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Fig. 3 in Epidemiology and molecular phylogeny of Babesia sp. in Little Penguins Eudyptula minor in Australia

Fig. 3. Agarose gel electrophoresis of amplification products obtained through nested PCR tests targeting the 18S rRNA gene of Babesia (primers Bab5.1/BabB followed by RLBF/RLBR) or the mitochondrial cytochrome b gene of Haemoproteus/Plasmodium (primers HaemNFI/HaemNR3 followed by HaemF/HaemR2). The following samples are represented: (a) captive-born little penguin chick, negative blood smear; (b) adult wild little penguin, negative blood smear; (c) Babesia-infected adult wild little penguin, as confirmed through blood smear; (d) Haemoproteus-infected adult tropical screech owl, as confirmed through blood smear; (e) Plasmodium-inoculated chicken, raised in arthropod-free environment; (f) blood parasite-free chicken, raised in arthropodfree environment.

Fig. 1 in Epidemiology and molecular phylogeny of Babesia sp. in Little Penguins Eudyptula minor in Australia

Fig. 1. Geographic distribution of sampling locations, southeast Australia. Site details are given in Table 1. The geographic distribution of little penguins (black area) is shown in the top right map (adapted from Marchant and Higgins, 1990).

Fig. 4 in Epidemiology and molecular phylogeny of Babesia sp. in Little Penguins Eudyptula minor in Australia

Fig. 4. Maximum likelihood phylogenetic tree of the 18S rRNA gene of the studied Babesia lineages. Lineages identified in this study are emphasized in red, and other avianinfecting lineages are emphasized in blue. For each lineage, the following information is provided: morphospecies (Genbank ascension number) host species. For avianinfecting lineages, the geographic location is also provided. Branch lengths are drawn proportionally to evolutionary distance (scale bar is shown). For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.

Fig. 2 in Epidemiology and molecular phylogeny of Babesia sp. in Little Penguins Eudyptula minor in Australia

Fig. 2. Babesia sp. in the blood smear of a little penguin. Individual details: TAS- 124, male, adult, moulting, sampled at "Darlington Foreshore" (Maria Island, Tasmania) in 21/02/2013, Genbank ascension number KP144323, Giemsa stain.

Fig. 9 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 9. Similarity of Toxoplasma gondii from Little Penguins with representative strains from Archetypals I, II, and III in the B1 gene. A: shows polymorphisms at the 366 nucleotide. B: shows polymorphisms at the 504 nucleotide. Declaration of competing interest

Fig. 6 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 6. (And detail) – four parasites in a cyst within the cytoplasm of a host cell, spleen (x3810, bar = 2 μm) Image left: E - erythrocyte, Ph - phagocyte, P - protozoa; image right (detail): N - nucleus.

Fig. 5 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 5. Splenic impression smear, erythrocytes (E), splenic stromal cells (S) and numerous protozoa (arrows) (Wright's Giemsa stain, 1000x).

Fig. 4 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 4. Liver, intact and necrotic hepatocytes and numerous protozoa (arrows), free and within cysts (5 μm section, Martius Scarlet Blue stain, 400x). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 11 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 11. Liver, staining of protozoan antigen in intact and necrotic hepatocytes (x400 Toxoplasma polyclonal antibody IHC).

Fig. 3 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 3. Liver, necrotic focus (the area of relative pallor, within which numerous organisms were identifiable) (5 μm section, Haematoxylin and Eosin stain, 100x).

Fig. 10 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 10. Liver, several foci of brown staining indicate Toxoplasma antigen within a necrotic focus (x100, Toxoplasma polyclonal antibody IHC). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 8 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 8. Dividing protozoan forming merozoites (x15000, bar = 1 μm).

Fig. 7 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 7. Protozoan with apical complex (x15000, bar = 1 μm).

Fig. 2 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 2. Enlarged spleen with speckled tan areas of discolouration.

Fig. 1 in Fatal toxoplasmosis in Little Penguins (Eudyptula minor) from Penguin Island, Western Australia

Fig. 1. Enlarged liver with multiple, pinpoint cream to white spots.