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opencc-by-4.0Mar 2022View details →

DNA damage and reticular stress in cytotoxicity and oncotic cell death of MCF-7 cells treated with fluopsin C

DNA damage and reticular stress in cytotoxicity and oncotic cell death of MCF-7 cells treated with fluopsin C

opencc-by-4.0May 2019View details →

#39 in MCF-7 and U87MG tumor models at different time points

Original CT and PET image files for compound #39 in MCF-7 and U87MG tumor models at different time points.

Fig. 7 in Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line

Fig. 7. Agarose gel electrophoresis of MCF-7 cell genomic DNA. 1, ladder marker; 2, negative control; 3, cyclophosphamide (CP)-treated; 4–7, ethanolic extracts of Mimosa caesalpiniifolia leaves (EEM) in different concentrations (5.0; 20.0; 160.0 and 320.0 μg/mL). The results are representative of three independent experiments carried out in the same conditions. DNA ladder formation indicates apoptosis as seen in lanes 3–7.

Fig. 6. MCF-7 in Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line

Fig. 6. MCF-7 cell death (%) after treatment with cyclophosphamide (CP, 550 μg/mL) and different concentrations (5.0–320.0 μg/mL) of the ethanolic extract of Mimosa caesalpiniifolia leaves (EEM) for 24 h, compared to the negative control cells (NC), estimated by the Fast green color dye exclusion. Results are expressed as mean ± SEM of three independent experiments. Different letters indicate significant differences (p <0.01) by the Tukey test. Inset: appearance of cells after fast green-hematoxylin–eosin staining; 1, living cell; 2, green dead cell. EEM treatment kills MCF-7 cells in a concentrationdependent manner.

Fig. 3 in Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line

Fig. 3. Morphology of MCF-7 cells stained with hematoxylin–eosin, after 24 and 48 h incubation. NC, negative control cells; CP, cells treated with 550 μg/mL cyclophosphamide; 5, 80 and 320, cells treated, respectively, with 5.0, 80.0 and 320.0 μg/mL ethanolic extract of Mimosa caenalpiniifolia leaves. All the pictures are typical of three independent experiments, each carried out under identical conditions. Bar = 5 μm. Arrow—nucleolus; R—rounding; CC—chromatin condensation.

Fig. 2. MCF-7 in Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line

Fig. 2. MCF-7 cell protein content decreasing (%), estimated by the sulforhodamine B assay, after treatment with cyclophosphamide (CP, 550 μg/mL) and different concentrations of the ethanolic extract of Mimosa caesalpiniifolia leaves (EEM 5.0 - 320.0 μg/mL) for 24 and 48 h. The results are expressed as mean ± SEM of three independent experiments. Different letters indicate significant differences (p <0.001) by the Tukey test. Protein content was calculated relative to the negative control and 320.0 μg/mL EEM produced the maximum effect.

Fig. 1 in Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line

Fig. 1. HPLC-DAD-ESI-MS analysis of the ethanolic extract of Mimosa caesalpiniifolia leaves. (A) UV 360 nm; (B) ESI-MS, base peak chromatogram, negative ion mode, m/z 100–1500. No additional peaks were detected when the UV trace was recorded at wavelengths down to 360 nm. Peaks assigned as m/z: 288.97, 318.00, and 576.77 were identified, respectively, as catechin, 2,3 dihydroquercetagetin, and procyanidin B2 [(epi)catechin–(epi)catechin)] (see structures).

Fig. 4. MCF-7 in Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line

Fig. 4. MCF-7 cell diameter (μm) after treatment with cyclophosphamide (CP, 550 μg/mL) and different concentrations (5.0–320.0 μg/mL) of the ethanolic extract of Mimosa caesalpiniifolia leaves (EEM) for 24 or 48 h, compared to the negative control cells (NC). Results are expressed as mean ± SEM of three independent experiments. Different letters indicate significant differences (p <0.01) by the Tukey test. Note cell-diameter reduction after treatment, in comparison to the negative control cells, thereby indicating EEM cytotoxicity.

opencc-zeroDec 2017View details →

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Data from: Metabolites of n-Butylparaben and iso-Butylparaben exhibit estrogenic properties in MCF-7 and T47D human breast cancer cell lines

Two oxidized metabolites of n-butylparaben (BuP) and iso-butylparaben (IsoBuP) discovered in human urine samples exhibit structural similarity to endogenous estrogens. We hypothesized that these metabolites bind to the human estrogen receptor (ER) and promote estrogen signaling. We tested this using models of ER-mediated cellular proliferation. The estrogenic properties of 3-hydroxy n-butyl 4-hydroxybenzoate (3OH) and 2-hydroxy iso-butyl 4-hydroxybenzoate (2OH) were determined using the ER-positive, estrogen-dependent human breast cancer cell lines MCF-7, and T47D. The 3OH metabolite induced cellular proliferation with EC50 of 8.2 µM in MCF-7 cells. The EC50 for 3OH in T47D cells could not be reached. The 2OH metabolite induced proliferation with EC50 of 2.2 µM and 43.0 µM in MCF-7 and T47D cells, respectively. The EC50 for the parental IsoBuP and BuP was 0.30 and 1.2 µM in MCF-7 cells, respectively. The expression of a pro-proliferative, estrogen-inducible gene (GREB1) was induced by these compounds and blocked by co-administration of an ER antagonist (ICI 182, 780), confirming the ER-dependence of these effects. The metabolites promoted significant ER-dependent transcriptional activity of an ERE-luciferase reporter construct at 10 and 20 µM for 2OH and 10 µM for 3OH. Computational docking studies showed that the paraben compounds exhibited the potential for favorable ligand-binding domain interactions with human ERα in a manner similar to known x-ray crystal structures of 17ß-estradiol in complex with ERα. We conclude that the hydroxylated metabolites of BuP and IsoBuP are weak estrogens and should be considered as additional components of potential endocrine disrupting effects upon paraben exposure.

opencc-zeroDec 2023View details →

Supplementary material 3 from: Khalaf WY, Elias RS, Raheem LA (2023) Design, synthesis and molecular docking study of coumarin pyrazoline derivatives against MCF-7 breast cancer cell line. Pharmacia 70(4): 1497-1492. https://doi.org/10.3897/pharmacia.70.e108670

Data synthesis compounds, physicochemical properties and identification of synthesis compound

opencc-zeroDec 2023View details →

Supplementary material 1 from: Khalaf WY, Elias RS, Raheem LA (2023) Design, synthesis and molecular docking study of coumarin pyrazoline derivatives against MCF-7 breast cancer cell line. Pharmacia 70(4): 1497-1492. https://doi.org/10.3897/pharmacia.70.e108670

Synthesis compounds

opencc-zeroDec 2023View details →

Supplementary material 2 from: Khalaf WY, Elias RS, Raheem LA (2023) Design, synthesis and molecular docking study of coumarin pyrazoline derivatives against MCF-7 breast cancer cell line. Pharmacia 70(4): 1497-1492. https://doi.org/10.3897/pharmacia.70.e108670

Docking study

opencc-by-4.0May 2019View details →

#41 in MCF-7 tumor model at different time points

Original PET and CT image files for compound #41 in MCF-7 tumor model at different time points

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Data from: Metabolites of n-Butylparaben and iso-Butylparaben exhibit estrogenic properties in MCF-7 and T47D human breast cancer cell lines

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publicMar 2018View details →

openGEO-OpenOct 2017View details →

ChIP-seq from MCF-7 (ENCSR801SWX)

GEO Series GSE105812. Homo sapiens. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.

openGEO-OpenMar 2019View details →

ChIP-seq from MCF-7 (ENCSR934JDG)

GEO Series GSE127640. Homo sapiens. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.

openGEO-OpenDec 2017View details →

MicroRNA-7-5p mediates the anti-oncogenic effect of hepatocyte growth factor in the MCF-10A mammary epithelial cell

GEO Series GSE102758. Homo sapiens. 2 samples. Type: Expression profiling by array.