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24,097 results for “Hemiptera”
Draft genome assembly version 1 of the meadow spittlebug Philaenus spumarius (Linnaeus, 1758) (Hemiptera, Aphrophoridae)
<p>We sequenced the genome of the meadow spittlebug, <em>Philaenus spumarius </em>(Linnaeus, 1758), the main insect vector of <em>Xylella fastidiosa </em>Wells et al. 1987 in Europe (Saponari et al., 2014), using 10x Chromium linked-reads. A single <em>P. spumarius</em> adult female from Portugal (Fontanelas, Sintra; GPS location: 38°50'15.75"N; 9°25'20.77"W), collected in September of 2018, was selected for genome sequencing. This population was initially surveyed for colour polymorphism in 1988 (Quartau & Borges, 1997) and was later included in phylogeographic and population genomic studies of this species (Rodrigues et al., 2014; Seabra et al., unpublished). It is also geographically close to the population from which the individual used for the first partial genome assembly was collected (Rodrigues et al., 2016). The availability of this previous genetic information contributed to the choice of this population as the source of genomic material for whole genome sequencing. A subset of males from the same collection date were analysed for genitalia morphology to confirm species identification, as the best diagnostic characters are the appendages of the aedeagus (Drosopoulos & Quartau, 2002).</p> <p>The genomic DNA of the <em>P. spumarius</em> adult from Sintra was extracted using Illustra Nucleon Phytopure kit according to the manufacturer’s instructions (GE Healthcare). We assessed the quality and concentration of the DNA using Femto fragment analyser (Agilent). 10x Chromium library preparation and Illumina genome sequencing (HiSeq X, 150bp paired-end) were performed by Novogene Bioinformatics Technology Co, Beijing, China, in accordance with standard protocols.</p> <p>To create the <em>de novo</em> 10x Chromium assembly we ran Supernova 2.1.1 (Weisenfeld et al., 2017) on the 10x Chromium linked-read data with default parameters, using 1.0 billion reads corresponding to 56X coverage. To improve the initial supernova assembly, we performed iterative scaffolding using all of the 10x raw data (2.3 billion of reads). We ran two rounds of Scaff10x (https://github.com/wtsi-hpag/Scaff10X), followed by mis-assembly detection and correction with Tigmint (Jackman et al., 2018). This was followed by a final round of scaffolding with ARCS (Yeo et al., 2018). The assembly was checked for contamination using the BlobTools pipeline (version 0.9.19; Laetsch and Blaxter 2017; Kumar et al., 2013) and k-mer content was analysed with the KAT comp tool (Mapleson et al., 2017). In order to perform these analyses, it was necessary to remove the 10x linked barcodes from the reads with the script process_10xReads.py (https://github.com/ucdavis-bioinformatics/proc10xG). We assessed the quality of our draft genome assembly by searching for conserved, single copy, arthropod genes (n=1,066) with Benchmarking Universal Single-Copy Orthologs (BUSCO) v3.0 (Waterhouse et al., 2018).</p> <p>With the above assembly procedure, we obtained a final assembly of 2.7 Gb, having a scaffold N50 length of 116 Kb (contig N50 = 18 Kb) and the longest scaffold was 3.7 Mb. The length of the assembly was consistent with the genome size estimated by flow cytometry (Rodrigues et al., 2016). The k-mer distribution indicated high heterozygosity, estimated at 2.3%. BlobTools analyses revealed the presence of contigs assigned to <em>Sodalis </em>spp. (Enterobacteriaceae), a symbiont in members of tribe Philaenini (Koga et al., 2013). These contigs were filtered from the final assembly. Gene completeness assessment shows that 956 (89.6%) among 1,066 BUSCOs were found as complete copies, with only 26 (2.4%) missing. Of the BUSCOs that were detected, 878 (82.4%) were complete and single-copy, 78 (7.3%) were complete and duplicated and 84 (7.9%) were fragmented.</p> <p>In conclusion, due in part to high (2.3%) heterozygosity levels, the <em>P. spumarius</em> version 1 genome assembly is highly fragmented. Nonetheless, the assembly is considered complete and is likely to contain the majority of the gene content of <em>P. spumarius.</em></p>
Genome sequence of the banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae) and its symbionts
<p><strong><em>Pentalonia nigronervosa</em> v1 frozen release</strong></p> <p>Genome assembly: Pentalonia_nigronervosa.v1.scaffolds.fa.gz</p> <p>BRAKER2 gene models: Pentalonia_nigronervosa.v1.scaffolds.gff</p> <p>BRAKER2 protein sequences: Pentalonia_nigronervosa.v1.scaffolds.gff.aa.fa</p> <p>BRAKER2 protein sequences (longest transcript per gene only): Pentalonia_nigronervosa.v1.scaffolds.gff.aa.LTPG.fa</p> <p>BRAKER2 coding sequences: Pentalonia_nigronervosa.v1.scaffolds.gff.cds.fa</p> <p>InterProScan functional annotation: Pentalonia_nigronervosa.v1.scaffolds.gff.aa.LTPG.interproscan.tsv</p> <p><em>Pentalonia nigronervosa</em> v1 mitochondrial genome: Pentalonia_nigronervosa.v1.mt_genome.fa</p> <p><em>Buchnera aphidicola</em> (BPn) scaffolds: Buchnera_aphidicola_BPn.scaffolds.fa</p> <p><em>Wolbachia</em> (WolPenNig) scaffolds: Wolbachia_WolPenNig.scaffolds.fa</p> <p><strong><em>Myzus cerasi </em>v1.2 frozen release</strong></p> <p>Genome assembly: Myzus_cerasi.v1.2.scaffolds.fa</p> <p>BRAKER2 gene models: Myzus_cerasi.v1.2.scaffolds.gff</p> <p>BRAKER2 protein sequences: Myzus_cerasi.v1.2.scaffolds.gff.aa.fa</p> <p>BRAKER2 protein sequences (longest transcript per gene only): Myzus_cerasi.v1.2.scaffolds.gff.aa.LTPG.fa</p> <p>BRAKER2 coding sequences: Myzus_cerasi.v1.2.scaffolds.gff.cds.fa</p> <p><strong>Aphid orthogroups and species tree</strong></p> <p>Proteomes included in the analysis: proteomes.tar.gz</p> <p>Orthogroups: Orthogroups.txt</p> <p>Gene counts per orthogroup, per species: Orthogroups.GeneCount.csv</p> <p>Single copy conserved orthogroups used for species tree: Orthogroups_for_concatenated_alignment.txt</p> <p>Species tree alignment: SpeciesTreeAlignment.fa</p> <p>Rooted species tree: SpeciesTree_rooted.nwk</p> <p><strong>Bash script to run k-mer based assembly deduplication pipeline</strong></p> <p>File: disco_filter_dups.v1.1.sh</p> <p>This script will parse a discovar de novo assembly and remove scaffolds likely to be haplotigs based on their k-mer content and a self alignment of the assembly (see manuscript for details).</p> <p>The input discovar assembly needs to have white space in scaffold IDs replaced with "_" before running. Illumina reads should be unzipped before running.</p> <p>Usage:</p> <pre><code class="language-bash">sh disco_filter_dups.sh <./path_to_assembly> <./path_to_r1> <./path_to_r2> <homozyzgous_lower_cov> <homozyzgous_upper_cov> <nucmer_id_cutoff> <nucmer_cov_cutoff> <assembly_output_prefix> <threads> <./working_dir></code></pre> <p> </p> <p> </p> <p> </p> <p> </p> <p> </p>
Vibrational signals produced by wing buzzing in Cacopsylla pyrisuga males (Hemiptera: Psyllidae)
<p>High-speed camera (video files) and laser vibrometer (audio files) recordings of Cacopsylla pyrisuga males producing vibrational signals - a dataset accompanying the publication</p> <p>Polajnar J., Kvinikadze E., Harley A.W., Malenovský I. (2024) Wing buzzing as a mechanism for generating vibrational signals in psyllids (Hemiptera: Psylloidea). Insect Science. See the publication for details about the methodology used.</p> <p>The dataset additionaly includes tracked points at wing and abdomen tips from two videos, and an R script with instructions to read this data.</p>
Hemiptera-Phytoplasma-Plant dataset
<p>This is a database of Hemiptera-Phytoplasma-Plant (HPP) biological interactions worldwide. The database contains 1860 records of plant-phytoplasma pairwise associations, and 968 records of insect-phytoplasma pairwise associations. Only the earliest reported record of each specific association between a 16Sr phytoplasma subgroup and a host (plant or insect vector) is reported for each country in the database. Last update: Nov 2018</p> <p> </p>
Spreadsheet Template for Body Size Data for North American Hemiptera
<p>Body size data for North American Hemiptera extracted from The Insects and Arachnids of Canada:</p> <p>Hamilton, K.G.A., 1982. The insects and arachnids of Canada, Part 10. The Spittlebugs of Canada. Homoptera: Cercpidae. Research Branch Agriculture Canada Publication 1740:1-102.</p> <p>Kelton, L.A., 1978. The insects and arachnids of Canada, Part 4. The Anthocoridae of Canada and Alaska: Heteroptera, Anthocoridae. Research Branch Agriculture Canada Publication 1639:1-101.</p> <p>Kelton, L.A., 1980. The insects and arachnids of Canada, Part 8. The plant bugs of the prairie provinces of Canada (Heteroptera: Miridae). Research Branch Agriculture Canada Publication 1703:1-408.</p> <p>Matsuda, R., 1977. The insects and arachnids of Canada, Part 3. The Aradidae of Canada: Hemiptera: Aradidae. Research Branch Agriculture Canada Publication 1634:1-116.</p>
Figure 4 in Éthologie sonore et statut acoustique de quelques Cigales thaïlandaises, incluant la description de deux espèces nouvelles (Hemiptera : Auchenorhyncha*, Cicadoidea, Cicadidae)
Figure 4 Pomponia linearis (Walker, 1850). Mâle (en haut) et femelle allotype (en dessous). (Photographies Michel Boulard)
Figures 13 in Éthologie sonore et statut acoustique de quelques Cigales thaïlandaises, incluant la description de deux espèces nouvelles (Hemiptera : Auchenorhyncha*, Cicadoidea, Cicadidae)
Figures 13 à 15 Terpnosia nonusaprilis n. sp. – 13 à 15, Holotype ♂, vues dorsale (13) et sous abdominale (14), fémur antérieur (15). (Dessins Hélène LeRuyet-Tan)
Figures 10 et 11 Tanna ventriroseus n in Éthologie sonore et statut acoustique de quelques Cigales thaïlandaises, incluant la description de deux espèces nouvelles (Hemiptera : Auchenorhyncha*, Cicadoidea, Cicadidae)
Figures 10 et 11 Tanna ventriroseus n. sp. – 10 et 11, Conformation des pièces génitales d'un paratype ♂. (Dessins Hélène LeRuyet-Tan)
Figure 12 Terpnosia abdullah Distant, 1904 in Éthologie sonore et statut acoustique de quelques Cigales thaïlandaises, incluant la description de deux espèces nouvelles (Hemiptera : Auchenorhyncha*, Cicadoidea, Cicadidae)
Figure 12 Terpnosia abdullah Distant, 1904. Mise en évidence de la maculature latérotergale de l'abdomen. (Photographie Michel Boulard)
Figures 5-6 in Éthologie sonore et statut acoustique de quelques Cigales thaïlandaises, incluant la description de deux espèces nouvelles (Hemiptera : Auchenorhyncha*, Cicadoidea, Cicadidae)
Figures 5-6 Pomponia linearis (Walker, 1850). Conformation des pièces génitales mâles vues de profil, puis de l'arrière. (Dessins Hélène LeRuyet-Tan)
Figures 2-3 in Éthologie sonore et statut acoustique de quelques Cigales thaïlandaises, incluant la description de deux espèces nouvelles (Hemiptera : Auchenorhyncha*, Cicadoidea, Cicadidae)
Figures 2-3 Macrosemia tonkiniana (Jacobi, 1905), habitus typique du mâle (2) mise en évidence de la forme des opercules (3).
Figs. 1-2. 1 in Two new species of the genus Chloropepla (Hemiptera: Pentatomidae: Pentatominae) from Brazil*)
Figs. 1-2. 1 – Chloropepla paveli sp. nov. (male holotype). 2 – C. stysi sp. nov. (male holotype). Scale bars = 2 mm.
Figs. 15-17. Female genitalia. 15 in Two new species of the genus Chloropepla (Hemiptera: Pentatomidae: Pentatominae) from Brazil*)
Figs. 15-17. Female genitalia. 15 – Chloropepla paveli sp. nov., genital plates. 16-17 – C. stysi sp. nov., female genitalia. 16 – genital plates; 17 – gonocoxites and gonapophyses of ninth segment and ectodermal genital ducts. Scale bars = 1 mm (aac – anterior annular crest; cs – capsula seminalis; ch – chitinellipsen; g9 – gonapophyses 9; gc8 – gonocoxites 8; gc9 – gonocoxites 9; la8 – laterotergites 8; la9 – laterotergites 9; pac – posterior annular crest; pco – pars communis; pi – pars intermedialis; tvi – thickening of vaginal intima; X – segment X).
Figs. 7-14. Male external and internal genitalia. 7-10 in Two new species of the genus Chloropepla (Hemiptera: Pentatomidae: Pentatominae) from Brazil*)
Figs. 7-14. Male external and internal genitalia. 7-10 – Chloropepla paveli sp. nov. 7 – right paramere, dorsal view; 8 – phallus, dorsal view; 9 – phallus, ventral view; 10 – phallus, lateral view. Scale bars = 0.5 mm. 11-14 – C. stysi sp. nov. 11 – right paramere, dorsal view; 12 – phallus, dorsal view; 13 – phallus, ventral view; 14 – phallus, lateral view. Scale bars = 1 mm (aa – articulatory apparatus; cj – conjuntiva; dsd – ductus seminis distalis; dsp – ductus seminis proximalis; pht – phallotheca; ppht I – processus phallothecae I; ppht II – processus phallothecae II; ve – vesica; vp – vesica collar process).
Figs. 3-6. Male external genitalia. 3-4 in Two new species of the genus Chloropepla (Hemiptera: Pentatomidae: Pentatominae) from Brazil*)
Figs. 3-6. Male external genitalia. 3-4 – Chloropepla paveli sp. nov., pygophore. 3 – dorsal view; 4 – ventral view. 5-6 – C. stysi sp. nov., pygophore. 5 – dorsal view; 6 – ventral view. Scale bars = 1 mm (dr – dorsal rim; hy – hypandrium; mp – marginal process of dorsal rim; pa – paramere; vr – ventral rim; X – segment X).
Fig. 2 in Rediscovering Anhanga: redescription and considerations on its taxonomic placement (Hemiptera: Heteroptera: Pentatomidae)
Fig. 2. Lectotype of Anhanga modesta Distant, 1887.A – dorsal view; B – ventral view; C – lateral view; D – genital plates in postero-ventral view; E – labels. Scale not provided.
Fig. 4 in Rediscovering Anhanga: redescription and considerations on its taxonomic placement (Hemiptera: Heteroptera: Pentatomidae)
Fig. 4. Internal female genitalia of Anhanga modesta Distant, 1887. Abbreviations: aaf – anterior annular flange; cs – capsula seminalis; dr – ductusreceptaculi; g9 – gonapophyses IX; gc9 – gonocoxites IX; la8 – laterotergites VIII; la9 – laterotergites IX; or – orificium receptaculi; paf – posterior annular flange; pc – pars communis; pcs – finger-like process of capsula seminalis; pi – pars intermedialis; rs – ring sclerites; tvi – thickening of vaginal intima; va – vesicular area; X – tenth segment). Scale bar: 1 mm.
Fig. 1. Anhanga modesta Distant, 1887. A in Rediscovering Anhanga: redescription and considerations on its taxonomic placement (Hemiptera: Heteroptera: Pentatomidae)
Fig. 1. Anhanga modesta Distant, 1887. A – dorsal view; B – magnification of the head in ventral view, dashed line highlighting the basal segment of labium arising anteriorly to the imaginary line traversing head at anterior margin of eyes; C – ventral view; D – magnification of external scent efferent system of metathoracic scent glands; E – magnification of abdomen, highlighting the placement of the pair of trichobothria (arrows) along the imaginary line of spiracles on urosternites VI and VII. Scale bar: 5 mm.
Figs. 12-17. Pygophores, dorsal view. 12, N in New Species of Neotibilis Grazia & Barcellos (Hemiptera: Pentatomidae: Pentatomini)
Figs. 12-17. Pygophores, dorsal view. 12, N. (N.) parva; 13, N. (N.) chiapensis; 14, N. (N.) panamensis; 15, N. (N.) biguttata; 16, N. (N.) costaricensis; 17, N. (N.) fulvicornis. (from Grazia & Barcellos, 1994). Scale 1mm.
Figs. 7-11. N in New Species of Neotibilis Grazia & Barcellos (Hemiptera: Pentatomidae: Pentatomini)
Figs. 7-11. N. (N.) claviformis sp. nov. 7-8 parameres. 7, dorsal view of right paramere; 8, latero-external view of right paramere. 9-11 Phallus. 9, lateral view; 10, ventral view; 11, dorsal view (sg = secundary gonopore; ph = phalloteca; prv 1 = processus vesicae 1; prv 2 = processus vesicae 2).
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.