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ShareScore release 0.7.1
Dataset results
17 results for “Macrophage uptake”
Increased uptake of silica nanoparticles in inflamed macrophages but not upon co-exposure to micron-sized particles
<p>Silica nanoparticles (NPs) are widely used in various industrial and biomedical applications. Little is known about the cellular uptake of co-exposed silica particles, as can be expected in our daily life. In addition, an inflamed microenvironment might affect a NP’s uptake and a cell’s physiological response. Herein, prestimulated mouse J774A.1 macrophages with bacterial lipopolysaccharide were post-exposed to micron- and nanosized silica particles, either alone or together, i.e., simultaneously or sequentially, for different time points. The results indicated a morphological change and increased expression of tumor necrosis factor alpha in lipopolysaccharide prestimulated cells, suggesting a M1-polarization phenotype. Confocal laser scanning microscopy revealed the intracellular accumulation and uptake of both particle types for all exposure conditions. A flow cytometry analysis showed an increased particle uptake in lipopolysaccharide prestimulated macrophages. However, no differences were observed in particle uptakes between single- and co-exposure conditions. We did not observe any colocalization between the two silica (SiO<sub>2</sub>) particles. However, there was a positive colocalization between lysosomes and nanosized silica but only a few colocalized events with micro-sized silica particles. This suggests differential intracellular localizations of silica particles in macrophages and a possible activation of distinct endocytic pathways. The results demonstrate that the cellular uptake of NPs is modulated in inflamed macrophages but not in the presence of micron-sized particles.</p>
Substrate stiffness reduces particle uptake by epithelial cells and macrophages in a size-dependent manner through mechanoregulation
<p>Cells continuously exert forces on their environment and respond to changes in mechanical forces by altering their behavior. Many pathologies such as cancer and fibrosis are hallmarked by dysregulation in the extracellular matrix, driving aberrant behavior through mechanotransduction pathways. We demonstrate that substrate stiffness can be used to regulate cellular endocytosis of particles in a size-dependent fashion. Culture of A549 epithelial cells and J774A.1 macrophages on polystyrene/glass (stiff) and polydimethylsiloxane (soft) substrates indicated that particle uptake is increased up to six times for A549 and two times for macrophages when cells are grown in softer environments. Furthermore, we altered surface characteristics through the attachment of submicron-sized particles as a method to locally engineer substrate stiffness and topography. To investigate the biomechanical changes which occurred within adherent epithelial cells, i.e. characterization of A549 cell spreading and focal adhesion maturation. Consequently, decreasing substrate rigidity and particle-based topography lead to a reduction of focal adhesion size. Moreover, expression levels of Yes-associated protein were found to correlate with the degree of particle endocytosis. A thorough appreciation of the mechanical cues may lead to improved solutions to optimize nanomedicine approaches for treatment of cancer and other diseases with abnormal mechanosignaling.</p>
Apoptotic Cell (AC) Uptake by Human Alveolar Macrophages (AM)
ClinicalTrials.gov study NCT01099410. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Exceptional Uptake, Limited Protein Expression: Liver Macrophages Lost in Translation of Synthetic mRNA
GEO Series GSE271200. Mus musculus. 35 samples. Type: Expression profiling by high throughput sequencing.
Inactivation of branched-chain amino acid uptake halts Staphylococcus aureus growth and induces bacterial quiescence within macrophages
GEO Series GSE235929. Homo sapiens; Staphylococcus aureus. 12 samples. Type: Expression profiling by high throughput sequencing.
TraSH analysis of Mycobacterium tuberculosis transposon mutants with reduced bodipy-palmitate uptake on the third day of resting bone marrow-derived macrophage infection
GEO Series GSE119753. Mycobacterium tuberculosis str. Erdman = ATCC 35801; Mycobacterium tuberculosis. 6 samples. Type: Expression profiling by array.
Complement protein C1q modulates macrophage activation and inflammasome activity during the uptake of apoptotic cells
GEO Series GSE30177. Homo sapiens. 18 samples. Type: Expression profiling by array.
Reduced Mitochondrial Calcium Uptake in Macrophages is a Major Driver of Inflammaging
GEO Series GSE228873. Mus musculus. 20 samples. Type: Expression profiling by high throughput sequencing.
Pathways for macrophage uptake of cell-free circular RNAs
GEO Series GSE264160. Mus musculus. 28 samples. Type: Expression profiling by high throughput sequencing; Other.
Tumor-associated macrophages derived from circulating inflammatory monocytes degrade collagen through cellular uptake
GEO Series GSE107053. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
A genome-wide screen in macrophages defines host genes regulating the uptake of Mycobacterium abscessus
GEO Series GSE221413. Mus musculus. 6 samples. Type: Other.
Identification of differentially expressed genes in Streptococcus agalactiae following phagocytic uptake and survival in THP-1 macrophage cells.
GEO Series GSE184709. Streptococcus agalactiae. 3 samples. Type: Expression profiling by high throughput sequencing.
Cancer EVP Uptake in Lung Interstitial Macrophages Enhances Vascular Permeability and Metastatic Potential (in vivo)
GEO Series GSE261138. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
Cancer EVP Uptake in Lung Interstitial Macrophages Enhances Vascular Permeability and Metastatic Potential (in vitro)
GEO Series GSE261137. Mus musculus. 10 samples. Type: Expression profiling by high throughput sequencing.
Macrophage Mannose receptor (MRC1) mediates uptake of dextran in murine bone-marrow derived macrophages via a receptor-mediated endocytic process
GEO Series GSE237551. Mus musculus; synthetic construct. 9 samples. Type: Other.
Particle uptake by macrophages triggers bifurcated transcriptional pathways that differentially regulate inflammation and lysosomal gene expression.
GEO Series GSE256362. Mus musculus. 157 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing.
Trem2 Promotes Foamy Macrophage Lipid Uptake and Survival in Atherosclerosis
GEO Series GSE231659. Mus musculus. 15 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.