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2 results for “Cutaneotrichosporon oleaginosus”
Supplementary data for Model-driven engineering of Cutaneotrichosporon oleaginosus ATCC 20509 for improved microbial oil production
<p>Supplementary data corresponding to manuscript named Model-driven engineering of <em>Cutaneotrichosporon oleaginosus</em> ATCC 20509 for improved microbial oil production. </p> <p>The Supplementary material document contains supplementary figures and tables. The content of the figures and tables are indicated below. </p> <ul> <li>Figure S1. Plasmid map of pUC57NAT containing pGpd, nourseothricin acyltransferase gene and tGpd.</li> <li>Figure S2. Plasmid maps of overexpression targets containing TEF1α promoter, ATP-citrate lyase gene, TEF1α terminator, TPI1 promoter, Acetyl-CoA carboxylase gene, TPI1, YAT1 promoter, threonine synthase gene, YAT1 terminator and ENO1 promoter, hydroxymethylglutaryl-CoA synthase gene, ENO1 terminator.</li> <li>Table S2. Nucleotide sequences of promoters, genes, and terminators from <em>C. oleaginosus.</em></li> <li>Figure S3. Calibration curve of glycerol for calculating the glycerol concentration of medium.</li> <li>Figure S4. Volcano plots displaying differentially expressed genes and fold change (log2) in expression levels in WT, Δ9 and Δ12 strains at low lipid accumulation vs high lipid accumulation conditions.</li> <li>Figure S5. Flux distribution graphs of selected reactions for overexpression in C. oleaginosus.</li> <li>Figure S6. Colony PCR products were run on 1 % agarose gel. The colony PCR was performed for WT, ACL, ACC and TS transformants.</li> <li>Table S4. qPCR outputs, CT: The threshold cycle.</li> <li>Table S5. Fatty acid profile of C. oleaginosus grown at minimal medium with or without supplement (biotin, thiamine, threonine, serine, and aspartate) at 96h.</li> <li>Table S6. Lipid content, dry cell weight, and lipid weight of WT, ACL, ACC, TS, and HMGS <em>C. oleaginosus</em> at various C/N ratio minimal medium.</li> <li>Table S7. Fatty acid profile of WT, ACL, ACC, HMGS, and TS grown at C/N30, 120, 175, 200, and 300 minimal medium at 96h.</li> <li>Figure S7. Quadratic regression analysis on lipid accumulation, biomass and lipid content of wild-type, ACL, ACC, and TS C. oleaginosus at C/N 30, 120, 175, 200, 300.</li> <li>Table S8. Regression equations, statistics of regression equations for lipid content, biomass, and lipid content of wild-type, ACL, ACC, and TS.</li> <li>Table S9. Calculated optimum C/N ratios and responses (lipid content, biomass, and total lipid) by using built regression models for wild-type, ACL, ACC, and TS.</li> </ul> <p>Authors: </p> <p>Zeynep Efsun Duman-Özdamar<sup>a,b,c</sup>, Mattijs K. Julsing<sup>c</sup>, Janine A.C. Verbokkem<sup>c</sup>, Emil Wolbert<sup>c</sup>, Vitor A.P. Martins dos Santos<sup>a,b,d</sup>, Jeroen Hugenholtz<sup>e,f</sup>, Maria Suarez-Diez<sup>b*</sup></p> <p><sup>a</sup>Bioprocess Engineering, Wageningen University & Research, 6708 PB, Wageningen, the Netherlands</p> <p><sup>b</sup>Laboratory of Systems and Synthetic Biology, Wageningen University & Research, 6708 WE, Wageningen, the Netherlands</p> <p><sup>c</sup>Wageningen Food & Biobased Research, Wageningen University & Research, 6708 WE, Wageningen, The Netherlands</p> <p><sup>d</sup>LifeGlimmer GmbH, Berlin, 12163, Germany</p> <p><sup>e</sup>Faculty of Science Swammerdam Institute for Life Sciences, University of Amsterdam, 1090 GE Amsterdam, The Netherlands</p> <p><sup>f</sup>NoPalm Ingredients BV, 6709 PA Wageningen, The Netherlands</p>
Data deposition - Expanding the genetic toolbox for Cutaneotrichosporon oleaginosus employing newly identified promoters and two novel antibiotic resistance markers
<p>1. folder: Motif_discovery: Data used for motif discovery and results of the RSAT, MEME, and MAST tools</p> <p>2. folder: Plasmid_maps: Plasmid maps used and constructed in both SnapGene and GeneBank format.</p> <p>3. folder: Sanger_sequencing_genomic_integration: Sanger sequencing of the PCR products of the genomic DNA, each on the integrated gene or promoter to confirm genomic integration.</p>
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