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34 results for “DNA sequence alignment”
Multiple alignment of DNA-B sequences from CMMGV, EACMCV, EACMV, EACMKV, EACMMV, EACMZV, SACMV (7 "species")
<p>All sequences available in GenBank as of 2019-06-03 were downloaded via the Taxonomy Browser interface. Sequence names were normalized/simplified and orientations of these circular sequences were standardized to begin at the replication origin nick site. Sequences were aligned with MUSCLE and alignments were adjusted with SeAl (A. Rambaut) and AliView (A. Larsson).</p> <p>These results are described in a paper by Crespo-Bellido et al. (2021) https://doi.org/10.1128/JVI.00541-21</p>
Multiple alignment of ACMV and ACMBFV DNA-B sequences
<p>All sequences available in GenBank as of 2019-06-03 were downloaded via the Taxonomy Browser interface. Sequence names were normalized/simplified and orientations of these circular sequences were standardized to begin at the replication origin nick site. Sequences were aligned with MUSCLE and alignments were adjusted with SeAl (A. Rambaut) and AliView (A. Larsson).</p> <p>These results are described in a paper by Crespo-Bellido et al. (2021) https://doi.org/10.1128/JVI.00541-21</p>
Aligned DNA sequence matrix for phylogenetic analyses in the article "Three new species of Torrent Treefrogs (Anura: Hylidae) of the Hyloscirtus bogotensis group from the eastern Andean slopes and the biogeographic history of the genus"
<p>Aligned DNA sequence matrix for phylogenetic analyses of the article "Three new species of Torrent Treefrogs (Anura: Hylidae) of the Hyloscirtus bogotensis group from the Amazon foothills and the biogeographic history of the genus"</p> <p>The matrix is in NEXUS format and has 3259 bp and 25 terminals.</p> <p>Partitions are as follows:</p> <div>charset 12S = 1-955;</div> <div>charset ND1_nonCoding1 = 956-1279;</div> <div>charset ND1_Pos1 = 1280-2240\3;</div> <div>charset ND1_Pos2 = 1281-2241\3;</div> <div>charset ND1_Pos3 = 1282-2242\3;</div> <div>charset ND1_nonCoding2 = 2243-2361;</div> <div>charset cmyc_Pos1 = 2362-2779\3;</div> <div>charset cmyc_Pos2 = 2363-2780\3;</div> <div>charset cmyc_Pos3 = 2364-2781\3;</div> <div>charset Rag1_Pos1 = 2782-3415\3;</div> <div>charset Rag1_Pos2 = 2783-3416\3;</div> <div>charset Rag1_Pos3 = 2784-3417\3;</div>
Aligned DNA sequence matrix for phylogenetic analyses in the article "A new glassfrog of the genus Centrolene (Amphibia: Centrolenidae) from the Subandean Kutukú Cordillera, eastern Ecuador"
<p>Aligned DNA sequence matrix for phylogenetic analyses of the article "A new glassfrog of the genus Centrolene (Amphibia: Centrolenidae) from the Subandean Kutukú Cordillera, eastern Ecuador"</p> <p>The matrix is in NEXUS format and has 6626 bp and 239 terminals.</p> <p>Partitions are as follows:</p> <div> <div>charset 12S = 1-967;</div> <div>charset 16S = 968-2130;</div> <div> </div> <div>charset BNDFcodonPos1 = 2133-2829\3;</div> <div>charset BNDFcodonPos2 = 2131-2830\3;</div> <div>charset BNDFcodonPos3 = 2132-2828\3;</div> <div> </div> <div> </div> <div>charset ND1codonPos1 = 2832-3786\3;</div> <div>charset ND1codonPos2 = 2833-3787\3;</div> <div>charset ND1codonPos3 = 2831-3788\3;</div> <div> </div> <div> </div> <div>charset CXCR4codonPos1 = 3790-4144\3;</div> <div>charset CXCR4codonPos2 = 3791-4142\3;</div> <div>charset CXCR4codonPos3 = 3789-4143\3;</div> <div> </div> <div> </div> <div>charset cmyccodonPos1 = 4145-4547\3;</div> <div>charset cmyccodonPos2 = 4146-4548\3;</div> <div>charset cmyccodonPos3 = 4147-4549\3;</div> <div> </div> <div> </div> <div>charset POMCcodonPos1 = 4551-5160\3;</div> <div>charset POMCcodonPos2 = 4552-5161\3;</div> <div>charset POMCcodonPos3 = 4550-5162\3;</div> <div> </div> <div> </div> <div>charset RAG1codonPos1 = 5163-5616\3;</div> <div>charset RAG1codonPos2 = 5164-5617\3;</div> <div>charset RAG1codonPos3 = 5165-5618\3;</div> <div> </div> <div> </div> <div>charset SLC8A1codonPos1 = 5620-6160\3;</div> <div>charset SLC8A1codonPos2 = 5621-6158\3;</div> <div>charset SLC8A1codonPos3 = 5619-6159\3;</div> <div> </div> <div> </div> <div> </div> <div>charset SLC8A3codonPos1 = 6162-6627\3;</div> <div>charset SLC8A3codonPos2 = 6163-6625\3;</div> <div>charset SLC8A3codonPos3 = 6161-6626\3;</div> </div> <p> </p>
DNA sequences alignements for 27 species of ticks (SCO50 matrix)
<p>The two files contain respectively the concatenation of DNA sequences alignements for 27 species of ticks (SCO50 matrix, n=952 genes) and to the partition file indicating the positions of each gene in the concatenation.</p> <p>The data set corresponds to the article "A transcriptome-based phylogenetic study of hard ticks (Ixodidae)" to be published in Scientific Reports, by N Pierre Charrier, Axelle Hermouet, Caroline Hervet, Albert Agoulon,<br> Stephen Barker, Dieter Heylen, Céline Toty, Karen McCoy, Olivier Plantard, Claude Rispe.</p>
Aligned DNA sequence matrix for phylogenetic analyses in the article "New species of fossorial salamanders of the genus Oedipina (Plethodontidae) from the northwestern Ecuador"
<p>Aligned DNA sequence matrix for phylogenetic analyses of the article "New species of fossorial salamanders of the genus Oedipina (Plethodontidae) from the northwestern Ecuador". The matrix is in NEXUS format.</p> <p>Gene partitions are arranged as follows (tRNAs are included as part of larger adjacent genes):</p> <p>16S = 4- 789 1761- 1891 ;<br> ND1-codonPos1 = 790-1759\3;<br> ND1-codonPos2 = 791-1760\3;<br> ND1-codonPos3 = 792-1758\3;<br> CytB-codonPos1 = 1893-2274\3;<br> CytB-codonPos2 = 1894-2275\3;<br> CytB-codonPos3 = 1892-2276\3;</p>
Aligned and trimmed 16S and COI DNA sequences of Oceaniidae (Hydrozoa)
<p>Aligned and trimmed 16S and COI sequences of Oceaniidae (Hydrozoa) used for the study "The polyps of <em>Oceania armata</em> identified by DNA barcoding (Cnidaria, Hydrozoa)"</p> <p>Format is Fasta, files are text files</p>
The alignments of chloroplast genome sequences and nuclear ribosomal DNA fragments of six oak species sampled in the hot-dry valley of the Jinsha River, southwestern China
<p>Both chloroplast (cp) genome sequences and nuclear ribosomal (nr) DNA were assembled using GetOrganelle v.1.7.6.1 for 18 oak trees sampled in the Panzhihua Cycad National Nature Reserve, Sichuan Province, China. These trees belong to six oak species, including Quercus cocciferoides, Q. dolicholepis, Q. franchetii, Q. griffithii, Q. longispica, and Q. variabilis. We used PhyloSuite v.1.1.152 to extract coding sequences (CDSs), tRNA genes, rRNA genes, introns, and intergenic spacers (IGSs) of the 18 oak cp genomes. These sequences were aligned separately using MAFFT v.7.3.13 and manually adjusted with BioEdit v.7.2.5. Length variations in mononucleotide repeats were excluded and inversions were replaced with their reverse complements because of their tendency for homoplasy. Other indels were coded as binary characters according to the simple gap coding method using GapCoder. Separate assignments were concatenated according to their respective positions in the cp genome to obtain the alignments of LSC, SSC, IRb, and the whole cp genome.</p>
Aligned DNA sequence matrix for phylogenetic analyses in the article "Dos nuevas especies del grupo Pristimantis boulengeri (Anura: Strabomantidae) de la cuenca alta del río Napo, Ecuador" by Bejarano, et al.
<p>Matrix in nexus format that include sequences of 16S (1-1295), RAG1 (1296-1922), 12S (1923-3306), and COI (3307-3984) for 97 specimens belonging to the genus <em>Pristimantis</em>, in addition to specimens of <em>Strabomantis</em> and <em>Niceforonia</em> as outgroups.</p>
Aligned DNA sequence matrix for phylogenetic analyses of Scinax in the article "Advertisement calls and DNA sequences reveal a new species of Scinax (Anura: Hylidae) on the Pacific Lowlands of Ecuador"
<p>Aligned DNA sequence matrix for phylogenetic analyses of Scinax in the article "Advertisement calls and DNA sequences reveal a new species of Scinax (Anura: Hylidae) on the Pacific Lowlands of Ecuador". The matrix is in NEXUS format.</p> <p>Gene partition as arranged as follows (tRNAs are included as part of larger adjacent genes):</p> <p>12S RNA: 1-1033</p> <p>16S RNA: 1034-2832</p> <p>NADH dehydrogenase subunit 1: 2833-3793</p> <p>Cytochrome Oxidase sub-unit I: 3965-4654</p> <p>Cytochrome B: 4655-5447</p> <p> </p>
Aligned DNA sequence matrix for phylogenetic analyses of the article "A bizarre new species of Lynchius (Amphibia, Anura, Strabomantidae) from the Andes of Ecuador and first report of Lynchius parkeri in Ecuador"
<p>Aligned DNA sequence matrix for phylogenetic analyses of the article "A bizarre new species of <em>Lynchius</em> (Amphibia, Anura, Strabomantidae) from the Andes of Ecuador and first report of <em>Lynchius parkeri</em> in Ecuador"</p> <p>The matrix is in NEXUS format. Genes are arranged as follows:</p> <p>RAG1: 1-652<br> Tyrosinase: 653-1195<br> 12S RNA: 1196-2242<br> tRNA Val: 2243-2313<br> 16S RNA: 2314-3994<br> tRNA Leu = 3995-4065<br> ND1: 4066-5026<br> tRNA Ile: 5027-5144;</p>
Aligned DNA sequence matrix for phylogenetic analyses in the article "Systematics of Huicundomantis, a new subgenus of Pristimantis (Anura, Strabomantidae) with extraordinary cryptic diversity and eleven new species"
<p>Aligned DNA sequence matrix for phylogenetic analyses of the article "Systematics of Huicundomantis, a new subgenus of Pristimantis (Anura, Strabomantidae) with extraordinary cryptic diversity and eleven new species"</p> <p>The matrix is in NEXUS format. Genes are arranged as follows:</p> <p>16S RNA, tRNA-Leu = 1–1408<br> ND1 codon position 1 = 1409–2369\3;<br> ND1 codon position 2 = 1410–2367\3;<br> ND1 codon position 3 = 1411–2368\3;<br> tRNA-Ile, tRNA-Gln, rRNA-Met = 2370–2544; <br> RAG1 codon position 3 = 2545–3199\3;<br> RAG1 codon position 1 = 2546–3197\3<br> RAG1 codon position 2 = 2547–3198\3;</p>
Aligned DNA sequence matrixes for the study of the divergent times of phytoplasmas
<p>Sequence alignments of 16S rRNA and methionine aminopeptidase (map) are provided in FASTA files “Cao_et_al_16S.fas” and “Cao_et_al_map.fas”, respectively. Detailed information of the data matrixes is as follows:</p> <p> </p> <p>File name: Cao_et_al_16S.fas</p> <p>Number of taxa: 220</p> <p>Number of characters: 1655</p> <p>Gap: -</p> <p> </p> <p>File name: Cao_et_al_map.fas</p> <p>Number of taxa: 83</p> <p>Number of characters: 564</p> <p>Gap: -</p>
Aligned DNA sequence matrixes for the study of the divergent times of phytoplasmas
<p>Sequence alignments of 16S rRNA and methionine aminopeptidase (map) are provided in FASTA files “Cao_et_al_16S.fas” and “Cao_et_al_map.fas”, respectively. Detailed information of the data matrixes is as follows:</p> <p> </p> <p>File name: Cao_et_al_16S.fas</p> <p>Number of taxa: 220</p> <p>Number of characters: 1655</p> <p>Gap: -</p> <p> </p> <p>File name: Cao_et_al_map.fas</p> <p>Number of taxa: 83</p> <p>Number of characters: 564</p> <p>Gap: -</p>
Aligned DNA sequence matrix for phylogenetic analyses in the article "A new glassfrog of the genus Nymphargus (Anura: Centrolenidae) from Cordillera del Cóndor, Ecuador"
<p>Aligned DNA sequence matrix for phylogenetic analyses of the article "<span>A new glassfrog of the genus Nymphargus</span> (Anura<span>:</span> Centrolenidae) <span>from Cordillera del Cóndor, Ecuador</span>"</p> <p>The matrix is in NEXUS format and has 6613 bp and 102 terminals.</p> <p>Partitions are as follows:</p> <div> <div>charset 12S = 1-944;</div> <div>charset 16S = 945-2093;</div> <div>charset BNDFcodonPos1 = 2096-2792\3;</div> <div>charset BNDFcodonPos2 = 2094-2793\3;</div> <div>charset BNDFcodonPos3 = 2095-2791\3;</div> <div>charset ND1codonPos1 = 2795-3749\3;</div> <div>charset ND1codonPos2 = 2796-3750\3;</div> <div>charset ND1codonPos3 = 2794-3751\3;</div> <div>charset CXCR4codonPos1 = 3753-4107\3;</div> <div>charset CXCR4codonPos2 = 3754-4105\3;</div> <div>charset CXCR4codonPos3 = 3752-4106\3;</div> <div>charset cmyccodonPos1 = 4108-4507\3;</div> <div>charset cmyccodonPos2 = 4109-4508\3;</div> <div>charset cmyccodonPos3 = 4110-4509\3;</div> <div>charset POMCcodonPos1 = 4511-5120\3;</div> <div>charset POMCcodonPos2 = 4512-5121\3;</div> <div>charset POMCcodonPos3 = 4510-5122\3;</div> <div>charset RAG1codonPos1 = 5123-5576\3;</div> <div>charset RAG1codonPos2 = 5124-5577\3;</div> <div>charset RAG1codonPos3 = 5125-5578\3;</div> <div>charset SLC8A1codonPos1 = 5580-6120\3;</div> <div>charset SLC8A1codonPos2 = 5581-6118\3;</div> <div>charset SLC8A1codonPos3 = 5579-6119\3;</div> <div>charset SLC8A3codonPos1 = 6122-6587\3;</div> <div>charset SLC8A3codonPos2 = 6123-6585\3;</div> <div>charset SLC8A3codonPos3 = 6121-6586\3;</div> </div>
DNA sequences of transgenes detected via environmental DNA (raw ABI files, processed FASTA files, and reference alignments)
We demonstrate that simple, non-invasive environmental DNA (eDNA) methods can detect transgenes of genetically modified (GM) animals from terrestrial and aquatic sources in invertebrate and vertebrate systems. We detected transgenic fragments between 82-234 bp through targeted PCR amplification of environmental DNA extracted from food media of GM fruit flies (<i>Drosophila melanogaster</i>), feces, urine, and saliva of GM laboratory mice (<i>Mus musculus</i>), and aquarium water of GM tetra fish (<i>Gymnocorymbus ternetzi</i>). With rapidly growing accessibility of genome-editing technologies such as CRISPR, the prevalence and diversity of GM animals will increase dramatically. GM animals have already been released into the wild with more releases planned in the future. eDNA methods have the potential to address the critical need for sensitive, accurate, and cost-effective detection and monitoring of GM animals and their transgenes in nature.
Aligned DNA sequence matrix for phylogenetic analyses in the article "Our unknown neighbor: a new species of rain frog of the genus Pristimantis (Amphibia: Anura: Strabomantidae) from the city of Loja, southern Ecuador"
<p>The aligned matrix is in fasta format. Genes are arranged as follows:</p> <p>12S = 1–909</p> <p>16S = 910–1787</p> <p>RAG-1 = 1788–2399</p>
Aligned and curated mtDNA sequences from: Ancient DNA of narrow-headed voles reveals common features of the Late Pleistocene population dynamics in cold-adapted small mammals
<p><span>Narrow-headed vole, together with collared lemming and common vole, was the most abundant small mammal species across Eurasian Late Pleistocene steppe-tundra environments. Previous ancient DNA studies of </span><span>the latter</span><span> </span><span>two</span><span> revealed dynamic past population histories shaped by climatic fluctuations. To investigate the extent to which species with similar adaptations share common evolutionary </span><span>histories,</span><span> we generated a dataset comprising mitochondrial genomes of 139 ancient and 6 modern narrow-headed voles from multiple sites across Europe and north-</span><span>western</span><span> Asia and covering the last ca. 100 thousand years (ka). We inferred Bayesian time-aware phylogenies using 11 </span><span>radiocarbon-dated</span><span> samples for calibration of the molecular clock. We found that across the three </span><span>species,</span><span> divergence of the main mtDNA lineages occurred during Marine Isotope Stages (MIS) 7 and MIS 5, suggesting a common response </span><span>of species adapted to open habitat to the interglacial environments. </span><span>In European narrow-headed voles, we identified multiple </span><span>time-structured</span><span> mtDNA lineages, implying lineage turnovers. Timing of some of these turnovers was synchronous across all three </span><span>species,</span><span> allowing us to identify the main drivers of the Late Pleistocene dynamics of steppe- and cold-adapted species.</span></p>
Aligned DNA sequence matrix for phylogenetic analyses in the article "A new species of spiny-backed tree frog, genus Osteocephalus (Anura: Hylidae), from the Yanachaga Chemillén National Park in central Peru"
<p>Aligned DNA sequence matrix for phylogenetic analyses of the article "Systematics of Huicundomantis, a new subgenus of Pristimantis (Anura, Strabomantidae) with extraordinary cryptic diversity and eleven new species"</p> <p>The matrix is in NEXUS format and has 14791 bp and 38 terminals.</p> <p>Partitions are as follows:</p> <p>charset 12S_16S = 1-2442;<br> charset mtGenome_other_genes = 2443-9180; charset nonCoding = 3132- 3138 4776- 4857 5203- 5214;<br> charset codonPos1 = 2443-3130\3 3139-4774\3 4858-5200\3 5215-9178\3;<br> charset codonPos2 = 2444-3131\3 3140-4775\3 4859-5201\3 5216-9179\3;<br> charset codonPos3 = 2445-3129\3 3141-4773\3 4860-5202\3 5217-9180\3;<br> charset 16S_ND1nonCoding = 9181- 9428 10390- 10506 ;<br> charset 16S_ND1codonPos1 = 9429-10389\3;<br> charset 16S_ND1codonPos2 = 9430-10387\3;<br> charset 16S_ND1codonPos3 = 9431-10388\3;<br> charset POMCcodonPos1 = 10507-11068\3;<br> charset POMCcodonPos2 = 10508-11066\3;<br> charset POMCcodonPos3 = 10509-11067\3;<br> charset CO1codonPos1 = 11069-12608\3;<br> charset CO1codonPos2 = 11070-12609\3;<br> charset CO1codonPos3 = 11071-12610\3;<br> charset CytbcodonPos1 = 12611-13757\3;<br> charset CytbcodonPos2 = 12612-13758\3;<br> charset CytbcodonPos3 = 12613-13759\3;<br> charset ND2codonPos1 = 13760-14789\3;<br> charset ND2codonPos2 = 13761-14790\3;<br> charset ND2codonPos3 = 13762-14791\3;</p>
DNA sequences of transgenes detected via environmental DNA (raw ABI files, processed FASTA files, and reference alignments)
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