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Dataset results
29 results for “Downy mildew”
Trinity assembly of Downy mildew of Grapevine lesion samples DMG-C
<p>De novo Trinity assembly from RNAseq</p>
Pangenome graph analysis reveals extensive effector copy-number variation in spinach downy mildew
<p>Data produced for the comparison of six <em>Peronospora effusa</em> isolates. For each isolate, we provide the genome assemblies, gene and repeat annotation, effector clustering, and gene variation. Additionally, we provide the repeat library that was used to annotate the transposable elements for each isolate and the pangenome graph.</p> <p>DOI: https://doi.org/10.1101/2024.05.30.596583 </p>
Trinity assembly of Downy mildew of Grapevine lesion samples DMG-G
<p>De Novo trinity assembly RNAseq NGS-Data</p>
Trinity assembly of Downy mildew of Grapevine lesion samples DMG-A
<p>Tirnity Assembly of NGS reads </p>
Trinity assembly of Downy mildew of Lettuce lesion samples
<p>De novo trinity assembly of RNAseq NGS data</p>
Data from: Soft selective sweeps in fungicide resistance evolution: recurrent mutations without fitness costs in grapevine downy mildew
Adaptation produces hard or soft selective sweeps depending on the supply of adaptive genetic polymorphism. The evolution of pesticide resistance in parasites is a striking example of rapid adaptation that can shed light on selection processes. Plasmopara viticola, which causes grapevine downy mildew, forms large populations, in which resistance has rapidly evolved due to excessive fungicide use. We investigated the pathways by which fungicide resistance has evolved in this plant pathogen, to determine whether hard or soft selective sweeps were involved. An analysis of nucleotide polymorphism in 108 field isolates from the Bordeaux region revealed recurrent mutations of cytb and CesA3 conferring resistance to quinone outside inhibiting (QoI) and carboxylic acid amide (CAA) fungicides, respectively. Higher levels of genetic differentiation were observed for nucleotide positions involved in resistance than for neutral microsatellites, consistent with local adaptation of the pathogen to fungicide treatments. No hitchhiking was found between selected sites and neighbouring polymorphisms in cytb and CesA3, confirming multiple origins of resistance alleles. We assessed resistance costs, by evaluating the fitness of the 108 isolates through measurements of multiple quantitative pathogenicity traits under controlled conditions. No significant differences were found between sensitive and resistant isolates, suggesting that fitness costs may be absent or negligible. Our results indicate that the rapid evolution of fungicide resistance in P. viticola has involved a soft sweep.
Data from: Adaptation of a plant pathogen to partial host resistance: selection for greater aggressiveness in grapevine downy mildew
An understanding of the evolution of pathogen quantitative traits in response to host selective pressures is essential for the development of durable management strategies for resistant crops. However, we still lack experimental data on the effects of partial host resistance on multiple phenotypic traits (aggressiveness) and evolutionary strategies in pathogens. We performed a cross-inoculation experiment with four grapevine hosts and 103 isolates of grapevine downy mildew (Plasmopara viticola) sampled from susceptible and partially resistant grapevine varieties. We analysed the neutral and adaptive genetic differentiation of five quantitative traits relating to pathogen transmission. Isolates from resistant hosts were more aggressive than isolates from susceptible hosts, as they had a shorter latency period and higher levels of spore production. This pattern of adaptation contrasted with the lack of neutral genetic differentiation, providing evidence for directional selection. No specificity for a particular host variety was detected. Adapted isolates had traits that were advantageous on all resistant varieties. There was no fitness cost associated with this genetic adaptation, but several trade-offs between pathogen traits were observed. These results should improve the accuracy of prediction of fitness trajectories for this biotrophic pathogen, an essential element for the modelling of durable deployment strategies for resistant varieties.
Data from: Daytime solar heating controls downy mildew Peronospora belbahrii in sweet basil
The biotrophic oomycete Peronospora belbahrii causes a devastating downy mildew disease in sweet basil. Due to the lack of resistant cultivars current control measures rely heavily on fungicides. However, resistance to fungicides and strict regulation on their deployment greatly restrict their use. Here we report on a 'green' method to control this disease. Growth chamber studies showed that P. belbahrii could hardly withstand exposure to high temperatures; exposure of spores, infected leaves, or infected plants to 35-45°C for 6-9 hours suppressed its survival. Therefore, daytime solar heating was employed in the field to control the downy mildew disease it causes in basil. Covering growth houses of sweet basil already infected with downy mildew with transparent infra-red-impermeable, transparent polyethylene sheets raised the daily maximal temperature during sunny hours by 11-22°C reaching 40-58°C (greenhouse effect). Such coverage, applied for a few hours during 1-3 consecutive days, had a detrimental effect on the survival of P. belbahrii: killing the pathogen and/or suppressing disease progress while enhancing growth of the host basil plants.
The genome of Vitis vinifera cv. Mgaloblishvili reveals resistance and susceptibility factors to downy mildew in the Rpv29 and Rpv31 loci
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Data from 36 fungicide trials on grape downy mildew across Europe 2012-2019
<p>The data set comprises records of disease incidence and yield from untreated and treated plots.</p> <p>The data is provided as both a tab-separated text file and a binary R data file. The R files provides code to read and plot the data. The plot produced is also provided as a PNG file.</p> <p>The field trials were conducted by BASF, Germany.</p> <p><em>Details</em><br> The PlantPart column refers to leaves (LEAF), inflorescences (RACEME) and grape clusters (FRUIT). The disease (GDM) was assessed by two methods (Method column), designated P%FREQ and P%INF:<br> • P%INF: Intensity of attack was obtained as a visual estimation of the percentage of each plant part (leaf, raceme or grape cluster) affected by disease.<br> • P%FREQ: Frequency of attack was obtained as the number of infected plant parts (leaf, raceme or grape cluster). The frequency was expressed as a percentage of the number sampled.</p> <p> </p>
Trinity assembly of Downy mildew of Grapevine lesion samples DMG-F
<p>De novo Trinity assembly of RNAseq NGS data</p>
Trinity assembly of Downy mildew of Grapevine lesion samples DMG-D
<p>de novo trinity assembly of RNA seq NGS Data</p>
Trinity assembly of grapevine downy mildew lesion- DMG-B
<p>DE novo Trinity assembly of NGS reads from RNAseq, transcriptome</p>
Molecular markers used to test brassica oleracea for resistance to downy mildew
<p>Molecular markers used to test brassica oleracea for resistance to downy mildew</p>
Data from: Genetic signature of a range expansion and leap-frog event after the recent invasion of Europe by the grapevine downy mildew pathogen Plasmopara viticola
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Data from: Adaptation of a plant pathogen to partial host resistance: selection for greater aggressiveness in grapevine downy mildew
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Data from: Daytime solar heating controls downy mildew Peronospora belbahrii in sweet basil
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Data from: Soft selective sweeps in fungicide resistance evolution: recurrent mutations without fitness costs in grapevine downy mildew
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Super pangenome of Vitis empowers identification of downy mildew resistance genes for grapevine improvement
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Fig. 2. Key HMBC and COSY correlations for compound 1a in Ingadosides A-C, acacic acid-type saponins from Inga sapindoides with potent inhibitory activity against downy mildew
Fig. 2. Key HMBC and COSY correlations for compound 1a.
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OpenNeuro
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