Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
45
datasets available to search
ShareScore release 0.9.0
Dataset results
45 results for “Diabrotica”
Dataset of the paper entitled methods for high-throughput screening of novel agents against the maize pest, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)
<p>Title: Methods for high-throughput screening of novel agents against the maize pest, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) </p> <p>Authors: Sri Ita Tarigan, Gyorgy Turoczi, Jozsef Kiss, Stefan Toepfer</p> <p>Abstract: <br>The western corn rootworm, <em>Diabrotica virgifera virgifera</em> (Coleoptera: Chrysomelidae), poses a significant threat to maize crops in North America and Europe, necessitating development of novel, effective, and less disruptive crop protection agents. With recent bans on key insecticides and concerns about overuse of remaining options, there is an urgent need for accessible and comparable screening methods. We propose comparative high-throughput screening methods against the eggs, larvae and adults of this pest, emphasizing the importance of suitable positive controls tailored to the specific bioassay types. We evaluated seven common insecticides (imidacloprid, clothianidin, acetamiprid, novaluron, cypermethrin, chlorpyrifos-methyl, spinosad) against eggs, larvae, and adults as potential positive controls for each of the proposed assay methods. Dipping assays with ready-to-hatch eggs revealed several ingredients to cause mortality; but imidacloprid might be most suitable as a positive control due to its robust dose-response in reducing egg hatching and causing mortality of hatching neonates. Larval bioassays using artificial diet overlay assays revealed mortality caused by all insecticides, with imidacloprid and acetamiprid exhibiting best dose-mortality response curves as well as sublethal effects. Adult bioassays using artificial diet-core overlay assays revealed mortality caused by all insecticides, with cypermethrin or acetamiprid exhibiting best dose-mortality response curves. The provided ED <sub>50</sub>, ED <sub>80</sub> values, and dose-response equations offer valuable insight for researchers in selecting appropriate positive controls for screening new crop protection agents or assessing resistance levels against different life stages of this pest.</p> <p>Data:</p> <p>The data file is related to the screening of commercial insecticides against eggs, first instar larvae (L1) and adults of the maize pest, <em>Diabrotica virgifera virgifera</em> using standard bioassays. We are proposing comparative high-throughput screening methods against the eggs, larvae and adults of this maize pest. This includes the crucial aspect of suitable positive controls tailored to the specific bioassay type. We evaluated seven common insecticides (imidacloprid, clothianidin, acetamiprid, novaluron, cypermethrin, chlorpyrifos-methyl, spinosad) against eggs, larvae, and adults as potential positive controls for each of the proposed assay method. To access effects and dose-responses of commonly used insecticides on eggs, we applied standard screening methods under controlled semi-sterile conditions.</p> <p>For egg bioassays, eggs were transferred to the 200 ml of treatments in the eppendorf tubes and then soaked for 1 hour. Then 20µl with 10 to 20 eggs were pipetted onto a filter paper in a petri dish (150 mm×25 mm). Then 100 µl of sterilized tap water was added for moisture. The pipette tip was replaced between treatments. The eggs been transferred were counted per filter paper and dish (15± 8). The eggs were then incubated in the dishes at 23-25<sup>0</sup>C for 7 days, when the experiment was terminated. Egg hatching, mortality of newly hatching larvae, and days until start of egg hatching were observed under stereo microscope and recorded. Data were collected at 1,3, 5 and 7 days after treatments.</p> <p>To assess the effect and dose-responses of commonly used insecticides on neonates of <em>D. v. virgifera</em>, we applied artificial diet-overlay bioassays under controlled semi-sterile conditions. Each insecticide was prepared in at least six concentrations. Each bioassay consisted of 3 to 6 polystyrene plates of 96 wells each (07-6096 of Biologix Ltd., USA, or Costar 3917 of Corning Inc., USA). Each well had a volume of 330 µl, with a diameter of 5 mm, a height of 10 mm, and a surface area of 0.34 cm². 190 µl of the diet were pipetted into each 330 µl well, filling each to approximately 2/3<sup>rd </sup>of its capacity. Plates containing the diet were left to dry in a laminar flow cabinet for 45 minutes and then stored overnight at temperatures ranging from 3 to 5°C. The following day, treatments were applied. This is, 17 µl of a treatment was applied to the 0.34 cm<sup>2</sup> diet surface reaching good coverage and therefore forcing the after-placed larvae to feed through (10 to 100 µl pipette Biohit TM Proline). Each treatment was applied to 8 wells per plate. Following application, the plates were allowed to dry for a duration of 1 to 1.5 hours and were subsequently cooled for 1 hour in a refrigerator set at temperatures between 23 to 25°C. Each well received one neonate larva, carefully placed on the diet surface using a fine artist brush. A vigorous and visibly healthy larva was selected, lifted from the end of the abdomen with the brush, maneuvered towards a well surface, and allowed to crawl off the brush onto the diet. To avoid systematic errors, larvae were not arranged in treatment column order but rather in a rectangular pattern. After every 12 individual larvae, the brush was cleaned using 70% ethanol followed by sterile tap water. The filled plate was sealed with an optically clear adhesive qPCR seal sheet (#AB-1170, Termo Scientific, USA, or #BS3017000, Bioleader, USA), enabling data assessments without the need to open the plate. Four to five holes were carefully made with fine 00-insect pins into the seal per well to facilitate aeration. The plates, housing the larvae, were then incubated in a dark, ventilated incubator at a temperature of 23-25 °C and a relative humidity of 50 to 90% for a period of 5 days. We assessed mortality and stunting larvae within 3 and 5 days. </p> <p>To access the effect and dose-responses of common insecticides on <em>D.v.virgifera</em> adults, artificial diet-overlay bioassays with different doses were performed under controlled, semi-sterile conditions. Each insecticide was prepared in at least six concentrations. Active ingredients as specified on the product labels underwent serial dilutions using sterile tap water. Sterilized tap water was used as untreated control. In detail, each bioassay consisted of 6 polystyrene plates of 6 wells each (Eppendorf® 0030720016). Each treatment was applied to 3 wells of each plate per bioassay. The adult diet for a bioassay had been prepared 1-7days before treatment and adult infestation. The diet was prepared under semi-sterile conditions. The diet was poured out to 5-6 sterile 11 mm Petri dishes. The plates with diet were allowed to dry for up to 15 minutes under laminar flow cabinet then stored at 3 to 5°C overnight.The following day, a core of the diet was initially transferred to each well using flamed iron core-cutter (1 cm diameter) under a laminar flow. A core diet was placed each of the 6 wells of the plates. Approximately 40 µl of the treatments were then applied across the surface of diet core (0.34 cm<sup>3</sup>). The following day, a core of the diet was initially transferred to each well using flamed iron core-cutter (1 cm diameter) under a laminar flow. A core diet was placed each of the 6 wells of the plates. Approximately 40 µl of the treatments were then applied across the surface of diet core (0.34 cm<sup>3</sup>). Adult were subsequently transferred from the rearing cage into the wells of the 6-well plates containing the diet and treatments using a tube aspirator. For ease of transfer, the adults were cooled in a fridge for 4 to 7 minutes. Each well plate received 3 to 4 adults. Plates were sealed and incubated at 23-25<sup>0</sup>C, 50–90% r.h, L: D 12:12. Adult mortality were recorded on days 1,3, 5, 7 of experiment. </p> <p>To allow comparisons between experiments, data were standardized to the data of the corresponding negative control, usually sterilized tap water, as follows: standardized data = 100 × (data in negative control - data in treatment)/maximum (data in control or in treatment). The distributions of the data were investigated using histograms and QQ normal and detrended normal probability. Skewness and kurtosis of residuals was also observed for normality of influences of treatments on eggs, neonates, or adults. Equality of variances was assessed using Levene’s test. Multiple comparisons were performed using the Tukey HSD post hoc test for data with equal variances and the Games-Howell post hoc test for data with unequal variances. For each tested insecticide, linear and logarithmic regression models were fit to the dose-response data. In case of significant linear or logartimic relathionships, doses leading to 50% or 80% of relative effects (ED <sub>50,80</sub>) were calculated. </p> <p>The raw data as well as the standardised data are available as a csv file on zenodo. </p> <p> </p> <p> </p>
Can the botanical azadirachtin replace phased-out soil insecticides in suppressing the soil insect pest Diabrotica virgifera virgifera ?
<p><strong>Can the botanical <em>azadirachtin</em> replace phased-out soil insecticides in suppressing the soil insect pest <em>Diabrotica virgifera virgifera </em>?</strong></p> <p><strong>Background</strong></p> <p>Due to recent bans on the use of several soil insecticides and insecticidal seed coatings, soil-dwelling insect pests are increasingly difficult to manage. One example is the western corn rootworm (<em>Diabrotica virgifera virgifera</em>, Coleoptera: Chrysomelidae), a serious root-feeder of maize (<em>Zea mays</em>). We investigated whether the less problematic botanical <em>azadirachtin</em>, widely used against above-ground insects, could become an option for the control of this soil insect pest.</p> <p><strong>Methods</strong></p> <p>Artificial diet-based bioassays were implemented under standard laboratory conditions to establish lethal dose curves for the pest larvae. Then, potted-plant experiments were implemented in greenhouse to assess feasibility and efficacy of a novel granular formulation of <em>azadirachtin </em>under more natural conditions and in relation to standard insecticides.</p> <p><strong>Results</strong></p> <p>Bioassays in three repetitions revealed a 3-day LD<sub>50</sub> of 22.3 µg <em>azadirachtin</em> per ml which corresponded to 0.45 µg per neonate of <em>D. v. virgifera </em>and a 5-day LD<sub>50</sub> of 19.3 µg per ml or 0.39 µg per first to second instar larva. No sublethal effects were observed. The three greenhouse experiments revealed that the currently proposed standard dose of a granular formulation of 38 g<em> azadirachtin </em>per hectare for in-furrow application at sowing is not enough to control <em>D. v. virgifera </em>or to prevent root damage. At 10x standard-dose total pest control was achieved as well as the prevention of most root damage. This was better than the efficacy achieved by <em>cypermethrin</em>-based granules and comparable to <em>tefluthrin</em>- granules, or <em>thiamethoxam</em> seed coatings. The ED<sub>50</sub> for suppressing larval populations were estimated at 92 g <em>azadirachtin</em> per ha, for preventing heavy root damage 52 g /ha and for preventing general root damage 220 g /ha.</p> <p><strong>Conclusions</strong></p> <p>There seems clear potential for the development of neem-based botanical soil insecticides for arable crops such as maize. They might become, if doses are increased and more soil insecticides phased out, a promising, safer solution as part of the integrated pest management toolkit against soil insects.</p>
Fig. 3 in Antixenosis and tolerance to Diabrotica speciosa (Coleoptera: Chrysomelidae) in common bean cultivars
Fig. 3. Dendrogram based on plant growth parameters recorded in common bean cultivars infested by Diabrotica speciosa larvae. The arrow indicates the Euclidian distance used for group separation.
Fig. 5 in Antixenosis and tolerance to Diabrotica speciosa (Coleoptera: Chrysomelidae) in common bean cultivars
Fig. 5. Analysis of Pearson's simple linear correlation between reduction percentage of the dry weight of the plant root system and dry weight of the plant aerial part in common bean cultivars infested by Diabrotica speciosa larvae.
Fig. 4 in Antixenosis and tolerance to Diabrotica speciosa (Coleoptera: Chrysomelidae) in common bean cultivars
Fig. 4. Distribution of plant growth parameters and common bean cultivars following principal component analysis obtained from plants infested by Diabrotica speciosa larvae.
Fig. 2 in Antixenosis and tolerance to Diabrotica speciosa (Coleoptera: Chrysomelidae) in common bean cultivars
Fig. 2. Feeding preference index of Diabrotica speciosa adults to common bean cultivars in the no-choice test.
Fig. 1 in Antixenosis and tolerance to Diabrotica speciosa (Coleoptera: Chrysomelidae) in common bean cultivars
Fig. 1. Feeding preference index of Diabrotica speciosa adults to common bean cultivars in the free-choice test.
Fig. 2 in Sexual dimorphism in Diabrotica speciosa and Diabrotica viridula (Coleoptera: Chrysomelidae)
Fig. 2. Prothoracic, mesothoracic and metathoracic legs of male and female of Diabrotica speciosa, showing the sexual dimorphism in the basal tarsomere of the pro- and mesothoracic legs. Setae: D, discoid; F, filamentous; L, lanceolate; S, spatulate.
Fig. 1 in Sexual dimorphism in Diabrotica speciosa and Diabrotica viridula (Coleoptera: Chrysomelidae)
Fig. 1. Prothoracic, mesothoracic and metathoracic legs of male and female of Diabrotica viridula, showing the sexual dimorphism in the basal tarsomere of the pro- and mesothoracic legs. Setae: D, discoid; F, filamentous; L, lanceolate; S, spatulate.
On understanding and manipulating the hatching patterns of Diabrotica v. virgifera (Coleoptera: Chrysomelidae) eggs to improve the design of experiments
<p><em>Diabrotica v. virgifera </em>(Coleoptera: Chrysomelidae) is a well-studied pest of maize in North America and Europe. Many studies on its biology, behaviour or management rely individuals reared from either field-collected insect or on laboratory colonies. Naturally, <em>D. v. virgifera</em> eggs require an obligate diapause which can cause technical challenges such as a deceleration of research activities. To allow better planning of experimentation, we have investigated the survival and temporal hatching patterns of the pest`s eggs depending on diapause length as well as on post-diapause incubation temperature. Several series of laboratory assays revealed that eggs from diapausing populations hatch at highest rates and with most synchronized hatching start and peaks when overwintered at natural diapause length of eight to ten months or shorter down to five months (20 to 24 °C incubation temperatures). Comparably good hatching rates were found in eggs diapaused for only two months, but hatching patterns appeared more spread and variable. Diapause shorter than two months or longer than ten months reduced hatching successes, as did low incubation temperatures. Data matrices on egg overwintering survival, on the beginning, peak, duration and termination of egg hatching, as well as on hatching rates are provided for different diapause lengths and incubation temperatures of diapausing and, as a comparison, non-diapausing <em>D. v. virigifera</em> to support scientists choosing a colony which fits best their experimental setup and study conditions, as well as to properly plan such studies.</p>
FIGURE 27 in Morphological and molecular characterization of a new species of Diabrotica (Coleoptera, Chrysomelidae, Galerucinae)
FIGURE 27. Cladogram of the strict consensus tree based on the combined data of COI gene and ITS-1 nuclear region generated by Maximum-Parsimony. Bootstrap percentages of 1000 replicates are shown above the branches. Morphological species group are shown to illustrate taxonomic congruence. Only values of 50% or greater are shown.
FIGURE 26 in Morphological and molecular characterization of a new species of Diabrotica (Coleoptera, Chrysomelidae, Galerucinae)
FIGURE 26. Cladogram of the strict consensus tree based on COI mitochondrial data (452 characters) generated by parsimony analysis as implemented Phylogenetic Analysis Using Parsimony (PAUP 4.0). Bootstrap analysis of 1000 replicates was performed on the data using Parsimony method using the heuristic search algorithm. Numbers shown on the branches are bootstrap percentages derived from parsimony. Only values of 50% or greater are shown.
FIGURES 2–9 in Morphological and molecular characterization of a new species of Diabrotica (Coleoptera, Chrysomelidae, Galerucinae)
FIGURES 2–9. Diabrotica wartensis Cabrera & Sosa-Gómez (2) Labrum, dorsal view. (3) Maxilla, ventral view. (4) Metanotum. (5) Metendosternite, dorsal view. (6) Hind wing. (7). Median lobe, lateral view. (8) Median lobe, detail of apex and internal sac, dorsal view. (9) Female genitalia. Abbreviations: a, metanotal ridge a; AA, anal anterior vein; b1, metanotal ridge b1; b2; c, metanotal ridge c; CuA, cubitoanal vein; CuA 3+4, cubitoanal vein 3+4; d, metanotal ridge d; mg, median groove; MP 1-2, medial posterior vein 1-2; os, ostium; pu, pump; RA, radial vein; RAc, radial cell; re, receptacle; RP-MP2, radial posterior-medial posterior vein 2; SC, subcostal vein; sc1, sclerite 1; sc2, sclerite 2; sc3, sclerite 3; sc4, sclerite 4; ssd, sclerotized spermathecal duct; tg, tignum; tl, triangular lobe; va-bu, vagina-bursa copulatrix; vg, vaginal palpi. Scale bars= 0.1mm.
FIGURE 25 in Morphological and molecular characterization of a new species of Diabrotica (Coleoptera, Chrysomelidae, Galerucinae)
FIGURE 25. Cladogram of the strict consensus tree based on the ITS-1 nuclear region generated by parsimony analysis as implemented Phylogenetic Analysis Using Parsimony (PAUP 4.0) and heuristic search algorithm. Bootstrap analysis of 1000 replicates was performed on the data using Parsimony method. Numbers shown on the branches are bootstrap percentages derived from parsimony. Only values of 50% or greater are shown.
FIGURES 10–15 in Morphological and molecular characterization of a new species of Diabrotica (Coleoptera, Chrysomelidae, Galerucinae)
FIGURES 10–15. Diabrotica wartensis Cabrera & Sosa-Gómez (10) Head, frontal view. (11) Head, lateral view. (12) Mandible, external face. (13) Maxilla, dorsal view. (14) Maxilla, detail of digitiform sensillum. (15) Labium, ventral view. Abbreviations: ac, antennal callus; afr, anterofrontal ridge; bs, basistipes; dgs, digitiform sensillum; ds, ditistipes; fr, frontal ridge; ga, galea; lc, lacinia; lg, ligula; mo, mola; mp, maxillary palp; prm, prementon; sm, setose membrane; th3, tooth 3; th4, tooth 4; th5, tooth 5.
FIGURES 13−16 in Diabrotica collicola (Coleoptera: Chrysomelidae), a new species of leaf beetle from Argentina and key to species of the Diabrotica virgifera group and relatives
FIGURES 13−16. Diabrotica collicola Cabrera & Cabrera Walsh (13) Elytron, detail of binding patch and submarginal ribbon. (14) (E) Detail of anterior area of binding patch, surface covered with stump-shaped spicules and sharktoothshaped spicules. (15) Detail of posterior area of binding patch covered with sharktooth-shaped spicules (16) Detail of submarginal ribbon along the surface near anterior margin, covered with thin microspicules. Abbreviations: bp, binding patch; shs, sharktooth spicules; sr, submarginal ribbon; sts, stump spicules.
FIGURES 17−19 in Diabrotica collicola (Coleoptera: Chrysomelidae), a new species of leaf beetle from Argentina and key to species of the Diabrotica virgifera group and relatives
FIGURES 17−19. Diabrotica collicola Cabrera & Cabrera Walsh (17) Median lobe, lateral view. (18) Median lobe, detail of apex and internal sac, dorsal view. (19) Female genitalia. Scale bars= 0.1 mm. Abbreviations: bf, basal foramen; os, ostium; pu, pump; re, receptacle; sc1, sclerite 1; sc2, sclerite 2; sc3, sclerite 3; sc4, sclerite 4; ssd, sclerotized spermathecal duct; tg, tignum; tl, triangular lobe; va-bu, vagina-bursa copulatrix; vg, vaginal palpi.
FIGURES 9−12 in Diabrotica collicola (Coleoptera: Chrysomelidae), a new species of leaf beetle from Argentina and key to species of the Diabrotica virgifera group and relatives
FIGURES 9−12. Diabrotica collicola Cabrera & Cabrera Walsh (9) Maxilla, detail of digitiform sensillum. (10) Metendosternite, dorsal view. (11) Median lobe, lateral view. (12) Median lobe, dorsal view. Abbreviations: bf, basal foramen; ds, digitiform sensillum; la, lateral arms; op, orificial plate; os, ostium; st, stalk.
FIGURES 2−8 in Diabrotica collicola (Coleoptera: Chrysomelidae), a new species of leaf beetle from Argentina and key to species of the Diabrotica virgifera group and relatives
FIGURES 2−8. Diabrotica collicola Cabrera & Cabrera Walsh (2) Labrum, dorsal view. (3) Labrum, ventral view. (4) Mandible, external face. (5) Maxilla, ventral view. (6) Hind wing. (7) Metendosternite, ventral view. (8) Metanotum. Scale bars= 2−5, 7: 0.1mm; 6, 8: 1 mm. Abbreviations: a, metanotal ridge a; AA, anal anterior vein; b2, metanotal ridge b2; bs, basistipes; c, metanotal ridge c; CuA, cubitoanal vein; CuA 3+4, cubito anal vein 3+4; d, metanotal ridge d; ds, ditistipes; ga, galea; lc, lacinia; mg, median groove; mo, mola; MP 1−2, medial posterior vein 1−2; RA, radial vein; RP, radial posterior vein; re, receptacle; RP-MP2, radial posterior-medial posterior vein 2; SC, subcostal vein; sm, setose membrane; th3, tooth 3; th4, tooth 4; th5, tooth 5.
FIGURES 75–79. Diabrotica fasciata Kirsch. 75 in Taxonomic changes in the genus Diabrotica Chevrolat (Coleoptera: Chrysomelidae: Galerucinae): results of a synopsis of North and Central America Diabrotica species
FIGURES 75–79. Diabrotica fasciata Kirsch. 75—dorsal view, lectotype, 76—lateral view, lectotype, 77—internal sac of the aedeagus, ventral view, 78—lateral view, left, 79—lateral view, right.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.