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datasets available to search
ShareScore release 0.9.0
Dataset results
19 results for “Genetic interference”
Data from: Reverse genetics in the tidepool: knockdown of target gene expression via RNA interference in the copepod Tigriopus californicus
Reverse genetic tools are essential for characterizing phenotypes of novel genes and testing functional hypotheses generated from next-generation sequencing studies. RNA interference (RNAi) has been a widely used technique for describing or quantifying physiological, developmental or behavioural roles of target genes by suppressing their expression. The marine intertidal copepod Tigriopus californicus has become an emerging model for evolutionary and physiological studies, but this species is not amenable to most genetic manipulation approaches. As crustaceans are susceptible to RNAi-mediated gene knock-down, we developed a simple method for delivery of gene-specific double-stranded RNA that results in significant suppression of target gene transcription levels. The protocol was examined on five genes of interest, and for each, at least 50% knock-down in expression was achieved. While knock-down levels did not reach 100% in any trial, a well-controlled experiment with one heat-shock gene showed unambiguously that such partial gene suppression may cause dramatic changes in phenotype. Copepods with suppressed expression of heat-shock protein beta 1 (hspb1) exhibited dramatically decreased tolerance to high temperatures, validating the importance of this gene during thermal stress, as proposed by a previous study. The application of this RNAi protocol in T. californicus will be invaluable for examining the role of genes putatively involved in reproductive isolation, mitochondrial function and local adaptation.
Data from: Reverse genetics in the tidepool: knockdown of target gene expression via RNA interference in the copepod Tigriopus californicus
Open the record for dataset details and reuse information.
A Genetic Algorithm Optimization for Fracture Length to Prevent Subsurface Interference during drilled cuttings reinjection Files
<p>The files associated with the publication.</p>
U937 cells with CRISPR interference at regulatory genetic variants [ATAC-seq]
GEO Series GSE281908. Homo sapiens. 6 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Data from: Does a coexisting congener affect the genetic structure and selfing rate via reproductive interference?
<p>Data archived here were used for analyses in Katsuhara et al. "Does a coexisting congener affect the genetic structure and selfing rate via reproductive interference?" Data is in two worksheets.</p>
Serotonin, Serotonin Genetics(TPH2) and Emotion and Interference Processing
ClinicalTrials.gov study NCT03549182. IPD Sharing: NO. Countries: 1. Publications: 0.
An inducible CRISPR-interference library for genetic interrogation of Saccharomyces cerevisiae biology
GEO Series GSE159409. Saccharomyces cerevisiae; Escherichia coli. 20 samples. Type: Other.
Parallel quantitative CRISPR interference in yeast identifies chemical-genetic interactions and new rules for guide RNA design
GEO Series GSE71490. Saccharomyces cerevisiae. 84 samples. Type: Other.
U937 cells with CRISPR interference at regulatory genetic variants [4C-seq]
GEO Series GSE281909. Homo sapiens. 6 samples. Type: Other.
Genetic screen for suppressors of lncRNA-mediated transcription interference identifies a gain-of-function mutation in the essential Pol2 termination factor Seb1
GEO Series GSE168898. Schizosaccharomyces pombe. 6 samples. Type: Expression profiling by high throughput sequencing.
Genetic activation of canonical RNA interference in mice
GEO Series GSE242871. Mus musculus. 78 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Genetic screen for suppression of transcriptional interference reveals fission yeast 14-3-3 protein Rad24 as an antagonist of precocious Pol2 transcription termination
GEO Series GSE164616. Schizosaccharomyces pombe. 6 samples. Type: Expression profiling by high throughput sequencing.
Genetic activation of canonical RNA interference in mice [spleen]
GEO Series GSE242869. Mus musculus. 15 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Genetic activation of canonical RNA interference in mice [thymus]
GEO Series GSE242870. Mus musculus. 12 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Genetic activation of canonical RNA interference in mice [skeletal muscle]
GEO Series GSE259395. Mus musculus. 9 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Genetic activation of canonical RNA interference in mice [heart]
GEO Series GSE242867. Mus musculus. 15 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Genetic activation of canonical RNA interference in mice [liver]
GEO Series GSE242868. Mus musculus. 15 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Genetic activation of canonical RNA interference in mice [brain]
GEO Series GSE242866. Mus musculus. 12 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Efficacy of small molecule inhibitors and genetic interference of cGAS-STING axis on development of the senescence-associated secretory phenotype (SASP) in human stromal cells
GEO Series GSE128420. Homo sapiens. 18 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.