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45 results for “Metarhizium”
Fig. 1 in Neem oil increases the efficiency of the entomopathogenic fungus Metarhizium anisopliae for the control of Aedes aegypti (Diptera: Culicidae) larvae
Fig. 1 Dacls survcval curves of Aedes aegypti larvae exposed to dcfferent concentratcons of neem ocl. Note: Results are the means (± SE) of three expercments for each treatment wcth 30 cnsects used per treatment for each expercment
Fig. 2 in Neem oil increases the efficiency of the entomopathogenic fungus Metarhizium anisopliae for the control of Aedes aegypti (Diptera: Culicidae) larvae
Fig. 2 Dacls survcval curves of Aedes aegypti larvae exposed to dcfferent concentratcons of Metarhizium anisopliae concdca. Note: Results are the means (± SE) of three expercments for each treatment wcth 30 cnsects used per treatment for each expercment
Figure 2. A in Susceptibility of Agriotes spp. larvae (Coleoptera: Elateridae) to stress-and-kill strategies using spinosad and the entomopathogenic fungus Metarhizium brunneum
Figure 2. A: MetarhIzIum brunneum strain ART2825 growth in the tracheae of AgrIotes obscurus 22 days posttreatment. B: ART2825 fungal colonization in the integument of A. obscurus 22 days posttreatment.
Figure 3. A in Susceptibility of Agriotes spp. larvae (Coleoptera: Elateridae) to stress-and-kill strategies using spinosad and the entomopathogenic fungus Metarhizium brunneum
Figure 3. A: Sporulation of MetarhIzIum brunneum strain 16P on AgrIotes sordIdus 21 days posttreatment (zoom ×6.7). B: M. brunneum strain 16P primary and secondary fungal growth, with melanotic spots (black arrow) on A. sordIdus (×6.7). C: ART2825 fungal growth on the cuticle of A. obscurus 22 days posttreatment. D: Fungal growth on the cuticle of A. obscurus, which could correspond to the secondary growth on the sclerites.
Figure 1. A in Susceptibility of Agriotes spp. larvae (Coleoptera: Elateridae) to stress-and-kill strategies using spinosad and the entomopathogenic fungus Metarhizium brunneum
Figure 1. A: Leg of AgrIotes obscurus exposed to MetarhIzIum brunneum strain ART2825 48 h posttreatment. B: intersegment area of A. obscurus exposed to M. brunneum strain F52 36 h posttreatment, C: Depression at the base of a setae of A. obscurus with F52 conidia 24 h posttreatment, D: Melanization on A. sordIdus exposed to M. brunneum strain 16P 21 days posttreatment (zoom x 6.7).
Fig. 2 in Evaluation of isolates of entomopathogenic fungi in the genera Metarhizium, Beauveria, and Isaria, and their virulence to Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)
Fig. 2. Daily mean survival curves for adult Thaumastocoris peregrinus exposed to entomopathogenic fungal isolates of the genera Beauveria, Metarhizium, and Isaria for a period of 10 d. The horizontal dotted line indicates insect mortality of 50%.
Fig. 1 in Evaluation of isolates of entomopathogenic fungi in the genera Metarhizium, Beauveria, and Isaria, and their virulence to Thaumastocoris peregrinus (Hemiptera: Thaumastocoridae)
Fig. 1. Percentage of confirmed mortality of Thaumastocoris peregrinus, treat- ed with suspensions of 1 × 108 conidia per mL of entomopathogenic fungal isolates. Different letters above the bars indicate differences between treatments by Tukey's test (P <0.05). Bars represent the error ± 1 × SEM (df = 100).
Fig. 1 in Isolation of Metarhizium guizhouense and Metarhizium robertsii strains from soil-exposed Amblyomma americanum (Acarina: Ixodidae) from northwest Arkansas, USA
Fig. 1. Metarhizium robertsii (A-C, E) and Metarhizium guizhouense (D, F) from Amblyomma americanum ticks collected from northwest Arkansas (Washington County). (A) Sporulating M. robertsii (Savoy P2AM1/ARSEF 14332) growing on an infected adult male tick. (B) Inset of infected tick showing sporulating conidia in addition to mouthparts and coxal spurs diagnostic of A. americanum. (C, D) Metarhizium robertsii (Savoy P2AM1/ARSEF 14432) and M. guizhouense (West Fork P9N2/ ARSEF 14330), respectively, 10 d old colony on Sabouraud Dextrose Agar (plate diam = 60 mm). (E, F) Conidia of M. robertsii (Savoy P2AM1/ARSEF 14332) and M. guizhouense (West Fork P9N2/ARSEF 14330), respectively, viewed at 200× magnification (scale = 20 µm). Photos: Austin Goldsmith (A, B) and Louela Castrillo (C-F).
Fig. 1 in Occurrence of Metarhizium rileyi (Farlow) Kepler, S. A. Rehner & Humber in Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae) and Trichoplusia ni Hübner (Lepidoptera: Noctuidae) larvae in Tamaulipas and Veracruz, Mexico
Fig. 1. Larvae of (A) Anticarsia gemmatalis and (B) Trichoplusia ni infected by Metarhizium rileyi, collected from soybean plants in the states of Tamaulipas and Veracruz, Mexico; (C) Conidiophores of M. rileyi at 100× magnification and dyed with cotton blue; (D) Spores of M. rileyi at 100× magnification and dyed with cotton blue.
Survival data of Agriotes obscurus beetles exposed to Metarhizium brunneum sporulating cadavers
<p>The dataset contains survival and mycosis data of <em>Agriotes obscurus</em> male beetles exposed to <em>Metarhizium brunneum</em> sporulating cadavers, or soil contaminated by sporulating cadavers. </p>
RNAseq, virulence, and phylogenetics studies of the gene easR of Metarhizium brunneum
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Self-grooming behavior of <em>Aedes aegypti</em> exposed to <em>Metarhizium humberi</em> formulation
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Single Nucleotide Polymorphisms (SNPs) data of Metarhizium species for Taxonomic Elucidation of M. anisopliae sensu lato
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FIGURE 1. Phylogenetic relationship between Metarhizium synnematis GZUHXCHL12 in Delimitation of a novel member of genus Metarhizium (Clavicipitaceae) by phylogenetic and network analysis
FIGURE 1. Phylogenetic relationship between Metarhizium synnematis GZUHXCHL12 and its allies based on ITS-5.8S rDNA sequence data. Bootstrap values (1,000 replicates) are indicated above the nodes.
FIGURE 3 in Delimitation of a novel member of genus Metarhizium (Clavicipitaceae) by phylogenetic and network analysis
FIGURE 3. Metarhizium synnematis (holotype) A. Synnema on a lepidopteran cocoon. B. Upper part of a synnema. C. Phialides covering the surface of the synnema in a hymenium with conidia aggregating in sticky masses. D. Phialides. E. Conidia. Scale bars: A = 10 mm, B = 100 μm, C–E = 10 μm.
FIGURE 2 in Delimitation of a novel member of genus Metarhizium (Clavicipitaceae) by phylogenetic and network analysis
FIGURE 2. Minimum spanning network displaying the relationship among Metarhizium synnematis, Neotyphodium sp., Akanthomyces sp. and Metarhizium sp.
Chemical analyses and insect interactions of an easO mutant of Metarhizium brunneum
<p>Several fungi, including the plant root symbiont and insect pathogen <i>Metarhizium brunneum</i>, produce lysergic acid amides via a branch of the ergot alkaloid pathway. Lysergic acid amides include important pharmaceuticals and pharmaceutical lead compounds and have potential ecological significance, making knowledge of their biosynthesis relevant. Many steps in the biosynthesis of lysergic acid amides have been determined, but terminal steps in the synthesis of lysergic acid α-hydroxyethylamide (LAH)––by far the most abundant lysergic acid amide in <i>M. brunneum</i>––are unknown. Ergot alkaloid synthesis (<i>eas</i>) genes are clustered in the genomes of fungi that produce these compounds, and the <i>eas</i> clusters of LAH producers contain two uncharacterized genes (<i>easO</i> and <i>easP</i>) not found in fungi that do not produce LAH. Knockout of <i>easO</i> via a CRISPR-Cas9 approach eliminated LAH and resulted in accumulation of alternate lysergic acid amides lysergyl-alanine and ergonovine. Despite the elimination of LAH, the total concentration of lysergic acid derivatives was not affected significantly by the mutation. Complementation with a wild-type allele of <i>easO</i> restored the ability to synthesize LAH. Substrate feeding studies indicated that neither lysergyl-alanine nor ergonovine were substrates for the product of <i>easO</i> (EasO). EasO had structural similarity to Baeyer-Villiger monooxygenases (BVMOs), and labeling studies with deuterated alanine supported a role for a BVMO in LAH biosynthesis. The <i>easO</i> knockout had reduced virulence to larvae of the insect <i>Galleria mellonella</i>, indicating that LAH contributes to virulence of <i>M. brunneum</i> on insects and that LAH has biological activities different from ergonovine and lysergyl-alanine.</p>
FIGURE. Metarhizium guizhouense (GMB0010) (new host record). a, b. Fungus on stick insects (Phasmatodea) c, d. Green mycelium and sporulating conidiophores covered on the surface of inscect. e, f, g. Conidiophores h, i. Conidia on insect host. Scale bars: a, b = 5 mm, c = 2 mm, d = 500 μm, j–r = 10 μm, e–i = 5μm in Yunnan-Guizhou Plateau: a mycological hotspot
FIGURE. Metarhizium guizhouense (GMB0010) (new host record). a, b. Fungus on stick insects (Phasmatodea) c, d. Green mycelium and sporulating conidiophores covered on the surface of inscect. e, f, g. Conidiophores h, i. Conidia on insect host. Scale bars: a, b = 5 mm, c = 2 mm, d = 500 μm, j–r = 10 μm, e–i = 5μm
FIGURE 2 in Metarhizium macrosemiae sp. nov. and the anamorph of M. guniujiangense on adult cicada from Guniujiang Nature Preserve, southeastern China
FIGURE 2. Metarhizium macrosemiae (GNJ20200812-01, culture ex-type RCEF 6696). A. Fungus on adult cicada. B. Macroconidia on insect host. C. Microconidia on insect host. D–E. Colonies on SDAY/4. F. Conidiophores bearing phialides and conidia on SDAY/4. G. Macro- and microconidia on SDAY/4. H–I. Colonies on PDA. J. Conidiophores bearing phialides and conidia on PDA. K. Macro- and microconidia on PDA. L–M. Colonies on OA. N. Conidiophores bearing phialides and conidia on OA. O. Macro- and microconidia on OA. Scale bars: A = 10 mm, D–E, H–I, L–M = 15 mm, B–C, F–G, J–K, N–O = 10 μm.
FIGURE 1 in Metarhizium macrosemiae sp. nov. and the anamorph of M. guniujiangense on adult cicada from Guniujiang Nature Preserve, southeastern China
FIGURE 1. Phylogenetic relationships of Metarhizium and related genera in the Clavicipitaceae obtained from the combined SSU, LSU, TEF, RPB1 and RPB2 sequences based on maximum parsimony, maximum likelihood and Bayesian analyses. Numbers on the nodes are MP bootstrap values / ML bootstrap values / Bayesian posterior probability above 70% (MPBS-MLBS) or 0.7 (BIPP). Bold lines indicate support for the three analyses was 100% (MPBS-MLBS) or 1.0 (BIPP). *Represents the ex-type strain.
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