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8 results for “IC50”
Determining the IC50 values of 30 legacy ACVR1/ALK2 inhibitors
<p>A large number of ACVR1/ALK2 inhibitors were previously synthesised by Paul Brenner’s team (Target Discovery Center, University of Oxford) for the purpose of treating Fibrodysplasia Ossificans Progressiva (FOP). Although these compounds were not designed with blood-brain-barrier permeability in mind, they can serve as good bench-marks for my cellular assays. Therefore, 30 of these legacy compounds were chosen to be tested before other new bespoke Diffused Intrinsic Pontine Glioma (DIPG) compounds from Ontario Institute for Cancer Research (OICR) and Charles River Laboratories (CRL).</p>
Determining the IC50 of M4K1062 with ACVR1/ALK2-NanoLuc and different concentrations of Tracer-6908
<p>The EC50 of Tracer-6908 with ACVR1-c-nanoLuc and ideal conditions for the target engagement assay have been determined in the previous post. However, it is still necessary to verify that the IC50 values determined in the assay are closed enough approximation to the actual IC50 values of the compounds. If the IC50 values are strongly influenced by the concentration of Tracer-6908 used, multiple assays with progressively lower Tracer-6908 will need to be performed. The actual IC50 of the compounds can then be estimated by plotting the Tracer-6908 concentrations against the corresponding IC50 values and regressing to no Tracer-6908. To address this, multiple nanoBRET target engagement assays were setup with increasing concentrations of Tracer-6908 with the same serial dilution of M4K1962 (starting from the EC50 of 65nM).</p>
Determining the dual luciferase ALK5 IC50 values of 30 legacy ACVR1/ALK2 inhibitors
<p>A large number of ACVR1/ALK2 inhibitors were previously synthesised by Paul Brenner’s team (Target Discovery Center, University of Oxford) for the purpose of treating Fibrodysplasia Ossificans Progressiva (FOP). Although these compounds were not designed with blood-brain-barrier permeability in mind, they can serve as good bench-marks for my cellular assays. Therefore, 30 of these legacy compounds were chosen to be tested before other new bespoke Diffused Intrinsic Pontine Glioma (DIPG) compounds from Ontario Institute for Cancer Research (OICR) and Charles River Laboratories (CRL). Inhibition of TGFBR1/ALK5 leads to cardiac toxicity. Since I have not yet been able to establish a robust nanoBRET target engagement assay for TGFBR1/ALK5, I will have to resort to dual luciferase promoter assay (orthologous assay) for the time being.</p> <p>For my corresponding opennotebook post, please visit the following page:</p> <p>https://openlabnotebooks.org/determining-the-dual-luciferase-alk5-ic50-values-of-30-legacy-acvr1-alk2-inhibitors/</p>
supplementary files IC50
<p>supplementary files of PM2.5 mediates mouse testis Sertoli TM4 cell injury by reducing cellular NAD<sup>+</sup></p> <p>IC50 of PM2.5</p>
Figures of IC50 of each compound by graph pad prisim
<p>This includes IC50 data results for each compound by using graph pad prisim as a pzfx format and JPG format</p>
Determining the nanoBRET AL2K IC50 values of 24 new ACVR1/ALK2 inhibitors
<p>In an effort to develop clinical compounds for Diffused Intrinsic Pontine Glioma (DIPG) treatment, new analogues of ACVR1/ALK2 inhibitors are continuously synthesised by Ontario Institute for Cancer Research (OICR) and Charles River Laboratories (CRL). I will provide prompt feedback of the cellular assay results to guide their design of new compounds.</p> <p>The blog post for this page can be found on the SGC opennotebook site:</p> <p>https://openlabnotebooks.org/determining-the-nanobret-al2k-ic50-values-of-24-new-acvr1-alk2-inhibitors/</p>
IC50 PDF Figures
<p>This file includes figures of IC50 as a PDF format</p>
Inhibition of a G9a/DNMT network triggers an immune-mediated bladder cancer regression treated with CM272 (IC50)
GEO Series GSE115544. Homo sapiens. 12 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.