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339 results for “In vitro models”
Data for: 3D in vitro modeling of the exocrine pancreatic unit using tomographic volumetric bioprinting
<p><strong>Abstract</strong></p> <div> <div> <p><span><span>Pancreatic ductal adenocarcinoma (PDAC) is the most frequent type of pancreatic cancer, one of the leading causes of cancer-related deaths worldwide. The first lesions associated with PDAC occur within the functional units of exocrine pancreas</span><span>. T</span><span>he crosstalk between PDAC cells and stromal cells plays a key role in tumor progression.</span><span> Thus,</span> <span>i</span></span><span><span>n vitro</span></span><span><span>, fully human models of the pancreatic cancer microenvironment are needed to foster the development of new, more effective therapies</span><span>.</span> <span>However,</span><span> it is challenging to make these models anatomically and functionally relevant. Here, we used tomographic volumetric bioprinting, a novel method to fabricate </span><span>three-dimensional </span><span>cell-laden constructs</span><span>,</span><span> to produce a </span><span>portion</span><span> of the </span><span>complex convoluted </span><span>exocrine pancreas</span> </span><span><span>in vitro</span></span><span><span>.</span><span> Human fibroblast-laden gelatin methacrylate-based pancreatic models were processed to reassemble the </span><span>tubuloacinar</span><span> structures of the exocrine pancreas and, then human pancreatic ductal epithelial (HPDE) cells overexpressing the KRAS oncogene (HPDE-KRAS) were seeded in the acinar lumen to reproduce the pathological exocrine pancreatic tissue. The growth and organization of HPDE cells within the structure was evaluated and the formation of a thin epithelium which covered the acini inner surfaces in a physiological way inside the 3D model was</span> <span>successfully</span> <span>demonstrated</span><span>. Interestingly, immunofluorescence assays revealed a significantly higher expressions of alpha smooth muscle </span><span>actin</span><span> (α-SMA) vs. </span><span>actin</span><span> in the fibroblasts co-cultured with cancerous than with wild-type HPDE cells. Moreover, α-SMA expression increased with time, and it was found to be higher in fibroblasts that laid closer to HPDE cells than in those </span><span>laying </span><span>deeper into the model. Increased levels of interleukin (IL)-6 were also quantified in supernatants from co-cultures of stromal and HPDE-KRAS cells. These findings correlate with inflamed tumor-associated fibroblast behavior, thus being relevant biomarkers to </span><span>monitor</span><span> the early progression of the disease and to target drug efficacy. </span></span><span> </span></p> </div> <div> <p><span><span>To our knowledge, this is the first</span> <span>demonstration of a </span><span>3D </span><span>bioprinted</span> <span>portion</span><span> of </span><span>pancreas that</span> <span>rec</span><span>apit</span><span>ulates</span> <span>its</span> <span>true 3-dimensional </span><span>microanatomy</span><span>,</span><span> and which shows </span><span>tumor triggered </span><span>inflammation</span><span>. </span></span><span> </span></p> </div> </div> <p> </p> <p><strong>Contents</strong></p> <p>This repository contains the raw data, materials list, protocols, and code necessary to reproduce the work in the namesake preprint.</p> <p> </p>
An inflamed human alveolar model for testing the efficiency of anti-inflammatory drugs in vitro
<p>The data set accompanies the study where we developed an inflamed human alveolar epithelium model and to test the resolution<strong><em> </em></strong>lipopolysaccharide (LPS)-induced inflammation <em>in vitro</em> with a corticosteroid, methylprednisolone (MP). A specific focus of the study was in macrophage phenotype shifts in response to these stimuli.</p> <p>The data set includes:</p> <p>- Pro-inflammatory marker (interleukin (IL)-8, tumor necrosis factor α (TNFα), IL1β) secretion data, analysed via ELISA, and cell viability (analysed via lactate dehydrogenase assay) of both monocultures (human monocyte-derived macrophages) and of the multicellular human alveolar model, composed of macrophages, dendritic cells, and epithelial cells. </p> <p>- Barrier permeability data of the multicellular model, assessed via labeled-dextran permeability assay. </p> <p>All the above-stated data is joined in the file: DraslerB_Frontiers 2020_Inflammatory model. Sample codes are explained int he first tabs. </p> <p>- Pro-inflammatory marker gene expression data of the multicellular model, assessed via real time RT-qPCR. The data prepared for analysis and analysed is joined to the above-mentioned folder, whereas the direct PCR runs are joined in the file: DraslerB_Frontiers 2020_Inflammatory model_PCR runs. </p> <p>- Confocal laser scanning microscopy raw files (lsm) of the multicellular model can be opened with an open source software Fiji, based on ImageJ. </p>
Early deficits in an in vitro striatal microcircuit model carrying the Parkinson's GBA-N370S mutation
<p><strong>ABSTRACT</strong></p> <p>Understanding medium spiny neuron (MSN) physiology is essential to understand motor impairments in Parkinson’s disease (PD) given the architecture of the basal ganglia. Here, we developed a custom three-chambered microfluidic platform and established a cortico-striato-nigral microcircuit partially recapitulating the striatal presynaptic landscape in vitro using induced pluripotent stem cell (iPSC)-derived neurons. We found that, cortical glutamatergic projections facilitated MSN synaptic activity, and dopaminergic transmission enhanced maturation of MSNs in vitro. Replacement of wild-type iPSC-derived dopamine neurons (iPSC-DaNs) in the striatal microcircuit with those carrying the PD-related GBA-N370S mutation led to a depolarisation of resting membrane potential and an increase in rheobase in iPSC-MSNs, as well as a reduction in both voltage-gated sodium and potassium currents. Such deficits were resolved in late microcircuit cultures, and could be reversed in younger cultures with antagonism of protein kinase A activity in iPSC-MSNs. Taken together, our results highlight the unique utility of modelling striatal neurons in a modular physiological circuit to reveal mechanistic insights into GBA1 mutations in PD.</p> <p><strong>FILE DESCRIPTIONS</strong></p> <p>Source Data.xlsx: Tabular datasets plotted on main figures 1, 3, 4, 5 and 6.</p> <p>Supplementary Data.xlsx: Tabular datasets plotted on supplementary figures 1, 2, 3, 4, 5, 6, 7, 9, 10, 11, and 12.</p> <p>Key Resources Table.xlsx: Table containing key resources (primary and secondary antibodies, cell lines and software) used in this study.</p> <p>List of Primers.xlsx: Primers used in RT-qPCR.</p>
"The pathway of hyaluronic acid (HA) and its receptors (CD44, RHAMM) in the regulation of Rho GTPases and their effectors in an in vitro colorectal cancer model" ("Szlak kwasu hialuronowego (HA) i jego receptorów (CD44, RHAMM) w regulacji GTPaz Rho i ich efektorów w modelu raka jelita grubego in vitro"); NCN Miniatura 2022/06/X/NZ3/00848
<p>Results from Screening for "The pathway of hyaluronic acid (HA) and its receptors (CD44, RHAMM) in the regulation of Rho GTPases and their effectors in an in vitro colorectal cancer model" the project <strong>Miniatura</strong> (<strong>2022/06/X/NZ3/00848</strong>) funded by Polish <strong>National Science Centre (NCN)</strong></p> <p>Wyniki skriningu w projekcie "Szlak kwasu hialuronowego (HA) i jego receptorów (CD44, RHAMM) w regulacji GTPaz Rho i ich efektorów w modelu raka jelita grubego in vitro", <strong>Miniatura</strong> (<strong>2022/06/X/NZ3/00848</strong>) finansowanym przez <strong>Narodowe Centrum Nauki (NCN)</strong></p>
A new in vitro blood flow model for the realistic evaluation of antimicrobial surfaces
<p>Dataset to the publication</p> <p>A new <em>in vitro</em> blood flow model for the realistic evaluation of antimicrobial surfaces</p> <p>Juliane Valtin, Stephan Behrens, André Ruland, Florian Schmieder, Frank Sonntag, Lars D. Renner, Manfred F. Maitz, Carsten Werner</p> <p><em>Adv. Healthcare Mater.</em> 2023, 2301300. <a href="https://doi.org/10.1002/adhm.202301300">https://doi.org/10.1002/adhm.202301300</a></p>
Dataset related to article "New in silico models to predict in vitro micronucleus induction as marker of genotoxicity"
<p>The .txt file contains the dataset of the in silico model for genotoxicity as induction of micronuclei.</p> <p>The .doc file contains the descriptors of the models and the structural alerts.</p>
Supplementary Material: Evaluation of Cyanea capillata Sting Management Protocols Using Ex Vivo and In Vitro Envenomation Models
<p>Supplementary files for Doyle, T.K.; Headlam, J.L.; Wilcox, C.L.; MacLoughlin, E.; Yanagihara, A.A. Evaluation of <em>Cyanea capillata</em>Sting Management Protocols Using Ex Vivo and In Vitro Envenomation Models. <em>Toxins</em> <strong>2017</strong>, <em>9</em>, 215. Video S1: Vinegar Application to Gelatin-Adherent Cnidae</p>
Matlab code to calibrate a structured-PDE model to data from in vitro experiments
<p>Matlab code for the calibration of a PDE model of evolutionary dynamics of a well-mixed population of aggressive breast cancer cells from in vitro data on MCF7-sh-WISP2 cell line and bootstrapping for uncertainty quantification. For more details, see the associated publication: "Evolutionary dynamics of glucose-deprived cancer cells: insights from experimentally-informed mathematical modelling", by L. Almeida, J. Denis, N. Ferrand, T. Lorenzi, A. Prunet, M. Sabbah, C. Villa (corresponding author, author of code), 2023. In press in the journal of the Royal Society Interface.</p>
Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution
<p>Rawdata (images and flow cytometry) for the manuscript "Gastruloids as in vitro models of embryonic blood development with spatial and temporal resolution". <br> </p>
Data from: In vitro to in vivo extrapolation from three-dimensional hiPSC-derived cardiac microtissues and physiologically based pharmacokinetic modeling to inform next-generation arrythmia risk assessment
<p>Proarrhythmic cardiotoxicity remains a substantial barrier to drug development as well as a major global health challenge. <em>In vitro</em> human pluripotent stem cell-based new approach methodologies have been increasingly proposed and employed as alternatives to existing <em>in vitro</em> and <em>in vivo</em> models that do not accurately recapitulate human cardiac electrophysiology or cardiotoxicity risk. In this study, we expanded the capacity of our previously established three-dimensional human cardiac microtissue model to perform quantitative risk assessment by combining it with a physiologically based pharmacokinetic model, allowing a direct comparison of potentially harmful concentrations predicted <em>in vitro</em> to <em>in vivo</em> therapeutic levels. This approach enabled the measurement of concentration responses and margins of exposure for two physiologically relevant metrics of proarrhythmic risk (<em>i.e.</em>, action potential duration and triangulation assessed by optical mapping) across concentrations spanning three orders of magnitude. The combination of both metrics enabled accurate proarrhythmic risk assessment of four compounds with a range of known proarrhythmic risk profiles (<em>i.e., </em>quinidine, cisapride, ranolazine, and verapamil) and demonstrated close agreement with their known clinical effects. Action potential triangulation was found to be a more sensitive metric for predicting proarrhythmic risk associated with the primary mechanism of concern for pharmaceutical-induced fatal ventricular arrhythmias, delayed cardiac repolarization due to inhibition of the rapid delayed rectifier potassium channel, or hERG channel. This study advances human induced pluripotent stem cell-based three-dimensional cardiac tissue models as new approach methodologies that enable <em>in vitro</em> proarrhythmic risk assessment with high precision of quantitative metrics for understanding clinically relevant cardiotoxicity.</p>
Figure 4 in Dactylorhiza Fuchsii (Druce) Soó As A Model Object In In Vitro Culture Study For Development Of Terrestrial Orchids
Figure 4. Root of D. fuchsii growing in the Figure 5. Root of D. fuchsii one year after exposition in NBG: arrow indicates elaborate transplanting ex vitro: arrow indicates some coiled structures known as pelotons of peloton-like structure as they do within the orchid mycorrhizal fungi in the root cortex (section was cells (section was stained with Trypan Blue; bar stained with Trypan Blue; bar = 50 mm) = 50 mm).
Figure 1 in Dactylorhiza Fuchsii (Druce) Soó As A Model Object In In Vitro Culture Study For Development Of Terrestrial Orchids
Figure 1. Development of Dactylorhiza fuchsii (Druce) Soó in vitro conditions: A – beginning of morphogenesis after germination with development of protocorms, characteristic for orchids; B – initialization of rooting; C – sterile plantlets after exposition at 2 °C in the dark, simulating the natural dormancy period; D – plantlet before transplanting ex vitro (ro – root; tu – tuber).
Matlab code to calibrate a structured-PDE model to data from in vitro experiments
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Comparison of physiologically based pharmacokinetic modeling platforms for developmental neurotoxicity in vitro to in vivo extrapolation
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A dynamic in vitro model of Down Syndrome neurogenesis with Trisomy 21 gene dosage correction
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Data from: In vitro to in vivo extrapolation from three-dimensional hiPSC-derived cardiac microtissues and physiologically based pharmacokinetic modeling to inform next-generation arrythmia risk assessment
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Cross-disease integration of single-cell RNA sequencing data from lung myeloid cells reveals TAM signature in in vitro model
<p>Single cells from a 3D human cell-based model comprising tumor cell line-derived spheroids, cancer-associated fibroblasts and primary monocytes were dissociated and analyzed using scRNAseq. 4 monocyte donors were used in the 3D model, and 3 monocyte donors were used for 2D differentiation of macrophages.</p>
Agent-based model predicts that layered structure and 3D movement work synergistically to reduce bacterial load in 3D in vitro models of tuberculosis granuloma - Location Data
<p>This dataset is meant to be used with "Agent-based model predicts that layered structure and 3D movement work synergistically to reduce bacterial load in 3D in vitro models of tuberculosis granuloma - Results and Data". It provides spatial output data for 4 different setups (spheroid, traditional, 3d gravity, and traditional floating) of an agent-based model of <i>in vitro </i>tuberculosis infection models. </p>
Induced pluripotent stem cell-derived cardiomyocyte in vitro models: tissue fabrication protocols, assessment methods, and quantitative maturation metrics for benchmarking progress
<p>The advent of human induced pluripotent stem cells (hiPSCs) and techniques to differentiate cardiomyocytes from them has opened a viable path to creating <em>in vitro</em> models of normal and diseased hearts, accelerating more predictive drug screening and therapeutic strategies for cardiac pathologies. Currently, hiPSC-derived cardiomyocytes (hiPSC-CMs) are more similar to fetal than adult cardiomyocytes, leading many in the field to explore approaches to enhance cell and tissue maturation. There are over 2,000 studies utilizing hiPSC-CMs in models composed of various combinations of cell and extracellular matrix components, using a plethora of differentiation protocols, culture formats, and methods for quantifying cardiomyocyte function. To assess the current state of this rapidly growing area, we systematically analyzed 300 studies using hiPSC-CM models for their selection of hiPSC lines, hiPSC-CM differentiation protocols, types of <em>in vitro </em>models, maturation techniques, and metrics used to assess cardiomyocyte functionality and maturity. Here, we provide the data compiled from our analysis of these papers so others in the field can utilize it to inform their research.</p> <p>Based on this analysis, we highlight the diversity of, and current trends in, <em>in vitro</em> model designs and highlight the most common and promising practices for functional assessments. We further analyzed outputs spanning structural maturity, contractile function, electrophysiology, and gene expression and note field-wide improvements over time. Finally, we observe that a persistent lack of coordination amongst investigators is limiting the field's ability to benchmark and advance hiPSC-CM function against previous studies. We discuss opportunities to collectively pursue the common goal of hiPSC-CM model development, maturation, and assessment that we believe are critical to drive the entire community forward in engineering mature cardiac tissue.</p>
scRNA-seq dataset "A novel in vitro tubular model to recapitulate features of distal airways: the bronchioid"
<p>We provide a .Rds file of an annotated Seurat Object of scRNA-seq data of two bronchioid models derived from distinct donors after 21days of culture using 10x genomics 3' v3 chemistry. Raw data was processed using CellRanger v7.1.0. Cells were filtered based on detected UMIs (>2000) and fraction of mitochondrial counts (<10%).<br>Metadata annotations contain:<br>- Patient -> patient information for every cell (patient1 or patient2)<br>- nCount_RNA -> UMI counts per cell<br>- nFeature_RNA -> genes detected per cell<br>- percent.mt -> mitochondrial count fraction per cell<br>- seurat_clusters -> unsupervised clustering results using Louvain algorithm with resolution = 0.5<br>- Manual.Annotation -> Cell types annotated based on marker gene expression<br>- Celltypist.prediction -> Cell types predicted with CellTypist Python package<br>- Celltypist.prediction.ari -> Cell types predicted with CellTypist Python package, with harmonized names for comparison with manual annotation</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.