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2 results for “Genome in a Bottle”
Sequencing Genome in a Bottle samples
<p>We are pleased to announce the release of a new addition to the Oxford Nanopore Open Data project: sequencing of several Genome in a Bottle samples (including the Ashkenazi Trio). Sequencing was performed with the 5 kHz upgrade to the <a href="https://store.nanoporetech.com/uk/ligation-sequencing-kit-v14.html">Ligation Sequencing Kit V14</a> released in MinKNOW 23.04.05. As such the quality of data presented here should be representative of routine sequencing that can be performed by any lab using this latest release.</p> <p>These reference samples were sequenced with two PromethION flow cells each to yield around more than 200 Gbases sequencing data per sample.</p> <blockquote> <p>The following cell line samples were obtained from the NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research: GM12878, GM24143, GM24149, GM24385</p> </blockquote> <p><em>Data location</em></p> <p>As with previous releases the new dataset is available for anonymous download from an Amazon Web Services S3 bucket. The bucket is part of the <a href="https://aws.amazon.com/opendata/">Open Data on AWS</a> project enabling sharing and analysis of a wide range of data.</p> <p>The data is located in the bucket at:</p> <blockquote> <pre>s3://ont-open-data/giab_2023.05/</pre> </blockquote> <p>See the <a href="https://labs.epi2me.io/tutorials/">tutorials</a> page for information on downloading the dataset.</p> <p><em>Sequencing Outputs</em></p> <p>Two flowcells were used to sequence each of the samples to high depth:</p> <table> <thead> <tr> <th>Genome</th> <th>Description</th> <th>Cell line</th> </tr> </thead> <tbody> <tr> <td>HG001</td> <td>CEPH/UTAH</td> <td>GM12878</td> </tr> <tr> <td>HG002</td> <td>PGP Ashkenazi Son</td> <td>GM24385</td> </tr> <tr> <td>HG003</td> <td>PGP Ashkenazi Father</td> <td>GM24149</td> </tr> <tr> <td>HG004</td> <td>PGP Ashkenazi Mother</td> <td>GM24143</td> </tr> </tbody> </table> <p>For each flowcell used in the sequencing the PromethION device outputs are available. All data is present as <code>.pod</code> files, along with associated summary files in a structured fashion.</p> <p><em>Further Information</em></p> <ul> <li><em>https://labs.epi2me.io/giab-2023.05</em></li> </ul>
Genome in a Bottle Direct-RNA Sequencing: GM24631 Calibration-Strand Aligned Reads
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