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7 results for “Morus notabilis”
Fig. 7 in Genome-wide identification and characterization of genes involved in melatonin biosynthesis in Morus notabilis (wild mulberry)
Fig. 7. Biosynthetic pathway of melatonin. The black arrows identify the pathway in herbaceous and woody plants while the red arrows identify the pathway in woody plants. The enzymes of the respective steps are as follows: TDC; T5H; SNAT; ASMT; and COMT. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 5 in Genome-wide identification and characterization of genes involved in melatonin biosynthesis in Morus notabilis (wild mulberry)
Fig. 5. Purification and enzymatic analyses of recombinant His-tagged MnASMT12. A: Purification of recombinant His-tagged MnASMT12 (lane1: Protein Marker; lane2: pCold TF (+IPTG); lane3: recombinant protein (-IPTG); lane4: recombinant protein (+IPTG); lane5: purified protein). B: Mass spectrum of compounds detected using UPLC-MS/MS. a: melatonin standard; b: Chromatogram of N-acetylserotonin catalyzed by MnASMT12. C: Determination of Km and Vmax of MnASMT12 for Nacetylserotonin. MnASMT12 (1.0 μg) was co-incubated with different concentrations of N-acetylserotonin for 20 min at 37 ◦C. D: Mass spectrum of compounds detected using UPLC-MS/MS. a: 5-methoxytryptamine standard; b: Chromatogram of serotonin catalyzed by MnASMT12. E: Determination of Km and Vmax of MnASMT12 for serotonin. MnASMT12 (1.0 μg) was co-incubated with different concentrations of serotonin for 20 min at 37 ◦C. " IPTG" stand without addition of 1 mM IPTG to the E. coli BL21 (DE3) culture, "+IPTG" stand addition of 1 mM IPTG to the E. coli BL21 (DE3) culture. "TF" is Trigger Factor, a prokaryotic ribosomerelated molecular chaperone that facilitates the translation and folding of peptides. "*" indicates Trigger factor.
Fig. 3 in Genome-wide identification and characterization of genes involved in melatonin biosynthesis in Morus notabilis (wild mulberry)
Fig. 3. Purification and enzymatic analyses of recombinant His-tagged MnTDC and recombinant His-tagged MnT5H2. A: Purification of recombinant His-tagged MnTDC (lane1: Protein Marker; lane2: pCold TF (+IPTG); lane3: recombinant protein (-IPTG); lane4: recombinant protein (+IPTG); lane5: purified protein). B: Mass spectrum of compounds detected using UPLC-MS/MS. a: tryptamine standard; b: Chromatogram of tryptophan catalyzed by MnTDC. C: Determination of Km and V of MnTDC for tryptophan. The MnTDC (1.0 μg) was co-incubated with different concentrations of tryptophan for 20 min at 37 ◦C. Trp: tryptophan; Try: max tryptamine. D: Purification of recombinant His-tagged MnT5H2 (lane1: Protein Marker; lane2: pCold TF (+IPTG); lane3: recombinant protein (-IPTG) lane4: recombinant protein (+IPTG); lane5: purified protein). E: Mass spectrum of compounds detected using UPLC-MS/MS. a: serotonin standard; b: Chromatogram of tryptamine catalyzed by MnT5H2. F: Determination of Km and Vmax of MnT5H2 for tryptamine. The MnT5H2 (1.0 μg) was co-incubated with different concentrations of tryptamine for 20 min at 28 ◦C. Ser: serotonin. " IPTG" stand without addition of 1 mM IPTG to the E. coli BL21 (DE3) culture, "+IPTG" stand addition of 1 mM IPTG to the E. coli BL21 (DE3) culture. "TF" is Trigger Factor, a prokaryotic ribosome-related molecular chaperone that facilitates the translation and folding of peptides. "*" indicates Trigger factor.
Fig. 4 in Genome-wide identification and characterization of genes involved in melatonin biosynthesis in Morus notabilis (wild mulberry)
Fig. 4. Purification and enzymatic analyses of recombinant His-tagged MnSNAT5. A: Purification of recombinant His-tagged MnSNAT5 (lane1: Protein Marker; lane2: pCold TF (+IPTG); lane3: recombinant (-IPTG); lane4: recombinant (+IPTG); lane5: purified protein). B: Mass spectrum of compounds detected using UPLCMS/MS. a: N-acetylserotonin standard; b: Chromatogram of serotonin catalyzed by MnSNAT5. C: Determination of Km and Vmax of MnSNAT5 for serotonin. MnSNAT5 (1.0 μg) was co-incubated with different concentrations of serotonin for 20 min at 37 ◦C. D: Mass spectrum of compounds detected using UPLC-MS/MS. a: standard, b: Chromatogram of 5-methoxytryptamine catalyzed by MnSNAT5. E: Determination of Km and Vmax of MnSNAT5 for 5-methoxytryptamine. MnSNAT5 (1.0 μg) was coincubated with different concentrations of 5-methoxytryptamine for 20 min at 37 ◦C. " IPTG" stand without addition of 1 mM IPTG to the E. coli BL21 (DE3) culture, "+IPTG" stand addition of 1 mM IPTG to the E. coli BL21 (DE3) culture. "TF" is Trigger Factor, a prokaryotic ribosome-related molecular chaperone that facilitates the translation and folding of peptides. "*" indicates Trigger factor. NAS: N-acetylserotonin; 5-MT: 5-methoxytryptamine; Mel: melatonin.
Fig. 2 in Genome-wide identification and characterization of genes involved in melatonin biosynthesis in Morus notabilis (wild mulberry)
Fig. 2. Expression analysis of genes involved in melatonin biosynthesis. A: Heat map showing tissue-specific expression profiles of melatonin biosynthetic genes in M. notabilis, including MnTDC, MnT5Hs, MnSNATs, MnASMT genes and MnCOMTs. Sample names are shown above the heat maps. The color scale indicates the degree of expression: "green" indicates low expression; "red" indicates high expression. B: Tissue-specific expression levels of MnTDC, MnT5H2, MnSNAT5, MnASMT12 and MnCOMT1 as determined by qRT-PCR. Data was analyzed using 2 ΔCt method and MnACTIN3 was used as an internal control. Data are means ±SDs (n = 3), significant differences (P <0.05) are indicated by different letters above the bars. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 1 in Genome-wide identification and characterization of genes involved in melatonin biosynthesis in Morus notabilis (wild mulberry)
Fig. 1. Phylogenetic tree of amino acid sequences of genes involved in melatonin biosynthesis in M. notabilis and other plants. The phylogenetic trees were generated with the neighbor-joining method using MEGA 5.0 software. All IDs of gene in other plant species involved in melatonin biosynthesis are provided in Supplementary Table S1. Red circles: M. notabilis genes. A, TDC; B, T5H; C, SNAT; D, ASMT. ASMT proteins were separated into three classes: I (black), II (red), III (blue); E, COMT. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 6 in Genome-wide identification and characterization of genes involved in melatonin biosynthesis in Morus notabilis (wild mulberry)
Fig. 6. Purification and enzymatic analyses of recombinant His-tagged MnCOMT1. A: Purification of recombinant His-tagged MnCOMT1 (lane1: Protein Marker; lane2: pCold TF (+IPTG); lane3: recombinant protein (-IPTG); lane4: recombinant protein (+IPTG); lane5: purified protein). B: Mass spectrum of compounds detected using UPLC-MS/MS. a: melatonin standard; b: Chromatogram of N-acetylserotonin catalyzed by MnCOMT1. C: Determination of Km and Vmax of MnCOMT1 for Nacetylserotonin. MnCOMT1 (1.0 μg) was co-incubated with different concentrations of N-acetylserotonin for 20 min at 37 ◦C. D: Mass spectrum of compounds detected using UPLC-MS/MS. a: 5-methoxytryptamine standard; b: Chromatogram of serotonin catalyzed by MnCOMT1. E: Determination of Km and Vmax of MnCOMT1 for serotonin. MnCOMT1 (1.0 μg) was co-incubated with different concentrations of serotonin for 20 min at 37 ◦C. " IPTG" stand without addition of 1 mM IPTG to the E. coli BL21 (DE3) culture, "+IPTG" stand addition of 1 mM IPTG to the E. coli BL21 (DE3) culture. "TF" is Trigger Factor, a prokaryotic ribosomerelated molecular chaperone that facilitates the translation and folding of peptides. "*" indicates Trigger factor.
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