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13 results for “Colletotrichum gloeosporioides”
Fig. 7 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 7. Phylogenetic relationship of the five FnTPS candidates with other known terpene synthases and a sequence alignment showing the conserved protein motifs. (A) A maximum-likelihood tree of the TPS proteins depicting the TPS-a, TPS-b, TPS-d, TPS-e/f, and TPS-g clades, with bootstrap values greater than 50% shown for the branching. The scale bar corresponds to 6% amino acid substitution. The five F. nilgerrensis proteins are in red. Selected proteins with available three-dimensional structural data are shown in bold using their PDB code followed by the abbreviated species name. The known enzymatic products are in light blue followed with the UniProt Accession numbers of the proteins. (B) Alignment of the five candidates FnTPSs with α-farnesene synthase from apple (Malus domestica) and α-bergamotene synthase from Lavender (Lavandula Angustifolia). The DxDD motif of typical class II terpene synthases in FnTPS6 is boxed and the highly conserved class I DDxxD as well as the lesser conserved RRx8W and NSE/DTE motifs are indicated. The color regime of amino acids is set in Bioedit version 7.2.6. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 3 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 3. Most enriched metabolic pathways of the DEGs during the first 18 hpi with C. gloeosporioides. Only significant pathways with a Q-value below 1 are shown. Terpene metabolism clusters are highlighted.
Fig. 1 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 1. Determination of terpenoids in leaves challenged with C. gloeosporioides. (A) Total contents of monoterpenes and sesquiterpenes, (B–C) The contents of individual monoterpene and sesquiterpene identified. Data are average of two independent experiments.
Fig. 4 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 4. Schematic illustration of general terpenoid biosynthesis pathways showing identified unigenes from F. nilgerrensis leaf transcriptome data. The interconvertible precursors IPP and DMAPP, two phosphorylated C5 unites, are produced by the MVA and MEP pathways which are exchangeable from both compartmentations. The chloroplast is a major site for synthesis of hemiterpene (C5), monoterpenoids (C10), diterpenoids (C20) carotenoids (C40) and chlorophyll, while the cytosol and other organelle are responsible for synthesis of monoterpenoids (C10), sesquiterpene (C15) and triterpene (C30). But that is not strictly conclusive. Arrow with lines indicate reactions catalyzed by enzymes and the encoding genes, with unigenes identified in this experiment boxed. The color highlights are for better visualization. Abbreviations: AACT, acetoacetyl-CoA thiolase; CMK, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; DXR, 1-deoxy-D-xylulose 5-phosphate reductase; DXS, 1-deoxy-D-xylulose 5-phosphate synthase; HDR, (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate reductase; HDS, (E)-4-hydroxy-3- methyl-but-2-enyl diphosphate synthase; HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; HMGS, 3-hydroxy-3-methylglutaryl-CoA synthase; IDI, isopentenyl diphosphate isomerase; MCT, MEP cytidyltransferase; MDC, mevalonate-5-diphosphate decarboxylase; MDS, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MVK, mevalonate kinase. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 2 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 2. Global differentially-expressed-genes (DEG) in leaves of F. nilgerrensis after challenged by C. gloeosporioides. (A) DEG heatmap depicts clustering of the early upregulated genes (up to 18-h post challenge) and the late respondents (>24 h) in the upper and lower parts, respectively, with top GO terms indicated. (B) Major GO term enrichment in an early responsive gene cluster 7 showing that terpenoid biosynthesis genes are co-expressed with antipathogen immune-responsive pathway genes. Each time-point contains 2 biological replications and the expression data was zero-normalized before hierarchical clustering is performed (Pearson uncentered metric, average linkage ordering) using Cluster 3.0 version 1.58. Heatmap was examined and generated using Java Tree- View version 1.1.6r4. Gene Ontology (GO) enrichment analysis of the DEGs is implemented by the topGO R package-based Kolmogorov–Smirnov test.
Fig. 5 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 5. Heat maps depicting the transcript levels of terpenoid biosynthesis genes from F. nilgerrensis leaf transcriptome in response to C. gloeosporioides. (A) Fifty-six MVA and MEP pathway-associated genes. Annotation of the unigenes by homologous are listed in Table S4. (B) Fifty-nine terpene synthase-like or related genes. Annotation of the unigenes by homologous are listed in Table S5. Average FPKM (n = 2) were log2 transformed. The heatmap was generated by Prism GraphPad version 7.04.
Global transcriptome analysis of the pH regulator pacC in Colletotrichum gloeosporioides
GEO Series GSE41844. Colletotrichum gloeosporioides. 2 samples. Type: Expression profiling by high throughput sequencing.
Identification of regulatory networks of microRNAs and their targets in immunity to Colletotrichum gloeosporioides in tea plant (Camellia sinensis L.)
GEO Series GSE119728. Camellia sinensis. 8 samples. Type: Non-coding RNA profiling by high throughput sequencing; Other.
Bacillus velezensis 83 transcriptional response during cocultivation with Colletotrichum gloeosporioides
GEO Series GSE202842. Bacillus velezensis. 12 samples. Type: Expression profiling by high throughput sequencing.
Simultaneous transcriptome analysis of Colletotrichum gloeosporioides and tomato fruits response reveals novel fungal-fruit arm and defense strategies
GEO Series GSE55553. Colletotrichum gloeosporioides; Solanum lycopersicum. 5 samples. Type: Expression profiling by high throughput sequencing.
Genome-wide identification and characterization of long non-coding RNAs conferring resistance to Colletotrichum gloeosporioides in walnut (Juglans regia)
GEO Series GSE147083. Juglans regia. 30 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Fig. 6 in Early transcriptional response of terpenoid metabolism to Colletotrichum gloeosporioides in a resistant wild strawberry Fragaria nilgerrensis
Fig. 6. Determination of gene expression of 15 selected unigenes during the time-course after C. gloeosporioides challenge via quantitative real-time PCR. The ACTIN2 gene was used as an internal reference and expression level was calculated via 2 deltaCT method. Data are the means of three independent experiments. Bar with same letters are not significantly differences as confirmed by ANOVA and one-way Student's t-test (p <0.05) in MS Excel version 2013.
Impact of exogenous caffeine on regulatory networks of microRNAs in response to Colletotrichum gloeosporioides in tea plant.
GEO Series GSE146713. Camellia sinensis. 32 samples. Type: Other; Non-coding RNA profiling by high throughput sequencing.
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