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56 results for “Culex pipiens”
Figure 8 in Distribution and molecular differentiation of Culex pipiens complex species in the Middle and Eastern Black Sea Regions of Turkey
Figure 8. Unrooted haplotype network for CQ11. Each circle represents a haplotype, and lines above each link indicate mutations.
Figure 7 in Distribution and molecular differentiation of Culex pipiens complex species in the Middle and Eastern Black Sea Regions of Turkey
Figure 7. Phylogenetic tree based on a 151-bp region within the CQ11 microsatellite region of Culex pipiens. The tree was constructed using the maximum likelihood method, and bootstrap values are shown as numbers on the tree.
Figure 5. Unrooted haplotype network. Each circle represents a in Distribution and molecular differentiation of Culex pipiens complex species in the Middle and Eastern Black Sea Regions of Turkey
Figure 5. Unrooted haplotype network. Each circle represents a haplotype, and the lines above each link indicate one mutation. Small black dots indicate intermediate, missing, or unsampled haplotypes.
Figure 4 in Distribution and molecular differentiation of Culex pipiens complex species in the Middle and Eastern Black Sea Regions of Turkey
Figure 4. The phylogenetic tree is based on a 651-bp region of the Ace-2 gene from Culex pipiens. The tree was constructed using the maximum likelihood method, and bootstrap values are shown as numbers on the tree.
Merged anvio profiles for Wolbachia MAGs from Culex pipiens midgut and ovary samples
<p>Anvi’o merged profile databases for <em>Wolbachia</em> MAGs from <em>Culex pipiens</em> midgut and ovary metagenomes. </p>
Fig. 6 in Molecular and physiological characterization of the chitin synthase B gene isolated from CUlex pipieNS palleNS (Diptera: Culicidae)
Fig. 6 Effect of CpCHSB silencing in third instar larvae on chitin content. a Relative chitin content in fourth-instar larvae at 72 h after siCHSB injection (n = 10). b Rhodamine B staining of the midgut of fourth-instar larvae isolated after 72 h after siCHSB injection. c Chitin staining in the midgut at 48 and 72 h after siCHSB injection (n = 10). Results are shown as the mean ± SE (Student's t-tests; *P <0.05, **P <0.01). Scale-bar: 50 μm
Fig. 5 in Molecular and physiological characterization of the chitin synthase B gene isolated from CUlex pipieNS palleNS (Diptera: Culicidae)
Fig. 5 CpCHSB gene suppression by RNAi at 1 day after injection (n = 200) in adult mosquitoes. a Expression levels of CpCHSB at 72 h after injecting siCHSB assessed by RT-qPCR. The group injected with siCHSB show a reduction in CpCHSB expression of 53% compared with the control group. b Relative chitin content at 72 h after siCHSB injection. c Midgut length at 72 h after siCHSB injection. d Number of follicles per ovary and number of eggs per female mosquito (e) after injecting siCHSB. Results are shown as the mean ± SE (Student's t-tests; **P <0.01, ***P <0.001, ns, not significant)
Fig. 2 in Molecular and physiological characterization of the chitin synthase B gene isolated from CUlex pipieNS palleNS (Diptera: Culicidae)
Fig. 2 Expression profiles of CpCHSB in different tissues of fourth-instar C. pipiens pallens larvae. Tissues include head (HE), foregut (FG), midgut (MG), hindgut (HG), Malpighian tubules (MT) and carcass (CA). Relative expression levels were calculated based on the lowest expression value, which was ascribed an arbitrary value of 1. Results are shown as the mean ± SE
Fig. 1 in Molecular and physiological characterization of the chitin synthase B gene isolated from CUlex pipieNS palleNS (Diptera: Culicidae)
Fig. 1 Alignment of the conserved catalytic domain of chitin synthases from three mosquito species Seven characteristic motifs (M1–M7) in insect chitin synthases are highlighted. Dashes are used to denote gaps introduced to maximise alignment. Abbreviations: Ae, Aedes aegypti; Ag, Anopheles gambiae; Cp, Culex pipiens pallens
Fig. 1 in German CULex pipienS biotype MoLeStUS and CULex torrentiUM are vector-competent for Usutu virus
Fig. 1 Comparison of the feeding and survival rates (from 0 to 14/16 dpi) of the four tested mosquito populations. Data values above the bars indicate the number of fully engorged or survived females per species, respectively. Numbers in brackets specify the ratio of engorged and survived females to the total number of females exposed to a blood meal or subjected to the experiment (minus day-0 samples), respectively. Error bars represent 95% confidence intervals. *P <0.05, **P <0.01, and ***P <0.001 by generalized binomial regression models or Fisher's exact test with Bonferroni correction. †Cx. pipiens biotype molestus laboratory colony from "Wendland," Lower Saxony, Germany. ‡Cx. pipiens biotype molestus laboratory colony from Novi Sad, the Republic of Serbia. §Cx. torrentium field-collected colony near Berlin and Bonn, North Rhine-Westphalia, Germany. ¶Ae. aegypti laboratory colony from Malaysia (Bayer CropScience, Langenfeld, Germany)
Fig. 3 in Influence of Hepatozoon parasites on host-seeking and host-choice behaviour of the mosquitoes Culex territans and Culex pipiens
Fig. 3. Time of mosquito biting activity on infected or uninfected frogs. Mosquitoes that fed on infected frogs are represented by the solid line, and those that fed on uninfected frogs are represented by the dashed line. The top figure represents the time for each mosquito to land in trials where mosquitoes were allowed to feed to repletion (i.e., uninterrupted trials). A significantly higher proportion of mosquitoes that fed on infected frogs began to land later in the trial, compared to mosquitoes feeding on uninfected frogs. The bottom figure represents the time for each mosquito to land in trials where mosquitoes were allow to land but were removed before feeding (i.e., interrupted trials). A significant difference in the time for mosquitoes to land on infected or uninfected frogs was not observed in interrupted trials.
Fig. 4 in Influence of Hepatozoon parasites on host-seeking and host-choice behaviour of the mosquitoes Culex territans and Culex pipiens
Fig. 4. Time between mosquito bites on infected and uninfected frogs. Mosquitoes that were allowed to feed are represented by a solid line. Mosquitoes that were aspirated off the frog after landing are represented by the dashed line. A significantly longer amount of time passed between the bite of one mosquito and the bite of the following mosquito during trials in which mosquitoes were allowed to feed on a frog, compared to trials where mosquitoes were removed before feeding.
Fig. 1 in Influence of Hepatozoon parasites on host-seeking and host-choice behaviour of the mosquitoes Culex territans and Culex pipiens
Fig. 1. Number of mosquitoes that chose wild-caught frogs paired by different levels of infection. Each panel represents a pairing by infection level in green frogs. Each trial was repeated three times, and separate trials are shown by different shapes. Higher numbers of mosquitoes chose to land on wild-caught frogs with high infections of H. clamatae when paired with frogs with moderate infections or without infection. Infection level had a weakly significant effect on the number of mosquitoes that chose to land on a frog. UI = uninfected.
Fig. 2 in Influence of Hepatozoon parasites on host-seeking and host-choice behaviour of the mosquitoes Culex territans and Culex pipiens
Fig. 2. Number of mosquitoes that chose laboratory-raised frogs that were uninfected or experimentally infected. Each panel represents a pairing of infected or uninfected green frogs. Each pairing was repeated four times and each trial is represented by a different shape. Infection level did not have a significant effect on the number of mosquitoes choosing to land on a frog.
Figure 2 in Larvicidal, and cytoxicity of Lepidium sativum L. seed extract against Culex pipiens L. (Diptera: Culicidae)
Figure 2. Percentage hatchability and activity of the ethyl acetate extract of Lepidium sativum against Culex pipiens third instar larvae treated with different concentrations. Significant differences were assessed using one-way ANOVA followed by Tukey's test, with p <0.05 considered to indicate significant differences. Different letters indicate significant differences.
Figure 4 in Larvicidal, and cytoxicity of Lepidium sativum L. seed extract against Culex pipiens L. (Diptera: Culicidae)
Figure 4. Effect of ethyl acetate extract of L. sativum on the morphology of human umbilical vein endothelial cells. The morphological and nuclear features were observed after 24 h incubation and imaged under an inverted microscope (200× magnification). The arrows point to the fragmented DNA.
Figure 3 in Larvicidal, and cytoxicity of Lepidium sativum L. seed extract against Culex pipiens L. (Diptera: Culicidae)
Figure 3. Photomicrographs of midguts of Cx. pipiens larvae demonstrating: (Chouin-Carneiro et al., 2016). Longitudinal sections in the midguts (MG) of control larvae with normal and healthy epithelial cells (Ec), microvilli (Mv), nuclei (n), and regenerative cells (Rc). Notice absence of the lesions. H&E stain. (C-D): Longitudinal sections in midguts of AzE-treated larvae, with blebbing (Bc) and protruding (Pc) of the epithelial cells into the lumen (Lu) of midgut of the larvae and degraded microvilli (DMv). H&E stain.
Figure 1 in Identification of blood meals in field collected Culex pipiens, Anopheles sacharovi and Culex tritaeniorhynchus (Diptera: Culicidae) using the ELISA method
Figure 1. Sampling localities of Anopheles sacharovi, Culex pipiens, Culex tritaeniorhynchus populations (1. Huzurkent, 2. Düziçi, 3. Akhisar, 4. Dalaman, 5. Gelendost, 6. Selçuk, 7. Karataş, 8. Eşme, 9. Türkoğlu, 10. Dörtyol, 11. Kırıkhan, 12. Manavgat, 13. Afyon, 14. Tarsus, 15. Kadirli, 16. Aydın, 17. Kozan, 18. Sandıklı, 19. Dinar, 20. Uşak, 21. Ceyhan, 22. Antalya, 23. Tuzla 24. İzmir, 25. Söke, 26. Kuşadası, 27. Akköy). Red stars indicate locations where Cx. pipiens was sampled, the green diamond shape indicates locations of Cx. tritaeniorhynchus and the blue pins indicate the locations of An. sacharovi.
Figure 2 in Identification of blood meals in field collected Culex pipiens, Anopheles sacharovi and Culex tritaeniorhynchus (Diptera: Culicidae) using the ELISA method
Figure 2. Percentage distributions of single and multiple host meal choices for three mosquito species collected in the Aegean and Mediterranean regions.
Fig. 4 in Molecular and physiological characterization of the chitin synthase B gene isolated from CUlex pipieNS palleNS (Diptera: Culicidae)
Fig. 4 RNA interference (RNAi) of CpCHSB in third-instar larvae (n = 200). a Expression levels of CpCHSB at 72 h after injecting siCHSB assessed by RT-qPCR. b siCHSB injection into third-instar reduces body length in fourth-instar larvae, as well as midgut length (c). d Percentage of pupation (x-axis) after egg-hatching. e Comparison of wing length in wild type (WT), negative control (NC) and siCHSB adults. f Number of follicles per ovary and the number of eggs per female mosquito (g) after injecting (n = 50) siCHSB. All surviving individuals were used for measurements, and results are shown as the mean ± SE (Student's t-tests: **P <0.01, ***P <0.001)
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