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25 results for “Metagenomic profiling”
Introduction to Ancient Metagenomics Textbook (Edition 2025): Taxonomic Profiling, OTU Tables, and Visualisation
<p>Data and conda software environment file for the chapter 'Taxonomic Profiling, OTU Tables, and Visualisation' of the SPAAM Community's textbook: Introduction to Ancient Metagenomics (https://www.spaam-community.org/intro-to-ancient-metagenomics-book).</p>
MACREL software benchmark data set: Simulated metagenomes with sequencing quality, errors profile and abundance distributions derived from real samples
<p>These metagenomes were used in the benchmarking of FACS pipeline, and were designed after NGLess benchmark dataset (doi.org/10.5281/zenodo.2560288). Metagenomes were simulated with <a href="https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm">ART-bin-MountRainier-2016.06.05</a> using real abundance profiles (.abund files) available <a href="https://doi.org/10.5281/zenodo.2560288">elsewhere</a>, and <a href="http://progenomes1.embl.de/data/repGenomes/representatives.contigs.fasta.gz">proGenomes' representative contigs</a> as reference genomes. There are available metagenomes with 40, 60 and 80 M (million of reads) based in the reference genomes and abundances of the following samples:</p> <pre><code>SAMEA2466916 SAMEA2466953 SAMEA2466965 SAMEA2621107 SAMEA2621229 SAMEA2621247</code></pre> <p>To convert them from the CRAM format back to fastq files:</p> <pre><code> ## 1. converting from cram to bam format: samtools view -b -T refgenome.fa -o file.bam file.cram ## 2. sorting the bam file: samtools sort -n file.bam -o input_sorted.bam # sort reads by identifier-name (-n) ## 3. converting from bam to fastq format: bedtools bamtofastq -i input_sorted.bam -fq output_r1.fastq -fq2 output_r2.fastq </code></pre> <p> </p>
Supplementary data to accompany "phyloFlash: A pipeline for rapid SSU rRNA-targeted profiling of metagenomes"
<p>Usage examples of the phyloFlash pipeline applied to shotgun metagenomic data sets.</p> <p>The phyloFlash software is available from https://github.com/HRGV/phyloFlash. Examples were generated with phyloFlash v3.3b.</p>
SPAAM Summer School 2022: Introduction to Ancient Metagenomics - 3c Introduction to Taxonomic Profiling
<p>Teaching data for practical session: "3c Introduction to Taxonomic Profiling" of the 2022 SPAAM Summer School: Introduction to Ancient Metagenomics (Aug. 1-5 2022).</p> <p>See: <a href="https://spaam-community.github.io/wss-summer-school/#/2022/">https://spaam-community.github.io/wss-summer-school/#/2022/</a> or <a href="https://doi.org/10.5281/zenodo.6976711">https://doi.org/10.5281/zenodo.6976711</a> for slides.</p> <p>Once downloaded, run:</p> <pre><code>tar xvfz <session>.tar.gz</code></pre> <p> to decompress the data directory for the session.</p>
Supplementary Material for publication "Bifidobacteria Define Gut Microbiome Profiles of Golden Lion Tamarin (Leontopithecus rosalia} and Marmoset Callithrix sp. Metagenomic Shotgun Pools
<p>Supplementary Tables and Figure for the publication "Bifidobacteria Define Gut Microbiome Profiles of Golden Lion Tamarin <em>Leontopithecus rosalia</em> and Marmoset <em>Callithrix</em> sp. Metagenomic Shotgun Pools"</p>
SPAAM Summer School 2022: Introduction to Ancient Metagenomics - 5c Introduction to Functional Profiling
<p>Teaching data for practical session: "5c Introduction to Functional Profiling" of the 2022 SPAAM Summer School: Introduction to Ancient Metagenomics (Aug. 1-5 2022).</p> <p>See: <a href="https://spaam-community.github.io/wss-summer-school/#/2022/">https://spaam-community.github.io/wss-summer-school/#/2022/</a> or <a href="https://doi.org/10.5281/zenodo.6976711">https://doi.org/10.5281/zenodo.6976711</a> for slides.</p> <p>Once downloaded, run:</p> <pre><code>tar xvfz <session>.tar.gz</code></pre> <p> to decompress the data directory for the session.</p> <p>This updated version changes the conda environment due to versioning conflicts.</p>
Supplementary data (simulated metagenome set 2) to accompany "phyloFlash – Rapid SSU rRNA profiling and targeted assembly from metagenomes"
<p>Comparison of SSU rRNA read extraction and targeted assembly from simulated shotgun metagenome of closely related Bacteorides strains.</p> <p>The phyloFlash software is available from https://github.com/HRGV/phyloFlash. Examples were generated with phyloFlash v3.3b.</p>
Supplementary data (simulated metagenome set 3) to accompany "phyloFlash – Rapid SSU rRNA profiling and targeted assembly from metagenomes"
<p>Comparison of SSU rRNA read extraction and targeted assembly from simulated shotgun metagenome of closely related Bacteorides strains.</p> <p>The phyloFlash software is available from https://github.com/HRGV/phyloFlash. Examples were generated with phyloFlash v3.3b.</p>
Culex pipiens merged anvi'o profiles from midgut and ovary metagenomes
<p>Anvi’o merged profile databases for <em>Culex pipiens</em> midgut and ovary samples. </p>
Benchmarking datasets used in the manuscript "Strain-level metagenomic profiling using pangenome graphs with PanTax"
Open the record for dataset details and reuse information.
Prokaryotic gene catalog, prokaryotic Metagenome-Assembled Genomes (MAGs) and taxonomic profiling of metagenomic data of NEREA Augmented Observatory
<p>The NEREA_metaG directory is dedicated to the in-depth analysis of NEREA microbial communities using metagenomic sequencing data. </p> <p><strong>Gene catalog:</strong> This directory contains the gene catalog compiled from metagenomic data, which includes: Protein and nucleotide sequence files for genes; Cluster files grouping similar genes; Annotation files mapping genes to KEGG pathways; Normalized gene abundance profiles.</p> <div><strong>MAGs:</strong> Directory for Metagenome-Assembled Genomes (MAGs). It contains comprehensive annotation files for the MAGs, providing insights into gene functions, metabolic pathways, and other genomic features. It also contains the individual MAGs categorized by sample origin. Each MAG is stored in a compressed FASTA format.</div> <p><strong>mOTUs</strong>: Contains files related to microbial taxonomic units identified and quantified using the mOTUs profiler. </p>
Large-scale metagenomic analysis of oral microbiomes reveals markers for autism spectrum disorders, MetaPhlAn 3 profiles
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The Effect of Probiotic Lactobacillus Reuteri Prodentis Consumption on Gingival Crevicular Fluid Inflammatory Response and Metagenomic Profiles of Oral Microbiome in Orthodontic Patients.
ClinicalTrials.gov study NCT04847960. IPD Sharing: Not stated. Countries: 1. Publications: 24.
Supplementary data (simulated metagenome set 1) to accompany "phyloFlash – Rapid SSU rRNA profiling and targeted assembly from metagenomes"
<p>Comparison of SSU rRNA read extraction and targeted assembly from simulated shotgun metagenome of divergent bacterial species.</p> <p>The phyloFlash software is available from https://github.com/HRGV/phyloFlash. Examples were generated with phyloFlash v3.3b.</p>
Supplementary data (comparison of multiple metagenomes) to accompany "phyloFlash – Rapid SSU rRNA profiling and targeted assembly from metagenomes"
<p>Usage example for phyloFlash, comparison of multiple metagenomes by SSU rRNA taxonomic profile. </p> <p>The phyloFlash software is available from https://github.com/HRGV/phyloFlash. Examples were generated with phyloFlash v3.3b.</p>
Supplementary data (low-diversity metagenome and reference database completeness) to accompany "phyloFlash – Rapid SSU rRNA profiling and targeted assembly from metagenomes"
<p>Comparison of phyloFlash and Matam on low-diversity platyhelminth metagenome, showing effect of reference database completeness on results. </p> <p>The phyloFlash software is available from https://github.com/HRGV/phyloFlash. Examples were generated with phyloFlash v3.3b.</p>
Supplementary data (Tara Oceans metagenomes) to accompany "phyloFlash – Rapid SSU rRNA profiling and targeted assembly from metagenomes"
<p>Comparison of SSU rRNA read extraction and targeted assembly by phyloFlash and Matam from environmental metagenomes from the Tara Oceans dataset.</p> <p>The phyloFlash software is available from https://github.com/HRGV/phyloFlash. Examples were generated with phyloFlash v3.3b.</p>
Metagenomics reveals sex differences in murine fecal microbiota profile induced by chronic alcohol consumption
Open the record for dataset details and reuse information.
Whole Metagenome Profiles of Particulates Collected from the International Space Station
The microbial composition of the International Space Station (ISS) environment is of critical interest due to potential impact of its constituents on human health and operational mission success. This study examined the whole metagenome of ISS microbes at both a species- and gene-level resolution. Air filter and dust samples from the ISS were analyzed and compared to samples collected in a terrestrial cleanroom environment. Samples were collected from ISS and cleanroom environments and treated to examine DNA from total versus viable populations. Microbial genes relevant to human health such as antimicrobial resistance and virulence genes were quantified.
Microbial Profiling in Pockets Related to Chronic Periodontitis Patients Using 16s RNA Metagenomics Sequencing
ClinicalTrials.gov study NCT04425343. IPD Sharing: NO. Countries: 1. Publications: 0.
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Allen Brain Atlas
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.