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9 results for “Large-scale single-cell”
Computational Analysis of Two-dimensional High-throughput Data from Large-scale RNAi Screens and Single-cell Transcriptomics
<p>This publication provides a singularity definition file to reproduce the computational environment along with the scripts to reproduce every figure or table in the revised manuscript using ZetaSuite Perl module and R package.</p> <p>First, generate a new folder and then download all the files into the folder.</p> <p>Then, uncompressed the files DataSets_part1.tar.gz,DataSets_part2.tar.gz,DataSets_part3.tar.gz,DataSets_part4.tar.gz, and scripts.tar.gz. within the folder.</p> <p>Next, move all the files in DataSets_part1 folder, DataSets_part2 folder,DataSets_part3 folder and DataSets_part4 folder to a new folder called DataSets.</p> <p>Finally, run the following scripts to generate the figures and tables in our manuscript.</p> <p>Regeneration of Figure2 and S2: singularity exec ZetaSuite.sif sh Figure2andS2.sh </p> <p>Regeneration of Figure3 and S3: singularity exec ZetaSuite.sif sh Figure3andS3.sh </p> <p>Regeneration of Figure4 and S4: singularity exec ZetaSuite.sif sh Figure4andS4.sh </p> <p>Regeneration of Figure5 and S5: singularity exec ZetaSuite.sif sh Figure5andS5.sh </p> <p>Regeneration of Figure6 and S6: singularity exec ZetaSuite.sif sh Figure6andS6.sh </p> <p>Regeneration of Figure7 and S7: singularity exec ZetaSuite.sif sh Figure7andS7.sh </p> <p> </p>
Code and data of "Uncovering disease-related multicellular pathway modules on large-scale single-cell transcriptomes with scPAFA"
<p>Code and data to reproduce the analyses and figures presented in "Uncovering disease-related multicellular pathway modules on large-scale single-cell transcriptomes with scPAFA"</p>
Data for - Tracking one-in-a-million: Large-scale benchmark for microbial single-cell tracking with experiment-aware robustness metrics
<p><strong>Large-scale Corynebacterium glutamicum data set with Segmentation and Tracking Annotation</strong></p> <p>We provide five time-lapse sequences with manually corrected segmentation and tracking annotations of growing <strong><em>C. glutamicum</em></strong> cultivations. The dataset contains more than 1.4 million cell observations in 29k cell tracks and 14k cell divisions. We provide videos of the annotations (videos.zip) and the dataset in <a href="http://celltrackingchallenge.net/datasets/">Cell Tracking Challenge</a> format (ctc_format.zip). In the videos, cell contours are rendered in yellow, cell links between frames are colored red and cell divisions, and their links are colored in blue.</p> <p><strong>Data Acquisition</strong></p> <p><strong><em>Corynebacterium glutamicum</em></strong> ATCC 13032 was cultivated in BHI-medium at 30°C in this study. From and overnight preculture, the main culture was inoculated the next day with a starting OD600 of 0.05 and grown at 120 rpm to a OD600 of 0.25. A chip was fabricated, according to <a href="https://doi.org/10.1039/D0LC00711K">(Täuber et al., 2020)</a>, and fixed to the microscope’s holder. The main culture cells were transferred to monolayer growth chambers (height = 720 nm) on the microfluidic chip. Flow through the microfluidic device was mediated by pressure driven pumps with a pressure of 100 mbar on the medium reservoir.</p> <p>The time-lapse phase contrast images of five monolayer growth chambers were taken every minute using an inverted microscope (Nikon Eclipse Ti2) with a 100x oil emersion objective and a DS-QI2 camera (Nikon) at 15 % relative DIA-illumination intensity and 100 ms exposure time. The spatial image resolution is 0.072 μm/px.</p>
Large-scale integration of single-cell transcriptomic data captures transitional progenitor states in mouse skeletal muscle regeneration
<p>Skeletal muscle repair is driven by the coordinated self-renewal and fusion of myogenic stem and progenitor cells. Single-cell gene expression analyses of myogenesis have been hampered by the poor sampling of rare and transient cell states that are critical for muscle repair, and do not inform the spatial context that is important for myogenic differentiation. Here, we demonstrate how large-scale integration of single-cell and spatial transcriptomic data can overcome these limitations. We created a single-cell transcriptomic dataset of mouse skeletal muscle by integration, consensus annotation, and analysis of 23 newly collected scRNAseq datasets and 88 publicly available single-cell (scRNAseq) and single-nucleus (snRNAseq) RNA-sequencing datasets. The resulting dataset includes more than 365,000 cells and spans a wide range of ages, injury, and repair conditions. Together, these data enabled identification of the predominant cell types in skeletal muscle, and resolved cell subtypes, including endothelial subtypes distinguished by vessel-type of origin, fibro/adipogenic progenitors defined by functional roles, and many distinct immune populations. The representation of different experimental conditions and the depth of transcriptome coverage enabled robust profiling of sparsely expressed genes. We built a densely sampled transcriptomic model of myogenesis, from stem cell quiescence to myofiber maturation and identified rare, transitional states of progenitor commitment and fusion that are poorly represented in individual datasets. We performed spatial RNA sequencing of mouse muscle at three time points after injury and used the integrated dataset as a reference to achieve a high-resolution, local deconvolution of cell subtypes. We also used the integrated dataset to explore ligand-receptor co-expression patterns and identify dynamic cell-cell interactions in muscle injury response. We provide a public web tool to enable interactive exploration and visualization of the data. Our work supports the utility of large-scale integration of single-cell transcriptomic data as a tool for biological discovery.</p>
Large-scale integration of single-cell transcriptomic data captures transitional progenitor states in mouse skeletal muscle regeneration
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Spatio-temporal landscape of mouse epididymal cells and specific mitochondria-rich segments defined by large-scale single-cell RNA-seq
GEO Series GSE159713. Mus musculus. 9 samples. Type: Expression profiling by high throughput sequencing.
Large-scale single cell mapping of the thymic stroma identifies a new thymic epithelial cell lineage [single-cell RNA-seq]
GEO Series GSE103967. Mus musculus. 135 samples. Type: Expression profiling by high throughput sequencing.
Large-scale single-cell analysis reveals critical immune characteristics of COVID-19 patients
GEO Series GSE158055. Homo sapiens. 284 samples. Type: Expression profiling by high throughput sequencing.
Single-cell RNA-seq data of large-scale bone calluses from skeletally immature and mature mice
GEO Series GSE250200. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.