Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
22
datasets available to search
ShareScore release 0.9.0
Dataset results
22 results for “Endolysosome”
Raw image files in Fig. S1E associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"
<p>This entry contains raw ND2 files shown in Fig. S1E associated with: https://doi.org/10.1101/2024.09.24.614704</p>
Raw image files of uncropped western blots associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"
<p>This entry contains uncropped blots for Fig. 1C, S1C, S1D, 2I, and S3B for: https://doi.org/10.1101/2024.09.24.614704</p>
Raw mass spectrometry data for publication "Vertebrate cellular endolysosome modulating pore-forming protein is negatively regulated by its homologue under environmental oxidative conditions"
<p><strong>Abstract</strong>: Endolysosomes are key players in cell physiology, including material exchange, immunity and environmental adaptation etc. Bacterial pore-forming toxin aerolysin-like proteins (ALPs) are widely distributed in animals and plants. βγ-CAT is a complex of an ALP (BmALP1) and a trefoil factor (BmTFF3) in the frog <em>Bombina maxima</em>. It is the first example that a secreted endogenous pore-forming protein modulates the biochemical properties of endolysosomes via pore formation in these vesicles. Here, we report the identification of BmALP3, a homologue of BmALP1 that lacks membrane pore formation capacity. Both BmALP3 and BmALP1 contain a conserved cysteine in their C-terminal regions. BmALP3 was readily oxidized to disulfide bond linked homodimer, and the homodimer could then oxidize BmALP1 via disulfide bond exchange, resulting in the dissociation of βγ-CAT subunits and elimination of its biological activity. Consistent with its behavior <em>in vitro</em>, BmALP3 senses environmental oxygen tension <em>in vivo</em>, leading to modulation of βγ-CAT activity. Interestingly, this C-terminal cysteine site is well conserved in numerous vertebrate ALPs. These findings, for the first time, uncovered the existence of a regulatory ALP (BmALP3) and its modulating action on a cell executive ALP (BmALP1) in a redox-dependent manner, which is completely different from that of bacterial toxin aerolysins.</p>
Key Resource Table associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"
<p>This entry contains a Key Resource table associated with: https://doi.org/10.1101/2024.09.24.614704</p>
CRISPR/Cas9 screen for essential endolysosomal genes under free choline limitation
<p>Lysosomes degrade macromolecules and recycle their nutrient content to support cell function and survival. Yet, the machineries involved in lysosomal recycling of many nutrients remain to be discovered, with a notable example being choline, an essential metabolite liberated via phospholipid degradation. Here, we engineered metabolic dependency on lysosome-derived choline in pancreatic cancer cells to perform an endolysosome-focused CRISPR-Cas9 screen for genes mediating lysosomal choline recycling. We identified the orphan lysosomal transmembrane protein SPNS1 as critical for cell survival under choline limitation. SPNS1 loss leads to intralysosomal accumulation of lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE). Mechanistically, we reveal that SPNS1 is a proton gradient-dependent transporter of LPC species from the lysosome for their re-esterification into phosphatidylcholine in the cytosol. Finally, we establish that LPC efflux by SPNS1 is required for cell survival under choline limitation. Collectively, our work defines a lysosomal phospholipid salvage pathway that is essential under nutrient limitation, and more broadly, provides a robust platform to deorphan lysosomal gene function.</p>
Secretome dataset for: The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence
Open the record for dataset details and reuse information.
Proteomic dataset for: The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence
Open the record for dataset details and reuse information.
CRISPR/Cas9 screen for essential endolysosomal genes under free choline limitation
Open the record for dataset details and reuse information.
Transcriptome dataset for: The ESCRT protein CHMP5 restricts bone formation by controlling endolysosome-mitochondrion-mediated cell senescence
Open the record for dataset details and reuse information.
Source data for Fig. S1F associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"
<p>This entry contains source data and statistical test for plots in Fig. S1F associated with: https://doi.org/10.1101/2024.09.24.614704</p>
Supplementary datasets associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"
<p>This entry contains supplementary dataset files associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons".</p>
Endolysosomal dysfunction in radial glia progenitor cells leads to defective cerebral angiogenesis and compromised blood-brain barrier integrity
GEO Series GSE270309. Danio rerio. 6 samples. Type: Expression profiling by high throughput sequencing.
Impact of SLC15A4, TASL and TASL2(Gm6377) deficiency on endolysosomal TLR7 and TLR9 responses
GEO Series GSE267786. Mus musculus. 95 samples. Type: Expression profiling by high throughput sequencing.
STING phosphoserine 358 engages IRF3 to activate NF-kB at late endolysosomal compartments
GEO Series GSE305060. Gallus gallus; Chelonoidis carbonarius; Cyprinus carpio; Mus musculus; Homo sapiens. 30 samples. Type: Expression profiling by high throughput sequencing.
Angiotensin Converting Enzyme (ACE) expression in microglia reduces amyloid β deposition and neurodegeneration by increasing SYK signaling and endolysosomal trafficking
GEO Series GSE288280. Mus musculus. 67 samples. Type: Other.
QKI deficiency maintains stemness of glioma stem cells in suboptimal environment by downregulating endolysosomal degradation
GEO Series GSE84134. Mus musculus. 34 samples. Type: Expression profiling by high throughput sequencing.
MITF drives endolysosomal biogenesis and potentiates Wnt signaling in melanoma cells
GEO Series GSE64497. Homo sapiens. 51 samples. Type: Expression profiling by array.
Neuropathy-associated Tecpr2 mutation knock-in mice reveal endolysosomal loss of function phenotypes in neurons and microglia
GEO Series GSE284475. Mus musculus. 16 samples. Type: Expression profiling by high throughput sequencing.
Compartmentalisation proteomics revealed endolysosomal protein network changes in goat model of atrial fibrillation
GEO Series GSE228289. Capra hircus. 8 samples. Type: Expression profiling by high throughput sequencing.
Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice.
GEO Series GSE286044. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.