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95 results for “Glucosinolates”
Glucosinolate and Desulfoglucosinolate NMR Data and Structures
<p><sup>1</sup>H, <sup>13</sup>C, <sup>15</sup>N NMR spectra, NMR parameters, and structures of a set of 31 glucosinolates or desulfoglucosinolates</p>
Functions of the Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host glucosinolates adaptation of Helicoverpa armigera
<p>In this study, we detailed the indicators of development of <em>H. armigera</em>, such as growth and development period, number of surviving larvae, body weight, pupa weight, and number, as well as the relative expression levels of <em>HaSumf1</em> gene at different instars, and the third- and fifth-instars after feeding with glucosinolates and /or ds<em>Sumf1</em>.</p>
Data from: Soil variation among natural habitats alters glucosinolate content in a wild perennial mustard
<p>The archive Wagner_Mitchell-Olds_Zenodo_SoilPlast.zip contains a README file describing the contents.</p> <p>These data are described in the Journal of Experimental Botany: <a href="https://doi.org/10.1093/jxb/erac520">https://doi.org/10.1093/jxb/erac520</a></p> <p>Funding: National Science Foundation, IOS-<span>2016351</span></p>
Bivariate GWA mapping reveals associations between aliphatic glucosinolates and plant responses to thrips and heat stress
<p>Supplemental data on bivariate GWA mapping of stress phenotypes and metabolomes of Arabidopsis.</p>
Functions of the Sulfatase-Modifying Factor 1 (HaSumf1) in the development and host glucosinolates adaptation of Helicoverpa armigera
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Data from: Testing the optimal defense hypothesis in nature: variation for glucosinolate profiles within plants
Open the record for dataset details and reuse information.
Fig. 2 in Comparison of glucosinolate diversity in the crucifer tribe Cardamineae and the remaining order Brassicales highlights repetitive evolutionary loss and gain of biosynthetic steps
Fig. 2. The essence of glucosinolate (GSL) biosynthesis as imagined for the presumably ancient 2-methylpropylGSL and a β-hydroxylated derivative, 2-hydroxy-2-methylpropylGSL. The three steps between the CYP83 product and thiohydroximic acid in general GSL biosynthesis involves glutathione, serving as the donor of sulfur. The illustrated hypothetic pathway is based on the known biosynthetic pathway of more recently evolved GSLs (Sønderby et al., 2010).
Fig. 8 in Comparison of glucosinolate diversity in the crucifer tribe Cardamineae and the remaining order Brassicales highlights repetitive evolutionary loss and gain of biosynthetic steps
Fig. 8. Biochemical aspects of aliphatic side chain oxidation of glucosinolates (GSLs). A. Biosynthesis of three well-investigated GSLs, all involving enzymes of the class "2-oxoglutarate-dependent dioxygenases", although the case of BAR biosynthesis is still tentative (Byrne et al., 2017). B. Conserved metabolism of an OAT into the corresponding oxazolidine-2-one (OAO) in three Brassicales species (Barbarea vulgaris, Nasturtium officinale and Reseda luteola). MYR, myrosinase; GS-OH, glucosinolate hydroxylating enzyme; GRS, glucoraphasatin synthase.
Fig. 7 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 7. Levels of major glucosinolates in leaves of first year rosette plants of the Gtype (A) and P-type (B) of Barbarea vulgaris in plants subjected to various challenges or no challenge as control. The contrasting general profile of the types is evident from dominance of BAR in the G-type and EBAR in the P-type. Treatment codes are: Control, un-challenged plants harvested after 7 days; Pieris 3d and Pieris 7d, herbivory by Pieris brassicae larvae until harvest at either day 3 or day 7; Plutella, herbivory by Plutella xylostella for 4 days; CuCl2, spraying of leaves with 10 mM CuCl2 (aq.) followed by recovery for 4 days. Bars represent means, whiskers indicate standard deviation (N = 3 for each group).
Fig. 6 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 6. Lack of the aliphatic glucosinolate (GSL) Pren (107) in B. vulgaris and spiking of pure (intact) Pren for establishing the limit of detection. A. Total ion chromatogram for the three dominating peaks in G-type B. vulgaris (dGSL preparation). B. Extracted ion trace for desulfo Pren in the same extract as A, showing lack of detection. C, D, E. Results of serial spiking of the crude extract with serial 10-fold dilutions of Pren before the desulfation procedure, showing linearity also at low levels and ability to detect trace levels.
Fig. 9 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 9. Extracted ion HPLC-MS chromatograms of desulfoglucosinolates prepared from glucosinolates (GSLs) in seeds (A–F) or leaves (G) of Reseda luteola and seeds of Reseda odorata (H). The three major peaks (A, B, C) represent PE, IM and BAR, much like many Barbarea spp. Focus on minor peaks (D) allowed conclusive identification of EBAR, confirmed by tR and the characteristic MS2 spectrum. A range of putative derivatives were not detected (E, F), but an unidentified hydroxybutylGSL was present (G), as was a known glycoside in R. odorata.
Fig. 5 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 5. MS2 spectra of desulfated glucosinolates (GSLs) confirming the identity of two 2-3homoMet-derived GSLs in C. hirsuta. While the spectrum of desulfo Buen contains only the usual fragments for this type of dGSL (a, [anhydroGlc+Na]+; b, [thioGlc+Na]+), the spectrum of desulfo Peen contains an additional usual fragment (c, [M-anhydroGlc Na]+) and two unusual + fragment ions suggesting a structure-specific cyclization and exchange of O during fragmentation: 201, [gluconolactone Na]+ and 152, [C H NS Na]+. + 6 11 + The unusual fragments can be rationalized as fragment a plus O and fragment c minus O, respectively.
Fig. 3 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 3. HPLC-MS chromatograms of desulfoglucosinolates (dGSLs) prepared from glucosinolates (GSLs) in Planodes virginica (A) and Nasturtium officinale (B–C) seeds, showing qualitative similarities and quantitative contrasts. Major peaks (B) from N. officinale revealed many of the same GSLs as in A, but levels of EBAR (40R) were much lower while levels were much higher for the biosynthetic precursor PE (105). A focus on trace peaks from N. officinale (C) revealed sharp peaks representing a range of minor constituents. Due to the closely eluting peaks, the latter chromatograms (B–C) were made by combining extracted ion chromatograms corresponding to [M+Na]+ of the indicated dGSLs. In C, the m/z 366 signal of d105 was omitted to allow visualization of minor coeluting peaks. An asterisk after a peak number indicates tentative identification. HPLC-MS conditions as in Olsen et al. (2016). TIC, total ion chromatogram, EIC, extracted ion chromatogram.
Fig. 4 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 4. HPLC-MS chromatogram of desulfoglucosinolates (dGSLs) prepared from glucosinolates (GSLs) from leaves (A) and petioles (B) of horseradish (Armoracia rusticana), focusing on trace level GSLs. The chromatograms were made by combining extracted ion chromatograms corresponding to [M+Na]+ of the indicated dGSLs, from analyses that were much overloaded with respect to the dominating dGSL d107 from Pren. In panel A, an insert shows magnification of the chromatogram from 5.2 to 5.8 min. Neither suggested BAR nor EBAR were detectable. In panel B, only extracted ion chromatograms of m/z 382 (BAR/EBAR), 352 (BZ), 366 (PE), 380 (3PP), 394 (4PB), 408 (5PP at high tR and 6mSOh at 5.4 min), 422 (7mSOh) and 436 (8mSOo) are included. Unlabeled trace peaks did not exhibit a combination of tR and m/z suitable for any of the mentioned candidates. HPLC conditions as in Olsen et al. (2016). Panel A depicts analysis of the Copenhagen garden accession; panel B from the naturalized population at Lake Fures¨o. An asterisk after a peak number indicates tentative identification.
Fig. 2 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 2. Detection of thioglucose-acylated glucosinolates (GSLs) by HPLC-MS of desulfated derivatives prepared from the indicated species. (A–C) Analysis of seeds of Arabidopsis thaliana Col-0 used as reference material for characteristic GSLs. Shown are the total ion chromatogram (A) and extracted ion chromatograms for sodium adducts of desulfo 6′Bz 4BzOb (d125) (B) and desulfo 6′Bz 4mSb (d127) (C). (D–F) Analysis of seeds of Barbarea grayi for dominating GSLs. Shown are total ion chromatograms (D), and extracted ion chromatograms for sodium adducts of desulfo 6'iF BAR (d131S) (E) and desulfo 6'iF PE (d129) (F).
Fig. 1 in Glucosinolate profiles and phylogeny in Barbarea compared to other tribe Cardamineae (Brassicaceae) and Reseda (Resedaceae), based on a library of ion trap HPLC-MS/MS data of reference desulfoglucosinolates
Fig. 1. MS2 spectra of pairs of desulfoglucosinolates with and without a side chain double bond. Four short chain desulfoglucosinolates were investigated, including Na+ adducts of all (A–D) and in addition H+ adducts of the methylthio substituted (E–F), as indicated in each spectrum. The desulfo derivative of the putative 9mSn ([89]), poorly characterized in the literature, was also investigated (G).
Fig. 7 in Comparison of glucosinolate diversity in the crucifer tribe Cardamineae and the remaining order Brassicales highlights repetitive evolutionary loss and gain of biosynthetic steps
Fig. 7. Stages in the biosynthesis of parent glucosinolates (GSLs) without (A) or with (B) chain elongation of the precursor standard amino acid. A CYP79 enzyme catalyzes the first reaction in the known (cytosolic) core structure biosynthesis pathways, followed by six enzymatic steps constituting the remaining core structure biosynthesis pathway, abbreviated "r. csb". For GSLs without chain elongation (A), the CYP79 catalyzed reaction is the committed step. For GSLs needing chain elongation (B), however, the chain elongation machinery as well as transport ("T") across the chloroplast membrane and reversible amino transferase reactions collectively constitute the committed step, illustrated as a box-like reaction arrow containing the individual reactions.
Fig. 4 in Comparison of glucosinolate diversity in the crucifer tribe Cardamineae and the remaining order Brassicales highlights repetitive evolutionary loss and gain of biosynthetic steps
Fig. 4. All glucosinolates (GSLs) derived from aliphatic amino acids known from the tribe Cardamineae. The constant part of the GSLs is abbreviated GSL in most structures and exemplified in case of 107. Abbreviations of individual GSLs follow a comprehensive system explained in the text (Section 1.1.); spaces have occasionally been inserted in some long names and abbreviations for easier reading. BCAA; branched chain amino acid.
Fig. 1 in Comparison of glucosinolate diversity in the crucifer tribe Cardamineae and the remaining order Brassicales highlights repetitive evolutionary loss and gain of biosynthetic steps
Fig. 1. An archetypic glucosinolate-myrosinase system, leading to an isothiocyanate (A) and an oxazolidine-2-thione (B) from myrosinase-catalyzed hydrolysis of two ancient glucosinolates, 11 and 31. The hydrolysis reactions are unbalanced; water is an additional reactant and glucose, sulfate and hydrogen ion are also released during the myrosinase-catalyzed hydrolysis. A rearrangement precedes the formation of isothiocyanate (Blaˇzevi´c et al., 2020).
Fig. 3 in Comparison of glucosinolate diversity in the crucifer tribe Cardamineae and the remaining order Brassicales highlights repetitive evolutionary loss and gain of biosynthetic steps
Fig. 3. All glucosinolates (GSLs) derived from aromatic amino acids known from the tribe Cardamineae. The constant part of the GSLs is abbreviated GSL in most structures and exemplified in case of 11, a similar system is used for 6′-isoferuloylated GSLs as exemplified for 129. Abbreviations of individual GSLs follow a comprehensive system systematically explained in an accompanying paper (Agerbirk et al., 2021); spaces have occasionally been inserted in some long names and abbreviations for easier reading. The semisystematic name of "glucobarbarin" is (S)-2-hydroxy-2-phenylethylGSL, and for "epiglucobarbarin" it is (R)-2-hydroxy-2-phenylethylGSL.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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