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15
datasets available to search
ShareScore release 0.9.0
Dataset results
15 results for “FUCCI”
Fluorescence time-lapse images of MDA-MB-231 cells expressing FUCCI(CA)2 (Part 1/2)
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Single cell FUCCI tracking data
<p>These datasets contain single cell FUCCI tracking data for the data analysis R scripts in the FUCCI_analysis folder in the heldring-E2S-modeling GitHub repository available via the persistent link https://doi.org/10.5281/zenodo.8143692.</p>
Fluorescence time-lapse images of MDA-MB-231 cells expressing FUCCI(CA)2 (Part 2/2)
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Live-cell imaging data set of HaCaT FUCCI cells with BF/DIC and fluorescence
<p>This dataset contains time-lapse acquisitions of HaCaT FUCCI cells cultured in 8-well chambered coverslips with fluorobrite media. Cells were provided by Dr. Francesco Pasqualini and Dr. Moises di Sante (<a title="http://www.syntheticphysiologylab.com/" href="http://www.syntheticphysiologylab.com/" target="_blank" rel="noreferrer noopener">http://www.syntheticphysiologylab.com</a>), and videos acquired at the Henriques Lab (<a title="https://henriqueslab.org/" href="https://henriqueslab.org/" target="_blank" rel="noreferrer noopener">https://henriqueslab.org/</a>). </p> <p>HaCaT_FUCCI_BF-live set: Nikon Ti2, Channels BF (16 bit), FITC (12 bit sensitive) and Cy5 (12 bit sensitive). 20 positions, acquired in Nikon Ti2 with a 20x/0.8, every 30 minutes for 18 hours. </p> <p><span>HaCaT_FUCCI_DIC-live set: Nikon Ti2, Channels DIC, FITC and Cy5 (all 12 bit sensitive). 20 positions, acquired in Nikon Ti2 with a 20x/0.8, every 30 minutes for 18 hours. </span></p>
Single-Cell Imaging Dataset: Hela FUCCI Cell Fluorescence Analysis
<p>Hela FUCCI Cell Dataset: Fluorescence Intensity and Segmentation</p> <p>The "Hela FUCCI Cell Dataset" is a comprehensive collection of fluorescence microscopy data capturing the fluorescence intensity of Hela FUCCI cells. The dataset encompasses a diverse range of cellular images acquired through fluorescence imaging techniques, offering valuable insights into the cellular behavior and fluorescent signal patterns.</p> <p>Contents:</p> <p>Fluorescence Intensity Data: The dataset includes fluorescence images of Hela FUCCI cells captured in both red and green channels. These images represent the intensity levels of cellular fluorescence signals.</p> <p>Purpose:<br> The dataset serves as a resource for researchers and scientists interested in cellular fluorescence analysis. It supports investigations into cellular dynamics, cell cycle studies, and fluorescence signal patterns. Researchers can utilize this dataset to develop and evaluate image processing, analysis, and machine learning techniques for cell detection and fluorescence quantification.</p> <p>Data Collection:<br> The data were collected using fluorescence microscopy techniques, capturing the distinct fluorescence signals emitted by Hela FUCCI cells. </p> <p>Usage:<br> Researchers can use this dataset to:</p> <p>Investigate fluorescence patterns and intensities of Hela FUCCI cells.<br> Develop and validate machine learning algorithms for cell segmentation and detection.<br> Explore cellular behaviors and dynamics under various experimental conditions.</p> <p>Citation:<br> If you use this dataset in your research, please cite the original source to acknowledge its contribution.</p> <p>Access and Availability:<br> The dataset is openly available through Zendo, accessible via the following link: https://zenodo.org/. Researchers are encouraged to explore, analyze, and contribute to the dataset's applications and advancements in cellular fluorescence analysis.</p>
PIP-FUCCI
<p>These are accompanying files to the CellMAPtracer software (<a href="https://doi.org/10.5281/zenodo.3878088">https://doi.org/10.5281/zenodo.3878088</a>) and manuscript (under review). Biosensor described in <a href="https://www.tandfonline.com/doi/full/10.1080/15384101.2018.1547001">https://www.tandfonline.com/doi/full/10.1080/15384101.2018.1547001</a>.</p>
Data from: Screening of cytotoxic or cytostatic flavonoids with quantitative FUCCI-based cell cycle assay
The Fluorescence-Ubiquitin Cell Cycle Indicator (FUCCI) system can be used not only to study gene expression at a specific cell cycle stage, but also to monitor cell cycle transitions in real time. In this study, we used a single clone of FUCCI-expressing HeLa cells (FUCCI-HeLa cells) and monitored the cell cycle in individual live cells over time by determining the ratios between red fluorescence (RF) of RFP-Cdt1 and green fluorescence (GF) of GFP-Geminin. Cytotoxic and cytostatic compounds, the latter of which induced G2 or mitotic arrest, were identified based on periodic cycling of the RF/GF and GF/RF ratios in FUCCI-HeLa cells treated with anticancer drugs. With this cell cycle monitoring system, ten flavonoids were screened. Of these, apigenin and luteolin, which have a flavone backbone, were cytotoxic, whereas kaempferol, which has a flavonol backbone, was cytostatic and induced G2 arrest. In summary, we developed a system to quantitatively monitor the cell cycle in real time. This system can be used to identify novel compounds that modulate the cell cycle and to investigate structure–activity relationships.
Data from: Screening of cytotoxic or cytostatic flavonoids with quantitative FUCCI-based cell cycle assay
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CellMAPtracer-FUCCI-2channels TIFF files
<p>These are accompanying files to the <a href="https://doi.org/10.5281/zenodo.3878088">CellMAPtracer software</a> and manuscript (under review).</p>
Sequencing of metabolically labeled transcripts in single cells from RPE1-FUCCI cells and murine intestinal organoids.
GEO Series GSE128365. Mus musculus; Homo sapiens. 80 samples. Type: Expression profiling by high throughput sequencing; Other.
HCT 116 cells expressing Fucci (fluorescent ubiquitination-based cell cycle indicator); G1 phase vs. S/G2/M phase
GEO Series GSE34940. Homo sapiens. 4 samples. Type: Expression profiling by array.
DNA damage response in single RPE-FUCCI cells
GEO Series GSE146759. Homo sapiens. 4 samples. Type: Expression profiling by high throughput sequencing.
RNA-seq analysis evaluating cell cycle specific gene expression and the effect of KRTAP2-3 gene inactivation in SAS cells expressing Fucci.
GEO Series GSE179223. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Bulk RNAseq NIEHS P30 CURES Pilot Environmental hormonal and mutagenic stress in fluorescent-ubiquitinated cell cycle indicator (FUCCI) mESC 2021
GEO Series GSE196827. Mus musculus. 36 samples. Type: Expression profiling by high throughput sequencing.
Cell cycle analysis of histone marks and 4C in human Fucci ESCs
GEO Series GSE61176. Homo sapiens. 28 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Other.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.