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Figure 5 in Multiplex-PCR differentiation of two Hyalomma and two Haemaphysalis species (Acari: Ixodidae)

Figure 5. One percent agarose gel electrophoresis stained with Cyber Safe® showing ITS2 fragments amplified using primer pairs Fanas/Ran for Hyalomma anatolicum (amplicon size 749 bp) and Fanas/Ras for Hy. asiaticum (amplicon size 408 bp) (A), COI fragments amplified using primer pairs Fsul/Rpun and Fsul/Rsul for Haemaphysalis punctata (amplicon size 524 bp) and Ha. sulcata (amplicon size 614 bp) (B). (100 bp DNA ladder).

Figure 2 in Multiplex-PCR differentiation of two Hyalomma and two Haemaphysalis species (Acari: Ixodidae)

Figure 2. The ventral morphological aspect of representative male specimens of Haemaphysalis punctata (A) collected from Mazandaran province and Ha. sulcata (B) collected from Lorestan province; white arrows showing position of character spur of coxa IV (size of specimens was not considered).

Figure 1 in Multiplex-PCR differentiation of two Hyalomma and two Haemaphysalis species (Acari: Ixodidae)

Figure 1. The dorsal morphological aspect of representative male specimens of Hyalomma anatolicum (A) and Hy. asiaticum (B) both collected from Lorestan province; white and red arrows showing position of characters cervical grooves and dorsal posterior margin of the basis capituli, respectively (size of specimens was not considered).

Figure 4 in Multiplex-PCR differentiation of two Hyalomma and two Haemaphysalis species (Acari: Ixodidae)

Figure 4. Fourth female coxal spur of Haemaphysalis sulcata (A) and Ha. punctata (B), both collected from Mazandaran province, Iran.

Figure 3 in Multiplex-PCR differentiation of two Hyalomma and two Haemaphysalis species (Acari: Ixodidae)

Figure 3. General schema of representative female dorsal scutum of Hyalomma anatolicum (A) and Hy. asiaticum (B), both collected from Lorestan province (size of specimens was not considered).

Fig. 6 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 6 Phenotype associated with dynein, kinesin, isocitrate dehydrogenase and citrate synthase mRNA subjected to RNAi in female ticks via injection with the corresponding dsRNA. a Dynein dsRNA injection. b Kinesin dsRNA injection. c Isocitrate dehydrogenase dsRNA injection. d Citrate synthase dsRNA injection. e GFP dsRNA injection, control. f No injection, control. Scale-bars: 5 mm

Fig. 7 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 7 Digital micrographs of salivary gland acinar morphological changes in unfed female H. longicornis after RNAi.The time at which the tick bit the host and began sucking blood was recorded as day 0. a–e Dynein dsRNA injection. f–j Kinesin dsRNA injection. k–o Isocitrate dehydrogenase dsRNA injection. p–t Citrate synthase dsRNA injection. u–y GFP dsRNA injection, control. Scale-bars: 25 µm

Fig. 4 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 4 KEGG pathway enrichment analysis of the differentially expressed proteins in 5 different Clusters. Terms with a P-value <0.05 were used to draw the column diagrams. a–e KEGG pathway enrichment for the proteins in Cluster 1 to Cluster 5

Fig. 3 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 3 GO functional annotations for all the differentially expressed proteins. a–c GO annotations of differentially expressed proteins in the salivary glands of partially fed ticks compared with unfed ticks (115:114). d–f GO annotations of differentially expressed proteins in the salivary glands of mated semi-engorged ticks compared with partially fed ticks (116:115). g–i GO annotations of differentially expressed proteins in the salivary glands of engorged ticks compared with mated semi-engorged ticks (117:116). Abbreviations: BP, biological process; CC, cellular component; MF, molecular function; CO, cellular component organization or biogenesis

Fig. 5 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 5 RT-qPCR analyzed the mRNA expression levels of dynein, kinesin, isocitrate dehydrogenase, and citrate synthase during the four feeding stages of salivary gland development

Fig. 2 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 2 Statistics and cluster analysis for the identified proteins and their expression levels in the salivary glands of female H. longicornis. a Venn diagram showing the number of proteins (with CV <20%) identified in the three experiments. b Venn diagram showing the number of proteins with quantitative information. c Cluster analysis according to trends in protein expression in the salivary glands of female ticks

Fig. 1 in Salivary gland proteome analysis of developing adult female HaemaphYSaliS longiCorniS ticks: molecular motor and TCA cycle-related proteins play an important role throughout development

Fig. 1 Workflow for quantitative proteomics analysis of changes in protein expression in the salivary glands of female H. longicornis during the blood-feeding process

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Fig. 2 in Effects of introduced sika deer (Cervus nippon) and population control activity on the distribution of Haemaphysalis ticks in an island environment

Fig. 2. Seasonal changes in tick abundance of the dominant species (A) H. megaspinosa, (B) H. longicornis, and (C) H. cornigera on Niijima Island. (Broken line with black dot: Larvae, Dotted-dashes line with back triangles: Nymphs, Solid line with crosses: Adults).

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Fig. 3 in Effects of introduced sika deer (Cervus nippon) and population control activity on the distribution of Haemaphysalis ticks in an island environment

Fig. 3. Statistical summary of the abundances of H. megaspinosa larvae. (A) Days since the last sika deer was captured in August–November, (B) days since the last sika deer was captured in August–November for the high sika deer group, and (C) days since the last sika deer was captured in August–November for the low sika deer group. (X mark inside box: mean, lower and upper side of the box: first and third quartiles, line inside box: median, lower and upper error lines 10th and 90th percentiles, respectively, circles: data falling outside 10th and 90th percentiles).

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Fig. 1 in Effects of introduced sika deer (Cervus nippon) and population control activity on the distribution of Haemaphysalis ticks in an island environment

Fig. 1. Map of Niijima Island, with locations of foot snare traps (black circle), and tick collection routes for the tick survey conducted June, August, and November of 2018 and February of 2019 (orange area). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

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Fig. 2 in Where have all the grouse ticks gone? Apparent decline in collections of Haemaphysalis chordeilis Packard

Fig. 2. Published records of H. chordeilis (N = 96) by collection month. Records that did not specify collection month are excluded. Asterisks denote autumn months where hunting season is open for most game birds in North America, which likely influenced collections of H. chordeilis.

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Fig. 3 in Where have all the grouse ticks gone? Apparent decline in collections of Haemaphysalis chordeilis Packard

Fig. 3. Geographic locations of H. chordeilis records (N = 152). Records with no location information are excluded. In cases where a specific location (town or site) was not given, the state or county midpoint was used instead, taken from https://www. mapdevelopers.com/geocode_tool.php. Base map developed by the North American Commission for Environmental Cooperation (CEC) and downloaded from https://www.sciencebase.gov/catalog/item/ 4fb555ebe4b04cb937751db9. Species range maps developed by the U.S. Geological Survey Patuxent Wildlife Research Center based on National Breeding Bird Survey data from 2011 to 2015 and downloaded from https://www.mbr-pwrc.usgs.gov/bbs/shape_ ra15.html. Map created in QGIS Desktop v3.22.3.

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Fig. 1 in Where have all the grouse ticks gone? Apparent decline in collections of Haemaphysalis chordeilis Packard

Fig. 1. Published records of H. chordeilis (N = 161) by decade. Note that when no year was given for the collection, the year of the publication was used, so some records may have occurred earlier.

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Figure 2 in Molecular identification of Haemaphysalis sulcata (Acari: Ixodidae) larval stages collected using the Berlese funnel in Northern Iran

Figure 2. Phylogenetic tree generated based on ITS2 sequence data of the Haemaphysalis species of this study and sequences retrieved from GenBank database constructed using Bayesian Inference method. The main clade of tree separated by a yellow rectangular box. The taxa were defined with a name and GenBank accession number and taxa of the present study is bold. Posterior probability values inserted in the place of nodes. Branch lengths are proportional to the evolutionary changes.