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2,271 results for “Endothelial cells”
Data for a publication "Argon plasma-modified bacterial nanocellulose: Cell-specific differences in the interaction with fibroblasts and endothelial cells"
<p>A dataset containing data for the published article "Argon plasma-modified bacterial nanocellulose: Cell-specific differences in the interaction with fibroblasts and endothelial cells".</p> <p> </p> <p>For more details, please read the <strong>README - Description of data and analysis informations.txt</strong> file.</p> <p><strong>Dataset versions:</strong></p> <p><strong>V1:</strong> The first dataset containing a majority of the data.</p> <p><strong>V2:</strong> Dataset contains all the data mentioned in the article in the appropriate file formats for long-term preservation and accessibility.</p>
Data from: Selectivity of Guanine Nucleotide Exchange Factor-mediated Cdc42 activation in primary human endothelial cells
<p>Data that was reported in "Selectivity of Guanine Nucleotide Exchange Factor-mediated Cdc42 activation in primary human endothelial cells" by </p> <p>Nathalie R. Reinhard<sup>1</sup>, Sanne van der Niet<sup>1</sup>, Anna Chertkova<sup>1</sup>, Marten Postma<sup>1</sup>, Theodorus W.J. Gadella Jr.<sup>1</sup>, Peter L. Hordijk<sup>1,2</sup>, and Joachim Goedhart<sup>1*</sup><br> </p> <p><strong>Affiliations:</strong></p> <p><sup>1 </sup>University of Amsterdam, Molecular Cytology, Swammerdam Institute for Life Sciences, van Leeuwenhoek Centre for Advanced Microscopy, Amsterdam, the Netherlands</p> <p><sup>2 </sup>Department of Physiology, Free University Medical Center, Amsterdam, The Netherlands</p> <p> </p> <p>*Correspondence to: j.goedhart@uva.nl</p>
Reference model and embedding for human kidney endothelial cell mapping
<p>Reference model and embedding for human kidney endothelial cell mapping</p><p>The reference model serves as a basis for the mapping of new data to the HLCA using scArches (Lotfollahi et al., https://doi.org/10.1038/s41587-021-01001-7). </p>
Data from: Hypoxia blunts angiogenic signaling and upregulates the antioxidant system in elephant seal endothelial cells
<p><strong><em>Background</em></strong></p> <p>Elephant seals exhibit extreme hypoxemic tolerance derived from repetitive hypoxia/reoxygenation episodes they experience during diving bouts. Real-time assessment of the molecular changes underlying protection against hypoxic injury in seals remains restricted by their at-sea inaccessibility. Hence, we developed a proliferative arterial endothelial cell culture model from elephant seals and used RNA-seq, functional assays, and confocal microscopy to assess the molecular response to prolonged hypoxia.</p> <p><strong><em>Results</em></strong></p> <p>Seal and human endothelial cells exposed to 1% O<sub>2</sub> for up to 6 h respond differently to acute and prolonged hypoxia. Seal cells decouple stabilization of the hypoxia-sensitive transcriptional regulator HIF-1α from angiogenic signaling. Rapid upregulation of genes involved in glutathione (GSH) metabolism supports the maintenance of GSH pools, and intracellular succinate increases in seal but not human cells. High maximal and spare respiratory capacity in seal cells after hypoxia exposure occurs in concert with increasing mitochondrial branch length and independent from major changes in extracellular acidification rate, suggesting that seal cells recover oxidative metabolism without significant glycolytic dependency after hypoxia exposure.</p> <p><strong><em>Conclusions</em></strong></p> <p>We found that the glutathione antioxidant system is upregulated in seal cells during hypoxia, while this system remains static in comparable human cells. Furthermore, we found that in contrast to human cells, hypoxia exposure rapidly activates HIF-1 in seal cells, but this response is decoupled from the canonical HIF-angiogenesis pathway. These results highlight the unique mechanisms that confer extraordinary tolerance to limited oxygen availability in a champion diving mammal.</p>
Long read proteogenomics to characterize protein isoform diversity in human umbilical vein endothelial cells (HUVECs)
<p>Endothelial cells (ECs) comprise the lumenal lining of all blood vessels and are critical for the functioning of the cardiovascular system and their phenotypes can be modulated by protein isoforms. To characterize the isoform landscape within EC, we applied a long read proteogenomics approach to analyze human umbilical vein endothelial cells (HUVECs). Transcripts delineated from PacBio sequencing serve as the basis for a sample-specific protein database used for downstream MS analysis to infer protein isoform expression. We detected 53,836 transcript isoforms from 10,426 genes, with 22,195 of those transcripts being novel. Furthermore, the predominant isoform in HUVECs does not correspond with the accepted “reference isoform” 25% of the time, with vascular pathway-related genes among this group. We found 2,597 protein isoforms supported through unique peptides, with an additional 2,280 isoforms nominated upon incorporation of long-read transcript evidence. We characterized a novel alternative acceptor for endothelial-related gene <em>CDH5</em>, suggesting potential changes in its associated signaling pathways. Finally, we identified novel protein isoforms arising from a diversity of splicing mechanisms supported by uniquely mapped novel peptides. Our results represent a high resolution atlas of known and novel isoforms of potential relevance to endothelial phenotypes and function.</p>
TIRF imaging data of neutrophils migrating underneath endothelial cells
<p>This data is used in the publication "Endothelial Focal Adhesions Are Functional Obstacles for Leukocytes During Basolateral Crawling": https://www.frontiersin.org/articles/10.3389/fimmu.2021.667213/full</p> <p> </p> <p><strong>TIRF Microscopy</strong></p> <p>Lentiviral transduction was used to generate an endothelial cell line expressing mNeonGreen-Paxillin (derived from addgene plasmid # 129604). Cells were imaged with a Nikon Ti-E microscope equipped with a motorized TIRF Illuminator unit, a 60x TIRF objective (60x Plan Apo, Oil DIC N2, NA =1.49, WD = 120 um) and Perfect Focus System. Images were acquired with an Andor iXon 897 EMCCD camera and the Nikon NIS elements software. mNeonGreen was imaged using the 488 nm laser line and calcein red-orange was imaged using the 561 nm laser line. A quad split dichroic mirror (405 nm, 488 nm, 561 nm, 640 nm) was used in combination with dual band pass emission filter (515 to 545 nm, 600 to 650 nm). To achieve a larger field of view a 3 x 3 tile scans was acquired with 15% overlap stitching on the GFP channel. Time lapse images were taken every 10 s.</p>
Dataset related to: Empagliflozin protects glomerular endothelial cell architecture in experimental diabetes through the VEGF-A/caveolin-1/PV-1 signaling pathway
<p>The files contain all the dataset included in the manuscript divided by figures.</p> <p>Abstract<br> In addition to having blood glucose-lowering effects, inhibitors of sodium glucose cotransporter 2 (SGLT2) afford renoprotection in diabetes. We sought to investigate which components of the glomerular filtration barrier could be involved in the antiproteinuric and renoprotective effects of SGLT2 inhibition in diabetes. BTBR (black and tan, brachyuric) <em>ob/ob</em> mice that develop a type 2 diabetic nephropathy received a standard diet with or without empagliflozin for 10 weeks, starting at 8 weeks of age, when animals had developed albuminuria. Empagliflozin caused marked decreases in blood glucose levels and albuminuria but did not correct glomerular hyperfiltration. The protective effect of empagliflozin against albuminuria was not due to a reduction in podocyte damage as empagliflozin did not affect the larger podocyte filtration slit pore size nor the defective expression of nephrin and nestin. Empagliflozin<br> did not reduce the thickening of the glomerular basement membrane. In BTBR <em>ob/ob</em> mice, the most profound abnormality seen using electron microscopy was in the endothelial aspect of the glomerular capillary, with significant loss of endothelial fenestrations. Remarkably, empagliflozin ameliorated the subverted microvascular endothelial ultrastructure. Caveolae and bridging diaphragms between adjacent endothelial fenestrae were seen in diabetic mice and associated with increased expression of caveolin-1 and the appearance of PV-1. These endothelial abnormalities were limited by the SGLT2 inhibitor. Although no expression of SGLT2 was found in glomerular endothelial cells, SGLT2 was expressed in the podocytes of diabetic mice. VEGF-A, which is a known stimulus for endothelial caveolin-1 and PV-1, was increased in podocytes of BTBR <em>ob/ob</em> mice and normalized by SGLT2 inhibitor treatment.<br> Thus, empagliflozin’s protective effect on the glomerular endothelium of diabetic mice could be due to a limitation of the paracrine signaling of podocyte-derived VEGF-A that resulted in a reduction of the abnormal endothelial caveolin-1 and PV-1, with the consequent preservation of glomerular endothelial function and permeability.</p>
Impact of Donor Diabetes on DMEK Success and Endothelial Cell Loss
ClinicalTrials.gov study NCT05134480. IPD Sharing: YES. Countries: 1. Publications: 3.
Data from: Hypoxia blunts angiogenic signaling and upregulates the antioxidant system in elephant seal endothelial cells
Open the record for dataset details and reuse information.
Characterization of photorecruitable Guanine Exchange Factors for the study of Rho GTPases in endothelial cells
<p>Time lapses of respective figures belonging to the report titled "<strong>Characterization of photorecruitable Guanine Exchange Factors for the study of Rho GTPases in endothelial cells"</strong>. This report was written as part of the Msc programe in Biomedical Science of the University of Amsterdam. </p>
Assessment of endothelial cell function and physiological microcirculatory reserve by video microscopy using a topical acetylcholine and nitroglycerin challenge - Individual subject data
<p>This dataset contains individual subject data for microcirculatory parameters assessed in the present study. Macrocirculatory parameters are not included at this time due to the present study being part of a larger, partially unpublished project.</p>
The bone marrow endothelial progenitor cell response to septic infection: Dataset
<p>An early increase in the level of endothelial progenitor cells (EPCs) in the systemic circulation occurs in patients with septic infection/sepsis. The significance and underlying mechanisms of this response remain unclear. This study investigated the bone marrow EPC response in adult mice with septic infection induced by intravenous injection (i.v.) of <em>Escherichia coli</em>. For <em>in vitro</em> experiments, sorted marrow stem/progenitor cells (SPCs) including lineage(lin)<sup>-</sup>stem cell growth factor receptor(c-kit)<sup>+</sup>stem cell antigen-1(Sca-1)<sup>-</sup>, lin<sup>-</sup>c-kit<sup>+</sup>, and lin<sup>-</sup> cells were cultured with or without lipopolysaccharides (LPS) and recombinant murine vascular endothelial growth factor (VEGF) in the absence and presence of anti-Sca-1 crosslinking antibodies. In a separate set of experiments, marrow lin<sup>-</sup>c-kit<sup>+</sup> cells from green fluorescence protein (GFP)<sup>+</sup> mice, i.v. challenged with heat-inactivated <em>E. coli</em> or saline for 24 h were subcutaneously implanted in Matrigel plugs for 5 weeks. Marrow lin<sup>-</sup>c-kit<sup>+</sup> cells from Sca-1 knockout (KO) mice challenged with heat-inactivated <em>E. coli</em> for 24 h were cultured in the Matrigel medium for 8 weeks. The marrow pool of EPCs bearing the lin<sup>-</sup>c-kit<sup>+</sup>Sca-1<sup>+</sup>VEGF receptor 2 (VEGFR2)<sup>+</sup> (LKS VEGFR2<sup>+</sup>) and LKS CD133<sup>+</sup>VEGFR2<sup>+</sup> surface markers expanded rapidly following septic infection, which was supported by both proliferative activation and phenotypic conversion of marrow stem/progenitor cells. An increase in marrow EPCs and their reprogramming for enhancing angiogenic activity correlated with cell-marked upregulation of Sca-1 expression. Sca-1 coupled with ras-related C3 botulinum toxin substrate 2 (Rac2) in signaling the marrow EPC response. Septic infection caused a substantial increase in plasma levels of IFN-γ, VEGF, G-CSF, and SDF-1. The early increase in circulating EPCs was accompanied by their active homing and incorporation into pulmonary microvasculature. These results demonstrate that the marrow EPC response is a critical component of the host defense system. Sca-1 signaling plays a pivotal role in the regulation of EPC response in mice with septic infection.</p>
Data for: Pericytes' Circadian Clock Affects Endothelial Cells' Synchronization and Angiogenesis in a 3D Tissue Engineered Scaffold
<p>Raw data set and analysis files for Mastrullo et al., Frontiers in Pharmacology, 2022 <strong>DOI:</strong> 10.3389/fphar.2022.867070 </p>
New protein production in primary pulmonary artery endothelial cells treated with insulin-like growth factor 1 treatment
<p>Maximum projections of flat-fielded and deconvolved epi-fluorescence imaging data for primary sheep pulmonary artery endothelial cells (PAEC) treated with vehicle or insulin-like growth factor 1 (IGF1) media.</p> <p>Two cell types: normal PAEC and persistent pulmonary hypertension of the newborn (PPHN) PAEC</p> <p>Three time points: 0 minutes, 1 hour, and 24 hours post-treatment</p> <p>Two fluorescence channels:<br> C0 - DAPI for nuclei (R37606, Life Technologies)<br> C1 - Click-IT new protein translation kit (C10428, C10429, Life Technologies)</p>
Dataset for "Pan-cancer integrative analyses dissect the remodeling of endothelial cells in human cancers"
<p>This is the dataset for "Pan-cancer integrative analyses dissect the remodeling of endothelial cells in human cancers".</p> <p> </p> <p>File "NSR.panE.exprs.all.h5ad.gz" contains processed expression .h5ad data.</p> <p>File "NSR.panE.obs.meta.csv" contains the meta data for this study.</p> <p>File "umap.zip" contains the .csv files for umap coordinates.</p> <p> </p> <p> </p>
Ensemble and single cell data for primary Ovis Aries pulmonary artery endothelial cells response to IGF-1 administration
<p>Data for IGF-1 administration of healthy and persistent pulmonary hypertension of the newborn primary Ovis Aries pulmonary artery endothelial cells.</p> <p>Ensemble.zip:<br> Dose-response to IGF-1<br> Proliferation<br> Western blots for VEGF and eNOS<br> IGF-1R and mTOR inhibition<br> Branch points in tube formation assay<br> <br> IF.zip:<br> Cellprofiler 3.1 analysis of snapshot data for the time course of IGF-1 administration with nuclei, actin, VEGF, and eNOS</p> <p>Translation.zip:<br> CellProfiler 3.1 analysis of snapshot data for the time course of IGF-1 administration with nuclei and total protein production</p>
ROSMAP meQTL Results for Endothelial cells with CpGs
<p>This dataset contains methylation quantitative trait loci (meQTL) results for the following study:</p> <p><strong><em>"regionalpcs improve discovery of DNA methylation associations with complex traits"</em></strong></p> <p>Tiffany Eulalio*<sup>1</sup>, Min Woo Sun<sup>1</sup>, Olivier Gevaert<sup>1</sup>, Michael D. Greicius<sup>2</sup>, Thomas J. Montine<sup>3</sup>, Daniel Nachun*‡<sup>3</sup>, Stephen B. Montgomery*‡<sup>1,3</sup></p> <p>‡ These authors contributed equally as senior authors</p> <p>* Corresponding authors: Tiffany Eulalio (<a href="mailto:eulalio@alumn.stanford.edu">eulalio@alumn.stanford.edu</a>), Daniel Nachun (<a href="mailto:dnachun@stanford.edu">dnachun@stanford.edu</a>), Stephen B. Montgomery (<a href="mailto:smontgom@stanford.edu">smontgom@stanford.edu</a>)</p> <p> Author affiliations:</p> <p>1. Department of Biomedical Data Science, Stanford University, Stanford, CA</p> <p>2. Department of Neurology & Neurological Sciences, Stanford University, Stanford, CA</p> <p>3. Department of Pathology, Stanford University, Stanford, CA</p> <p> </p> <p><strong>Dataset description</strong>:</p> <p>This dataset contains QTL results generated from FastQTL, organized by region type (full gene, gene body, preTSS, and promoters) and summary types (averages and regional principal components).</p> <p><strong>Contents:</strong></p> <ul> <li><strong>Parquet tar files</strong>: These compressed archives contain output files in Parquet format from FastQTL, split by chromosome. <code>parquet1</code> includes chromosomes 1-10, and <code>parquet2</code> includes chromosomes 11-22.</li> <li><strong>cis_qtl_summary_stats.csv</strong>: Provides summary statistics for each phenotype-variant pair, including effect sizes, p-values, TSS distances, and additional details.</li> <li><strong>cis_qtl.signif_pairs.csv</strong>: Contains the significant QTL results identified by FastQTL.</li> <li><strong>cis_qtls.time_to_run.txt</strong>: Reports the running time for FastQTL analysis.</li> <li><strong>cis_qtls.cis_qtl.txt.gz</strong>: Contains comprehensive results for all cis QTLs.</li> </ul> <p>This dataset is intended to support replication and further exploration of QTL associations across different genomic regions and summary methods.</p>
Effect of Statins on Oxidative Stress and Endothelial Progenitor Cells
ClinicalTrials.gov study NCT00166036. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Effect of Saxagliptin and Dapagliflozin on Endothelial Progenitor Cell in Patients With Type 2 Diabetes Mellitus
ClinicalTrials.gov study NCT03660683. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
Effects of Atorvastatin on Endothelial Progenitor Cells After Coronary Surgery
ClinicalTrials.gov study NCT01096875. IPD Sharing: Not stated. Countries: 1. Publications: 1.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.