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opencc-by-4.0Sep 2020View details →

Predicted Secreted Proteins for the Citrus Stem-End Rot Fungal Pathogen Lasiodiplodia theobromae CITRA15

<p>Lasiodiplodia theobromae (L. theobromae) belongs to a diverse genus of fungal pathogens associated with perennial tropical fruit plants worldwide. In citrus, L. theobromae causes stem-end rot (SER), a damaging postharvest disease that is aggravated when trees are also infected with the citrus greening bacterium, a Candidatus Liberibacter asiaticus. Due to the latent infection of L. theobromae during the preharvest stage, it is also difficult to control by chemical and physical treatment. We sequenced and assembled the first genome of isolate CITRA15 of L. theobromae obtained from citrus, providing a resource for future research on postharvest and preharvest disease management of citrus and other fruit crops and diagnostics. Here we want to share a set of i) 10,496 predicted coding gene sequences (cds) and ii) 10,496 predicted proteins and ii) 1,016 predicted secreted proteins using SignalP v2.0 and TMHMM v2.0.</p> <p> </p>

opennotspecifiedJun 2021View details →

FIGURE 2 in Morpho-phylogenetic evidence reveals Lasiodiplodia chiangraiensis sp. nov. (Botryosphaeriaceae) associated with woody hosts in northern Thailand

FIGURE 2. Lasiodiplodia chiangraiensis (MFLU 21-0003, holotype). a–c. Conidiomata on host surface. d. Section through conidiomata. e. Peridium. f. Ostiolar region with periphyses. g. Paraphyses. h–k. Conidia developing on conidiogenous cells. l–o. Hyaline, aseptate conidia. p. Germinating conidium. q, r. Colonies after 7 days on PDA (q from above, r from below). Scale bars: b = 500 μm, c = 200 μm, d–e = 10 μm, f = 20 μm, g–p = 10 μm.

opennotspecifiedJun 2021View details →

FIGURE 1 in Morpho-phylogenetic evidence reveals Lasiodiplodia chiangraiensis sp. nov. (Botryosphaeriaceae) associated with woody hosts in northern Thailand

FIGURE 1. Phylogenetic tree generated from maximum parsimony (MP) analysis based on combined ITS, tef and tub2 sequence data of Lasiodiplodia. Bootstrap values for maximum likelihood (ML) and maximum parsimony (MP) equal to or greater than 75% are placed above and below the branches, respectively. Branches with Bayesian posterior probabilities (BYPP) equal or greater than 0.95 are thickened. The new isolates are indicated in red and ex-type strains are in bold. The tree is rooted to Diplodia mutila (CMW 7060) and D. seriata (CBS 112555). The scale bar shows 20 changes.

Fig. 9. Compound 4 in Four undescribed ergostane-type steroids from Lasiodiplodia pseudotheobromae and their neuroprotective activity

Fig. 9. Compound 4 preserves mitochondrial function in glutamate-treated SH-SY5Y cell lines. Cell lines were measured using JC-1 staining by flow cytometry. Cell lines were assessed using H2DCF-DA staining and flow cytometry. Data are presented as the means ± S.D. (n = 3). ###p <0.001 vs. the control group (the no glutamate or compound 4-treated group); *p <0.05 and **p <0.001 vs. the glutamate-treated group.

Fig. 7 in Four undescribed ergostane-type steroids from Lasiodiplodia pseudotheobromae and their neuroprotective activity

Fig. 7. Effects of compounds 1–4 on the viability of Glu-treated SH-SY5Y cell lines. Compound 4 exerted a concentration dependent protective effect on SHSY5Y cell lines. Control represents the no glutamate or compounds-treated group. Data are presented as the means ± S.D. (n = 3). Data are presented as the means ± S.D. (n = 3). *p <0.05, **p <0.01 and ***p <0.001 vs. the glutamate-treated group.

Fig. 8. Compound 4 in Four undescribed ergostane-type steroids from Lasiodiplodia pseudotheobromae and their neuroprotective activity

Fig. 8. Compound 4 decreased glutamate-induced cellular apoptosis in SH-SY5Y cell lines. (A) Cellular morphological changes were observed by phase contrast microscopy. (B) Flow cytometry was applied to determine the apoptotic ratio after Annexin V-FITC/PI staining. The percentage of apoptotic cells was calculated in the bar chart. Data are presented as the means S.D. (n 3). ###p <0.001 vs. the control group (the no glutamate or compound 4-treated group); *p <0.05 and ± = ***p <0.001 vs. the glutamate-treated group.

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Photodynamic inactivation of Lasiodiplodia theobromae: lighting the way towards an environmentally friendly phytosanitary treatment

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Fig. 3 in Four undescribed ergostane-type steroids from Lasiodiplodia pseudotheobromae and their neuroprotective activity

Fig. 3. The key NOESY correlations of 1–4.

Fig. 2. The key 1H–1H in Four undescribed ergostane-type steroids from Lasiodiplodia pseudotheobromae and their neuroprotective activity

Fig. 2. The key 1H–1H COSY and HMBC correlations of 1–4.

Fig. 6 in Four undescribed ergostane-type steroids from Lasiodiplodia pseudotheobromae and their neuroprotective activity

Fig. 6. Experimental and calculated ECD spectra (200–400 nm) of 3 and 4.

Fig. 1 in Four undescribed ergostane-type steroids from Lasiodiplodia pseudotheobromae and their neuroprotective activity

Fig. 1. Structures of compounds 1–5.

Fig. 5. X in Four undescribed ergostane-type steroids from Lasiodiplodia pseudotheobromae and their neuroprotective activity

Fig. 5. X-ray crystal structures of 1 and 2.

Fig. 4 in Four undescribed ergostane-type steroids from Lasiodiplodia pseudotheobromae and their neuroprotective activity

Fig. 4. Candidate structures of compounds 1 and 2.