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19 results for “Identification success”
Peatland restoration in Norway – evaluation of ongoing monitoring and identification of plant indicators of restoration success
<p>Norway launched a national action plan on wetland restoration in 2016. So far, 90% of the restoration effort has been on peatland restoration, with about 140 mires restored so far. There are three main restoration goals stated in the action plan: 1) Limit greenhouse gas (GHG) emissions, 2) climate adaptation, and 3) improve ecological condition. Quantifying the outcome of the restoration actions is necessary to evaluate whether the goals of the action plan are met. A vegetation monitoring protocol was suggested before restoration started and has been implemented at five restoration sites. As the peatland restoration effort in Norway is increasing, it is timely to evaluate if the data currently collected can measure peatland restoration outcomes. We evaluate the monitoring protocol based on statistical analyses of the data collected at two sites, describe how indicator species can be identified using generalized composition data used as the basis for classifying habitats in Norway (EcoSyst framework), and suggest the way forward for peatland restoration monitoring in Norway. Data collected according to the monitoring protocol can document changes in species composition at restoration sites but has limitations when the ecological complexity at the sites increases and reference sites are unavailable. We argue that adjusting the monitoring protocol will: 1) Facilitate alignment with existing peatland research; 2) connect better with monitoring programs where data is collected applying EcoSyst framework principles; and 3) enable upscaling to cover the wide variation emerging in peatland restoration.</p>
Peatland restoration in Norway – evaluation of ongoing monitoring and identification of plant indicators of restoration success
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Identification of quantitative trait loci and associated candidate genes for pregnancy success in Angus – Brahman crossbred heifers
<p>Development of genomic tools to identify females with high genetic merit for reproductive function could increase the profitability and sustainability of beef production. Here, genome-wide association studies (GWAS) were performed on pregnancy outcome traits from a population of Angus – Brahman crossbred heifers. Furthermore, a validation GWAS was performed using data from another location. Heifers were genotyped with the Bovine GGP F250 array that contains ~250,000 SNPs. In the discovery population, heifers were bred in winter breeding seasons involving a single round of timed artificial insemination (AI) followed by natural mating for three months. Three phenotypes were analyzed: pregnancy outcome to first-service AI (PAI; n = 1481), pregnancy status at the end of the breeding season (PEBS; n = 1725), and pregnancy score (Pregscore where 1 = pregnant to first-service AI, 2 = pregnant to bull, 3 = not pregnant; n =1481). The heritability for PAI was estimated as 0.149. One large quantitative trait locus (QTL) that explained ~3% of the genetic variation for PAI was found on BTA7, in a region containing a cluster of γ-protocadherin genes and SLC25A2. Other QTLs explaining between 0.5-1% of the genetic variation were found on BTA12 and 25. The heritability of PEBS was estimated at 0.122. A large QTL on BTA7 was synonymous with the QTL for PAI, with minor QTL located on BTA5, 9, 10, 11, 19, and 20. Estimated heritability for Pregscore was 0.189. There was a large QTL on BTA7 synonymous with the other two traits as well as smaller QTLs on BTA1, 10, 15, 18, 19, and 20. The validation population for pregnancy status at the end of the breeding season were Angus-Brahman crossbred heifers bred by natural mating. In concordance with the discovery population, the large QTL on BTA7 and QTL on BTA10, 12 and 18 were identified. In summary, QTL and candidate SNPs associated with pregnancy outcomes in beef heifers were identified, including a large QTL associated with a group of protocadherin genes. Confirmation of these associations with larger populations could lead to the development of genomic estimates of reproductive function in beef cattle.</p>
Identification of quantitative trait loci and associated candidate genes for pregnancy success in Angus – Brahman crossbred heifers
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Data from: Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe (Gallinago media)
We assembled the great snipe blood transcriptome using data from fourteen lekking males, in order to de novo identify candidate genes related to sexual selection, and determined the expression profiles in relation to mating success. The three most highly transcribed genes were encoding different haemoglobin subunits. All tended to be overexpressed in males with high mating success. We also called Single Nucleotide Polymorphisms (SNPs) from the transcriptome data and found considerable genetic variation for many genes expressed during lekking. Among these we identified 14 polymorphic candidate SNPs that had a significant genotypic association with mating success (number of females mated with) and/or mating status (mated or not). Four of the candidate SNPs were found in HBAA (encoding the haemoglobin α-chain). Heterozygotes for one of these and one SNP in the gene PABPC1 appeared to enjoy higher mating success compared to males homozygous for either of the alleles. In a larger dataset of individuals we genotyped 38 of the identified SNPs but found low support for consistent selection since only one of the zygosities of previously identified candidate SNPs and none of their genotypes were associated with mating status. However, candidate SNPs generally showed lower levels of spatial genetic structure compared to non-candidate markers. We also scored the prevalence of avian malaria in a sub-sample of birds. Males infected with avian malaria parasites had lower mating success in the year of sampling than non-infected males. Parasite infection and its interaction with specific genes may thus affect performance on the lek.
Data from: Successful carnivore identification with faecal DNA across a fragmented Amazonian landscape
The use of scat surveys to obtain DNA has been well documented in temperate areas, where DNA preservation may be more effective than in tropical forests. Samples obtained in the tropics are often exposed to high humidity, warm temperatures, frequent rain, and intense sunlight, all of which can rapidly degrade DNA. Despite these potential problems, we demonstrate successful DNA amplification and sequencing for faeces of carnivores collected in tropical conditions and quantify how sample condition and environmental variables influence the success of PCR amplification and species identification. Additionally the feasibility of genotyping nuclear microsatellites from jaguar (Panthera onca) faeces was investigated. From October 2007 to December 2008, 93 faecal samples were collected in the southern Brazilian Amazon. A total of eight carnivore species was successfully identified from 71% of all samples obtained. Information theoretic analysis revealed that the number of PCR attempts before a successful sequence was an important negative predictor across all three responses (success of species identification, success of species identification from the first sequence and PCR amplification success), whereas the relative importance of the other three predictors (sample condition, season, and distance from forest) varied between the three responses. Nuclear microsatellite amplification of DNA from jaguar faeces had lower success rates (15–44%) compared with those of the mtDNA marker. Our results show that DNA identification of carnivore species from faecal samples works efficiently in the Amazon forest and can provide data on species occurrence as well as a valuable tool for genetic, ecological and conservation studies.
Figure 2 in Successful identification of the final instar nymph of Quintilia carinata (Thunberg) (Hemiptera: Cicadidae) by DNA extraction from the exuvium
Figure 2. Legs of the final instar nymph of Quintilia carinata: (A) Lateral view of left foreleg. acf, accessory tooth of femur; apt, apical tooth of tibia; bt, blade of tibia; f, femur; fc, femoral comb; itf, intermediate tooth of femur; pbt, point of blade of tibia; ptf, posterior tooth of femur; t, trochanter; ti, tibia; ta, tarsus. (B) Distal spines of mesotibia. (C) Distal spines of metatibia. Scale bars: (A) 2 mm, (B) 1 mm, (C) 1 mm.
Figure 1 in Successful identification of the final instar nymph of Quintilia carinata (Thunberg) (Hemiptera: Cicadidae) by DNA extraction from the exuvium
Figure 1. Exuvium of the final instar nymph of Quintilia carinata. (A) Lateral view. (B) Dorsal view of head. Scale bars: (A) 8 mm, (B) 2 mm.
Does Ultrasound Increase the First-pass Success of Epidural Space Identification in Obese Parturients
ClinicalTrials.gov study NCT04352283. IPD Sharing: NO. Countries: 1. Publications: 1.
Pretreatment Identification of Duloxetine Success in Neuropathic Pain Patients
ClinicalTrials.gov study NCT01363284. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Identification of Phenotypic Factors That Predict Success for Weight Loss and Long-term Weight Maintenance
ClinicalTrials.gov study NCT02043457. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Data from: Successful carnivore identification with faecal DNA across a fragmented Amazonian landscape
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Data from: Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe (Gallinago media)
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IDENTIFICATION OF GIFTED CHILDREN (ANALYSIS OF SPECIAL SUCCESSES AND ACHIEVEMENTS, DIAGNOSTICS OF POTENTIAL OPPORTUNITIES OF STUDENTS).
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Figure 3 in Successful identification of the final instar nymph of Quintilia carinata (Thunberg) (Hemiptera: Cicadidae) by DNA extraction from the exuvium
Figure 3. Ventral view of terminal segments of abdomen of female nymph.
Mitral Valve Reconstruction in Chronic Heart Failure (CHF): Identification of Predictors for a Successful Therapy
ClinicalTrials.gov study NCT00348829. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Identification of Predictors of Success for Lifestyle Modifications in Overweight Pre-diabetic Subjects
ClinicalTrials.gov study NCT00969007. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Identification of Criteria of the Success of the Endarterectomy in Chronic Pulmonary Post Embolic Hypertension
ClinicalTrials.gov study NCT00657722. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Identification and successful negotiation of a metabolic checkpoint in direct neuronal reprogramming
GEO Series GSE70921. Mus musculus. 8 samples. Type: Expression profiling by array.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.