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69 results for “Meiotic recombination”
Data from: Extensive recombination of a yeast diploid hybrid through meiotic reversion
In somatic cells, recombination between the homologous chromosomes followed by equational segregation leads to loss of heterozygosity events (LOH), allowing the expression of recessive alleles and the production of novel allele combinations that are potentially beneficial upon Darwinian selection. However, inter-homolog recombination in somatic cells is rare, thus reducing potential genetic variation. Here, we explored the property of S. cerevisiae to enter the meiotic developmental program, induce meiotic Spo11-dependent double-strand breaks genome-wide and return to mitotic growth, a process known as Return To Growth (RTG). Whole genome sequencing of 36 RTG strains derived from the hybrid S288c/SK1 diploid strain demonstrates that the RTGs are bona fide diploids with mosaic recombined genome, derived from either parental origin. Individual RTG genome-wide genotypes are comprised of 5 to 87 homozygous regions due to the loss of heterozygous (LOH) events of various lengths, varying between a few nucleotides up to several hundred kilobases. Furthermore, we show that reiteration of the RTG process shows incremental increases of homozygosity. Phenotype/genotype analysis of the RTG strains for the auxotrophic and arsenate resistance traits validates the potential of this procedure of genome diversification to rapidly map complex traits loci (QTLs) in diploid strains without undergoing sexual reproduction.
Data from: Male-mediated effects on female meiotic recombination
Recombination rates vary owing to an individual's genetic composition and/or its environmental condition. Yet, the effects of mating partner on recombination rates have not been considered. Here, I document a previously undescribed male-mediated effect on female recombination rates. After crossing females to males from different genetic backgrounds, I observed a significant difference in proportion of recombinant offspring based on the genetic background of the father (p=0.0292; 3 df; F=3.07). Genetic variation in male ability to affect recombination rate in their mates suggests the potential for sexual conflict on optimal proportion of recombinant offspring, perhaps leading to changes in population-level recombination rates with varying levels of sexual selection.
Data from: Mlh3 mutations in baker's yeast alter meiotic recombination outcomes by increasing noncrossover events genome-wide
Mlh1-Mlh3 is an endonuclease hypothesized to act in meiosis to resolve double Holliday junctions into crossovers. It also plays a minor role in eukaryotic DNA mismatch repair (MMR). To understand how Mlh1-Mlh3 functions in both meiosis and MMR, we analyzed in baker's yeast 60 new mlh3 alleles. Five alleles specifically disrupted MMR, whereas one (mlh3-32) specifically disrupted meiotic crossing over. Mlh1-mlh3 representatives for each class were purified and characterized. Both Mlh1-mlh3-32 (MMR+, crossover-) and Mlh1-mlh3-45 (MMR-, crossover+) displayed wild-type endonuclease activities in vitro. Msh2-Msh3, an MSH complex that acts with Mlh1-Mlh3 in MMR, stimulated the endonuclease activity of Mlh1-mlh3-32 but not Mlh1-mlh3-45, suggesting that Mlh1-mlh3-45 is defective in MSH interactions. Whole genome recombination maps were constructed for wild-type and MMR+ crossover-, MMR- crossover+, endonuclease defective and null mlh3 mutants in an S288c/YJM789 hybrid background. Compared to wild-type, all of the mlh3 mutants showed increases in the number of noncrossover events, consistent with recombination intermediates being resolved through alternative recombination pathways. Our observations provide a structure-function map for Mlh3 that reveals the importance of protein-protein interactions in regulating Mlh1-Mlh3's enzymatic activity. They also illustrate how defective meiotic components can alter the fate of meiotic recombination intermediates, providing new insights for how meiotic recombination pathways are regulated.
Data from: Controlling meiotic recombinational repair: specifying the roles of ZMMs, Sgs1 and Mus81/Mms4 in crossover formation
Crossovers (COs) play a critical role in ensuring proper alignment and segregation of homologous chromosomes during meiosis. How the cell balances recombination between CO vs. noncrossover (NCO) outcomes is not completely understood. Further lacking is what constrains the extent of DNA repair such that multiple events do not arise from a single double-strand break (DSB). Here, by interpreting signatures that result from recombination genome-wide, we find that synaptonemal complex proteins promote crossing over in distinct ways. Our results suggest that Zip3 (RNF212) promotes biased cutting of the double Holliday-junction (dHJ) intermediate whereas surprisingly Msh4 does not. Moreover, detailed examination of conversion tracts in sgs1 and mms4-md mutants reveal distinct aberrant recombination events involving multiple chromatid invasions. In sgs1 mutants, these multiple invasions are generally multichromatid involving 3–4 chromatids; in mms4-md mutants the multiple invasions preferentially resolve into one or two chromatids. Our analysis suggests that Mus81/Mms4 (Eme1), rather than just being a minor resolvase for COs is crucial for both COs and NCOs in preventing chromosome entanglements by removing 3′- flaps to promote second-end capture. Together our results force a reevaluation of how key recombination enzymes collaborate to specify the outcome of meiotic DNA repair.
Data from: Meiotic recombination shapes precision of pedigree- and marker-based estimates of inbreeding
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Data from: Male-mediated effects on female meiotic recombination
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Data from: Controlling meiotic recombinational repair: specifying the roles of ZMMs, Sgs1 and Mus81/Mms4 in crossover formation
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Data from: Extensive recombination of a yeast diploid hybrid through meiotic reversion
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Data from: Mlh3 mutations in baker's yeast alter meiotic recombination outcomes by increasing noncrossover events genome-wide
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Data from: Hybrid sterility locus on Chromosome X controls meiotic recombination rate in mouse
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Meiotic recombination cold spots in chromosomal cohesion sites
GEO Series GSE52863. Saccharomyces cerevisiae; Schizosaccharomyces pombe. 12 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Genome binding/occupancy profiling by genome tiling array.
HELLS and PRDM9 from a pioneer complex to open chromatin at meiotic recombination hotspots
GEO Series GSE135896. Mus musculus. 51 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Other.
Chromatin binding by HORMAD proteins regulates meiotic recombination initiation
GEO Series GSE225129. Saccharomyces cerevisiae. 46 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Other.
DNA cytosine methylation suppresses meiotic recombination at the sex-determining region (RNA-Seq)
GEO Series GSE245612. Chlamydomonas reinhardtii. 8 samples. Type: Expression profiling by high throughput sequencing.
Targeting meiotic recombination 11 (MRE11) nuclease for personalization of epithelial ovarian cancer therapy [2]
GEO Series GSE198648. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Extensive sex differences at the initiation of meiotic recombination
GEO Series GSE99921. Mus musculus. 22 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Other; Third-party reanalysis; Methylation profiling by high throughput sequencing.
Spo11-accessory proteins link DNA double-strand break sites to the chromosome axis in early meiotic recombination
GEO Series GSE29860. Saccharomyces cerevisiae. 34 samples. Type: Genome binding/occupancy profiling by genome tiling array.
The meiotic recombination activator PRDM9 trimethylates both H3K4 and H3K36 at recombination hotspots in vivo
GEO Series GSE76416. Mus musculus. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Replication origin selection organizes meiotic recombination
GEO Series GSE53921. Schizosaccharomyces pombe. 38 samples. Type: Genome binding/occupancy profiling by genome tiling array; Genome variation profiling by genome tiling array.
Targeting meiotic recombination 11 (MRE11) nuclease for personalization of epithelial ovarian cancer therapy
GEO Series GSE160540. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
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