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FIG. 2. — Oocyste d in Description d'une nouvelle espèce d'Eimeria (Coccidia, Eimeridea) chez le lapin de garenne Oryctolagus cuniculus en France

FIG. 2. — Oocyste d' Eimeria roobroucki n. sp. (holotype), n° d'enregistrement: P301LV. Échelle: 10 µm.

opencc-zeroFeb 2002View details →
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Fig. 2 in Reclassification of Eimeria pogonae Walden (2009) as Choleoeimeria pogonae comb. nov. (Apicomplexa: Eimeriidae)

Fig. 2 Photographs of ooccyst of Choleoeimaria pogonae. a Sporulated oocyst containing four sporocysts each with two sporozoites. Note compact sporocyst residuum (csr). b Sporulated oocyst isolated from the gallbladder with eight sporozoites released from sporocysts. Note polar granule (pg), posterior refractile body of the sporozoite (prb) and sporozoite nucleus (n). c Line drawings of sporulated oocysts. All in the same scale bar=10 μm

opencc-by-4.0Oct 2015View details →
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Fig. 1–3 in A new species of Eimeria Schneider, 1875 from the Serra dos Órgãos National Park, Rio de Janeiro, Brazil, with notes on its endogenous development in the montane grass mouse, Akodon montensis Thomas, 1913 (Rodentia: Sigmodontinae)

Fig. 1–3 Nomarski interference-contrast photomicrographs of Eimeria akodonensis n. sp. from the montane grass mouse, Akodon montensis. Presence of oocyst residuum (Or) and highly refractile polar granule (Pg); Sporocyst residuum (Sr); wall (Ow); Stieda body (Sb); sporozoite (Sp). Scale bar = 10 μm

opencc-by-4.0Dec 2017View details →
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Fig. 1 in Reclassification of Eimeria pogonae Walden (2009) as Choleoeimeria pogonae comb. nov. (Apicomplexa: Eimeriidae)

Fig. 1 Macroscopically visible pathological changes of the gallbladder in infected lizards. Local white wall thickening (a). Gallstones and debris inside the gallbladder (b)

opencc-by-4.0Oct 2015View details →
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Fig. 5–12 in A new species of Eimeria Schneider, 1875 from the Serra dos Órgãos National Park, Rio de Janeiro, Brazil, with notes on its endogenous development in the montane grass mouse, Akodon montensis Thomas, 1913 (Rodentia: Sigmodontinae)

Fig. 5–12 Light micrographs of the endogenous stages of Eimeria akodonensis n. sp. observed in the lamina propria of the small intestine of Akodon montensis, showing the microgamonts (Mi) and the parasitophorous vacuole in the host cell (Vp); microgametes (Mi); macrogamonts (Ma); nucleus (N) and the zygote (Zy) with wallforming bodies arranged around its periphery (Ow); oocyst (Oo). Scale bar = 10 μm

opencc-by-4.0Dec 2017View details →
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Fig. 4 in A new species of Eimeria Schneider, 1875 from the Serra dos Órgãos National Park, Rio de Janeiro, Brazil, with notes on its endogenous development in the montane grass mouse, Akodon montensis Thomas, 1913 (Rodentia: Sigmodontinae)

Fig. 4 Composite line drawing of the sporulated oocyst of Eimeria akodonensis n. sp. Scale bar = 10 μm

opencc-by-4.0Dec 2017View details →
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Fig. 3 in Reclassification of Eimeria pogonae Walden (2009) as Choleoeimeria pogonae comb. nov. (Apicomplexa: Eimeriidae)

Fig. 3 Various endogenous stages of coccidia. Note infected cells are displaced into the lumen of bile duct

opencc-by-4.0Oct 2015View details →
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Figure 6 in Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella

Figure 6. Localization of EtCHP18905 in infected DF-1 cells by indirect immunofluorescence. Parasites incubated with anti-rEtCHP18905, stained with FITC (green)-conjugated secondary antibodies, and counterstained with DAPI (blue). Infected DF-1 cells were collected at indicated time points post-infection. (A) Sporozoites (Spz) in PBS, pRB, posterior refractile body; (B) Spz in complete medium. Infected DF-1 cells were collected at the indicated time points post-infection (pi); (C) 2 hours pi (hpi); (D) immature schizonts (iSC) 48 hpi; (E) mature schizonts (mSC) 72 hpi; (F) merozoites (Mrz) in PBS.

opencc-by-4.0May 2021View details →
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Figure 3 in Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella

Figure 3. Expression and purification of rEtCHP18905. (A) SDS-PAGE analysis of the 5rEtCHP18905. Lanes 1, protein marker; 2, negative control (not induced with IPTG); 3, the rEtCHP18905 protein with the GST-tag protein of the vector (induced with IPTG for 6 h). (B) Western blot analysis of purified rEtCHP18905 protein. Lane 2, protein recognized by an anti GST-Tag monoclonal antibody. (C) Western blot analysis of purified rEtCHP18905 protein. Lane 2, protein recognized by rabbit sera against sporozoite, lane 4 incubated with naïve rabbit serum.

opencc-by-4.0May 2021View details →
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Figure 2 in Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella

Figure 2. Bioinformatic analysis of EtCHP18905. The stop codon is indicated with an asterisk. N-myristoylation sites are double underlined. The transmembrane domain is shaded yellow with black lettering. N-glycosylation sites are surrounded by a black box. cAMP- and cGMP-dependent protein kinase phosphorylation sites are shaded black with white lettering. Tyrosine kinase phosphorylation site is shaded grey with black lettering. Casein kinase II phosphorylation sites are indicated with red lettering. Protein kinase C phosphorylation sites are underlined by a wavy line.

opencc-by-4.0May 2021View details →
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Figure 1 in Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella

Figure 1. Multiple alignment analysis of EtCHP18905 with other Eimeria proteins. DNAMAN was used to analyze the deduced protein sequences. The identical amino acids are listed at the bottom. NCBI reference sequence accession numbers: Eimeria tenella, XP_013231819, Eimeria necatrix, XP_013438465, Eimeria mitis, XP_013355934, Eimeria maxima, XP_013336337, Eimeria acervulina, XP_013251133; GenBank accession numbers: Eimeria praecox, CDI76926, Eimeria brunetti, CDJ52365.

opencc-by-4.0May 2021View details →
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Figure 7 in Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella

Figure 7. Inhibition of sporozoite invasion in vitro by antirEtCHP18905. Anti-rEtCHP18905, rabbit anti-rEtCHP18905 IgG; NA, naïve rabbit sera IgG; GST control, rabbit anti-GST IgG. The symbol "*" represents p <0.05, "**" represents p <0.01, and "***" represents p <0.001 for comparison of treatment with antirEtCHP18905 and naïve rabbit sera IgG and anti-GST IgG at the same concentration. The error bars indicate the standard deviation. All assays were performed in triplicate.

opencc-by-4.0May 2021View details →
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Figure 4 in Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella

Figure 4. Transcription levels of EtCHP18905 in different developmental stages of E. tenella. UO, unsporulated oocysts; SO, sporulated oocysts; Spz, sporozoites; Mrz, merozoites. Bars with different letters indicate significantly different expression levels (p <0.05) and the error bars indicate standard deviations.

opencc-by-4.0May 2021View details →
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Figure 5 in Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella

Figure 5. Expression levels of EtCHP18905 in different developmental stages of E. tenella. (A) Western blot of the internal reference tubulin and EtCHP18905 protein. (B) Relative expression levels of the EtCHP18905 protein. Bars with different letters indicate significantly different expression levels (p <0.05) and the error bars indicate standard deviations.

opencc-by-4.0May 2021View details →
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Figure 6 in Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1

Figure 6. KEGG pathway classification of differentially expressed proteins in DF-1 cells transiently transfected with EtAMA1.

opencc-by-4.0Dec 2020View details →
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Figure 5 in Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1

Figure 5. Gene ontology analysis of 163 proteins differentially expressed in DF-1 cells transiently transfected with EtAMA1. Proteins were annotated based on biological process, cellular component, and molecular function.

opencc-by-4.0Dec 2020View details →
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Figure 3 in Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1

Figure 3. Inhibition of sporozoite invasion in vitro by antibodies against rEtAMA1, rEtESP, and rEtRON2. (a) Invasion-inhibition activities of single antibodies. Anti-rEtAMA1, rEtESP, and rEtRON2 rabbit anti-serum against recombinant EtAMA1, EtESP and EtRON2 protein, respectively; IgG, normal rabbit serum. (b) Invasion-inhibition activities of antibody combinations. Combinations of anti-rEtAMA1 and antirEtESP or anti-rEtRON2 were added at a ratio of 1:1 to generate a gradient concentration of IgG. All assays were performed in triplicate. *p <0.05, **p <0.01 and ***p <0.001, as determined by the Student's t-test versus the non-immunized IgG groups at the same concentration.

opencc-by-4.0Dec 2020View details →
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Figure 2 in Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1

Figure 2. Colocalization of EtAMA1, EtESP, and EtRON2 in sporozoites by indirect immunofluorescence. Parasites were immunostained with anti-rEtAMA1, and anti-rEtESP or anti-rEtRON2 antibodies, visualized with FITC (green) and counter-stained with DAPI (blue). Scale bar, 10 µm.

opencc-by-4.0Dec 2020View details →
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Figure 1. EtAMA1 in Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1

Figure 1. EtAMA1 is secreted by micronemes. (a) EtAMA1 secretion is FCS- and temperature-dependent. Fresh sporozoites were incubated in PBS or complete medium (CM) at 4 °C or 41 °C for 2 h. Supernatants containing excretory-secretory antigens (ESAs) were harvested and analyzed by western blotting to detect EtAMA1 and EtMIC2. (b) EtAMA1 secretion is inhibited by staurosporine. Sporozoites were incubated in CM with various concentrations of staurosporine or DMSO at 41 °C for 2 h. Supernatants containing ESAs was harvested and analyzed by western blotting to detect EtAMA1 and EtMIC2.

opencc-by-4.0Dec 2020View details →
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Figure 4 in Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1

Figure 4. In vitro sporozoite invasion of DF-1 cells transiently transfected with EtAMA1. (a) Verification of pcDNA3.1-(+)-EtAMA1 expression in DF-1 cells by IFA. (b) The proliferation of DF-1 cells transfected with pcDNA3.1-(+)-EtAMA1 or pcDNA3.1-(+). (c) Sporozoite invasion rate in DF-1 cells transfected with pcDNA3.1-(+)-EtAMA1 or pcDNA3.1-(+). *p <0.05 and **p <0.01, as determined by the Student's t-test versus the untreated group.

opencc-by-4.0Dec 2020View details →

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