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16
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ShareScore release 0.9.0
Dataset results
16 results for “Microtubule dynamics”
Live-cell imaging of LLC-PK1 cells microtubule dynamics
<p>Stable LLC-PK1 cell line (ATCC:CL-101) was generated and provided by Michael W. Davidson [1,2]. Using a Zeiss Celldiscoverer 7 microscope with a 100X/1.47 NA oil-immersion objective (Plan-Apochromat, Zeiss) and a sCMOS sensor (Hamamatsu, ORCA-Fusion, C15440-20UP), cultured LLC-PK1 cells expressing mEmerald-EB3 were imaged with a 43 nm pixel size. Exposure time was 100 ms, with each frame being captured every two seconds. During acquisition, temperature and CO2 control was set to 37°C and 5%, respectively. mEmerald-EB3 was excited using a 488 nm laser at 1% power, with a FITC filter for fluorescence collection. Acquisition software ZEN 3.2 (blue edition) was used for the imaging protocol.</p> <p>1. Rizzo, M. A., Davidson, M. W., & Piston, D. W. (2009). Fluorescent protein tracking and detection: fluorescent protein structure and color variants. Cold Spring Harbor Protocols, 2009(12), pdb-top63.</p> <p>2. Huang, F., Hartwich, T., Rivera-Molina, F. et al. Video-rate nanoscopy using sCMOS camera–specific single-molecule localization algorithms. Nat Methods 10, 653–658 (2013).</p>
The non-mitotic role of HMMR in regulating the localization of TPX2 and the dynamics of microtubules in neurons
<p>A functional nervous system is built upon the proper morphogenesis of neurons to establish the intricate connection between them. The microtubule cytoskeleton is known to play various essential roles in this morphogenetic process. While many microtubule-associated proteins (MAPs) have been demonstrated to participate in neuronal morphogenesis, the function of many more remains to be determined. This study focuses on a MAP called HMMR in mice, which was originally identified as a hyaluronan binding protein and later found to possess microtubule and centrosome binding capacity. HMMR exhibits high abundance on neuronal microtubules and altering the level of HMMR significantly affects the morphology of neurons. Instead of confining to the centrosome(s) like cells in mitosis, HMMR localizes to microtubules along axons and dendrites. Furthermore, transiently expressing HMMR enhances the stability of neuronal microtubules and increases the formation frequency of growing microtubules along the neurites. HMMR regulates the microtubule localization of a non-centrosomal microtubule nucleator TPX2 along the neurite, offering an explanation for how HMMR contributes to the promotion of growing microtubules. This study sheds light on how progenitor cells utilize proteins involved in mitosis for non-mitotic functions.</p>
The non-mitotic role of HMMR in regulating the localization of TPX2 and the dynamics of microtubules in neurons
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Data for the publication "Mechanical communication within the microtubule through network-based analysis of tubulin dynamics"
<p>Repository containing all the necessary data to replicate the study "Mechanical communication within the microtubule through network-based analysis of tubulin dynamics", published in Biomechanics and Modeling in Mechanobiology (https://doi.org/10.1007/s10237-023-01792-5).</p>
Spastin locally amplifies microtubule dynamics to pattern the axon for presynaptic cargo delivery.
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Active nematic order and dynamic lane formation of microtubules driven by membrane-bound diffusing motors
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Tubulin mRNA stability is sensitive to change in microtubule dynamics caused by multiple physiological and toxic cues
GEO Series GSE128414. Homo sapiens. 31 samples. Type: Expression profiling by high throughput sequencing.
RNA helicase MOV10 suppresses fear memory and dendritic arborization and regulates microtubule dynamics in hippocampal neurons [eCLIP-seq]
GEO Series GSE278282. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
6-acetoxy-anopterine: A novel structure class of mitotic inhibitor disrupting microtubule dynamics in prostate cancer cells
GEO Series GSE81277. Homo sapiens. 10 samples. Type: Expression profiling by array.
Dysregulation of microtubule dynamics caused by LOXL3 knockout triggers mitosis catastrophe and senescence in U87MG and U251 cells
GEO Series GSE288138. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
CircZNF609 regulates microtubule dynamics ad tumorigenicity by sustaining CKAP5 protein levels.
GEO Series GSE174172. Homo sapiens. 4 samples. Type: Expression profiling by high throughput sequencing; Other.
The integrated stress response protects microtubule dynamics to recover from proteotoxic stress
GEO Series GSE193945. Mus musculus. 31 samples. Type: Other; Expression profiling by high throughput sequencing.
FOXJ1 regulates microtubule dynamics in nonciliated cells and is a mediator of taxane resistance
GEO Series GSE276579. Homo sapiens. 25 samples. Type: Expression profiling by high throughput sequencing.
RNA helicase MOV10 suppresses fear memory and dendritic arborization and regulates microtubule dynamics in hippocampal neurons [RNA-seq]
GEO Series GSE232771. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
HAT cofactor TRRAP modulates microtubule dynamics via SP1 signaling to prevent neurodegeneration
GEO Series GSE131028. Mus musculus. 8 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Acetyl-CoA carboxylase inhibition regulates microtubule dynamics and intracellular transport in cystic fibrosis epithelial cells
GEO Series GSE118567. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.