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124 results for “Endotoxin”
Phyloseq 16S v3v4 object accompanying paper: Host genotype affects endotoxin release in excreta of broilers at slaughter age
<p>This ready to load <strong>phyloseq</strong> R S4 object contains the ASV table, taxonomy table and sample metadata. This data was build using the DaDa2 (version 1.18.0) and phyloseq (version 1.34.0) R packages using the SILVA 138.1 nr99 database and raw MiSeq PE300 sequencing data deposited at NCBI-SRA under BioProject: PRJNA975731.</p> <p>F. Marcato, J.M.J. Rebel, S.K. Kar, I. Wouters, D. Schokker, A. Bossers, F. Harders, J.W. van Riel, M. Wolthuis-Fillerup and I.C. de Jong. </p> <p>The main goal of the current study was to investigate whether or not it was possible to modulate the fecal microbiome and thereby reducing endotoxin concentrations in the excreta of broiler chickens. An experiment was carried out with a 2 x 2 x 2 factorial arrangement including 3 factors, 1) genetic strain (fast-growing Ross 308 vs. slower-growing Hubbard JA757), 2) no vs. combined use of probiotics and prebiotics in the diet and drinking water, 3) early feeding at the hatchery vs. non early feeding. A total of 624 Ross 308 and 624 Hubbard JA757 day-old male broiler chickens were included until d 37 and d 51 of age, respectively. Broilers (26 chicks per pen) were housed in a total of 48 pens, and there were 6 replicate pens/treatment group. Pooled cloacal swabs (10 chickens per pen) for microbiome and endotoxin analyses were collected at a target body weight (BW) of 200 g, 1 kg and 2.5 kg.</p>
Raw Data for the article: Complete intra-laboratory validation of a LAL assay for bacterial endotoxin determination in EBV-specific cytotoxic T lymphocytes
<p>Endotoxin content is a critical factor that affects the safety of biological pharmaceutical products. International pharmacopoeias describe several reference methods to determine endotoxin levels in advanced therapy medicinal product (ATMP) preparations. Administration of ATMPs must be done as rapidly as possible to ensure complete viability and potency of the cellular product. To evaluate the endotoxin content in the shortest time possible, we chose to validate an alternative method based on the use of the Charles River Portable Testing System (PTS) and FDA-approved cartridges, compliant with the requirements of the European Pharmacopoeia and providing results in <20 min. Here, we describe a unique and complete validation approach for instrument, personnel, and analytical method for assessment of endotoxins in ATMP matrices. The PTS system provides high sensitivity and fast quantitative results and uses less raw material and accessories compared with compendial methods. It is also less time consuming and less prone to operator variability. Our validation approach is suitable for a validated laboratory with trained personnel capable of conducting the ATMP release tests, and with very low intra-laboratory variability, and meets the criteria required for an alternative approach to endotoxin detection for in-process and product-release testing of ATMPs.</p>
Mapping the epigenomic landscape of human monocytes following innate immune activation reveals context-specific mechanisms driving endotoxin tolerance
<p><strong>Processed datasets for publication at <em>BMC Genomics</em>.</strong></p> <p>We exposed human primary monocytes from healthy donors (n=6) to interferon-γ or differing combinations of endotoxin (lipopolysaccharide), including acute response (2hr LPS; LPS2) and two models of endotoxin tolerance: repeated stimulations (6+6hr; LPS6:6) and prolonged exposure to endotoxin (24hr LPS; LPS24). Another subset of monocytes was left untreated (naïve; UT). We performed total RNA-seq and ATAC-seq for monocytes across these treatment conditions.</p> <p>The following processed datasets include the raw and normalised count data for each gene or ATAC peak, and the bedGraph and bigWig format for genome-wide signal data. [ bigWig files for RNA-seq (monocytes_*_mean.bw); bigWig files for ATAC-seq (Monocyte_ATAC_*_mean_normalised.by.1/size.factor.bw); bedgraph files for eRNA visualization (*RPKM_FR.bedgraph.gz); raw and normalised count files for ATAC-seq and RNA-seq data (Monocyte.featureCounts_RNAseq.txt.gz; Monocyte_filtered_log2.normalized_RNAseq_counts.txt.gz; Meta.file.txt.gz; Monocyte_raw.counts_ATACseq.txt.gz.).]</p> <p><strong>Data curator:</strong></p> <p>Ping Zhang</p> <p> </p>
Clinical Trial to Assess Pharmacodynamic Effects on Segmental Endotoxin Induced Inflammatory Response of BI 1026706 Versus Placebo
ClinicalTrials.gov study NCT02657408. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Impact of Aspirin Dose Modification on the Innate Immune Response - Will Lower Dose Aspirin Therapy Reduce the Response to Endotoxin
ClinicalTrials.gov study NCT03869268. IPD Sharing: Not stated. Countries: 1. Publications: 36.
Exposure to high endotoxin concentration increases wheezing prevalence among laboratory animal workers: a cross-sectional study
<p><strong>Background:</strong> Endotoxin from Gram-negative bacteria are found in different concentrations in dust and on the ground of laboratories dealing with small animals and animal houses. <strong>Methods: </strong>Cross-sectional study performed in workplaces of two universities. Dust samples were collected from laboratories and animal facilities housing rats, mice, guinea pigs, rabbits or hamsters and analyzed by the “<em>Limulus amebocyte lysate</em>” (LAL) method. We also sampled workplaces without animals. The concentrations of endotoxin detected in the workplaces were tested for association with wheezing in the last 12 months, asthma defined by self-reported diagnosis and asthma confirmed by bronchial hyperresponsiveness (BHR) to mannitol. <strong>Results: </strong>Dust samples were obtained at 145 workplaces, 92 with exposure to animals and 53 with no exposure. Exposed group comprised 412 subjects and non-exposed group comprised 339 subjects. Animal-exposed workplaces had higher concentrations of endotoxin, median of 34.2 endotoxin units (EU) per mg of dust (interquartile range, 12.6-65.4), as compared to the non-exposed group, median of 10.2 EU/mg of dust (interquartile range, 2.6-22.2) (p < 0.001). The high concentration of endotoxin (above median, 20.4 EU/mg) was associated with increased wheezing prevalence (p < 0.001), i.e. 61% of workers exposed to high endotoxin concentration reported wheezing in the last 12 months compared to 29% of workers exposed to low endotoxin concentration. The concentration of endotoxin was not associated with asthma report or with BHR confirmed asthma. <strong>Conclusion: </strong>Exposure to endotoxin is associated with a higher prevalence of wheezing, but not with asthma as defined by the mannitol bronchial challenge test or by self-reported asthma. Preventive measures are necessary for these workers.</p>
Rifaximin in Cirrhosis: Effects on Endotoxin and Haemostatic Indexes
ClinicalTrials.gov study NCT06630572. IPD Sharing: NO. Countries: 1. Publications: 9.
Anti-Inflammatory Effect of Statins in the Human Endotoxin Model
ClinicalTrials.gov study NCT00309374. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Endotoxin Adsorber Hemoperfusion and Microcirculation
ClinicalTrials.gov study NCT01756755. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Peripheral Effects of Endotoxin on Insulin Resistance
ClinicalTrials.gov study NCT00929136. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Endotoxins and Cytokines Removal During Continuous Hemofiltration With oXiris™
ClinicalTrials.gov study NCT03426943. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Cortisol Control of Human Inflammatory Responses to Endotoxin
ClinicalTrials.gov study NCT00396344. IPD Sharing: Not stated. Countries: 1. Publications: 1.
PET Imaging of Endotoxin-induced iNOS Activation
ClinicalTrials.gov study NCT01407796. IPD Sharing: Not stated. Countries: 1. Publications: 1.
S. Endotoxin, Inflammatory Mediators and MRS Before and After Treatment in MHE
ClinicalTrials.gov study NCT01446523. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Endotoxin & Cytokines. Do Protein Loss and Metabolic Effects Depend on Central Nervous System (CNS) Activation of Stress Hormones or on Local Mechanisms in Muscle and Fat?
ClinicalTrials.gov study NCT01452958. IPD Sharing: Not stated. Countries: 1. Publications: 1.
IgM-enriched Immunoglobulin Attenuates Systemic Endotoxin Activity in Early Severe Sepsis
ClinicalTrials.gov study NCT02444871. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Lactate Metabolism After an Endotoxin Challenge in Healthy Humans
ClinicalTrials.gov study NCT01647997. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Study of the Effect of Innate on the Inflammatory Response to Endotoxin
ClinicalTrials.gov study NCT01143480. IPD Sharing: UNDECIDED. Countries: 1. Publications: 3.
Study to Explore the Effects of Probiotics on Endotoxin Levels in Type 2 Diabetes Mellitus Patients
ClinicalTrials.gov study NCT01765517. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Procalcitonin and Endotoxin Sequential Levels to Optimize the Treatment of Bloodstream Infections
ClinicalTrials.gov study NCT00870623. IPD Sharing: Not stated. Countries: 1. Publications: 26.
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