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207 results for “Huntingtin”
Dataset for the Huntingtin antibody screening study
<p><strong><span>This antibody characterization dataset is related to the F1000 research article openly available at F1000Research.</span></strong></p> <p><em>This dataset contains underlying data from a study that evaluated twenty commercial antibodies agaisnt Huntingtin in western blot, immunoprecipitation and immunofluorescence. The study is accessible on our Zenodo community (<a href="https://doi.org/10.5281/zenodo.11582780">https://doi.org/10.5281/zenodo.11582780</a>) and serves as a research tool to facilitate reproducible and reliable Huntingtin resarch.</em></p> <p><em>The Dataset is in the format of a zip file. Once downloaded, please expand the zip file to access the folders containing the underlying data for Western blot (Wb), immunoprecipitation (IP) and immunofluorescence (IF). </em></p>
Huntingtin structure is orchestrated by HAP40 and shows a polyglutamine expansion-specific interaction with exon 1
<p>Supplementary Data files to accompany manuscript by Harding et al "Huntingtin structure is orchestrated by HAP40 and shows a polyglutamine expansion-specific interaction with exon 1"</p> <ul> <li>Supplementary Data 1 - Multiple sequence alignment for HTT used for Consurf analysis</li> <li>Supplementary Data 2 - Multiple sequence alignment for HAP40 used for Consurf analysis</li> <li>Supplementary Data 3 - Apo HTT cryo-EM map </li> <li>Supplementary Data 4 - HTT-HAP40 Q23 regularised SAXS profile</li> <li>Supplementary Data 5 - HTT-HAP40 Q54 regularised SAXS profile</li> <li>Supplementary Data 6 - HTT-HAP40 Δexon 1 regularised SAXS profile</li> <li>Supplementary Data 7 - XL-MS data</li> <li>Supplementary Data 8 - HTT-HAP40 ensemble weightings</li> <li>Supplementary Data 9 - HTT-HAP40 Q23 ensemble models</li> <li>Supplementary Data 10 - HTT-HAP40 Q54 ensemble models</li> <li>Supplementary Data 11 - HTT-HAP40 Δexon 1 ensemble models</li> </ul>
Data From: Global huntingtin knockout in adult mice leads to fatal neurodegeneration that spares the pancreas
Open the record for dataset details and reuse information.
High resolution cryoEM structure of huntingtin in complex with HAP40
<p><strong>High resolution cryoEM structure of huntingtin in complex with HAP40</strong></p> <p>This dataset relates the following depositions in the PDB: 6X9O and EMDB: EMD-22106 which are the structure solution of HTT-HAP40 complex at 2.6 angstrom resolution by cryoEM. </p> <p>Files contained within this dataset are detailed in the "Upload_information.xlsx" file. </p> <p> </p>
Investigating huntingtin DNA binding – plasmid EMSA with full-length HTT Q23/Q46
<p>Huntingtin structure-function open lab notebook.</p> <p> </p> <p>NB: plasmid concentration should read 0.5 mg/mL not 0.5 mg/uL.</p>
ROS-specific Huntingtin Interactions: Crosslinking Optimization
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions.</p>
ROS-specific Huntingtin Interactions: Fractionation Optimization
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions.</p>
ROS-specific Huntingtin Interactions: Oxidative Stress Optimization H2O2
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions.</p>
ROS-Specific Huntingtin Interactions: IP Optimization in Patient-Derived Cells
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions.</p>
ROS-specific Huntingtin Interactions: GFP reactivation assay WT vs HD mouse striatal cells second attempt
<p>Optimization step in the lead up to phenotypic analysis of ROS-dependent huntingtin interacting proteins.</p>
ROS-specific Huntingtin Interactions: GFP reactivation assay WT vs HD mouse striatal cells
<p>Optimization step in the lead up to phenotypic analysis of ROS-dependent huntingtin interacting proteins.</p>
ROS-specific Huntingtin Interactions: GFP reactivation assay optimization in HEK 293 cells
<p>Optimization step in the lead up to phenotypic analysis of ROS-dependent huntingtin interacting proteins.</p>
ROS-specific Huntingtin Interactions: Testing inducible expression of huntingtin-specific chromobodies
<p>Testing step in the generation of an inducible system for expressing YFP-tagged huntingtin-specific intrabodies in live cells.</p>
ROS-specific Huntingtin Interactions: Cloning huntingtin-specific chromobodies into pTRE-3G
<p>Cloning step in the generation of an inducible system for expressing YFP-tagged huntingtin-specific intrabodies in live cells.</p>
ROS-specific Huntingtin Interactions: G418 kill curve in TruHD fibroblasts
<p>Optimization step in the development of an inducible system expressing YFP-tagged huntingtin-specific intrabodies for stable transfection in TruHD fibroblasts.</p>
ROS-specific Huntingtin Interactions: Comparing transfection methods for inducible expression of huntingtin-specific chromobody
<p>Optimization step in the development of an inducible system expressing YFP-tagged huntingtin-specific intrabodies for stable transfection in TruHD fibroblasts.</p>
ROS-specific Huntingtin Interactions: Inducible huntingtin-specific chromobody expression by nucleofection
<p>Optimization step in the development of an inducible system expressing YFP-tagged huntingtin-specific intrabodies for stable transfection in TruHD fibroblasts.</p>
ROS-Specific Huntingtin Interactions: ROS Source Optimization in Mouse Striatal Cells
<p>Optimization step in the lead up to mass spec identification of ROS-specific huntingtin protein-protein interactions. </p>
ROS-specific Huntingtin Interactions: GFP reactivation assay in HD patient fibroblasts
<p>Optimization step in the lead up to phenotypic analysis of ROS-dependent huntingtin interacting proteins. </p>
Huntingtin intrabody and nanobody literature review 10th January 2018
<p>Huntingtin structure-function open lab notebook project. </p> <p>To kick off the new year, I have been doing some reading on huntingtin intrabodies and nanobodies and have written this up into an informal literature review. These are small Ig domain proteins which specifically bind different regions of huntingtin. All of those which I have written about, bind the exon 1 of huntingtin, some with specificity for the N17 region, whereas others bind the polyproline or proline rich domains.</p> <p>Thanks to Prof Ray Truant, who kindly provided me with some nanobody clones, I have already expressed and purified iVHH4. I plan to clone, express and purify Happ1 and VL12.3, both of which are single Ig domain proteins, and along with iVHH4, test binding to my huntingtin protein samples and see if they help stabilize my samples.</p> <p>http://labscribbles.com/2018/01/10/huntingtin-intrabody-and-nanobody-literature-review/ </p>
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.