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196 results for “ERK”
ERK KTR translocation after pulsatile optoFGFR stimulation. Dataset for Nalecz-Jawecki et al.
<p>Quantified microscopic data: Single cell measurements of ERK activity reporter (ERK KTR) translocation after stimulation of optoFGFR with 100 ms blue (470 nm) light pulses in random points of time. Dataset used in Nałęcz-Jawecki et al. to quantify information transmission rate throught the MAPK/ERK pathway. Preprint available at BioArxiv: https://doi.org/10.1101/2022.03.17.484713.</p> <p>UPDATE: v1.1 contains one experiment file mising in v1.0</p>
ERK activity in migrating MDA-MB-231 cells (clover-ERK-KTR + sir-DNA)
<p>MDA-MB-231 or U2OS cells stably expressing clover-ERK-KTR were seeded on fibronectin-coated (1 µg /ml) Ibidi 8-well slides (Ibidi) 1 day before imaging. Four hours before imaging, the medium was supplemented with 250 nM sir-DNA (Cytoskeleton) and 25 mM HEPES (Sigma). Cells were then imaged live (37 °C, 5% CO<sub>2</sub>) using a Nikon Eclipse Ti2-E microscope (Nikon) equipped with an sCMOS Orca Flash4.0 camera (Hamamatsu) and controlled by the NIS-Elements software (Nikon, v 5.11.01). MDA-MB-231 cells were imaged using a 20× Nikon CFI Plan Apo Lambda objective (NA 0.75), either 1 frame per minute for 2 hours or 1 frame every 5 minutes for 17 hours. In these experiments, a camera binning of 2 × 2 was used. </p> <p>This dataset consists of 20 videos.</p>
ERK signaling of invasive cells in mammary organoid
<p>Live-cell imaging of HMT3522 T4-2 invasive mammary epithelial cell line, with fluorescent ERKTR cell signaling reporter and nuclear reporter, to extract trajectory pairs of ERKTR to nuclear cross-correlation as a measure of ERK signaling activity. Datasets X and Y capture ERK signaling state at t and t+1 respectively, while test_obs contains test trajectories and inds_test_starts contains the indices of starting times of test trajectories.</p>
Cell migration with ERK signalling
<p>Movie following cells expressing ERK and a nuclei staining, tracked with TrackMate and later analyzed with MATLAB.</p> <p>See https://imagej.net/plugins/trackmate/trackmate-stardist for more details.</p> <p> </p>
The kinase ERK plays a conserved dominant role in the heterogeneity of epithelial-mesenchymal transition in pancreatic cancer cells
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Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells
<p>Signal transduction networks generate characteristic dynamic activities to process extracellular signals and guide cell fate decisions such as to divide or differentiate. The differentiation of pluripotent cells is controlled by FGF/ERK signaling. However, only a few studies have addressed the dynamic activity of the FGF/ERK signaling network in pluripotent cells at high time resolution. Here, we use live cell sensors in wild-type and Fgf4-mutant mouse embryonic stem cells to measure dynamic ERK activity in single cells, for defined ligand concentrations and differentiation states. These sensors reveal pulses of ERK activity. Pulsing patterns are heterogeneous between individual cells. Consecutive pulse sequences occur more frequently than expected from simple stochastic models. Sequences become more prevalent with higher ligand concentration, but are rarer in more differentiated cells. Our results suggest that FGF/ERK signaling operates in the vicinity of a transition point between oscillatory and non-oscillatory dynamics in embryonic stem cells. The resulting heterogeneous dynamic signaling activities add a new dimension to cellular heterogeneity that may be linked to divergent fate decisions in stem cell cultures.</p>
Single cell imaging of ERK and Akt activation dynamics and heterogeneity induced by G protein-coupled receptors - Scripts & Source data
<p>Source data and scripts to reproduce the figures that are part of the publication "Single cell imaging of ERK and Akt activation dynamics and heterogeneity induced by G protein-coupled receptors".</p> <p>Journal of Cell Science (2022) 135, jcs259685, DOI: 10.1242/jcs.259685</p> <p> </p> <p>An earlier version of this work is published as a preprint: "Heterogeneity and dynamics of ERK and Akt activation by G protein-coupled receptors depend on the activated heterotrimeric G proteins", DOI: <a href="https://doi.org/10.1101/2021.07.27.453948">10.1101/2021.07.27.453948</a></p>
Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells
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Oncogenic BRAF V600E induces glial proliferation through ERK and neuronal death through JNK
<p>Raw Data Files for the manuscript entitled "Oncogenic <em>BRAF</em> V600E induces glial proliferation through ERK and neuronal death through JNK". </p>
Analysis of localized cAMP perturbations within a tissue reveal the effects of a local, dynamic gap junction state on ERK signaling
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Oncogenic BRAF V600E induces glial proliferation through ERK and neuronal death through JNK
<p>Background: Activating V600E in BRAF is a common driver mutation in cancers of multiple tissue origins, including melanoma and glioma. BRAF V600E has also been implicated in neurodegeneration. The present study aims to characterize BRAF V600E on cell death and survival in three major cell types of the CNS: neurons, astrocytes, and microglia. Methods : Multiple primary cultures and cell lines of glial cells and neurons were employed. BRAF V600E as well as BRAF WT expression was mediated by lentivirus or retrovirus. Blockage of downstream effectors were achieved by siRNA. Gene expression data from patients with Parkinson’s disease was analyzed. Results : In astrocytes and microglia, BRAF V600E induces cell proliferation, and the proliferative effect in microglia is mediated by activated ERK but not JNK. Conditioned medium from BRAF V600E -expressing microglia induced neuronal cell death. In neuronal cells, BRAF V600E directly induces cell death, through JNK but not ERK. We further show that BRAF-related genes are enriched in pathways in patients with Parkinson’s disease. Conclusions : Our study identifies distinct consequences mediated by distinct downstream effectors in dividing glial cells and in neurons following the same BRAF mutational activation and a causal link between BRAF-activated microglia and neuronal cell death that does not require physical proximity. It provides insight into a possibly important role of BRAF in neurodegeneration as a result of either dysregulated BRAF in neurons or its impact on glial cells.</p>
Ascorbic acid ameliorates corneal endothelial dysfunction and enhances cell proliferation via the noncanonical GLUT1-ERK axis
<p>The datasets supporting the conclusions of the article submitting.</p>
Data from: MFAP5 contributes to inflammation, synovial hyperplasia and joint damage in rheumatoid arthritis via integrin-ERK signaling
<p>Synovial hyperplasia is the primary pathological changes of RA and FLS is the principal etiological cellular component that drives and constitutes the pathological lesions. To elucidate the molecular mechanism of how FLS may contribute to RA, we performed transcriptome profiling to determine the DEGs between normal and RA FLS. We compared the primary FLS isolated from healthy control (HFLS) to that isolated from RA patients (RA FLS), and also to an immortalized RA FLS cell line MH7A immortalized by stably transfecting primary RA FLS cells with SV40 T antigen gene. </p>
Data from: Cavin-3 dictates the balance between ERK and Akt signaling
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Data from: MFAP5 contributes to inflammation, synovial hyperplasia and joint damage in rheumatoid arthritis via integrin-ERK signaling
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Dynamic Lineage Priming by ERK is Driven by Transcription Factor-Independent Enhancer Regulation [mRNA_TC]
GEO Series GSE132497. Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.
The Lysine Demethylase Kdm4C is an Oncogenic Driver and Regulates ERK Activity in KRAS Mutant Pancreatic Ductal Adenocarcinoma [RNA-Seq]
GEO Series GSE300083. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Small molecule inhibition of ERK dimerization prevents tumorigenesis by Ras-ERK pathway oncogenes
GEO Series GSE68230. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
ERK signaling mediates resistance to immunomodulatory drugs in the bone marrow microenvironment (RNA-Seq)
GEO Series GSE171340. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Erk-dependent transcriptome
GEO Series GSE110662. Mus musculus. 8 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.