulation between wild-type and mutant strains of Chlamydomonas reinhardtii upon exposure to cadmium
<p>Heavy metal contamination presents a constant threat to biological systems. Simultaneously, heavy metals have become one of the major contaminants in the aquatic ecosystem. In this regard, the investigation of heavy metal-tolerance genes in algae is relevant. <i>Chlamydomonas reinhardtii</i> is a unicellular green alga, and an excellent model organism used in heavy metal studies. In <i>C. reinhardtii</i>, a novel gene designated as Cia7, was hypothesized to play a role in heavy metal homeostasis due to CIA7's conserved cysteine-residue motif. This study compared two strains of <i>C. reinhardtii</i>, cc4425, the wild-type possessing the functional CIA7 protein and cc5013, the mutant strain possessing the disrupted cia7- gene. The hypothesis was that the expression of Cia7 contributes to an increased cadmium (Cd)-tolerance in <i>C. reinhardtii</i>. The Cd-tolerance would be described by physiological markers of microalgae health, and by intracellular accumulation of the metal. Methods: The objectives of this study were (1) to compare chlorophyll fluorescence and cell size in cc4425 and cc5013 exposed to Cd<sup>2+</sup>, and (2) to compare Cd<sup>2+</sup> bioaccumulation in cc4425 and cc5013 strains in different growth media. Flow cytometry, and inductively coupled plasma optical emission spectrometry (ICP-OES) analysis were performed. Results: There was no significant statistical difference in Cd<sup>2+</sup> bioaccumulation between the two strains, cc4425 and cc5013, regardless of growth media. However, a statistically significant difference in Cd<sup>2+</sup> bioaccumulation (p<0.0001) was determined between the media (with acetate and without acetate). The cia7- mutant, cc5013 was found to be more susceptible to a Cd<sup>2+</sup>-induced decrease in chlorophyll fluorescence and had a reduced cell size compared to cc4425, the wild-type strain. Conclusions: These observed differences between the strains suggest that CIA7's biological activity could play a direct or indirect role in increasing Cd tolerance in <i>C. reinhardtii</i>.</p>
A PETase enzyme synthesised in the chloroplast of the microalga Chlamydomonas reinhardtii is active against PET and polystyrene
<p>The list contains:</p> <p>1) raw data for chromatographies: HiprepSPHP and SEC</p> <p>2) raw data for UV-vis Spectrum</p> <p>3) raw data for all MS/MS spectra manuscript + supplementary</p> <p>4) raw data for AFM </p> <p>5) report data from HPLC</p>
Data from: Whole genome resequencing reveals extensive natural variation in the model green alga Chlamydomonas reinhardtii
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Cell size, chlorophyll fluorescence and cadmium bioaccumulation between wild-type and mutant strains of Chlamydomonas reinhardtii upon exposure to cadmium
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Data from: Positive size-speed relationships in gametes and vegetative cells of Chlamydomonas reinhardtii; implications for the evolution of sperm.
It is commonly held that differences in gametes of the two sexes (anisogamy) evolved from ancestors whose gametes were similar in size and behaviour (isogamy). Underlying many hypotheses explaining anisogamy are assumed relationships between cell size and speed in the ancestral isogamous population. Using the isogamous alga Chlamydomonas reinhardtii, we explored size-speed distributions in vegetative and gamete cells of ten cell lines, and clonal data from within two cell lines. We applied an independent speed selection approach to gamete populations of C. reinhardtii, monitoring correlated responses in size following selection for high speed. We demonstrate positive size-speed relationships in clones, cell lines and artificially selected speed selection lines. We found different size-speed relationships in the two cell types of C. reinhardtii even though they overlap in size, suggesting that cell composition and/or programs of gene expression are capable of altering this relationship, and that the relationship is evolvable. The positive genetic size-speed correlation means that the division of parent vegetative cells into numerous gametes trades off against not only size but also speed, a trade-off that has not received previous attention. Our results support re-evaluating the role of speed selection in the evolution of anisogamy.
Raw data of: Settling selection of <i>Chlamydomonas reinhardtii</i> for samarium uptake.
<p>Data are the raw results of the investigation entitled, Settling selection of <em>Chlamydomonas reinhardtii</em> for samarium uptake.</p> <p>This study uses an experimentally evolved <em>C. reinhardtii </em>(Martínez-Alesón García et al., 2021), selected under Samarium and acid pH conditions. We carry out further selection procedures to increase Sm sequestration. Control, ancestral, and selected populations of <em>C. reinhardtii </em>were characterized using a Coulter Counter multisizer 4, FlowCam 3.0, using Transmission electron microscopy<em> </em>(TEM), ray spectroscopy (Energy Dispersive X-ray (EDX)) and with an Imaging PAM fluorimeter. The populations studies were a <em>C. reinhardtii</em> wild-type control (control strain), Sm-uptake candidate strain (CSm0 strain) resulting from mixing thirteen Sm-adapted strains (refer to Martínez-Alesón García et al., 2021), and newly established strains label for the selection cycle as CSm1, CSm2, CSm3, and CSm4 strains.</p> <p><strong><em>Files:</em></strong></p> <ul> <li>Coulter Counter particles distributions of all strains: <ul> <li><a href="../api/records/8392917/draft/files/Coulter%20Counter%20raw%20data.zip/content" target="_blank" rel="noopener noreferrer">Coulter Counter raw data.zip</a> Data from Control, CSm0, CSm1, CSm2, CSm3, and CSm4 strains grown in BG11 medium under laboratory conditions for 15 days.</li> </ul> </li> <li>Microscopic images: <ul> <li><a href="../api/records/8392917/draft/files/Flow%20Cam%20raw%20images%20and%20files.zip/content" target="_blank" rel="noopener noreferrer">Flow Cam raw images and files.zip</a> FlowCam 3.0 Microphotographs and files were generated for all strains grown in BG11 medium under laboratory conditions for 15 days. </li> <li><a href="8392917" target="_blank" rel="noopener noreferrer">Optic Microscopy microphotographs.zip</a> Optic Microscopy Microphotographs measure morphological traits with ImageJ in CSm0 and CSm4 strains.</li> <li><a href="8392917" target="_blank" rel="noopener noreferrer">TEM Images.zip</a> Transmission electron microscopy<em> </em>(TEM) pictures were obtained with an electron microscope, JEOL-JEM 1400 (Jeol Ltd., Tokyo, Japan) from control and CSm4 strains.</li> </ul> </li> <li>Data files generated from microscopy images:<br> <ul> <li><a href="../api/records/8392917/draft/files/ImageJ%20analyses%20Raw%20data.xlsx/content" target="_blank" rel="noopener noreferrer">ImageJ analyses Raw data.xlsx</a>: ImageJ analyses files.</li> <li><a href="../api/records/8392917/draft/files/TEM-EDX%20analyses.zip/content" target="_blank" rel="noopener noreferrer">TEM-EDX analyses.zip</a> Ray spectroscopy (Energy Dispersive X-ray (EDX)) spectrums obtained from TEM cuts.</li> </ul> </li> <li><a href="../api/records/8392917/draft/files/PAM%20raw%20data.zip/content" target="_blank" rel="noopener noreferrer">PAM raw data.zip</a> PAM fluorescence Raw data of </li> </ul>
SI Reduction of phosphoribulokinase amount and re-routing me-tabolism in Chlamydomonas reinhardtii CP12 mutants.
<p>Supplementary material associated with the manuscript<strong>: </strong>Reduction of phosphoribulokinase amount and re-routing me-tabolism in Chlamydomonas reinhardtii CP12 mutants</p>
Data from: The experimental evolution of herbicide-resistance in Chlamydomonas reinhardtii results in a positive correlation between fitness in the presence and absence of herbicides
Pleiotropic fitness trade-offs will be key determinants of the evolutionary dynamics of selection for pesticide resistance. However, for herbicide resistance, empirical support for a fitness cost of resistance is mixed, and it is therefore also questionable what further ecological trade-offs can be assumed to apply to herbicide resistance. Here, we test the existence of trade-offs by experimentally evolving herbicide resistance in Chlamydomonas reinhardtii. Although fitness costs are detected for all herbicides, we find that, counterintuitively, the most resistant populations also have the lowest fitness costs as measured by growth rate in the ancestral environment. Furthermore, after controlling for differences in the evolutionary dynamics of resistance to different herbicides, we also detect significant positive correlations between resistance, fitness in the ancestral environment and cross-resistance to other herbicides. We attribute this to the highest levels of nontarget-site resistance being achieved by fixing mutations that more broadly affect cellular physiology, which results in both more cross-resistance and less overall antagonistic pleiotropy on maximum growth rate. Consequently, the lack of classical ecological trade-offs could present a major challenge for herbicide resistance management.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.