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152 results for “DNA viruses”
A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus
<p>Raw data associated to the study entitled:</p> <p><em>A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus</em><strong> </strong></p> <p><em>- </em>Metadata file</p> <p>- Computational data</p> <p>- Extraction data</p> <p>- Fluorescence detection</p> <p>- Fluorescence imaging</p> <p>- Labbooks</p> <p>- Surface characterization</p>
Data for publication: A pipeline for in-depth analysis of DNA virus populations by profiling the low abundant virus variants and partial genomic components
<p>Raw and processed sequence data from Oxford Nanopore and BGI short read sequencing platforms used in the publication: "A pipeline for in-depth analysis of DNA virus populations by profiling the low abundant virus variants and partial genomic components".</p>
Figure 6. DNA bands from T0 in Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus
Figure 6. DNA bands from T0 transgenic and Agro-infilterated plants. Lane 1-10, transgenic plants. Lane 11-13, agro-infilterated plants.
A plant virus differentially alters DNA methylation in two cryptic species of a hemipteran vector
<p>This study investigated DNA methylation patterns in two cryptic species (B and Q) of the sweet potato whitefly, <em>Bemisia tabaci</em> (Gennadius), following the acquisition of the tomato yellow curl virus, a single-stranded DNA virus. The methylation levels in genomic features such as promoters, gene bodies, and transposable elements in both cryptic species were described in this study. While overall trends were found to be similar, specific differences in methylation levels were observed. Virus-induced differentially methylated regions (DMRs) were associated with different genes in each cryptic species and were negatively correlated with differential gene expression. These DMRs were analyzed for changes in gene expression and alternative splicing, revealing clusters of hyper- and hypomethylated genes related to virus-vector interactions, immune functions, and detoxification processes. These methylation differences may help explain the distinct biological and physiological traits observed between the B and Q cryptic species.</p>
A plant virus differentially alters DNA methylation in two cryptic species of a hemipteran vector
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Wide spectrum and high frequency of genomic structural variation, including transposable elements, in large double stranded DNA viruses
Our knowledge of the diversity and frequency of genomic structural variation segregating in populations of large double stranded (ds) DNA viruses is limited. Here we sequenced the genome of a baculovirus (AcMNPV) purified from beet armyworm (Spodoptera exigua) larvae at depths >195,000X using both short-read (Illumina) and long-read (PacBio) technologies. Using a pipeline relying on hierarchical clustering of structural variants (SVs) detected in individual short- and long-reads by six variant callers, we identified a total of 1,141 SVs in AcMNPV, including 464 deletions, 443 inversions, 160 duplications and 74 insertions. These variants are considered robust and unlikely to result from technical artifacts because they were independently detected in at least three long reads as well as at least three short reads. SVs are distributed along the entire AcMNPV genome and may involve large genomic regions (30,496 bp on average). We show that no less than 39.9% of genomes carry at least one SV in AcMNPV populations, that the vast majority of SVs (75%) segregate at very low frequency (<0.01%) and that very few SVs persist after 10 replication cycles, consistent with a negative impact of most SVs on AcMNPV fitness. Using short-read sequencing datasets, we then show that populations of two iridoviruses and one herpesvirus are also full of SVs, as they contain between 426 and 1102 SVs carried by 52.4 to 80.1% of genomes. Finally, AcMNPV long reads allowed us to identify 1,757 transposable elements (TEs) insertions, 895 of which are truncated and occur at one extremity of the reads. This further supports the role of baculoviruses as possible vectors of horizontal transfer of TEs. Altogether, we found that SVs, which evolve mostly under rapid dynamics of gain and loss in viral populations, represent an important feature in the biology of large dsDNA viruses.
reference proteins for large DNA viruses
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Truly ubiquitous CRESS DNA viruses scattered across the eukaryotic tree of life
<p>Until recently, most viruses detected and characterized were of economic significance, associated with agricultural and medical diseases. This was certainly true for the eukaryote-infecting circular Rep (replication-associated protein)-encoding single-stranded DNA (CRESS DNA) viruses, which were thought to be a relatively small group of viruses. With the explosion of metagenomic sequencing over the past decade and increasing use of rolling-circle replication for sequence amplification, scientists have identified and annotated copious numbers of novel CRESS DNA viruses – many without known hosts but which have been found in association with eukaryotes. Similar advances in cellular genomics have revealed that many eukaryotes have endogenous sequences homologous to viral Reps, which not only provide "fossil records" to reconstruct the evolutionary history of CRESS DNA viruses but also reveal potential host species for viruses known by their sequences alone. The Rep protein is a conserved protein that all CRESS DNA viruses use to assist rolling circle replication that is known to be endogenized in a few eukaryotic species (notably tobacco and water yam). A systematic search for endogenous Rep-like sequences in GenBank's non-redundant eukaryotic database was performed using tBLASTn. We utilized relaxed search criteria for the capture of integrated Rep sequence within eukaryotic genomes, identifying 93 unique species with an endogenized fragment of Rep in their nuclear (78 species), plasmid (1 species), mitochondrial (6 species) or chloroplast (8 species) genomes. These species come from 19 different phyla, scattered across the eukaryotic tree of life. Exogenous and endogenous CRESS DNA viral Rep tree topology suggested potential hosts for one family of uncharacterized viruses and supports a primarily fungal host range for genomoviruses.</p>
A longitudinal study of DNA and RNA viruses plasma detection in allogeneic hematopoietic stem cell transplant recipients
<p><span><strong>Background:</strong> </span><span>Viral infections are among the most common complications after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and can be associated with transient or sustained viremia. Besides viruses that are common causes of infection, metagenomics revealed the presence of several novel viruses and variants that are overlooked in clinical routine and represent potential sources of unrecognized systemic infections</span><span>. Our aim was to describe the prevalence and the dynamics of 17 DNA and 3 RNA viral infections using (r(RT-)PCR) assays on plasma samples of adult allo-HSCT recipients over a one-year period after HSCT.</span></p> <p><strong><span>Methods:</span></strong><span> 109 adult patients that received a first allo-HSCT from 1<sup>st</sup> March 2017 to 31<sup>st</sup> January 2019 we included in this</span> <span>longitudinal observational monocentric cohort study</span><span>.</span> <span>17 DNA and 3 RNA viral species were screened with qualitative and/or quantitative r(RT)-PCR assays performed on plasma samples </span><span>collected at five time-points (day 0 and 30 days, 3 months, 6 months and one year after HSCT). </span></p> <p><strong><span>Results: </span></strong><span>TTV was the most prevalent with an increasing prevalence to 96% of patients at 3 months. HPgV-1 prevalence ranged from 26 to 36% of patients. TTV and HPgV-1 plasma viral load peaked at month 3 (TTV: median 3.29E5 copies/ml [range, 3.37E2 to 4.06E9 copies/ml]; HPgV-1: median 1.18E6 copies/ml [range, 2.61E3 to 4.49E7 copies/ml]). Among <em>Polyomaviridae</em>, BKPyV, JCPyV, MCPyV, HPyV6 and 7 were detected in ≥10% of patients at ≥1 time-point. HPyV6 and HPyV7 prevalence reached 27% and 12% of patients at month 3. Among those, 41% and 63% had quantifiable viral loads, with median viral loads above 1E3copies/ml and results may suggest HPyV6 sustained viremia. Co-detections were frequent, in particular at 3 months with ≥2 viruses detected in 72% of patients. </span></p> <p><strong><span>Conclusion: </span></strong><span>Our study confirms that TTV and HPgV-1 infections are highly prevalent and that infection may be sustained up to one year after allo-HSCT. Our systematic and large strategy of screening also revealed diverse and numerous co-detections, and that several novel <em>Polyomaviridae</em> (MCPyV, HPyV6/7) that are overlooked in clinical routine are as or more frequently detected compared to classical culprits. Our results underscores the need for further studies investigating the clinical impact of classical culprits together with other viruses in particular novel <em>Polyomaviridae</em> and HPgV-1. </span></p>
All-atom models of SU10 and P68 viral genomes. Supplementary to the "Are kuravirus capsid diameters quantized? The first all-atom genome tracing method for double-stranded DNA viruses".
<p>The dataset contains mmCIF formatted all-atom models of SU10 and P68 viral genomes and the python script used for refinement of the initial MMB-generated coordinates.</p>
Andes Virus DNA Vaccine for the Prevention of Hantavirus Pulmonary Syndrome Using the PharmaJet Stratis(R) Needle-Free Injection Delivery Device
ClinicalTrials.gov study NCT03682107. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Safety and Immunogenicity Study of a Dengue Virus DNA Vaccine
ClinicalTrials.gov study NCT00290147. IPD Sharing: NO. Countries: 1. Publications: 2.
Study to Assess Brincidofovir Treatment of Serious Diseases or Conditions Caused by Double-stranded DNA Viruses
ClinicalTrials.gov study NCT01143181. IPD Sharing: Not stated. Countries: 1. Publications: 1.
VRC 705: A Zika Virus DNA Vaccine in Healthy Adults and Adolescents
ClinicalTrials.gov study NCT03110770. IPD Sharing: NO. Countries: 9. Publications: 4.
Truly ubiquitous CRESS DNA viruses scattered across the eukaryotic tree of life
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A longitudinal study of DNA and RNA viruses plasma detection in allogeneic hematopoietic stem cell transplant recipients
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Wide spectrum and high frequency of genomic structural variation, including transposable elements, in large double stranded DNA viruses
Open the record for dataset details and reuse information.
Recurrent evolution of two competing haplotypes in an insect DNA virus
<p>Hosts and viruses are constantly evolving in response to each other: as hosts attempt to suppress the virus, the virus attempts to evade and suppress the host's immune system. This arms race results in the evolution of novel pathways in both the host and virus to gain the upper hand. Here we describe the coevolution between <i>Drosophila </i>species and a common and virulent DNA virus. We identify two distinct viral types that differ 100-fold in viral titer in infected individuals, with similar effects across multiple species. Our analysis suggests that one of the viral types appears to have recurrently evolved at least 4 times in the past ~30,000 years, including in another geographically distinct species, due to the high effective mutation rate which increases with titer. The higher titer viral type is associated with suppression of the host immune system and an increased transmission rate compared to the low viral titer type. Both types are maintained in all populations, likely due to an increased virulence in the high titer type creating a trade-off between effective transmission and virulence and resulting in nearly equal reproduction rates (R<sub>0</sub>) in both types. Together these results suggest that the reciprocal selective pressures caused by the co-evolution between host and virus has resulted in this recurrently evolving relationship.</p>
Data from: Distinct spread of DNA and RNA viruses among mammals amid prominent role of domestic species
<p><b>Aim:</b> Emerging infectious diseases arising from pathogen spillover from mammals to humans comprise a substantial health threat. Tracing virus origin and predicting the most likely host species for future spillover events are major objectives in One Health disciplines.</p> <p>We assessed patterns of virus sharing among a large diversity of mammals, including humans and domestic species.</p> <p><b>Location:</b> Global.</p> <p><b>Time period:</b> Current.</p> <p><b>Major taxa studied:</b> Mammals and associated viruses.</p> <p><b>Methods:</b> We used network centrality analysis and trait-based Bayesian hierarchical models to explore patterns of virus sharing among mammals. We analysed a global database that compiled the associations between 1,785 virus species and 725 mammalian host species as sourced from automatic screening of meta-data accompanying published nucleotide sequences between 1950 – 2019.</p> <p><b>Results:</b> We show that based on current evidence, domesticated mammals hold the most central positions in networks of known mammal-virus associations. Among entire host-virus networks, Carnivora and Chiroptera hold central positions for mainly sharing RNA viruses, while Ungulates hold central positions for sharing both RNA and DNA viruses with other host species. We revealed strong evidence that DNA viruses were phylogenetically more host specific than RNA viruses. RNA viruses exhibited low functional host specificity despite an overall tendency to infect phylogenetically related species, signifying high potential to shift across hosts with different ecological niches. The frequencies of sharing viruses among hosts and the proportion of zoonotic viruses in hosts were larger for RNA than DNA viruses.</p> <p><b>Main conclusions:</b> Acknowledging the role of domestic species in addition to host and virus traits in patterns of virus sharing is necessary to improve our understanding of virus spread and spillover in times of global change. Understanding multi-host virus sharing pathways adds focus to curtail disease spread.</p>
FIGURE 3 in First record of the West Nile virus bridge vector Culex modestus Ficalbi (Diptera Culicidae) in Belgium, validated by DNA barcoding
FIGURE 3. NJ tree based on COI sequences downloaded from BOLD, including Culex species recorded from Belgium (Boukraa et al. 2015) and sequences of Cx. modestus (Table 1). Bootstrap values are indicated above the branches. The blue square gives a zoom view of the un-collapsed tree.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.