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Dataset results
16 results for “Fecal DNA”
Data from: Non-invasive age estimation based on fecal DNA using methylation-sensitive high-resolution melting for Indo-Pacific bottlenose dolphins
<p class="MsoNormal"><span>Age is necessary information for the study of life history of wild animals. A general method to estimate the age of odontocetes is counting dental growth layer groups (GLGs). However, this method is highly invasive as it requires the capture and handling of individuals to collect their teeth.</span><span> Recently, the development of DNA-based age </span><span>estimation methods has been actively studied as an alternative to such invasive methods, of which many have used biopsy samples. However, if DNA-based age estimation can be developed from fecal samples, age estimation can be performed without touching or disrupting individuals, thus establishing an entirely non-invasive method. </span><span>We developed an age estimation model using the methylation rate of two gene regions, <em>GRIA2</em> and <em>CDKN2A,</em> measured through methylation-sensitive high-resolution melting (MS-HRM) from fecal samples of wild Indo-Pacific bottlenose dolphins (<em>Tursiops aduncus</em>). The age of individuals was known through conducting longitudinal individual identification surveys underwater. Methylation rates were quantified from 36 samples. Both gene regions showed a significant correlation between age and methylation rate. The age estimation model was constructed based on the methylation rates of both genes which achieved sufficient accuracy (after LOOCV: MAE = 5.08, <em>R<sup>2</sup></em> = 0.34) for the ecological studies of the Indo-Pacific bottlenose dolphins, with a lifespan of 40-50 years. This is the first study to report the use of non-invasive fecal samples to estimate the age of marine mammals.</span></p>
Data from: Non-invasive age estimation based on fecal DNA using methylation-sensitive high-resolution melting for Indo-Pacific bottlenose dolphins
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Canis lupus fecal DNA dataset
<p>Sea otters (<em>Enhydra</em> <em>lutris</em>) and wolves (<em>Canis</em> <em>lupus</em>) are two apex predators with strong and cascading effects on ecosystem structure and function. After decades of recovery from near extirpation, their ranges now overlap, allowing sea otters and wolves to interact for the first time in the scientific record. We intensively studied wolves during 2015–2021 in an island system colonized by sea otters in the 2000s and by wolves in 2013. After wolf colonization, we quantified shifts in foraging behavior with DNA metabarcoding of 689 wolf scats and stable isotope analyses, both revealing a dietary switch from Sitka black-tailed deer (<em>Odocoileus</em> <em>hemionus</em>), the terrestrial in situ primary prey, to sea otters. Here we show an unexpected result of the reintroduction and restoration of sea otters, which became an abundant marine subsidy for wolves following population recovery. The availability of sea otters allowed wolves to persist and continue to reproduce, subsequently nearly eliminating deer. Genotypes from 390 wolf scats and telemetry data from 13 wolves confirmed island fidelity constituting one of the highest known wolf population densities and upending standardly accepted wolf density predictions based on ungulate abundance. Whereas marine subsidies in other systems are generally derived from lower trophic levels, here an apex nearshore predator became a key prey species and linked nearshore and terrestrial food webs in a recently deglaciated and rapidly changing ecosystem. These results underscore that species restoration may serve as an unanticipated nutrient pathway for recipient ecosystems even resulting in cross-boundary subsidy cascades.</p>
Fecal DNA metabarcoding-derived diets of deer in Sweden
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Canis lupus fecal DNA dataset
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Data from: Noninvasive individual and species identification of jaguars (Panthera onca), pumas (Puma concolor) and ocelots (Leopardus pardalis) in Belize, Central America using cross-species microsatellites and fecal DNA
There is a great need to develop efficient, noninvasive genetic sampling methods to study wild populations of multiple, co-occurring, threatened felids. This is especially important for molecular scatology studies occurring in challenging tropical environments where DNA degrades quickly and the quality of faecal samples varies greatly. We optimized 14 polymorphic microsatellite loci for jaguars (Panthera onca), pumas (Puma concolor) and ocelots (Leopardus pardalis) and assessed their utility for cross-species amplification. Additionally, we tested their reliability for species and individual identification using DNA from faeces of wild felids detected by a scat detector dog across Belize in Central America. All microsatellite loci were successfully amplified in the three target species, were polymorphic with average expected heterozygosities of HE = 0.60 ± 0.18 (SD) for jaguars, HE = 0.65 ± 0.21 (SD) for pumas and HE = 0.70 ± 0.13 (SD) for ocelots and had an overall PCR amplification success of 61%. We used this nuclear DNA primer set to successfully identify species and individuals from 49% of 1053 field-collected scat samples. This set of optimized microsatellite multiplexes represents a powerful tool for future efforts to conduct noninvasive studies on multiple, wild Neotropical felids.
Data from: Relatedness and demography of African forest elephants: inferences from noninvasive fecal DNA analyses
African forest elephants (Loxodonta cyclotis) are genetically and morphologically distinct from their savannah counterparts, but their biology remains poorly understood. In this study, I use noninvasive fecal DNA analyses to examine the relatedness structure and historical demography of forest elephants at two sites in SW Gabon, central Africa. Pairwise relatedness values calculated between 162 elephant individuals genotyped at eight microsatellite loci were significantly higher within spatially associated dung piles than between random pairings for one site. First- and second-order relatives were most commonly detected among dung piles from adult female pairs and adult females and juveniles. Pairwise relatedness estimates suggested that, like savannah elephants, forest groups are largely composed of adult females, their sisters, and juvenile offspring. Associations between males, and groups containing juveniles from multiple related females, were detected but at much lower frequency. Analysis of mitochondrial d-loop sequences from 70 elephant individuals identified two haplogroups in SW Gabon.
Establishment of a fecal DNA quantification technique for rare and cryptic diet constituents in small mammals - raw data
<p class="MsoNormal"><span>DNA-based approaches have highly improved the applicability of dietary studies aimed at investigating ecological processes. These studies have provided direct insights into, otherwise difficult to measure, interactions between species and trophic levels, food web structure and ecosystem functioning. However, despite these advances, DNA-based methods have been struggling to accurately quantify the whole breadth of diet constituents because of methodological biases, such as amplification bias and digestive processes. This study is, to our knowledge, the first diet study that used droplet digital PCR to quantify diet constituents. We manipulated the diet of wild caught wood mice (<em>Apodemus sylvaticus</em>) by feeding them with a known amount of small vegetable seeds (onion and carrot) and quantified the DNA traces of these diet constituents in fecal samples. The sensitivity of the technique combined with the control on the experimental design allowed mitigation of methodological bias. We were able to accurately determine DNA concentrations of small vegetable seeds in the diet of wood mice. Quantification of target DNA demonstrated significant differences in DNA content when one vs. five seeds were consumed. <a name="_Hlk96526385"></a>These differences remained significant when the age, sex, and other diet constituents of the mice were altered. Different DNA markers, targeting different parts of the chloroplast, influenced onion DNA detectability. However, all onion and carrot markers showed higher DNA content for higher seed numbers. Overall, the sensitive DNA based approach developed in this study allows for minimally-invasive quantification of small diet constituents in feces, which would otherwise be undetectable with traditional methods.</span></p>
Circulating-free DNA Assessment as a Tool to Predict Colorectal Cancer in Subjects With a Positive Fecal Immunoassay.
ClinicalTrials.gov study NCT03981679. IPD Sharing: NO. Countries: 1. Publications: 5.
Data from: Relatedness and demography of African forest elephants: inferences from noninvasive fecal DNA analyses
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Data from: Noninvasive individual and species identification of jaguars (Panthera onca), pumas (Puma concolor) and ocelots (Leopardus pardalis) in Belize, Central America using cross-species microsatellites and fecal DNA
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Establishment of a fecal DNA quantification technique for rare and cryptic diet constituents in small mammals - raw data
Open the record for dataset details and reuse information.
Fecal DNA Methylation Detection for Gastric Cancer
ClinicalTrials.gov study NCT06943807. IPD Sharing: UNDECIDED. Countries: 0. Publications: 0.
Fecal DNA Methylation Test for Colorectal Cancer Screening
ClinicalTrials.gov study NCT04823793. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Fecal DNA Methylation and Helicobacter Pylori SNPs Tests for Gastric Cancer
ClinicalTrials.gov study NCT07312500. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Fecal DNA Methylation and Helicobacter Pylori Gastric Cancer Susceptibility Genes Test
ClinicalTrials.gov study NCT06943768. IPD Sharing: Not stated. Countries: 0. Publications: 0.
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Allen Brain Atlas
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International Brain Laboratory public data
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OpenNeuro
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