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Effect of the overexpression of the GGP1 gene on cell wall remodelling and redox state in the tomato fruit

The deposited data were collected as a part of the studies entitled ‘Effect of the overexpression of the GGP1 gene on cell wall remodelling and redox state in the tomato fruit’. The research is the result of cooperation between institutions:  <ul> <li>Group for Plant Molecular Biology, Institute of Molecular Genetics and Genetic Engineering (IMGGE) at the University of Belgrade (Serbia),</li> <li>Institute of Agrophysics, Polish Academy of Sciences (Poland),</li> <li>Department of Pharmaceutical Sciences, at the Aristotle University of Thessaloniki (Greece).</li> </ul> The use of advanced microscopic techniques (immunolabeling method), methods of molecular biology (Western blotting), and biochemistry (HPLC, measurement of enzyme activities) allows for expanding knowledge in the field of plant cell physiology and horticulture.   The attached files have been compressed to *.zip format. The dataset consists of the following files: A_1_Antioxidant enzymes activities A_2_CLSM imaging A_3_Native polyacrylamide electrophoresis of antioxidant enzymes A_4_Phenolic components in the fruit tissue A_5_Western Blotting with quantitative analysis

Fig. 3 in Overexpression of DsEXLA2 gene from Dendrocalamus sinicus accelerates the plant growth rate of Arabidopsis

Fig. 3. Phylogenetic tree of expansin-like A genes from different plant species. Numbers such as 100, 99 and 97 represent the percentage bootstrap values. The accession numbers corresponding to expansin-like A proteins are as listed in Supplemental Table S2.

Fig. 8 in Overexpression of DsEXLA2 gene from Dendrocalamus sinicus accelerates the plant growth rate of Arabidopsis

Fig. 8. Effect of DsEXLA2 on cell wall thickness. A: Micrographs of stem vessel. B: Cellulose content. C. Cell wall thickness. Line 3, 9 and 10 represent the three independent transgenic lines selected. *p <0.05; **p <0.01 (Student's t-test). Bars represent ST. The scale represents 50 μm.

Fig. 7 in Overexpression of DsEXLA2 gene from Dendrocalamus sinicus accelerates the plant growth rate of Arabidopsis

Fig. 7. Changes of plant height at different growth stages. * represents the differences between the transgenic plants and Col-0 at the same growth stage at p ≤ 0.05 according to the Duncan test. Bars represent SE. Line 3, 9 and 10 represent the three independent transgenic lines selected.

Fig. 2 in Overexpression of DsEXLA2 gene from Dendrocalamus sinicus accelerates the plant growth rate of Arabidopsis

Fig. 2. Motif distribution in the EXLA2 of different plant species. A: Motifs investigated with the MEME web server. Different motifs are represented in different colors. B: Detailed information on each motif. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Overexpression of DsEXLA2 gene from Dendrocalamus sinicus accelerates the plant growth rate of Arabidopsis

Fig. 1. Sequence alignment of the EXLA2 from different plant species. Blue box indicates signal peptide. Red box indicates the CDRC motif. Asterisk indicates cysteine. Plus indicates tryptophan. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Fig. 10 in Overexpression of DsEXLA2 gene from Dendrocalamus sinicus accelerates the plant growth rate of Arabidopsis

Fig. 10. The expression patterns of 20 selected DEGs compared for RNA-seq and qRT-PCR. *p <0.05 based on Student's t-test. Bars represent SE; n = 3. EXLA2: Expansin-like A2; EXLA3: Expansin-like A3; EXLB1: Expansin-like B1; EXPB3: Expansin-B3; COBL8: COBRA-like protein 8; CSLD3: Cellulose synthase-like protein D3; CCR2: Cinnamoyl-CoA reductase 2; XTH11: Xyloglucan endotransglucosylase/hydrolase protein 11; RRTF1: Redox responsive transcription factor 1; LOG5: Lonely guy 5; CYP707A3: Cytochrome P450 707A3; GA2OX6: gibberellin 2-oxidase 6; WRKY40: WRKY DNA-binding protein 40; SUS3: Sucrose synthase 3; LOX3: Lipoxygenase 3; TAT3: Tyrosine aminotransferase 3; FBA5: Fructose-bisphosphate aldolase 5; LHCB2.3: Photosystem II light-harvesting complex gene 2.3; LHB1B1: Light-harvesting chlorophyll-protein complex II subunit B1; RBCS2B: Ribulose bisphosphate carboxylase small chain 2B.

Fig. 5 in Overexpression of DsEXLA2 gene from Dendrocalamus sinicus accelerates the plant growth rate of Arabidopsis

Fig. 5. Subcellular localization of DsEXLA2 in the epidermal leaf cells of tobacco. 35S-YFP and 35S::DsEXLA2-YFP fusion protein were transiently expressed in tobacco, respectively. The 35S-YFP vector was used as a negative control.

Fig. 9 in Overexpression of DsEXLA2 gene from Dendrocalamus sinicus accelerates the plant growth rate of Arabidopsis

Fig. 9. RNA-seq analysis of 35S::DsEXLA2 transgenic plants. A: Volcano map of DEGs. B: Expression level of expansin–like family genes. C: Enrichment bubble map of KEGG_pathway in up-regulated genes. D: Enrichment bubble map of KEGG_pathway in down-regulated genes.

Fig. 4 in Overexpression of DsEXLA2 gene from Dendrocalamus sinicus accelerates the plant growth rate of Arabidopsis

Fig. 4. Expression analysis of DsEXLA2 in D. sinicus. A: Sample schematic of culm. B: qRT-PCR analysis of the DsEXLA2 gene expression in different internodes. C: qRT-PCR analysis the DsEXLA2 gene expression in different tissues. D: sqRT-PCR analysis of the DsEXLA2 gene expression in different internodes. E: sqRT-PCR analysis the DsEXLA2 gene expression in different tissues. Different letters in the column line indicate differences at p <0.05 based on the Duncan test, respectively. Bars represent ST.

Fig. 6 in Enhancement of antroquinonol production via the overexpression of 4-hydroxybenzoate polyprenyltransferase biosynthesis-related genes in Antrodia cinnamomea

Fig. 6. Time profiles of the contents of AQ (A) and biomass (B) in the pCT74- gpd, pCT74-gpd-ubiA, and pCT74-gpd-CoQ2 transformants under submerged culture condition. a-c Different lower-case letters indicate a significant difference (p <0.05).

Fig. 4 in Enhancement of antroquinonol production via the overexpression of 4-hydroxybenzoate polyprenyltransferase biosynthesis-related genes in Antrodia cinnamomea

Fig. 4. The relative mRNA expression levels of the ubiA (A) and CoQ2 (B) genes. Expression levels in the pCT74-gpd strain samples are defined as 1.0, and expression levels in the transgenic strain are displayed as the fold increase over the reference sample. a-e Different lower-case letters indicate a significant difference (p <0.05).

Fig. 5 in Enhancement of antroquinonol production via the overexpression of 4-hydroxybenzoate polyprenyltransferase biosynthesis-related genes in Antrodia cinnamomea

Fig. 5. The HPLC chromatogram of the 95% ethanol-extracted compounds from pCT74-gpd (A), pCT74-gpd-ubiA (B), and pCT74-gpd-CoQ2 (C) transformants. The culture experiments were carried out in 500-mL shake flasks at 28 ◦C and 150 rpm for 10 days.

Fig. 3 in Enhancement of antroquinonol production via the overexpression of 4-hydroxybenzoate polyprenyltransferase biosynthesis-related genes in Antrodia cinnamomea

Fig. 3. Growth morphology of A. cinnamomea and its transformants on PDA at 12 days. A, Wild-type A. cinnamomea S-29 cultivated on PDA plate; B, C and D, Transformants cultivated on selective PDA plates (B, pCT74-gpd; C, pCT74-gpd-ubiA; D, pCT74-gpd-CoQ2).

Fig. 2 in Enhancement of antroquinonol production via the overexpression of 4-hydroxybenzoate polyprenyltransferase biosynthesis-related genes in Antrodia cinnamomea

Fig. 2. The construction of three plasmids (A) and verification of transformants (B). Lane M, DNA marker; Lane 1, the pCT74-gpd plasmid as a positive control; Lane 2, the pCT74-gpd transformant; Lane 3, the pCT74-gpd-ubiA plasmid as a positive control; Lane 4, the pCT74-gpd-ubiA transformant; Lane 5, the pCT74-gpd-CoQ2 plasmid as a positive control; Lane 6, the pCT74-gpd-CoQ2 transformant; Lane WT, the wild-type strain; Lane N, negative control.

Fig. 1. A in Enhancement of antroquinonol production via the overexpression of 4-hydroxybenzoate polyprenyltransferase biosynthesis-related genes in Antrodia cinnamomea

Fig. 1. A. Effect of digestion time on protoplast yield; B. Effect of enzyme concentration on protoplast yield. a-f Different lower-case letters indicate a significant difference (p <0.05).