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210 results for “Cryopreservation”
Figure 4 in Use of melatonin as an inhibitor of apoptotic process for cryopreservation of zebrafish (Danio rerio) embryos
Figure 4. Representative images of zebrafish (Danio rerio) embryos showing reactive oxygen species (ROS) formation (A-D) and DNA fragmentation (E-H) following vitrification for the control group and vitrified treatments with 0, 1 µM and 1 mM melatonin respectively. Arrows and arrow heads point to tissues affected in the body axis and those in close proximity to the yolk respectively.
Figure 3 in Use of melatonin as an inhibitor of apoptotic process for cryopreservation of zebrafish (Danio rerio) embryos
Figure 3. Percentage area of zebrafish (Danio rerio) embryos affected by reactive oxygen species (ROS) formation and DNA fragmentation after vitrification for the control group and vitrified treatments with 0, 1 µM and 1 mM melatonin respectively. Bars with different letter within the same assay were significantly different from one another (P <0.05).
Figure 2 in Use of melatonin as an inhibitor of apoptotic process for cryopreservation of zebrafish (Danio rerio) embryos
Figure 2. Bax, bcl-2 and caspase-3 mRNA expression levels in zebrafish (Danio rerio) embryos for the control group and vitrified treatments with 0, 1 µM and 1 mM melatonin respectively obtained by the 2-∆∆CT method. Boxes with same letter are not significantly different from one another (P> 0.05).
Figure 1 in Use of melatonin as an inhibitor of apoptotic process for cryopreservation of zebrafish (Danio rerio) embryos
Figure 1. Representative scanning electron micrographs of vitrified and non-vitrified zebrafish (Danio rerio) embryos. (A) Control group; (B-D) Vitrified with 0, 1 µM and 1 mM melatonin respectively. Arrows, arrow head and asterisk point to invaginations and perforations, rupture of the vitelline membrane and wrinkling of the epidermis respectively.
Fig. 1 in In vitro fertilizations with cryopreserved sperm of Rhinella marina (Anura: Bufonidae) in Ecuador
Fig. 1. Rhinella marina embryo at 31 Gosner stage from in vitro fertilization with cryopreserved sperm.
FIGURE 5 in Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen
FIGURE 5 | Morphological analysis of thawed sperm from Pseudoplatystoma corruscans. A. Sperm with changes in the head, rupture of the membrane and damage to the intermediate part. B. Curled flagellum. C. Spermatozoon with damage to the head, intermediate piece and flagellum. D. Sperm head with changes in the head, damage to the membrane and damage to the intermediate part. Legends: ● = membrane rupture, * = possible cytoplasmic material, # = flagellar fibers, ♦ = cytoplasmic gout. Scale bars; A, B, and D = 2 µm; C = 5 µm.
FIGURE 4 in Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen
FIGURE 4 | Analysis of the sperm movement of thawed semen from Pseudoplatystoma corruscans. A. Total motility (MT), B. Curvilinear velocity (VCL, µm/s), C. Straight line velocity (VSL, µm/s), D. Average path velocity (VAP, µm/s). Same letters indicate no significant differences (P <0.05).
FIGURE 2 in Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen
FIGURE 2 | Fluorescence photomicrograph of nuclei with different classes of damage assessed by the Comet Assay analysis: 0, no apparent damage; 1, little damage; 2, average damage; 3, major damage. Scale bars = 95 µm.
FIGURE 1 in Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen
FIGURE 1 | Adult male specimen of Pseudoplatystoma corruscans, belonging to the Hydrobiology Station of the Jupiá Hydroelectric Plant.
FIGURE 6 in Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen
FIGURE 6 | Index of damage caused to DNA by the cryopreservation process of semen from Pseudoplatystoma corruscans. Different letters indicate significant differences (P <0.05), by the Turkey test.
FIGURE 3 in Dimethyl acetamide and dimethyl sulfoxide associated at glucose and egg yolk for cryopreservation of Pseudoplatystoma corruscans semen
FIGURE 3 | Morphological characteristics of the Pseudoplatystoma corruscans sperm. A and B, normal spermatozoa; C and D, sperm with malformations. Legends: h1, width; h2, length; mp, intermediate part; #, sperm without the flagellum; c, curled tail; rmp, reduced middle piece. Scale bars; A and C = 20 µm; B and D = 5 µm.
Cryopreservation and transplantation of common carp spermatogonia - spermatogonia viability dataset
<p><strong>Dataset of spermatogonia viability assessment from optimization of cryopreservation and vitrification protocols.</strong></p>
Efficacy and Safety of the Cryopreserved Formulation of OTL-101 in Subjects With ADA-SCID
ClinicalTrials.gov study NCT02999984. IPD Sharing: NO. Countries: 1. Publications: 2.
Oocyte Cryopreservation Registry (HOPE Registry)
ClinicalTrials.gov study NCT00699400. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Phase 1 Safety Study of Dimethyl Sulfoxide Cryopreserved Platelets
ClinicalTrials.gov study NCT02078284. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Data from: Sperm cryopreservation reduces offspring growth
Sperm cryopreservation is routinely used in reproductive medicine, livestock production, and wildlife management. Its effect on offspring performance is often assumed negligible, but this still remains to be confirmed in well controlled within-subject experiments. We use a vertebrate model that allows to experimentally separate parental and environmental effects to test whether sperm cryopreservation influences offspring phenotype under stress and non-stress conditions, and whether such effects are male-specific. Wild brown trout (Salmo trutta) were stripped for their gametes, and a portion of each male's milt was cryopreserved. Then, 960 eggs were simultaneously fertilized with either non-cryopreserved or frozen-thawed semen and raised singly in the presence or absence of a pathogen. We found no significant effects of cryopreservation on fertilization rates, and no effects on growth, survival, nor pathogen resistance during the embryo stage. However, fertilization by cryopreserved sperm lead to significantly reduced larval growth after hatching. Males varied in genetic quality as determined from offspring performance, but effects of cryopreservation on larval growth were not male specific. We conclude that cryopreservation causes a reduction of offspring growth that is easily overlooked because it only manifests itself at later developmental stages when many other factors affect growth and survival, too.
Ice recrystallization inhibitors enable efficient cryopreservation of induced pluripotent stem cells: A functional and transcriptomic analysis
<p><span>The successful use of human induced pluripotent stem cells (iPSCs) for research or clinical applications requires the development of robust, efficient, and reproducible cryopreservation protocols. After cryopreservation, the survival rate of iPSCs is suboptimal and cell line dependent. We assessed the use of ice recrystallization inhibitors (IRIs) for cryopreservation of human iPSCs<span>. A toxicity screening study was performed to assess specific small-molecule carbohydrate-based IRI and concentrations for further evaluation.</span> Then, a cryopreservation study compared the cryoprotective efficiency of 15 mM IRIs in 5 % or 10 % DMSO-containing solutions and with CryoStor® CS10. Three iPSC lines were cryopreserved as single-cell suspensions in the cryopreservation solutions and post-thaw characteristics, including pluripotency and differential gene expression, were assessed. </span><span>W</span><span>e demonstrate the fitness-for-purpose of 15 mM IRI in 5 % DMSO as an efficient cryoprotective solution for iPSCs in terms of post-thaw recovery, viability, pluripotency, and transcriptomic changes. Given that this dataset is the first report where mRNA sequencing has been used to identify expression changes resulting from iPSCs cryopreservation, it has the potential to be used for molecular mechanism analysis relating to cryopreservation. IRIs can reduce DMSO concentrations, thereby improving the utility, effectiveness, and efficiency of cryopreservation. </span></p>
A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats
<p>Mosquitoes develop in a wide range of aquatic habitats containing highly diverse and variable bacterial communities that shape both larval and adult traits, including the capacity of adult females of some mosquito species to vector disease-causing organisms to humans. However, while most mosquito studies control for host genotype and environmental conditions, the impact of microbiota variation on phenotypic outcomes of mosquitoes is often unaccounted for. The inability to conduct reproducible intra- and inter-laboratory studies of mosquito-microbiota interactions has also greatly limited our ability to identify microbial targets for mosquito-borne disease control. Here, we developed an approach to isolate and cryopreserve bacterial communities derived from lab- and field-based larval-rearing environments of the yellow fever mosquito <em>Aedes</em> <em>aegypti</em>–a primary vector of dengue, Zika, and chikungunya viruses. We then validated the use of our approach to generate experimental microcosms colonized by standardized lab- and field-derived bacterial communities. <span>Our results overall reveal minimal effects of cryopreservation on the recovery of both lab- and field-derived bacteria when directly compared with isolation from non-cryopreserved fresh material. Our results also reveal improved reproducibility of bacterial communities in replicate microcosms generated using cryopreserved stocks over fresh material. Communities in replicate microcosms further captured the majority of total bacterial diversity present in both lab- and field-based larval environments, although the relative richness of recovered taxa as compared to non-recovered taxa was substantially lower in microcosms containing field-derived bacteria. Altogether, these results provide a critical next step toward the standardization of mosquito studies to include larval-rearing environments colonized by defined microbial communities. They also lay the foundation for long-term studies of mosquito-microbe interactions and the identification and manipulation of taxa with potential to reduce mosquito vectorial capacity.</span></p>
A Safety and Efficacy Study of Cryopreserved OTL-200 for Treatment of Metachromatic Leukodystrophy (MLD)
ClinicalTrials.gov study NCT03392987. IPD Sharing: NO. Countries: 1. Publications: 1.
Clinical Pregnancies After Cryopreservation of Donor Oocytes With an Open or Closed Device
ClinicalTrials.gov study NCT02940106. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
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Allen Brain Atlas
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Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.