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19 results for “FLIM”
Example of Fluorescence Lifetime Imaging Microscopy (FLIM) image stack in .ptu format
<p>The dataset is a 3D stack of fluorescence lifetime imaging microscopy (FLIM) images in ptu format to be used as test and training data. It contains the original .lif file (1) with the stack and a single plane image (to be opened using LAS X and LAS X SMD FLIM), exported raw FLIM data in .ptu format of the stack (3) and the single plane (2a) (to be opened in software capable of reading .ptu files) as well as an intensity image in .tif format (2b) of the single plane for a quick sample overview.</p> <p>The sample is a cross-section of hazel (<em>Corylus avellana</em>) 'diclinous male flower t.s.' with Etzold staining provided by the company Zeiss (CZ 01/05). The dataset was generated using a Leica Stellaris 8 upright confocal laser scanning microscope using a 93x/1.4 glycerol immersion objective. Each image of the 65 slice stack with z step size of 0.287 µm contains 512 x 512 pixels with a pixel size of 0.078 µm x 0.078 µm. Excitation was done with a white-light laser at 491 nm and a laser pulse rate of 40 MHz and a pixel dwell time of 2.0875 µs. Images were acquired using a HyD X detector in counting mode in the spectral range of 496 to 739 nm using Leica Application Suite X (LAS X) version 4.4.0.24861 and LAS X SMD FLIM version 4.5.0 for FLIM image acquisition. 10 frames were accumulated per image. Metadata is available as text file (4a) and as metadata files from LAS X (4b).</p>
FLUTE: a Python GUI for interactive phasor analysis of FLIM data
<p>This repository contains the Fluorescence lifetime imaging microscopy (FLIM) data relative to the following publication <em>"FLUTE: a Python GUI for interactive phasor analysis of FLIM data" </em>https://www.biorxiv.org/content/10.1101/2023.03.31.534529v1</p> <p><em><strong>Fluorescein.tif</strong> </em>stack contains the fluorescence intensity decay of fluorescein solution with a known lifetime of 4ns, used as calibration.</p> <p><strong><em>Embryo.tif</em></strong> file contains the fluorescence intensity decay of a zebrafish embryo at 3 days post fertilisation.</p> <p>Both files have been acquired with the following parameters:</p> <ul> <li>temporal bin number = 56</li> <li>laser repetition rates = 80 MHz</li> <li>bin width = 0.223ns</li> </ul>
Data from: Quantitative single-molecule FLIM and PIE-FRET imaging of biomolecular systems
Open the record for dataset details and reuse information.
Raw data of the publication ''Radical' differences between two FLIM microscopes affect interpretation of cell signaling dynamics'
<p>Raw data and data used to create the figures.</p>
Data to demonstrate : Multiplexed imaging in live cells using pulsed interleaved excitation spectral FLIM
<p>Data to demonstrate :</p> <p>Multiplexed imaging in live cells using pulsed interleaved excitation spectral FLIM - <a href="https://opg.optica.org/oe/fulltext.cfm?uri=oe-32-3-3290&id=545659">https://opg.optica.org/oe/fulltext.cfm?uri=oe-32-3-3290&id=545659</a></p> <p>Trung Duc Nguyen, Yuan-I Chen, Anh-Thu Nguyen, Limin H. Chen, Siem Yonas, Mitchell Litvinov, Yujie He, Yu-An Kuo, Soonwoo Hong, H. Grady Rylander, and Hsin-Chih Yeh, "Multiplexed imaging in live cells using pulsed interleaved excitation spectral FLIM," Opt. Express <strong>32</strong>, 3290-3307 (2024)</p>
FLIM Data and analysis of the hands-on sessions "CF44-1 Label-free metabolic FLIM with 2 photon excitation"
<p>This FLIM dataset on HeLa cells and human astrocytes was acquired during the hands-on sessions <em>CF44-1 Label-free metabolic FLIM with 2 photon excitation</em> during the <a href="https://www.bioimaging.bmc.med.uni-muenchen.de/gerbiflim2024/flimprogram/index.html">German BioImaging </a><a href="https://www.bioimaging.bmc.med.uni-muenchen.de/gerbiflim2024/flimprogram/index.html">workshop on FLIM in Munich</a>.</p> <p>The FLIM data are analyzed with the open source software FLUTE available on GitHub: <a href="https://github.com/LaboratoryOpticsBiosciences/FLUTE"><strong><em>https://github.com/LaboratoryOpticsBiosciences/FLUTE</em></strong></a></p> <p>and published on Biological imaging Journal: <a href="https://www.cambridge.org/core/journals/biological-imaging/article/flute-a-python-gui-for-interactive-phasor-analysis-of-flim-data/862F290EC14187741BDA6B58E9868FA2"><strong><em>Gottlieb, D., Asadipour, B., Kostina, P., Ung, T., & Stringari, C. (2023). FLUTE: A Python GUI for interactive phasor analysis of FLIM data. Biological Imaging, 1-22. doi:10.1017/S2633903X23000211</em></strong></a></p>
Label-free imaging of M1 and M2 macrophage phenotypes in the human dermis in vivo using two-photon excited FLIM
<p>Macrophages (ΜΦs) are important immune effector cells that promote (M1 ΜΦs) or inhibit (M2 ΜΦs) inflammation and are involved in numerous physiological and pathogenic immune responses. Their precise role and relevance, however, are not fully understood for lack of non-invasive quantification methods. Here, we show that two-photon excited fluorescence lifetime imaging (TPE-FLIM), a label-free non-invasive method, can visualize ΜΦs in the human dermis in vivo. We demonstrate in vitro that human dermal ΜΦs exhibit specific TPE-FLIM properties that distinguish them from the main components of the extracellular matrix and other dermal cells. We visualized ΜΦs, their phenotypes and phagocytosis in the skin of healthy individuals in vivo using TPE-FLIM. Additionally, machine learning identified M1 and M2 MФs with a sensitivity of 0.88±0.04 and 0.82±0.03 and a specificity of 0.89±0.03 and 0.90±0.03, respectively. In clinical research, TPE-FLIM can advance the understanding of the role of MФs in health and disease.</p>
Example sdt raw FLIM images of NAD(P)H autofluorescence in Drosophila melanogaster tissues
<p>Raw image files of fluorescence lifetime imaging microscopy (FLIM) of NAD(P)H autofluorescence in unstained living <em>Drosophila melanogaster</em> tissues for image analysis. See <a href="https://www.nature.com/articles/s41598-019-56067-w">publication</a> for technical details. Dataset is derived by reduction of original <a href="http://dx.doi.org/10.25532/OPARA-37">dataset</a> to a single channel and selection of individual images. A single 2 channel sdt FLIM image is included also in which the first channel corresponds to the proper NAD(P)H detection range.</p>
Example of Leica .lif dataset containing FLIM and TileScan data
<p>This dataset contains two files, Intro.lif and Intro.lifext, that contain multiple acquisition datasets from a Leica microscope. Some of the datasets contain metadata about FLIM experiments.</p> <p>Bio-Formats currently reads these datasets incorrectly, it appears as if the offset for the actual pixel data is computed inaccurately, possibly due to the extra metadata present in some series.</p> <p> </p>
Realtime FLIM using napari-live-flim plugin
<p>Realtime FLIM Demonstration using Video and sample files to simulate FLIM-data streaming</p>
First-In-Human Intracoronary OCT-FLIm In Patients Undergoing PCI
ClinicalTrials.gov study NCT04835467. IPD Sharing: NO. Countries: 1. Publications: 3.
Cumulus Cell Mitochondrial Activity as a Non-invasive Marker of Embryo Quality (FLIM)
ClinicalTrials.gov study NCT05332769. IPD Sharing: Not stated. Countries: 1. Publications: 9.
Label-free imaging of M1 and M2 macrophage phenotypes in the human dermis in vivo using two-photon excited FLIM
Open the record for dataset details and reuse information.
Source data and code for publication "Interferometric Excitation FLIM"
Open the record for dataset details and reuse information.
Single-center Clinical Study of Early Diagnosis of Diabetic Cardiomyopathy With FLIM
ClinicalTrials.gov study NCT04534894. IPD Sharing: UNDECIDED. Countries: 0. Publications: 10.
Synthetic TEST FLIM 6D Data Adapted to 5D Modulo
Open the record for dataset details and reuse information.
FLIM data- Studying the PBM effect on HUVEC cells-20220713_HUVEC P 11_Fine piezo_FLD PBM_40MHz
<p>In this experiment, the PBM effects with defined conditions written as the metadata were studied on HUVEC cells under controlled environmental conditions. FLIMs were acquired on control cells, during the PBM illumination and as a time series after the end of PBM.</p>
FLIM data- Studying the PBM effect on HUVEC cells-20220709_HUCVEC P 11_fine piezo_40MHz
<p>In this experiment, the PBM effects with defined conditions written as the metadata were studied on HUVEC cells under controlled environmental conditions. FLIMs were acquired on control cells, during the PBM illumination and as a time series after the end of PBM.</p>
Real-time Diagnosis and Visualization of Tumor Margins in Excised Breast Specimens Using Zenith FLIM Diagnostics
ClinicalTrials.gov study NCT04683120. IPD Sharing: Not stated. Countries: 1. Publications: 0.
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.